In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit
Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkab...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Loc, Nguyen Hoang [verfasserIn] Song, Nguyen Van [verfasserIn] Long, Dang Thanh [verfasserIn] Kim, Tae-Geum [verfasserIn] Yang, Moon-Sik [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of plant biochemistry and biotechnology - Berlin : Springer, 1992, 24(2013), 2 vom: 11. Nov., Seite 129-134 |
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| Übergeordnetes Werk: |
volume:24 ; year:2013 ; number:2 ; day:11 ; month:11 ; pages:129-134 |
| Links: |
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| DOI / URN: |
10.1007/s13562-013-0244-4 |
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| Katalog-ID: |
SPR031802974 |
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| 100 | 1 | |a Loc, Nguyen Hoang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
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| 520 | |a Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. | ||
| 650 | 4 | |a Growth |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a LTB |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Physiological and biochemical characteristics |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Song, Nguyen Van |e verfasserin |4 aut | |
| 700 | 1 | |a Long, Dang Thanh |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Tae-Geum |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Moon-Sik |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of plant biochemistry and biotechnology |d Berlin : Springer, 1992 |g 24(2013), 2 vom: 11. Nov., Seite 129-134 |w (DE-627)501417192 |w (DE-600)2206337-7 |x 0974-1275 |7 nnns |
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| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
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| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
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2013 |
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10.1007/s13562-013-0244-4 doi (DE-627)SPR031802974 (SPR)s13562-013-0244-4-e DE-627 ger DE-627 rakwb eng 540 570 ASE Loc, Nguyen Hoang verfasserin aut In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 Song, Nguyen Van verfasserin aut Long, Dang Thanh verfasserin aut Kim, Tae-Geum verfasserin aut Yang, Moon-Sik verfasserin aut Enthalten in Journal of plant biochemistry and biotechnology Berlin : Springer, 1992 24(2013), 2 vom: 11. Nov., Seite 129-134 (DE-627)501417192 (DE-600)2206337-7 0974-1275 nnns volume:24 year:2013 number:2 day:11 month:11 pages:129-134 https://dx.doi.org/10.1007/s13562-013-0244-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2013 2 11 11 129-134 |
| spelling |
10.1007/s13562-013-0244-4 doi (DE-627)SPR031802974 (SPR)s13562-013-0244-4-e DE-627 ger DE-627 rakwb eng 540 570 ASE Loc, Nguyen Hoang verfasserin aut In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 Song, Nguyen Van verfasserin aut Long, Dang Thanh verfasserin aut Kim, Tae-Geum verfasserin aut Yang, Moon-Sik verfasserin aut Enthalten in Journal of plant biochemistry and biotechnology Berlin : Springer, 1992 24(2013), 2 vom: 11. Nov., Seite 129-134 (DE-627)501417192 (DE-600)2206337-7 0974-1275 nnns volume:24 year:2013 number:2 day:11 month:11 pages:129-134 https://dx.doi.org/10.1007/s13562-013-0244-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2013 2 11 11 129-134 |
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10.1007/s13562-013-0244-4 doi (DE-627)SPR031802974 (SPR)s13562-013-0244-4-e DE-627 ger DE-627 rakwb eng 540 570 ASE Loc, Nguyen Hoang verfasserin aut In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 Song, Nguyen Van verfasserin aut Long, Dang Thanh verfasserin aut Kim, Tae-Geum verfasserin aut Yang, Moon-Sik verfasserin aut Enthalten in Journal of plant biochemistry and biotechnology Berlin : Springer, 1992 24(2013), 2 vom: 11. Nov., Seite 129-134 (DE-627)501417192 (DE-600)2206337-7 0974-1275 nnns volume:24 year:2013 number:2 day:11 month:11 pages:129-134 https://dx.doi.org/10.1007/s13562-013-0244-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2013 2 11 11 129-134 |
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10.1007/s13562-013-0244-4 doi (DE-627)SPR031802974 (SPR)s13562-013-0244-4-e DE-627 ger DE-627 rakwb eng 540 570 ASE Loc, Nguyen Hoang verfasserin aut In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 Song, Nguyen Van verfasserin aut Long, Dang Thanh verfasserin aut Kim, Tae-Geum verfasserin aut Yang, Moon-Sik verfasserin aut Enthalten in Journal of plant biochemistry and biotechnology Berlin : Springer, 1992 24(2013), 2 vom: 11. Nov., Seite 129-134 (DE-627)501417192 (DE-600)2206337-7 0974-1275 nnns volume:24 year:2013 number:2 day:11 month:11 pages:129-134 https://dx.doi.org/10.1007/s13562-013-0244-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2013 2 11 11 129-134 |
