Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims
Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purp...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
García-Gutiérrez, María S. [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Anmerkung: |
© The American Society for Experimental NeuroTherapeutics, Inc. 2018 |
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| Übergeordnetes Werk: |
Enthalten in: NeuroRX - Springer-Verlag, 2006, 15(2018), 3 vom: 12. Feb., Seite 796-806 |
|---|---|
| Übergeordnetes Werk: |
volume:15 ; year:2018 ; number:3 ; day:12 ; month:02 ; pages:796-806 |
| Links: |
|---|
| DOI / URN: |
10.1007/s13311-018-0610-y |
|---|
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SPR031272835 |
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| 520 | |a Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. | ||
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10.1007/s13311-018-0610-y doi (DE-627)SPR031272835 (SPR)s13311-018-0610-y-e DE-627 ger DE-627 rakwb eng García-Gutiérrez, María S. verfasserin aut Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The American Society for Experimental NeuroTherapeutics, Inc. 2018 Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 Navarrete, Francisco aut Navarro, Gemma aut Reyes-Resina, Irene aut Franco, Rafael aut Lanciego, Jose Luis aut Giner, Salvador aut Manzanares, Jorge (orcid)0000-0002-4681-1533 aut Enthalten in NeuroRX Springer-Verlag, 2006 15(2018), 3 vom: 12. Feb., Seite 796-806 (DE-627)SPR031264964 nnns volume:15 year:2018 number:3 day:12 month:02 pages:796-806 https://dx.doi.org/10.1007/s13311-018-0610-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 15 2018 3 12 02 796-806 |
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10.1007/s13311-018-0610-y doi (DE-627)SPR031272835 (SPR)s13311-018-0610-y-e DE-627 ger DE-627 rakwb eng García-Gutiérrez, María S. verfasserin aut Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The American Society for Experimental NeuroTherapeutics, Inc. 2018 Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 Navarrete, Francisco aut Navarro, Gemma aut Reyes-Resina, Irene aut Franco, Rafael aut Lanciego, Jose Luis aut Giner, Salvador aut Manzanares, Jorge (orcid)0000-0002-4681-1533 aut Enthalten in NeuroRX Springer-Verlag, 2006 15(2018), 3 vom: 12. Feb., Seite 796-806 (DE-627)SPR031264964 nnns volume:15 year:2018 number:3 day:12 month:02 pages:796-806 https://dx.doi.org/10.1007/s13311-018-0610-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 15 2018 3 12 02 796-806 |
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10.1007/s13311-018-0610-y doi (DE-627)SPR031272835 (SPR)s13311-018-0610-y-e DE-627 ger DE-627 rakwb eng García-Gutiérrez, María S. verfasserin aut Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The American Society for Experimental NeuroTherapeutics, Inc. 2018 Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 Navarrete, Francisco aut Navarro, Gemma aut Reyes-Resina, Irene aut Franco, Rafael aut Lanciego, Jose Luis aut Giner, Salvador aut Manzanares, Jorge (orcid)0000-0002-4681-1533 aut Enthalten in NeuroRX Springer-Verlag, 2006 15(2018), 3 vom: 12. Feb., Seite 796-806 (DE-627)SPR031264964 nnns volume:15 year:2018 number:3 day:12 month:02 pages:796-806 https://dx.doi.org/10.1007/s13311-018-0610-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 15 2018 3 12 02 796-806 |
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10.1007/s13311-018-0610-y doi (DE-627)SPR031272835 (SPR)s13311-018-0610-y-e DE-627 ger DE-627 rakwb eng García-Gutiérrez, María S. verfasserin aut Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The American Society for Experimental NeuroTherapeutics, Inc. 2018 Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 Navarrete, Francisco aut Navarro, Gemma aut Reyes-Resina, Irene aut Franco, Rafael aut Lanciego, Jose Luis aut Giner, Salvador aut Manzanares, Jorge (orcid)0000-0002-4681-1533 aut Enthalten in NeuroRX Springer-Verlag, 2006 15(2018), 3 vom: 12. Feb., Seite 796-806 (DE-627)SPR031264964 nnns volume:15 year:2018 number:3 day:12 month:02 pages:796-806 https://dx.doi.org/10.1007/s13311-018-0610-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 15 2018 3 12 02 796-806 |
