Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3

Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibil...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Bertollo, Caryne Margotto [verfasserIn] Correa, Cristiane Rodrigues Gomes, Dawidson Assis Souza-Fagundes, Elaine Maria Goes, Alfredo Miranda

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Breast cancer Cell line Ionizing radiation Cell death DNA damage Caspase

Anmerkung:	© International Society of Oncology and BioMarkers (ISOBM) 2010

Übergeordnetes Werk:	Enthalten in: Tumor biology - Amsterdam : IOS Press, 1987, 31(2010), 3 vom: 27. Apr., Seite 189-197
Übergeordnetes Werk:	volume:31 ; year:2010 ; number:3 ; day:27 ; month:04 ; pages:189-197

Links:	Volltext

DOI / URN:	10.1007/s13277-010-0029-5

Katalog-ID:	SPR031120946

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allfields	10.1007/s13277-010-0029-5 doi (DE-627)SPR031120946 (SPR)s13277-010-0029-5-e DE-627 ger DE-627 rakwb eng Bertollo, Caryne Margotto verfasserin aut Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2010 Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213 Correa, Cristiane Rodrigues aut Gomes, Dawidson Assis aut Souza-Fagundes, Elaine Maria aut Goes, Alfredo Miranda aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 31(2010), 3 vom: 27. Apr., Seite 189-197 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:31 year:2010 number:3 day:27 month:04 pages:189-197 https://dx.doi.org/10.1007/s13277-010-0029-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 31 2010 3 27 04 189-197
spelling	10.1007/s13277-010-0029-5 doi (DE-627)SPR031120946 (SPR)s13277-010-0029-5-e DE-627 ger DE-627 rakwb eng Bertollo, Caryne Margotto verfasserin aut Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2010 Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213 Correa, Cristiane Rodrigues aut Gomes, Dawidson Assis aut Souza-Fagundes, Elaine Maria aut Goes, Alfredo Miranda aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 31(2010), 3 vom: 27. Apr., Seite 189-197 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:31 year:2010 number:3 day:27 month:04 pages:189-197 https://dx.doi.org/10.1007/s13277-010-0029-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 31 2010 3 27 04 189-197
allfields_unstemmed	10.1007/s13277-010-0029-5 doi (DE-627)SPR031120946 (SPR)s13277-010-0029-5-e DE-627 ger DE-627 rakwb eng Bertollo, Caryne Margotto verfasserin aut Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2010 Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213 Correa, Cristiane Rodrigues aut Gomes, Dawidson Assis aut Souza-Fagundes, Elaine Maria aut Goes, Alfredo Miranda aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 31(2010), 3 vom: 27. Apr., Seite 189-197 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:31 year:2010 number:3 day:27 month:04 pages:189-197 https://dx.doi.org/10.1007/s13277-010-0029-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 31 2010 3 27 04 189-197
allfieldsGer	10.1007/s13277-010-0029-5 doi (DE-627)SPR031120946 (SPR)s13277-010-0029-5-e DE-627 ger DE-627 rakwb eng Bertollo, Caryne Margotto verfasserin aut Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2010 Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213 Correa, Cristiane Rodrigues aut Gomes, Dawidson Assis aut Souza-Fagundes, Elaine Maria aut Goes, Alfredo Miranda aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 31(2010), 3 vom: 27. Apr., Seite 189-197 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:31 year:2010 number:3 day:27 month:04 pages:189-197 https://dx.doi.org/10.1007/s13277-010-0029-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 31 2010 3 27 04 189-197
allfieldsSound	10.1007/s13277-010-0029-5 doi (DE-627)SPR031120946 (SPR)s13277-010-0029-5-e DE-627 ger DE-627 rakwb eng Bertollo, Caryne Margotto verfasserin aut Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2010 Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213 Correa, Cristiane Rodrigues aut Gomes, Dawidson Assis aut Souza-Fagundes, Elaine Maria aut Goes, Alfredo Miranda aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 31(2010), 3 vom: 27. Apr., Seite 189-197 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:31 year:2010 number:3 day:27 month:04 pages:189-197 https://dx.doi.org/10.1007/s13277-010-0029-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 31 2010 3 27 04 189-197
language	English
source	Enthalten in Tumor biology 31(2010), 3 vom: 27. Apr., Seite 189-197 volume:31 year:2010 number:3 day:27 month:04 pages:189-197
sourceStr	Enthalten in Tumor biology 31(2010), 3 vom: 27. Apr., Seite 189-197 volume:31 year:2010 number:3 day:27 month:04 pages:189-197
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Breast cancer Cell line Ionizing radiation Cell death DNA damage Caspase
isfreeaccess_bool	false
container_title	Tumor biology
authorswithroles_txt_mv	Bertollo, Caryne Margotto @@aut@@ Correa, Cristiane Rodrigues @@aut@@ Gomes, Dawidson Assis @@aut@@ Souza-Fagundes, Elaine Maria @@aut@@ Goes, Alfredo Miranda @@aut@@
publishDateDaySort_date	2010-04-27T00:00:00Z
hierarchy_top_id	300897855
id	SPR031120946
language_de	englisch
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author	Bertollo, Caryne Margotto
spellingShingle	Bertollo, Caryne Margotto misc Breast cancer misc Cell line misc Ionizing radiation misc Cell death misc DNA damage misc Caspase Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3
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topic_title	Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3 Breast cancer (dpeaa)DE-He213 Cell line (dpeaa)DE-He213 Ionizing radiation (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 DNA damage (dpeaa)DE-He213 Caspase (dpeaa)DE-He213
topic	misc Breast cancer misc Cell line misc Ionizing radiation misc Cell death misc DNA damage misc Caspase
topic_unstemmed	misc Breast cancer misc Cell line misc Ionizing radiation misc Cell death misc DNA damage misc Caspase
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title	Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3
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title_full	Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3
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title_sort	effect of radiation treatment on newly established human breast cancer cell lines macl-1 and mgso-3
title_auth	Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3
abstract	Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. © International Society of Oncology and BioMarkers (ISOBM) 2010
abstractGer	Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. © International Society of Oncology and BioMarkers (ISOBM) 2010
abstract_unstemmed	Abstract The characterization of new cell lines is an important tool to understand the biological processes involved in cancer treatments. In the present study, we used two newly established epithelial human breast cancer cell lines from primary sites MACL-1 and MGSO-3 and compared their susceptibility to the treatment with ionizing radiation (IR) with the commercial cell line MDA-MB-231. In the doses used (10 or 20 Gy), IR induced a reduction in cell viability and cell death, measured as DNA fragmentation, at 48 and 72 h after treatment. In addition, 48 h after treatment with IR, we observed an enhancement in the percentage of apoptotic cells. The broad-range caspases inhibitor zVAD-FMK inhibited cytotoxicity induced by IR. After 24 h, treatment with IR activated caspase-9 in MACL-1 and MDA-MB-231 but not in MGSO-3 cells. Thirty hours after treatment with IR (20 Gy), we observed an activation of caspases 8 and 3. These results suggest the involvement of caspases in the cell death induced by IR in two newly established cell lines. These cells may be useful in studies of breast cancer in defining basic mechanisms in molecular and cellular radiobiology and may contribute to the rational design of future models of cancer therapies. © International Society of Oncology and BioMarkers (ISOBM) 2010
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title_short	Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3
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Effect of radiation treatment on newly established human breast cancer cell lines MACL-1 and MGSO-3

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