Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Song, Liping [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© The Genetics Society of Korea 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Genes & Genomics - The Genetics Society of Korea, 2010, 41(2019), 12 vom: 01. Okt., Seite 1475-1492 |
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| Übergeordnetes Werk: |
volume:41 ; year:2019 ; number:12 ; day:01 ; month:10 ; pages:1475-1492 |
| Links: |
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| DOI / URN: |
10.1007/s13258-019-00867-x |
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| Katalog-ID: |
SPR031107346 |
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| 245 | 1 | 0 | |a Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A |
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| 520 | |a Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. | ||
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10.1007/s13258-019-00867-x doi (DE-627)SPR031107346 (SPR)s13258-019-00867-x-e DE-627 ger DE-627 rakwb eng Song, Liping verfasserin aut Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2019 Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 Li, Xia aut Zu, Feng aut Gao, Changbin aut Wang, Bincai aut Lin, Chufa aut Tu, Jinxing aut Wang, Aihua aut Zhou, Guolin (orcid)0000-0002-6923-3473 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 41(2019), 12 vom: 01. Okt., Seite 1475-1492 (DE-627)SPR031096425 nnns volume:41 year:2019 number:12 day:01 month:10 pages:1475-1492 https://dx.doi.org/10.1007/s13258-019-00867-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 41 2019 12 01 10 1475-1492 |
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10.1007/s13258-019-00867-x doi (DE-627)SPR031107346 (SPR)s13258-019-00867-x-e DE-627 ger DE-627 rakwb eng Song, Liping verfasserin aut Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2019 Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 Li, Xia aut Zu, Feng aut Gao, Changbin aut Wang, Bincai aut Lin, Chufa aut Tu, Jinxing aut Wang, Aihua aut Zhou, Guolin (orcid)0000-0002-6923-3473 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 41(2019), 12 vom: 01. Okt., Seite 1475-1492 (DE-627)SPR031096425 nnns volume:41 year:2019 number:12 day:01 month:10 pages:1475-1492 https://dx.doi.org/10.1007/s13258-019-00867-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 41 2019 12 01 10 1475-1492 |
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10.1007/s13258-019-00867-x doi (DE-627)SPR031107346 (SPR)s13258-019-00867-x-e DE-627 ger DE-627 rakwb eng Song, Liping verfasserin aut Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2019 Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 Li, Xia aut Zu, Feng aut Gao, Changbin aut Wang, Bincai aut Lin, Chufa aut Tu, Jinxing aut Wang, Aihua aut Zhou, Guolin (orcid)0000-0002-6923-3473 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 41(2019), 12 vom: 01. Okt., Seite 1475-1492 (DE-627)SPR031096425 nnns volume:41 year:2019 number:12 day:01 month:10 pages:1475-1492 https://dx.doi.org/10.1007/s13258-019-00867-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 41 2019 12 01 10 1475-1492 |
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10.1007/s13258-019-00867-x doi (DE-627)SPR031107346 (SPR)s13258-019-00867-x-e DE-627 ger DE-627 rakwb eng Song, Liping verfasserin aut Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2019 Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 Li, Xia aut Zu, Feng aut Gao, Changbin aut Wang, Bincai aut Lin, Chufa aut Tu, Jinxing aut Wang, Aihua aut Zhou, Guolin (orcid)0000-0002-6923-3473 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 41(2019), 12 vom: 01. Okt., Seite 1475-1492 (DE-627)SPR031096425 nnns volume:41 year:2019 number:12 day:01 month:10 pages:1475-1492 https://dx.doi.org/10.1007/s13258-019-00867-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 41 2019 12 01 10 1475-1492 |
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10.1007/s13258-019-00867-x doi (DE-627)SPR031107346 (SPR)s13258-019-00867-x-e DE-627 ger DE-627 rakwb eng Song, Liping verfasserin aut Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Genetics Society of Korea 2019 Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 Li, Xia aut Zu, Feng aut Gao, Changbin aut Wang, Bincai aut Lin, Chufa aut Tu, Jinxing aut Wang, Aihua aut Zhou, Guolin (orcid)0000-0002-6923-3473 aut Enthalten in Genes & Genomics The Genetics Society of Korea, 2010 41(2019), 12 vom: 01. Okt., Seite 1475-1492 (DE-627)SPR031096425 nnns volume:41 year:2019 number:12 day:01 month:10 pages:1475-1492 https://dx.doi.org/10.1007/s13258-019-00867-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 41 2019 12 01 10 1475-1492 |
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Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A Differentially expressed genes (dpeaa)DE-He213 Genic-male sterility (dpeaa)DE-He213 Non-heading Chinese cabbage (dpeaa)DE-He213 |
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misc Differentially expressed genes misc Genic-male sterility misc Non-heading Chinese cabbage |
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misc Differentially expressed genes misc Genic-male sterility misc Non-heading Chinese cabbage |
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Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A |
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Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A |
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Song, Liping |
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2019 |
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Song, Liping Li, Xia Zu, Feng Gao, Changbin Wang, Bincai Lin, Chufa Tu, Jinxing Wang, Aihua Zhou, Guolin |
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Elektronische Aufsätze |
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Song, Liping |
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10.1007/s13258-019-00867-x |
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comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading chinese cabbage (brassica rapa ssp. chinensis) line ws24-3a |
| title_auth |
Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A |
| abstract |
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. © The Genetics Society of Korea 2019 |
| abstractGer |
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. © The Genetics Society of Korea 2019 |
| abstract_unstemmed |
Background WS24-3A is a newly bred non-heading Chinese cabbage genic male-sterile line, in which sterility is controlled by a recessive gene, designated as Bra2ms. WS24-3A has been used for hybrid breeding. Objective To reveal the underlying molecular mechanisms responsible for the sterility of WS24-3A. Methods Cytological observation of the process of sterile/fertile anther development was performed to determine the tissue and stage in which sterility occurs. Phenotyping and transcriptomic analyses were performed to identify differentially expressed genes (DEGs) between sterile and fertile flower buds at different stages. Results Cytological analysis revealed no tetrads at stage 7 or at later stages of anther development, and the degradation of callose was delayed. Abnormal meiocytes were surrounded by sustaining callose that degenerated gradually in WS24-3A. Comparative transcript profiling identified 3282 DEGs during three anther developmental stages, namely, pre-meiotic anther, meiotic anther, and anthers with single-celled pollen stage. The difference in DEG percentage between up-regulated and down-regulated at meiotic anther stage was obviously larger than at the other two stages; further, most DEGs are important for male meiosis, callose synthesis and dissolution, and tapetum development. Ten DEGs were found to be involved in anther and pollen development, which were analyzed by quantitative PCR. Conclusion Bra2ms affected gene expression in meiocytes and associated with callose synthesis, degradation and tapetum development. Our results provide clues to elucidate the molecular mechanism of genic male sterility in non-heading Chinese cabbage. © The Genetics Society of Korea 2019 |
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12 |
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Comparative transcript profiling and cytological observation of the newly bred recessive genic male sterility non-heading Chinese cabbage (Brassica rapa ssp. chinensis) line WS24-3A |
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https://dx.doi.org/10.1007/s13258-019-00867-x |
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Li, Xia Zu, Feng Gao, Changbin Wang, Bincai Lin, Chufa Tu, Jinxing Wang, Aihua Zhou, Guolin |
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Li, Xia Zu, Feng Gao, Changbin Wang, Bincai Lin, Chufa Tu, Jinxing Wang, Aihua Zhou, Guolin |
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