Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin
Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histolo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Akarca, Saadet Özen [verfasserIn] Yavaşoğlu, Altuğ [verfasserIn] Ayşegül, Uysal [verfasserIn] Fatih, Oltulu [verfasserIn] Yılmaz-Dilsiz, Özlem [verfasserIn] Timur, Köse [verfasserIn] Hüseyin, Aktuğ [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: International journal of diabetes in developing countries - [Delhi] : Springer India, 2006, 32(2012), 2 vom: Juni, Seite 82-89 |
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| Übergeordnetes Werk: |
volume:32 ; year:2012 ; number:2 ; month:06 ; pages:82-89 |
| Links: |
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| DOI / URN: |
10.1007/s13410-012-0070-6 |
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| Katalog-ID: |
SPR031600271 |
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| 520 | |a Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. | ||
| 650 | 4 | |a Diabetes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Skin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Basement membrane |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Type IV collagen |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Connexin 43 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Epidermal thickness |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Yavaşoğlu, Altuğ |e verfasserin |4 aut | |
| 700 | 1 | |a Ayşegül, Uysal |e verfasserin |4 aut | |
| 700 | 1 | |a Fatih, Oltulu |e verfasserin |4 aut | |
| 700 | 1 | |a Yılmaz-Dilsiz, Özlem |e verfasserin |4 aut | |
| 700 | 1 | |a Timur, Köse |e verfasserin |4 aut | |
| 700 | 1 | |a Hüseyin, Aktuğ |e verfasserin |4 aut | |
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10.1007/s13410-012-0070-6 doi (DE-627)SPR031600271 (SPR)s13410-012-0070-6-e DE-627 ger DE-627 rakwb eng 610 ASE Akarca, Saadet Özen verfasserin aut Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 Yavaşoğlu, Altuğ verfasserin aut Ayşegül, Uysal verfasserin aut Fatih, Oltulu verfasserin aut Yılmaz-Dilsiz, Özlem verfasserin aut Timur, Köse verfasserin aut Hüseyin, Aktuğ verfasserin aut Enthalten in International journal of diabetes in developing countries [Delhi] : Springer India, 2006 32(2012), 2 vom: Juni, Seite 82-89 (DE-627)521483700 (DE-600)2263351-0 1998-3832 nnns volume:32 year:2012 number:2 month:06 pages:82-89 https://dx.doi.org/10.1007/s13410-012-0070-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 32 2012 2 06 82-89 |
| spelling |
10.1007/s13410-012-0070-6 doi (DE-627)SPR031600271 (SPR)s13410-012-0070-6-e DE-627 ger DE-627 rakwb eng 610 ASE Akarca, Saadet Özen verfasserin aut Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 Yavaşoğlu, Altuğ verfasserin aut Ayşegül, Uysal verfasserin aut Fatih, Oltulu verfasserin aut Yılmaz-Dilsiz, Özlem verfasserin aut Timur, Köse verfasserin aut Hüseyin, Aktuğ verfasserin aut Enthalten in International journal of diabetes in developing countries [Delhi] : Springer India, 2006 32(2012), 2 vom: Juni, Seite 82-89 (DE-627)521483700 (DE-600)2263351-0 1998-3832 nnns volume:32 year:2012 number:2 month:06 pages:82-89 https://dx.doi.org/10.1007/s13410-012-0070-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 32 2012 2 06 82-89 |
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10.1007/s13410-012-0070-6 doi (DE-627)SPR031600271 (SPR)s13410-012-0070-6-e DE-627 ger DE-627 rakwb eng 610 ASE Akarca, Saadet Özen verfasserin aut Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 Yavaşoğlu, Altuğ verfasserin aut Ayşegül, Uysal verfasserin aut Fatih, Oltulu verfasserin aut Yılmaz-Dilsiz, Özlem verfasserin aut Timur, Köse verfasserin aut Hüseyin, Aktuğ verfasserin aut Enthalten in International journal of diabetes in developing countries [Delhi] : Springer India, 2006 32(2012), 2 vom: Juni, Seite 82-89 (DE-627)521483700 (DE-600)2263351-0 1998-3832 nnns volume:32 year:2012 number:2 month:06 pages:82-89 https://dx.doi.org/10.1007/s13410-012-0070-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 32 2012 2 06 82-89 |
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10.1007/s13410-012-0070-6 doi (DE-627)SPR031600271 (SPR)s13410-012-0070-6-e DE-627 ger DE-627 rakwb eng 610 ASE Akarca, Saadet Özen verfasserin aut Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 Yavaşoğlu, Altuğ verfasserin aut Ayşegül, Uysal verfasserin aut Fatih, Oltulu verfasserin aut Yılmaz-Dilsiz, Özlem verfasserin aut Timur, Köse verfasserin aut Hüseyin, Aktuğ verfasserin aut Enthalten in International journal of diabetes in developing countries [Delhi] : Springer India, 2006 32(2012), 2 vom: Juni, Seite 82-89 (DE-627)521483700 (DE-600)2263351-0 1998-3832 nnns volume:32 year:2012 number:2 month:06 pages:82-89 https://dx.doi.org/10.1007/s13410-012-0070-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 32 2012 2 06 82-89 |
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10.1007/s13410-012-0070-6 doi (DE-627)SPR031600271 (SPR)s13410-012-0070-6-e DE-627 ger DE-627 rakwb eng 610 ASE Akarca, Saadet Özen verfasserin aut Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin 2012 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 Yavaşoğlu, Altuğ verfasserin aut Ayşegül, Uysal verfasserin aut Fatih, Oltulu verfasserin aut Yılmaz-Dilsiz, Özlem verfasserin aut Timur, Köse verfasserin aut Hüseyin, Aktuğ verfasserin aut Enthalten in International journal of diabetes in developing countries [Delhi] : Springer India, 2006 32(2012), 2 vom: Juni, Seite 82-89 (DE-627)521483700 (DE-600)2263351-0 1998-3832 nnns volume:32 year:2012 number:2 month:06 pages:82-89 https://dx.doi.org/10.1007/s13410-012-0070-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 32 2012 2 06 82-89 |
