The effect of purification of Ga-68-labeled exendin on in vivo distribution
Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this stu...
Ausführliche Beschreibung
Autor*in: |
Brom, Maarten [verfasserIn] Franssen, Gerben M. [verfasserIn] Joosten, Lieke [verfasserIn] Gotthardt, Martin [verfasserIn] Boerman, Otto C. [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2016 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
Enthalten in: EJNMMI Research - Berlin : Springer, 2011, 6(2016), 1 vom: 12. Aug. |
---|---|
Übergeordnetes Werk: |
volume:6 ; year:2016 ; number:1 ; day:12 ; month:08 |
Links: |
---|
DOI / URN: |
10.1186/s13550-016-0221-8 |
---|
Katalog-ID: |
SPR031781535 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | SPR031781535 | ||
003 | DE-627 | ||
005 | 20230519140534.0 | ||
007 | cr uuu---uuuuu | ||
008 | 201007s2016 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1186/s13550-016-0221-8 |2 doi | |
035 | |a (DE-627)SPR031781535 | ||
035 | |a (SPR)s13550-016-0221-8-e | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 610 |q ASE |
100 | 1 | |a Brom, Maarten |e verfasserin |4 aut | |
245 | 1 | 4 | |a The effect of purification of Ga-68-labeled exendin on in vivo distribution |
264 | 1 | |c 2016 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. | ||
650 | 4 | |a Ga |7 (dpeaa)DE-He213 | |
650 | 4 | |a Ga-hydroxide |7 (dpeaa)DE-He213 | |
650 | 4 | |a Purification |7 (dpeaa)DE-He213 | |
650 | 4 | |a Peptides |7 (dpeaa)DE-He213 | |
650 | 4 | |a Exendin |7 (dpeaa)DE-He213 | |
700 | 1 | |a Franssen, Gerben M. |e verfasserin |4 aut | |
700 | 1 | |a Joosten, Lieke |e verfasserin |4 aut | |
700 | 1 | |a Gotthardt, Martin |e verfasserin |4 aut | |
700 | 1 | |a Boerman, Otto C. |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t EJNMMI Research |d Berlin : Springer, 2011 |g 6(2016), 1 vom: 12. Aug. |w (DE-627)664970265 |w (DE-600)2619892-7 |x 2191-219X |7 nnns |
773 | 1 | 8 | |g volume:6 |g year:2016 |g number:1 |g day:12 |g month:08 |
856 | 4 | 0 | |u https://dx.doi.org/10.1186/s13550-016-0221-8 |z kostenfrei |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_SPRINGER | ||
912 | |a SSG-OLC-PHA | ||
912 | |a GBV_ILN_20 | ||
912 | |a GBV_ILN_22 | ||
912 | |a GBV_ILN_23 | ||
912 | |a GBV_ILN_24 | ||
912 | |a GBV_ILN_39 | ||
912 | |a GBV_ILN_40 | ||
912 | |a GBV_ILN_60 | ||
912 | |a GBV_ILN_62 | ||
912 | |a GBV_ILN_63 | ||
912 | |a GBV_ILN_65 | ||
912 | |a GBV_ILN_69 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_74 | ||
912 | |a GBV_ILN_95 | ||
912 | |a GBV_ILN_105 | ||
912 | |a GBV_ILN_110 | ||
912 | |a GBV_ILN_151 | ||
912 | |a GBV_ILN_161 | ||
912 | |a GBV_ILN_170 | ||
912 | |a GBV_ILN_206 | ||
912 | |a GBV_ILN_213 | ||
912 | |a GBV_ILN_230 | ||
912 | |a GBV_ILN_285 | ||
912 | |a GBV_ILN_293 | ||
912 | |a GBV_ILN_602 | ||
912 | |a GBV_ILN_2005 | ||
912 | |a GBV_ILN_2009 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2055 | ||
912 | |a GBV_ILN_2111 | ||
912 | |a GBV_ILN_4012 | ||
912 | |a GBV_ILN_4037 | ||
912 | |a GBV_ILN_4112 | ||
912 | |a GBV_ILN_4125 | ||
912 | |a GBV_ILN_4126 | ||
912 | |a GBV_ILN_4249 | ||
912 | |a GBV_ILN_4305 | ||
912 | |a GBV_ILN_4306 | ||
912 | |a GBV_ILN_4307 | ||
912 | |a GBV_ILN_4313 | ||
912 | |a GBV_ILN_4322 | ||
912 | |a GBV_ILN_4323 | ||
912 | |a GBV_ILN_4324 | ||
912 | |a GBV_ILN_4325 | ||
912 | |a GBV_ILN_4338 | ||
912 | |a GBV_ILN_4367 | ||
912 | |a GBV_ILN_4700 | ||
951 | |a AR | ||
952 | |d 6 |j 2016 |e 1 |b 12 |c 08 |
author_variant |
m b mb g m f gm gmf l j lj m g mg o c b oc ocb |
---|---|
matchkey_str |
article:2191219X:2016----::hefcoprfctoog6lbldxnio |
hierarchy_sort_str |
2016 |
publishDate |
2016 |
allfields |
10.1186/s13550-016-0221-8 doi (DE-627)SPR031781535 (SPR)s13550-016-0221-8-e DE-627 ger DE-627 rakwb eng 610 ASE Brom, Maarten verfasserin aut The effect of purification of Ga-68-labeled exendin on in vivo distribution 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 Franssen, Gerben M. verfasserin aut Joosten, Lieke verfasserin aut Gotthardt, Martin verfasserin aut Boerman, Otto C. verfasserin aut Enthalten in EJNMMI Research Berlin : Springer, 2011 6(2016), 1 vom: 12. Aug. (DE-627)664970265 (DE-600)2619892-7 2191-219X nnns volume:6 year:2016 number:1 day:12 month:08 https://dx.doi.org/10.1186/s13550-016-0221-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016 1 12 08 |
