MKK4 acts as a potential tumor suppressor in ovarian cancer

Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4...
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Autor*in:	Yeasmin, Shamima [verfasserIn] Nakayama, Kentaro Rahman, Mohammed Tanjimur Rahman, Munmun Ishikawa, Masako Katagiri, Atsuko Iida, Kouji Nakayama, Naomi Miyazaki, Kohji

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Schlagwörter:	MKK4 Tumor suppressor gene Ovarian cancer

Anmerkung:	© International Society of Oncology and BioMarkers (ISOBM) 2011

Übergeordnetes Werk:	Enthalten in: Tumor biology - Amsterdam : IOS Press, 1987, 32(2011), 4 vom: 14. Apr., Seite 661-670
Übergeordnetes Werk:	volume:32 ; year:2011 ; number:4 ; day:14 ; month:04 ; pages:661-670

Links:	Volltext

DOI / URN:	10.1007/s13277-011-0166-5

Katalog-ID:	SPR031122477

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matchkey_str	article:14230380:2011----::k4cssptniluosprsoi
hierarchy_sort_str	2011
publishDate	2011
allfields	10.1007/s13277-011-0166-5 doi (DE-627)SPR031122477 (SPR)s13277-011-0166-5-e DE-627 ger DE-627 rakwb eng Yeasmin, Shamima verfasserin aut MKK4 acts as a potential tumor suppressor in ovarian cancer 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2011 Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213 Nakayama, Kentaro aut Rahman, Mohammed Tanjimur aut Rahman, Munmun aut Ishikawa, Masako aut Katagiri, Atsuko aut Iida, Kouji aut Nakayama, Naomi aut Miyazaki, Kohji aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 32(2011), 4 vom: 14. Apr., Seite 661-670 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:32 year:2011 number:4 day:14 month:04 pages:661-670 https://dx.doi.org/10.1007/s13277-011-0166-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 32 2011 4 14 04 661-670
spelling	10.1007/s13277-011-0166-5 doi (DE-627)SPR031122477 (SPR)s13277-011-0166-5-e DE-627 ger DE-627 rakwb eng Yeasmin, Shamima verfasserin aut MKK4 acts as a potential tumor suppressor in ovarian cancer 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2011 Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213 Nakayama, Kentaro aut Rahman, Mohammed Tanjimur aut Rahman, Munmun aut Ishikawa, Masako aut Katagiri, Atsuko aut Iida, Kouji aut Nakayama, Naomi aut Miyazaki, Kohji aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 32(2011), 4 vom: 14. Apr., Seite 661-670 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:32 year:2011 number:4 day:14 month:04 pages:661-670 https://dx.doi.org/10.1007/s13277-011-0166-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 32 2011 4 14 04 661-670
allfields_unstemmed	10.1007/s13277-011-0166-5 doi (DE-627)SPR031122477 (SPR)s13277-011-0166-5-e DE-627 ger DE-627 rakwb eng Yeasmin, Shamima verfasserin aut MKK4 acts as a potential tumor suppressor in ovarian cancer 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2011 Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213 Nakayama, Kentaro aut Rahman, Mohammed Tanjimur aut Rahman, Munmun aut Ishikawa, Masako aut Katagiri, Atsuko aut Iida, Kouji aut Nakayama, Naomi aut Miyazaki, Kohji aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 32(2011), 4 vom: 14. Apr., Seite 661-670 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:32 year:2011 number:4 day:14 month:04 pages:661-670 https://dx.doi.org/10.1007/s13277-011-0166-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 32 2011 4 14 04 661-670
allfieldsGer	10.1007/s13277-011-0166-5 doi (DE-627)SPR031122477 (SPR)s13277-011-0166-5-e DE-627 ger DE-627 rakwb eng Yeasmin, Shamima verfasserin aut MKK4 acts as a potential tumor suppressor in ovarian cancer 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2011 Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213 Nakayama, Kentaro aut Rahman, Mohammed Tanjimur aut Rahman, Munmun aut Ishikawa, Masako aut Katagiri, Atsuko aut Iida, Kouji aut Nakayama, Naomi aut Miyazaki, Kohji aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 32(2011), 4 vom: 14. Apr., Seite 661-670 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:32 year:2011 number:4 day:14 month:04 pages:661-670 https://dx.doi.org/10.1007/s13277-011-0166-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 32 2011 4 14 04 661-670
