Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya
Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples...
Ausführliche Beschreibung
Autor*in: |
Chakraborty, A. K. [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2018 |
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Anmerkung: |
© Indian Virological Society 2018 |
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Übergeordnetes Werk: |
Enthalten in: Indian journal of virology - [New Delhi] : Springer India, 2010, 29(2018), 1 vom: 17. Feb., Seite 89-95 |
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Übergeordnetes Werk: |
volume:29 ; year:2018 ; number:1 ; day:17 ; month:02 ; pages:89-95 |
Links: |
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DOI / URN: |
10.1007/s13337-018-0433-9 |
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Katalog-ID: |
SPR031384285 |
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10.1007/s13337-018-0433-9 doi (DE-627)SPR031384285 (SPR)s13337-018-0433-9-e DE-627 ger DE-627 rakwb eng Chakraborty, A. K. verfasserin aut Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Virological Society 2018 Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. 5′UTR (dpeaa)DE-He213 CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 I-TASSER (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 Karam, A. aut Mukherjee, P. aut Barkalita, L. aut Borah, P. aut Das, S. aut Sanjukta, R. aut Puro, K. aut Ghatak, S. aut Shakuntala, I. aut Sharma, I. aut Laha, R. G. aut Sen, A. aut Enthalten in Indian journal of virology [New Delhi] : Springer India, 2010 29(2018), 1 vom: 17. Feb., Seite 89-95 (DE-627)635133717 (DE-600)2572261-X 0974-0120 nnns volume:29 year:2018 number:1 day:17 month:02 pages:89-95 https://dx.doi.org/10.1007/s13337-018-0433-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_70 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 AR 29 2018 1 17 02 89-95 |
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10.1007/s13337-018-0433-9 doi (DE-627)SPR031384285 (SPR)s13337-018-0433-9-e DE-627 ger DE-627 rakwb eng Chakraborty, A. K. verfasserin aut Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Virological Society 2018 Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. 5′UTR (dpeaa)DE-He213 CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 I-TASSER (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 Karam, A. aut Mukherjee, P. aut Barkalita, L. aut Borah, P. aut Das, S. aut Sanjukta, R. aut Puro, K. aut Ghatak, S. aut Shakuntala, I. aut Sharma, I. aut Laha, R. G. aut Sen, A. aut Enthalten in Indian journal of virology [New Delhi] : Springer India, 2010 29(2018), 1 vom: 17. Feb., Seite 89-95 (DE-627)635133717 (DE-600)2572261-X 0974-0120 nnns volume:29 year:2018 number:1 day:17 month:02 pages:89-95 https://dx.doi.org/10.1007/s13337-018-0433-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_70 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 AR 29 2018 1 17 02 89-95 |
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10.1007/s13337-018-0433-9 doi (DE-627)SPR031384285 (SPR)s13337-018-0433-9-e DE-627 ger DE-627 rakwb eng Chakraborty, A. K. verfasserin aut Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Virological Society 2018 Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. 5′UTR (dpeaa)DE-He213 CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 I-TASSER (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 Karam, A. aut Mukherjee, P. aut Barkalita, L. aut Borah, P. aut Das, S. aut Sanjukta, R. aut Puro, K. aut Ghatak, S. aut Shakuntala, I. aut Sharma, I. aut Laha, R. G. aut Sen, A. aut Enthalten in Indian journal of virology [New Delhi] : Springer India, 2010 29(2018), 1 vom: 17. Feb., Seite 89-95 (DE-627)635133717 (DE-600)2572261-X 0974-0120 nnns volume:29 year:2018 number:1 day:17 month:02 pages:89-95 https://dx.doi.org/10.1007/s13337-018-0433-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_70 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 AR 29 2018 1 17 02 89-95 |
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10.1007/s13337-018-0433-9 doi (DE-627)SPR031384285 (SPR)s13337-018-0433-9-e DE-627 ger DE-627 rakwb eng Chakraborty, A. K. verfasserin aut Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Virological Society 2018 Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. 5′UTR (dpeaa)DE-He213 CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 I-TASSER (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 Karam, A. aut Mukherjee, P. aut Barkalita, L. aut Borah, P. aut Das, S. aut Sanjukta, R. aut Puro, K. aut Ghatak, S. aut Shakuntala, I. aut Sharma, I. aut Laha, R. G. aut Sen, A. aut Enthalten in Indian journal of virology [New Delhi] : Springer India, 2010 29(2018), 1 vom: 17. Feb., Seite 89-95 (DE-627)635133717 (DE-600)2572261-X 0974-0120 nnns volume:29 year:2018 number:1 day:17 month:02 pages:89-95 https://dx.doi.org/10.1007/s13337-018-0433-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_70 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 AR 29 2018 1 17 02 89-95 |
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10.1007/s13337-018-0433-9 doi (DE-627)SPR031384285 (SPR)s13337-018-0433-9-e DE-627 ger DE-627 rakwb eng Chakraborty, A. K. verfasserin aut Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Virological Society 2018 Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. 5′UTR (dpeaa)DE-He213 CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 I-TASSER (dpeaa)DE-He213 RT-PCR (dpeaa)DE-He213 Karam, A. aut Mukherjee, P. aut Barkalita, L. aut Borah, P. aut Das, S. aut Sanjukta, R. aut Puro, K. aut Ghatak, S. aut Shakuntala, I. aut Sharma, I. aut Laha, R. G. aut Sen, A. aut Enthalten in Indian journal of virology [New Delhi] : Springer India, 2010 29(2018), 1 vom: 17. Feb., Seite 89-95 (DE-627)635133717 (DE-600)2572261-X 0974-0120 nnns volume:29 year:2018 number:1 day:17 month:02 pages:89-95 https://dx.doi.org/10.1007/s13337-018-0433-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_70 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 AR 29 2018 1 17 02 89-95 |
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Chakraborty, A. K. misc 5′UTR misc CSFV misc E2 misc I-TASSER misc RT-PCR Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya |
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detection of classical swine fever virus e2 gene in cattle serum samples from cattle herds of meghalaya |
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Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya |
abstract |
Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. © Indian Virological Society 2018 |
abstractGer |
Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. © Indian Virological Society 2018 |
abstract_unstemmed |
Abstract The present study focused on the detection and genetic characterisation of 5′ untranslated region (5′UTR) and E2 gene of classical swine fever virus (CSFV, family Flaviviridae, genus Pestivirus) from bovine population of the northeastern region of India. A total of 134 cattle serum samples were collected from organised cattle farms and were screened for CSFV antigen with a commercial antigen capture enzyme linked immunosorbent assay (Ag-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). A total of 10 samples were positive for CSFV antigen by ELISA, while all of them were positive in PCR for 5′UTR region. Full length E2 region of CSFV were successfully amplified from two positive samples and used for subsequent phylogenetic analysis and determination of protein 3D structure which showed similarity with reported CSFV isolate from Assam of sub-genogroup 2.1, with minor variations in protein structure. © Indian Virological Society 2018 |
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Detection of classical swine fever virus E2 gene in cattle serum samples from cattle herds of Meghalaya |
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