spelling |
10.1186/s13550-016-0221-8 doi (DE-627)SPR031781535 (SPR)s13550-016-0221-8-e DE-627 ger DE-627 rakwb eng 610 ASE Brom, Maarten verfasserin aut The effect of purification of Ga-68-labeled exendin on in vivo distribution 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 Franssen, Gerben M. verfasserin aut Joosten, Lieke verfasserin aut Gotthardt, Martin verfasserin aut Boerman, Otto C. verfasserin aut Enthalten in EJNMMI Research Berlin : Springer, 2011 6(2016), 1 vom: 12. Aug. (DE-627)664970265 (DE-600)2619892-7 2191-219X nnns volume:6 year:2016 number:1 day:12 month:08 https://dx.doi.org/10.1186/s13550-016-0221-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016 1 12 08 |
allfields_unstemmed |
10.1186/s13550-016-0221-8 doi (DE-627)SPR031781535 (SPR)s13550-016-0221-8-e DE-627 ger DE-627 rakwb eng 610 ASE Brom, Maarten verfasserin aut The effect of purification of Ga-68-labeled exendin on in vivo distribution 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 Franssen, Gerben M. verfasserin aut Joosten, Lieke verfasserin aut Gotthardt, Martin verfasserin aut Boerman, Otto C. verfasserin aut Enthalten in EJNMMI Research Berlin : Springer, 2011 6(2016), 1 vom: 12. Aug. (DE-627)664970265 (DE-600)2619892-7 2191-219X nnns volume:6 year:2016 number:1 day:12 month:08 https://dx.doi.org/10.1186/s13550-016-0221-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016 1 12 08 |
allfieldsGer |
10.1186/s13550-016-0221-8 doi (DE-627)SPR031781535 (SPR)s13550-016-0221-8-e DE-627 ger DE-627 rakwb eng 610 ASE Brom, Maarten verfasserin aut The effect of purification of Ga-68-labeled exendin on in vivo distribution 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 Franssen, Gerben M. verfasserin aut Joosten, Lieke verfasserin aut Gotthardt, Martin verfasserin aut Boerman, Otto C. verfasserin aut Enthalten in EJNMMI Research Berlin : Springer, 2011 6(2016), 1 vom: 12. Aug. (DE-627)664970265 (DE-600)2619892-7 2191-219X nnns volume:6 year:2016 number:1 day:12 month:08 https://dx.doi.org/10.1186/s13550-016-0221-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016 1 12 08 |
allfieldsSound |
10.1186/s13550-016-0221-8 doi (DE-627)SPR031781535 (SPR)s13550-016-0221-8-e DE-627 ger DE-627 rakwb eng 610 ASE Brom, Maarten verfasserin aut The effect of purification of Ga-68-labeled exendin on in vivo distribution 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 Franssen, Gerben M. verfasserin aut Joosten, Lieke verfasserin aut Gotthardt, Martin verfasserin aut Boerman, Otto C. verfasserin aut Enthalten in EJNMMI Research Berlin : Springer, 2011 6(2016), 1 vom: 12. Aug. (DE-627)664970265 (DE-600)2619892-7 2191-219X nnns volume:6 year:2016 number:1 day:12 month:08 https://dx.doi.org/10.1186/s13550-016-0221-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016 1 12 08 |
language |
English |
source |
Enthalten in EJNMMI Research 6(2016), 1 vom: 12. Aug. volume:6 year:2016 number:1 day:12 month:08 |
sourceStr |
Enthalten in EJNMMI Research 6(2016), 1 vom: 12. Aug. volume:6 year:2016 number:1 day:12 month:08 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
Ga Ga-hydroxide Purification Peptides Exendin |
dewey-raw |
610 |
isfreeaccess_bool |
true |
container_title |
EJNMMI Research |
authorswithroles_txt_mv |
Brom, Maarten @@aut@@ Franssen, Gerben M. @@aut@@ Joosten, Lieke @@aut@@ Gotthardt, Martin @@aut@@ Boerman, Otto C. @@aut@@ |
publishDateDaySort_date |
2016-08-12T00:00:00Z |
hierarchy_top_id |
664970265 |
dewey-sort |
3610 |
id |
