Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions

Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Welles, L. [verfasserIn] Lopez-Vazquez, C. M. [verfasserIn] Hooijmans, C. M. [verfasserIn] van Loosdrecht, M. C. M. [verfasserIn] Brdjanovic, D. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Polyphosphate-accumulating organisms (PAO) Glycogen-accumulating organisms (GAO) Enhanced biological phosphate removal (EBPR) Polyphosphate content Microbial ecology Niche differentiation ‘ Accumulibacter phosphatis’ Competibacter phosphatis’

Übergeordnetes Werk:	Enthalten in: AMB express - Heidelberg : Springer, 2011, 6(2016), 1 vom: 04. Juli
Übergeordnetes Werk:	volume:6 ; year:2016 ; number:1 ; day:04 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s13568-016-0214-z

Katalog-ID:	SPR031829937

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520			\|a Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available.
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allfields	10.1186/s13568-016-0214-z doi (DE-627)SPR031829937 (SPR)s13568-016-0214-z-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl Welles, L. verfasserin aut Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213 Lopez-Vazquez, C. M. verfasserin aut Hooijmans, C. M. verfasserin aut van Loosdrecht, M. C. M. verfasserin aut Brdjanovic, D. verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 6(2016), 1 vom: 04. Juli (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:6 year:2016 number:1 day:04 month:07 https://dx.doi.org/10.1186/s13568-016-0214-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 6 2016 1 04 07
spelling	10.1186/s13568-016-0214-z doi (DE-627)SPR031829937 (SPR)s13568-016-0214-z-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl Welles, L. verfasserin aut Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213 Lopez-Vazquez, C. M. verfasserin aut Hooijmans, C. M. verfasserin aut van Loosdrecht, M. C. M. verfasserin aut Brdjanovic, D. verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 6(2016), 1 vom: 04. Juli (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:6 year:2016 number:1 day:04 month:07 https://dx.doi.org/10.1186/s13568-016-0214-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 6 2016 1 04 07
allfields_unstemmed	10.1186/s13568-016-0214-z doi (DE-627)SPR031829937 (SPR)s13568-016-0214-z-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl Welles, L. verfasserin aut Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213 Lopez-Vazquez, C. M. verfasserin aut Hooijmans, C. M. verfasserin aut van Loosdrecht, M. C. M. verfasserin aut Brdjanovic, D. verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 6(2016), 1 vom: 04. Juli (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:6 year:2016 number:1 day:04 month:07 https://dx.doi.org/10.1186/s13568-016-0214-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 6 2016 1 04 07
allfieldsGer	10.1186/s13568-016-0214-z doi (DE-627)SPR031829937 (SPR)s13568-016-0214-z-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl Welles, L. verfasserin aut Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213 Lopez-Vazquez, C. M. verfasserin aut Hooijmans, C. M. verfasserin aut van Loosdrecht, M. C. M. verfasserin aut Brdjanovic, D. verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 6(2016), 1 vom: 04. Juli (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:6 year:2016 number:1 day:04 month:07 https://dx.doi.org/10.1186/s13568-016-0214-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 6 2016 1 04 07
allfieldsSound	10.1186/s13568-016-0214-z doi (DE-627)SPR031829937 (SPR)s13568-016-0214-z-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl Welles, L. verfasserin aut Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available. Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213 Lopez-Vazquez, C. M. verfasserin aut Hooijmans, C. M. verfasserin aut van Loosdrecht, M. C. M. verfasserin aut Brdjanovic, D. verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 6(2016), 1 vom: 04. Juli (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:6 year:2016 number:1 day:04 month:07 https://dx.doi.org/10.1186/s13568-016-0214-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 6 2016 1 04 07
language	English
source	Enthalten in AMB express 6(2016), 1 vom: 04. Juli volume:6 year:2016 number:1 day:04 month:07
sourceStr	Enthalten in AMB express 6(2016), 1 vom: 04. Juli volume:6 year:2016 number:1 day:04 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Polyphosphate-accumulating organisms (PAO) Glycogen-accumulating organisms (GAO) Enhanced biological phosphate removal (EBPR) Polyphosphate content Microbial ecology Niche differentiation ‘ Accumulibacter phosphatis’ Competibacter phosphatis’
dewey-raw	570