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10.1007/s13562-013-0244-4 doi (DE-627)SPR031802974 (SPR)s13562-013-0244-4-e DE-627 ger DE-627 rakwb eng 540 570 ASE Loc, Nguyen Hoang verfasserin aut In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit 2013 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 Song, Nguyen Van verfasserin aut Long, Dang Thanh verfasserin aut Kim, Tae-Geum verfasserin aut Yang, Moon-Sik verfasserin aut Enthalten in Journal of plant biochemistry and biotechnology Berlin : Springer, 1992 24(2013), 2 vom: 11. Nov., Seite 129-134 (DE-627)501417192 (DE-600)2206337-7 0974-1275 nnns volume:24 year:2013 number:2 day:11 month:11 pages:129-134 https://dx.doi.org/10.1007/s13562-013-0244-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 24 2013 2 11 11 129-134 |
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Enthalten in Journal of plant biochemistry and biotechnology 24(2013), 2 vom: 11. Nov., Seite 129-134 volume:24 year:2013 number:2 day:11 month:11 pages:129-134 |
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Loc, Nguyen Hoang @@aut@@ Song, Nguyen Van @@aut@@ Long, Dang Thanh @@aut@@ Kim, Tae-Geum @@aut@@ Yang, Moon-Sik @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR031802974</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519192518.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2013 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s13562-013-0244-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR031802974</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s13562-013-0244-4-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="a">570</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Loc, Nguyen Hoang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2013</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. 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Loc, Nguyen Hoang |
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Loc, Nguyen Hoang ddc 540 misc Growth misc LTB misc Physiological and biochemical characteristics In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
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540 570 ASE In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit Growth (dpeaa)DE-He213 LTB (dpeaa)DE-He213 Physiological and biochemical characteristics (dpeaa)DE-He213 |
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ddc 540 misc Growth misc LTB misc Physiological and biochemical characteristics |
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In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
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In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
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Loc, Nguyen Hoang |
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Journal of plant biochemistry and biotechnology |
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Loc, Nguyen Hoang Song, Nguyen Van Long, Dang Thanh Kim, Tae-Geum Yang, Moon-Sik |
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in vivo evaluation of transgenic watercress containing gene encoding escherichia coli heat-labile toxin b subunit |
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In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
| abstract |
Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. |
| abstractGer |
Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. |
| abstract_unstemmed |
Abstract In this work, the characterizations of the LTB (Escherichia coli heat-labile toxin B subunit) transgenic watercresses through Agrobacterium tumefaciens-mediated transformation (A1 and A3-A5) and by using biolistic method (B1 and B4) were investigated. Generally, their growth is not remarkably different from the wild-type. Their physiological and biochemical characteristics are relatively different in which plant A1 has highest values such as pigment (0.92 mg $ g^{−1} $), photosynthetic rate (23.39 $ mgCO_{2} $ [$ dm^{2} $]−1 $ h^{−1} $), dry matter (7.42 %), vitamin C (0.34 mg $ g^{−1} $), calcium (0.83 mg $ g^{−1} $), and potassium (2.47 mg $ g^{−1} $). The dry matter and calcium of all the transgenic watercresses and the wild-type are the same content. Southern blot hybridization showed the transgenic plants contain 1–2 copies in the genome. LTB protein strongly expresses in all the transgenic plants with contents from 1.16 to 1.46 % of total soluble protein. The GM1-ELISA binding assay indicated that plant-derived LTB protein bound to GM1-ganglioside receptors. |
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2 |
| title_short |
In vivo evaluation of transgenic watercress containing gene encoding Escherichia coli heat-labile toxin B subunit |
| url |
https://dx.doi.org/10.1007/s13562-013-0244-4 |
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| author2 |
Song, Nguyen Van Long, Dang Thanh Kim, Tae-Geum Yang, Moon-Sik |
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Song, Nguyen Van Long, Dang Thanh Kim, Tae-Geum Yang, Moon-Sik |
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| doi_str |
10.1007/s13562-013-0244-4 |
| up_date |
2024-07-04T01:18:24.836Z |
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| score |
7.39968 |