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10.1007/s13311-018-0610-y doi (DE-627)SPR031272835 (SPR)s13311-018-0610-y-e DE-627 ger DE-627 rakwb eng García-Gutiérrez, María S. verfasserin aut Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The American Society for Experimental NeuroTherapeutics, Inc. 2018 Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 Navarrete, Francisco aut Navarro, Gemma aut Reyes-Resina, Irene aut Franco, Rafael aut Lanciego, Jose Luis aut Giner, Salvador aut Manzanares, Jorge (orcid)0000-0002-4681-1533 aut Enthalten in NeuroRX Springer-Verlag, 2006 15(2018), 3 vom: 12. Feb., Seite 796-806 (DE-627)SPR031264964 nnns volume:15 year:2018 number:3 day:12 month:02 pages:796-806 https://dx.doi.org/10.1007/s13311-018-0610-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 15 2018 3 12 02 796-806 |
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García-Gutiérrez, María S. |
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García-Gutiérrez, María S. misc CB misc r misc GPR55 misc Suicide misc Dorsolateral prefrontal cortex misc Gene expression misc In situ proximity ligation assay Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims |
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Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims CB (dpeaa)DE-He213 r (dpeaa)DE-He213 GPR55 (dpeaa)DE-He213 Suicide (dpeaa)DE-He213 Dorsolateral prefrontal cortex (dpeaa)DE-He213 Gene expression (dpeaa)DE-He213 In situ proximity ligation assay (dpeaa)DE-He213 |
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alterations in gene and protein expression of cannabinoid $ cb_{2} $ and gpr55 receptors in the dorsolateral prefrontal cortex of suicide victims |
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Alterations in Gene and Protein Expression of Cannabinoid $ CB_{2} $ and GPR55 Receptors in the Dorsolateral Prefrontal Cortex of Suicide Victims |
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Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. © The American Society for Experimental NeuroTherapeutics, Inc. 2018 |
| abstractGer |
Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. © The American Society for Experimental NeuroTherapeutics, Inc. 2018 |
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Abstract Recent studies point to the cannabinoid $ CB_{2} $ receptors ($ CB_{2} $r) and the non-cannabinoid receptor GPR55 as potential key targets involved in the response to stress, anxiety, and depression. Considering the close relationship between neuropsychiatric disorders and suicide, the purpose of this study was to evaluate the potential alterations of $ CB_{2} $r and GPR55 in suicide victims. We analyzed gene and protein expression of both receptors by real-time PCR and western blot, respectively, in the dorsolateral prefrontal cortex (DLPFC) of 18 suicide victims with no clinical psychiatric history or treatment with anxiolytics or antidepressants, and 15 corresponding controls. We used in situ proximity ligation assay to evaluate whether the receptors formed heteromeric complexes and to determine the expression level of these heteromers, also assessing the co-expression of heteromers in neurons, astroglia, or microglia cells. $ CB_{2} $r and GPR55 gene expressions were significantly lower (by 33 and 41%, respectively) in the DLPFC of suicide cases. $ CB_{2} $r protein expression was higher, as were $ CB_{2} $-GPR55 heteroreceptor complexes. The results also revealed the presence of $ CB_{2} $-GPR55 receptor heteromers in both neurons and astrocytes, whereas microglial cells showed no expression. We did not observe any significant alterations of GPR55 protein expression. Additional studies will be necessary to evaluate if these alterations are reproducible in suicide victims diagnosed with different psychiatric disorders. Taken together, the results suggest that $ CB_{2} $r and GPR55 may play a relevant role in the neurobiology of suicide. © The American Society for Experimental NeuroTherapeutics, Inc. 2018 |
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