Anti-proliferative and genotoxic effects of arsenic and lead on human cells in vitro
Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arse...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ahmad, Aftab [verfasserIn] Shakoori, Abdul Rauf [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Toxicology and Environmental Health Sciences - Korean Society of Environmental Risk Assessment and Health Science, 2011, 6(2014), 3 vom: Sept., Seite 148-154 |
|---|---|
| Übergeordnetes Werk: |
volume:6 ; year:2014 ; number:3 ; month:09 ; pages:148-154 |
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| DOI / URN: |
10.1007/s13530-014-0199-4 |
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| 520 | |a Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. Overall, effects of arsenic exposure were more severe as compared to lead. | ||
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10.1007/s13530-014-0199-4 doi (DE-627)SPR031728014 (SPR)s13530-014-0199-4-e DE-627 ger DE-627 rakwb eng Ahmad, Aftab verfasserin aut Anti-proliferative and genotoxic effects of arsenic and lead on human cells in vitro 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. Overall, effects of arsenic exposure were more severe as compared to lead. HeLa cells (dpeaa)DE-He213 Genotoxic effect (dpeaa)DE-He213 Anti-proliferative activity (dpeaa)DE-He213 Comet assay (dpeaa)DE-He213 Heavy metals induced DNA damage (dpeaa)DE-He213 Shakoori, Abdul Rauf verfasserin aut Enthalten in Toxicology and Environmental Health Sciences Korean Society of Environmental Risk Assessment and Health Science, 2011 6(2014), 3 vom: Sept., Seite 148-154 (DE-627)SPR031726593 nnns volume:6 year:2014 number:3 month:09 pages:148-154 https://dx.doi.org/10.1007/s13530-014-0199-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 6 2014 3 09 148-154 |
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10.1007/s13530-014-0199-4 doi (DE-627)SPR031728014 (SPR)s13530-014-0199-4-e DE-627 ger DE-627 rakwb eng Ahmad, Aftab verfasserin aut Anti-proliferative and genotoxic effects of arsenic and lead on human cells in vitro 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. Overall, effects of arsenic exposure were more severe as compared to lead. HeLa cells (dpeaa)DE-He213 Genotoxic effect (dpeaa)DE-He213 Anti-proliferative activity (dpeaa)DE-He213 Comet assay (dpeaa)DE-He213 Heavy metals induced DNA damage (dpeaa)DE-He213 Shakoori, Abdul Rauf verfasserin aut Enthalten in Toxicology and Environmental Health Sciences Korean Society of Environmental Risk Assessment and Health Science, 2011 6(2014), 3 vom: Sept., Seite 148-154 (DE-627)SPR031726593 nnns volume:6 year:2014 number:3 month:09 pages:148-154 https://dx.doi.org/10.1007/s13530-014-0199-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER AR 6 2014 3 09 148-154 |
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Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. Overall, effects of arsenic exposure were more severe as compared to lead. |
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Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. Overall, effects of arsenic exposure were more severe as compared to lead. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR031728014</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20201125152601.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s13530-014-0199-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR031728014</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s13530-014-0199-4-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ahmad, Aftab</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Anti-proliferative and genotoxic effects of arsenic and lead on human cells in vitro</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Rapid industrialization and anthropogenic activities are main cause of environmental pollution, leading to an alarming situation. Both arsenic and lead are very dangerous to human health as exposure to these metal results in several abnormalities. In the present study, toxic effects of arsenic and lead were tested on HeLa cells. Cells were exposed to different concentrations of arsenic and lead and anti-proliferative response was tested using neutral red uptake assay. Comet assay was conducted to test the effect of arsenic and lead on DNA. Change in morphology and death of cells was observed after exposure to both metals. Comets were observed in the case of treated samples giving an indication of DNA damage. Ten different comet parameters were investigated for control and heavy metals treated samples. Present study clearly demonstrates that both arsenic and lead have anti-proliferative effect on HeLa cells and they also cause DNA damage. 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