SPR031781535 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR031781535</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519140534.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s13550-016-0221-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR031781535</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s13550-016-0221-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Brom, Maarten</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The effect of purification of Ga-68-labeled exendin on in vivo distribution</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ga</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ga-hydroxide</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Purification</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Peptides</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Exendin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Franssen, Gerben M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Joosten, Lieke</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gotthardt, Martin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Boerman, Otto C.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">EJNMMI Research</subfield><subfield code="d">Berlin : Springer, 2011</subfield><subfield code="g">6(2016), 1 vom: 12. Aug.</subfield><subfield code="w">(DE-627)664970265</subfield><subfield code="w">(DE-600)2619892-7</subfield><subfield code="x">2191-219X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:6</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:1</subfield><subfield code="g">day:12</subfield><subfield code="g">month:08</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1186/s13550-016-0221-8</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">6</subfield><subfield code="j">2016</subfield><subfield code="e">1</subfield><subfield code="b">12</subfield><subfield code="c">08</subfield></datafield></record></collection>
|
author |
Brom, Maarten |
spellingShingle |
Brom, Maarten ddc 610 misc Ga misc Ga-hydroxide misc Purification misc Peptides misc Exendin The effect of purification of Ga-68-labeled exendin on in vivo distribution |
authorStr |
Brom, Maarten |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)664970265 |
format |
electronic Article |
dewey-ones |
610 - Medicine & health |
delete_txt_mv |
keep |
author_role |
aut aut aut aut aut |
collection |
springer |
remote_str |
true |
illustrated |
Not Illustrated |
issn |
2191-219X |
topic_title |
610 ASE The effect of purification of Ga-68-labeled exendin on in vivo distribution Ga (dpeaa)DE-He213 Ga-hydroxide (dpeaa)DE-He213 Purification (dpeaa)DE-He213 Peptides (dpeaa)DE-He213 Exendin (dpeaa)DE-He213 |
topic |
ddc 610 misc Ga misc Ga-hydroxide misc Purification misc Peptides misc Exendin |
topic_unstemmed |
ddc 610 misc Ga misc Ga-hydroxide misc Purification misc Peptides misc Exendin |
topic_browse |
ddc 610 misc Ga misc Ga-hydroxide misc Purification misc Peptides misc Exendin |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
cr |
hierarchy_parent_title |
EJNMMI Research |
hierarchy_parent_id |
664970265 |
dewey-tens |
610 - Medicine & health |
hierarchy_top_title |
EJNMMI Research |
isfreeaccess_txt |
true |
familylinks_str_mv |
(DE-627)664970265 (DE-600)2619892-7 |
title |
The effect of purification of Ga-68-labeled exendin on in vivo distribution |
ctrlnum |
(DE-627)SPR031781535 (SPR)s13550-016-0221-8-e |
title_full |
The effect of purification of Ga-68-labeled exendin on in vivo distribution |
author_sort |
Brom, Maarten |
journal |
EJNMMI Research |
journalStr |
EJNMMI Research |
lang_code |
eng |
isOA_bool |
true |
dewey-hundreds |
600 - Technology |
recordtype |
marc |
publishDateSort |
2016 |
contenttype_str_mv |
txt |
author_browse |
Brom, Maarten Franssen, Gerben M. Joosten, Lieke Gotthardt, Martin Boerman, Otto C. |
container_volume |
6 |
class |
610 ASE |
format_se |
Elektronische Aufsätze |
author-letter |
Brom, Maarten |
doi_str_mv |
10.1186/s13550-016-0221-8 |
dewey-full |
610 |
author2-role |
verfasserin |
title_sort |
effect of purification of ga-68-labeled exendin on in vivo distribution |
title_auth |
The effect of purification of Ga-68-labeled exendin on in vivo distribution |
abstract |
Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. |
abstractGer |
Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. |
abstract_unstemmed |
Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 |