allfieldsSound	10.1007/s13277-011-0166-5 doi (DE-627)SPR031122477 (SPR)s13277-011-0166-5-e DE-627 ger DE-627 rakwb eng Yeasmin, Shamima verfasserin aut MKK4 acts as a potential tumor suppressor in ovarian cancer 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © International Society of Oncology and BioMarkers (ISOBM) 2011 Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213 Nakayama, Kentaro aut Rahman, Mohammed Tanjimur aut Rahman, Munmun aut Ishikawa, Masako aut Katagiri, Atsuko aut Iida, Kouji aut Nakayama, Naomi aut Miyazaki, Kohji aut Enthalten in Tumor biology Amsterdam : IOS Press, 1987 32(2011), 4 vom: 14. Apr., Seite 661-670 (DE-627)300897855 (DE-600)1483579-4 1423-0380 nnns volume:32 year:2011 number:4 day:14 month:04 pages:661-670 https://dx.doi.org/10.1007/s13277-011-0166-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_22 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_63 GBV_ILN_95 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_370 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4338 AR 32 2011 4 14 04 661-670
language	English
source	Enthalten in Tumor biology 32(2011), 4 vom: 14. Apr., Seite 661-670 volume:32 year:2011 number:4 day:14 month:04 pages:661-670
sourceStr	Enthalten in Tumor biology 32(2011), 4 vom: 14. Apr., Seite 661-670 volume:32 year:2011 number:4 day:14 month:04 pages:661-670
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	MKK4 Tumor suppressor gene Ovarian cancer
isfreeaccess_bool	false
container_title	Tumor biology
authorswithroles_txt_mv	Yeasmin, Shamima @@aut@@ Nakayama, Kentaro @@aut@@ Rahman, Mohammed Tanjimur @@aut@@ Rahman, Munmun @@aut@@ Ishikawa, Masako @@aut@@ Katagiri, Atsuko @@aut@@ Iida, Kouji @@aut@@ Nakayama, Naomi @@aut@@ Miyazaki, Kohji @@aut@@
publishDateDaySort_date	2011-04-14T00:00:00Z
hierarchy_top_id	300897855
id	SPR031122477
language_de	englisch
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author	Yeasmin, Shamima
spellingShingle	Yeasmin, Shamima misc MKK4 misc Tumor suppressor gene misc Ovarian cancer MKK4 acts as a potential tumor suppressor in ovarian cancer
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topic_title	MKK4 acts as a potential tumor suppressor in ovarian cancer MKK4 (dpeaa)DE-He213 Tumor suppressor gene (dpeaa)DE-He213 Ovarian cancer (dpeaa)DE-He213
topic	misc MKK4 misc Tumor suppressor gene misc Ovarian cancer
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title	MKK4 acts as a potential tumor suppressor in ovarian cancer
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title_full	MKK4 acts as a potential tumor suppressor in ovarian cancer
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author_browse	Yeasmin, Shamima Nakayama, Kentaro Rahman, Mohammed Tanjimur Rahman, Munmun Ishikawa, Masako Katagiri, Atsuko Iida, Kouji Nakayama, Naomi Miyazaki, Kohji
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title_sort	mkk4 acts as a potential tumor suppressor in ovarian cancer
title_auth	MKK4 acts as a potential tumor suppressor in ovarian cancer
abstract	Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. © International Society of Oncology and BioMarkers (ISOBM) 2011
abstractGer	Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. © International Society of Oncology and BioMarkers (ISOBM) 2011
abstract_unstemmed	Abstract Our previous studies indicate that loss of MKK4 expression is associated with the progression of ovarian cancer. However, direct evidence that MKK4 inhibits the malignant phenotype of ovarian cancer cells is limited. In the current study, we investigated the mechanism relating loss of MKK4 expression to the development of ovarian cancer. Using cell growth and anchorage-independent assays, we determined that both the growth and colony-forming ability of MKK4-transfected TOV-21G cells, a line with a homozygous deletion of MKK4, were significantly reduced compared to control vector-transfected cells. Overexpression of the MKK4 gene in TOV-21G cells resulted in reduced proliferative activity and increased apoptosis. To confirm that MKK4 expression related to tumor suppress function, we used two independent but complementary approaches. MKK4 gene knockdown in OVK18#2 and MDAH2774 cells, which overexpressed MKK4, increased proliferation activity. Additionally, the engineered expression of MKK4 in SKOV3 cells, a line with low endogenous MKK4 expression, produced a phenotype similar to that of TOV-21G. Similar results were produced in tumor xenografts in nude mice. These results indicated that MKK4 acts as a tumor suppressor and may represent an important therapeutic target for the treatment of ovarian cancer. © International Society of Oncology and BioMarkers (ISOBM) 2011
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title_short	MKK4 acts as a potential tumor suppressor in ovarian cancer
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MKK4 acts as a potential tumor suppressor in ovarian cancer

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