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Enthalten in International journal of diabetes in developing countries 32(2012), 2 vom: Juni, Seite 82-89 volume:32 year:2012 number:2 month:06 pages:82-89 |
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Akarca, Saadet Özen @@aut@@ Yavaşoğlu, Altuğ @@aut@@ Ayşegül, Uysal @@aut@@ Fatih, Oltulu @@aut@@ Yılmaz-Dilsiz, Özlem @@aut@@ Timur, Köse @@aut@@ Hüseyin, Aktuğ @@aut@@ |
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Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. 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Akarca, Saadet Özen |
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Akarca, Saadet Özen ddc 610 misc Diabetes misc Skin misc Basement membrane misc Type IV collagen misc Connexin 43 misc Epidermal thickness Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin |
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1998-3832 |
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610 ASE Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin Diabetes (dpeaa)DE-He213 Skin (dpeaa)DE-He213 Basement membrane (dpeaa)DE-He213 Type IV collagen (dpeaa)DE-He213 Connexin 43 (dpeaa)DE-He213 Epidermal thickness (dpeaa)DE-He213 |
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ddc 610 misc Diabetes misc Skin misc Basement membrane misc Type IV collagen misc Connexin 43 misc Epidermal thickness |
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ddc 610 misc Diabetes misc Skin misc Basement membrane misc Type IV collagen misc Connexin 43 misc Epidermal thickness |
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International journal of diabetes in developing countries |
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International journal of diabetes in developing countries |
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Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin |
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(DE-627)SPR031600271 (SPR)s13410-012-0070-6-e |
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Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin |
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Akarca, Saadet Özen |
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International journal of diabetes in developing countries |
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Akarca, Saadet Özen Yavaşoğlu, Altuğ Ayşegül, Uysal Fatih, Oltulu Yılmaz-Dilsiz, Özlem Timur, Köse Hüseyin, Aktuğ |
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investigation on the effects of experimental stz-induced diabetic rat model on basal membrane structures and gap junctions of skin |
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Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin |
| abstract |
Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. |
| abstractGer |
Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. |
| abstract_unstemmed |
Abstract This study was designed to determine the impairment of the skin structure in experimentally-induced diabetes with injection of streptozotocin (STZ). Experimental groups consisted of controls (group 1, N = 10) and diabetes groups (group 2, N = 10). Dorsal skin was removed for routine histological tissue procedures. Hematoxylene and Eosin (HE), Masson’s Trichrome and Periodic Acid Schiff (PAS) stainings, immunohistochemical connexin 43 (Cx43) and type IV collagen stainings were applied. Morphometry of epidermal thickness were also determined. Group 2 revealed decrease in epidermal thickness with disintegration of epithelium and decrease of dermal collagen fibers. Stratum spinosum were morphologically abnormal for group 2. Measurements of epidermal thickness revealed statistically significant decrease (P = 0.000). PAS staining for group 2 revealed disruption of the basement membrane. Epithelial scar formation, deterioration of transformation in the polyhedral cells, degradation of epidermis and decrease in PAS staining for vascular structures were observed, whereas the reticular dermis and hair follicles were normal. Collagen fiber density in group 2 were found to be prominently decreased in dermis with Masson’s Trichrome staining. Evident decrease in immunostaining of Cx43 and type IV collagen were also shown in diabetic group in comparison to the controls. In conclusion, diabetes not only induced impairment of the epidermal integrity and deterioration in the epidermis via loss of gap junctions (the most prominent cellular junctional complex), but also caused dramatically negative impact on the dermal collagen content, and integrity of the basement membrane. |
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| title_short |
Investigation on the effects of experimental STZ-induced diabetic rat model on basal membrane structures and gap junctions of skin |
| url |
https://dx.doi.org/10.1007/s13410-012-0070-6 |
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Yavaşoğlu, Altuğ Ayşegül, Uysal Fatih, Oltulu Yılmaz-Dilsiz, Özlem Timur, Köse Hüseyin, Aktuğ |
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Yavaşoğlu, Altuğ Ayşegül, Uysal Fatih, Oltulu Yılmaz-Dilsiz, Özlem Timur, Köse Hüseyin, Aktuğ |
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| score |
7.4015627 |