container_issue |
1 |
title_short |
The effect of purification of Ga-68-labeled exendin on in vivo distribution |
url |
https://dx.doi.org/10.1186/s13550-016-0221-8 |
remote_bool |
true |
author2 |
Franssen, Gerben M. Joosten, Lieke Gotthardt, Martin Boerman, Otto C. |
author2Str |
Franssen, Gerben M. Joosten, Lieke Gotthardt, Martin Boerman, Otto C. |
ppnlink |
664970265 |
mediatype_str_mv |
c |
isOA_txt |
true |
hochschulschrift_bool |
false |
doi_str |
10.1186/s13550-016-0221-8 |
up_date |
2024-07-04T01:12:47.034Z |
_version_ |
1803608986261389312 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR031781535</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519140534.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s13550-016-0221-8</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR031781535</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s13550-016-0221-8-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Brom, Maarten</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="4"><subfield code="a">The effect of purification of Ga-68-labeled exendin on in vivo distribution</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of 68Ga-colloid may occur. Upon i.v. injection, 68Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we studied the effect of the purification method on the in vivo characteristics of 68Ga-labeled DOTA-exendin-3. DOTA-exendin-3 was labeled with 68Ga, and the colloid content was measured by TLC on silica gel ITLC with two mobile phases. The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a $ C_{8} $ column or by solid phase extraction (SPE) on an HLB cartridge. The in vivo characteristics of the preparations were determined in BALB/c nude mice, and PET images were acquired 1 h p.i. using a microPET scanner. In these studies, unpurified 68Ga-DOTA-exendin-3 and 111In-DOTA-exendin-3 were used as a reference. Results The colloid content of 111In-DOTA-exendin-3 and unpurified, gel filtration, RP-HPLC- and SPE-purified 68Ga-DOTA exendin-3 was <3, 7, 9, <3, and <3 %, respectively. Unpurified 68Ga-DOTA exendin-3 showed high liver and spleen uptake. Gel filtration partly removed 68Ga-colloid from the preparation, resulting in moderate liver and spleen SPE-purified 68Ga-DOTA exendin-3 showed very low liver and spleen uptake, that was similar to that of RP-HPLC purified 68Ga-DOTA exendin-3. Conclusions We showed that the colloid content can be measured by TLC and that solid phase extraction and HPLC completely remove 68Ga-colloid from 68Ga-labeled tracer preparations, resulting in very low liver and spleen uptake. This study clearly shows the importance of removal of 68Ga-colloid from preparations.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ga</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ga-hydroxide</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Purification</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Peptides</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Exendin</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Franssen, Gerben M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Joosten, Lieke</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gotthardt, Martin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Boerman, Otto C.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">EJNMMI Research</subfield><subfield code="d">Berlin : Springer, 2011</subfield><subfield code="g">6(2016), 1 vom: 12. Aug.</subfield><subfield code="w">(DE-627)664970265</subfield><subfield code="w">(DE-600)2619892-7</subfield><subfield code="x">2191-219X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:6</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:1</subfield><subfield code="g">day:12</subfield><subfield code="g">month:08</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1186/s13550-016-0221-8</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">6</subfield><subfield code="j">2016</subfield><subfield code="e">1</subfield><subfield code="b">12</subfield><subfield code="c">08</subfield></datafield></record></collection>
|
score |
7.4017067 |