Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription
Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct h...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Liu, Zheng [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Anmerkung: |
© The Genetics Society of Korea 2013 |
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| Übergeordnetes Werk: |
Enthalten in: Genes & Genomics - The Genetics Society of Korea, 2010, 36(2013), 1 vom: 26. Sept., Seite 95-103 |
|---|---|
| Übergeordnetes Werk: |
volume:36 ; year:2013 ; number:1 ; day:26 ; month:09 ; pages:95-103 |
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| DOI / URN: |
10.1007/s13258-013-0147-8 |
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| 520 | |a Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. | ||
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10.1007/s13258-013-0147-8 doi (DE-627)SPR031100414 (SPR)s13258-013-0147-8-e DE-627 ger DE-627 rakwb eng Liu, Zheng verfasserin aut Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2013 Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. Convergent transcription (dpeaa)DE-He213 RNA-directed DNA methylation (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 Transgene rearrangement (dpeaa)DE-He213 Gao, Shuangcheng aut Zhang, Shumin aut Yang, Shangjun aut Sun, Ning aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 36(2013), 1 vom: 26. Sept., Seite 95-103 (DE-627)SPR031096425 nnns volume:36 year:2013 number:1 day:26 month:09 pages:95-103 https://dx.doi.org/10.1007/s13258-013-0147-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 36 2013 1 26 09 95-103 |
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10.1007/s13258-013-0147-8 doi (DE-627)SPR031100414 (SPR)s13258-013-0147-8-e DE-627 ger DE-627 rakwb eng Liu, Zheng verfasserin aut Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2013 Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. Convergent transcription (dpeaa)DE-He213 RNA-directed DNA methylation (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 Transgene rearrangement (dpeaa)DE-He213 Gao, Shuangcheng aut Zhang, Shumin aut Yang, Shangjun aut Sun, Ning aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 36(2013), 1 vom: 26. Sept., Seite 95-103 (DE-627)SPR031096425 nnns volume:36 year:2013 number:1 day:26 month:09 pages:95-103 https://dx.doi.org/10.1007/s13258-013-0147-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 36 2013 1 26 09 95-103 |
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10.1007/s13258-013-0147-8 doi (DE-627)SPR031100414 (SPR)s13258-013-0147-8-e DE-627 ger DE-627 rakwb eng Liu, Zheng verfasserin aut Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2013 Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. Convergent transcription (dpeaa)DE-He213 RNA-directed DNA methylation (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 Transgene rearrangement (dpeaa)DE-He213 Gao, Shuangcheng aut Zhang, Shumin aut Yang, Shangjun aut Sun, Ning aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 36(2013), 1 vom: 26. Sept., Seite 95-103 (DE-627)SPR031096425 nnns volume:36 year:2013 number:1 day:26 month:09 pages:95-103 https://dx.doi.org/10.1007/s13258-013-0147-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 36 2013 1 26 09 95-103 |
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10.1007/s13258-013-0147-8 doi (DE-627)SPR031100414 (SPR)s13258-013-0147-8-e DE-627 ger DE-627 rakwb eng Liu, Zheng verfasserin aut Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2013 Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. Convergent transcription (dpeaa)DE-He213 RNA-directed DNA methylation (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 Transgene rearrangement (dpeaa)DE-He213 Gao, Shuangcheng aut Zhang, Shumin aut Yang, Shangjun aut Sun, Ning aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 36(2013), 1 vom: 26. Sept., Seite 95-103 (DE-627)SPR031096425 nnns volume:36 year:2013 number:1 day:26 month:09 pages:95-103 https://dx.doi.org/10.1007/s13258-013-0147-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 36 2013 1 26 09 95-103 |
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10.1007/s13258-013-0147-8 doi (DE-627)SPR031100414 (SPR)s13258-013-0147-8-e DE-627 ger DE-627 rakwb eng Liu, Zheng verfasserin aut Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2013 Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. Convergent transcription (dpeaa)DE-He213 RNA-directed DNA methylation (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 Transgene rearrangement (dpeaa)DE-He213 Gao, Shuangcheng aut Zhang, Shumin aut Yang, Shangjun aut Sun, Ning aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 36(2013), 1 vom: 26. Sept., Seite 95-103 (DE-627)SPR031096425 nnns volume:36 year:2013 number:1 day:26 month:09 pages:95-103 https://dx.doi.org/10.1007/s13258-013-0147-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 36 2013 1 26 09 95-103 |
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Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. © The Genetics Society of Korea 2013 |
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Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. © The Genetics Society of Korea 2013 |
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Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus. © The Genetics Society of Korea 2013 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR031100414</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230331065757.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2013 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s13258-013-0147-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR031100414</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s13258-013-0147-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Liu, Zheng</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Complex structures of transgene rearrangement implicate novel mechanisms of RNA-directed DNA methylation and convergent transcription</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2013</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Genetics Society of Korea 2013</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract An RNAi construct for silencing FtsZ gene functioning on plastid division in higher plants was transformed into tobacco genome. Sequencing of the flanking sequences of the T-DNA insertion site in a positive transgenic plant 2-3, which showed wildtype phenotype, revealed that the construct had rearranged, resulting in convergent transcription of nptII gene by 35S promoter and nos promoter with both transcripts expressing at low level. Although it is possible that the sense and antisense transcripts can form dsRNA, they did not trigger silencing of nptII gene derived from a FtsZ silenced plant 2-12 in the crossed plant 2-3×2-12. Furthermore, although small interfering RNA can trigger DNA methylation of the silencing locus, our investigation revealed that the crossed plant 2-3×2-12 showed partial methylation of the non-silencing transgene locus but full methylation of the non-rearranged silencing locus, suggesting that the efficiency of RNA-directed DNA methylation is associated with its original DNA locus.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Convergent transcription</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA-directed DNA methylation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA interference</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transgene rearrangement</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gao, Shuangcheng</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Shumin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, Shangjun</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Ning</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Genes & Genomics</subfield><subfield code="d">The Genetics Society of Korea, 2010</subfield><subfield code="g">36(2013), 1 vom: 26. Sept., Seite 95-103</subfield><subfield code="w">(DE-627)SPR031096425</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:36</subfield><subfield code="g">year:2013</subfield><subfield code="g">number:1</subfield><subfield code="g">day:26</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:95-103</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s13258-013-0147-8</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">36</subfield><subfield code="j">2013</subfield><subfield code="e">1</subfield><subfield code="b">26</subfield><subfield code="c">09</subfield><subfield code="h">95-103</subfield></datafield></record></collection>
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