isfreeaccess_bool	true
container_title	AMB express
authorswithroles_txt_mv	Welles, L. @@aut@@ Lopez-Vazquez, C. M. @@aut@@ Hooijmans, C. M. @@aut@@ van Loosdrecht, M. C. M. @@aut@@ Brdjanovic, D. @@aut@@
publishDateDaySort_date	2016-07-04T00:00:00Z
hierarchy_top_id	665672926
dewey-sort	3570
id	SPR031829937
language_de	englisch
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author	Welles, L.
spellingShingle	Welles, L. ddc 570 bkl 58.30 misc Polyphosphate-accumulating organisms (PAO) misc Glycogen-accumulating organisms (GAO) misc Enhanced biological phosphate removal (EBPR) misc Polyphosphate content misc Microbial ecology misc Niche differentiation misc ‘ misc Accumulibacter phosphatis’ misc Competibacter phosphatis’ Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
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topic_title	570 ASE 58.30 bkl Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions Polyphosphate-accumulating organisms (PAO) (dpeaa)DE-He213 Glycogen-accumulating organisms (GAO) (dpeaa)DE-He213 Enhanced biological phosphate removal (EBPR) (dpeaa)DE-He213 Polyphosphate content (dpeaa)DE-He213 Microbial ecology (dpeaa)DE-He213 Niche differentiation (dpeaa)DE-He213 ‘ (dpeaa)DE-He213 Accumulibacter phosphatis’ (dpeaa)DE-He213 Competibacter phosphatis’ (dpeaa)DE-He213
topic	ddc 570 bkl 58.30 misc Polyphosphate-accumulating organisms (PAO) misc Glycogen-accumulating organisms (GAO) misc Enhanced biological phosphate removal (EBPR) misc Polyphosphate content misc Microbial ecology misc Niche differentiation misc ‘ misc Accumulibacter phosphatis’ misc Competibacter phosphatis’
topic_unstemmed	ddc 570 bkl 58.30 misc Polyphosphate-accumulating organisms (PAO) misc Glycogen-accumulating organisms (GAO) misc Enhanced biological phosphate removal (EBPR) misc Polyphosphate content misc Microbial ecology misc Niche differentiation misc ‘ misc Accumulibacter phosphatis’ misc Competibacter phosphatis’
topic_browse	ddc 570 bkl 58.30 misc Polyphosphate-accumulating organisms (PAO) misc Glycogen-accumulating organisms (GAO) misc Enhanced biological phosphate removal (EBPR) misc Polyphosphate content misc Microbial ecology misc Niche differentiation misc ‘ misc Accumulibacter phosphatis’ misc Competibacter phosphatis’
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title	Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
ctrlnum	(DE-627)SPR031829937 (SPR)s13568-016-0214-z-e
title_full	Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
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author_browse	Welles, L. Lopez-Vazquez, C. M. Hooijmans, C. M. van Loosdrecht, M. C. M. Brdjanovic, D.
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title_sort	prevalence of ‘candidatus accumulibacter phosphatis’ type ii under phosphate limiting conditions
title_auth	Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
abstract	Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available.
abstractGer	Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available.
abstract_unstemmed	Abstract P-limitation in enhanced biological phosphorus removal (EBPR) systems fed with acetate, has generally been considered as a condition leading to enrichment of organisms of the genotype’ Candidatus Competibacter phosphatis’ expressing the glycogen-accumulating organisms (GAO) phenotype. Recent studies have demonstrated in short-term experiments that organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ clade I and II, known to express the polyphosphate-accumulating organisms (PAO) phenotype can switch to the GAO phenotype when poly-P is absent, but are performing the HAc-uptake at lower kinetic rates, where clade I showed the lowest rates. The objective of this study was to verify whether organisms of the genotype ‘Candidatus Accumulibacter phosphatis’ can also be enriched under P-limiting conditions while expressing a GAO phenotype and more specifically to see which specific clade prevails. A sequencing batch reactor was inoculated with activated sludge to enrich an EBPR culture for a cultivation period of 128 days (16 times the solids retention time) under P-limiting conditions. A mixed culture was obtained comprising of 49 % ‘Candidatus Accumulibacter phosphatis’ clade II and 46 % ‘Candidatus Competibacter phosphatis’. The culture performed a full GAO metabolism for anaerobic HAc-uptake, but was still able to switch to a PAO metabolism, taking up excessive amounts of phosphate during the aerobic phase when it became available in the influent. These findings show that P-limitation, often used as strategy for enrichment of ‘Candidatus Competibacter phosphatis’, does not always lead to enrichment of only ‘Candidatus Competibacter phosphatis’. Furthermore, it demonstrates that ‘Candidatus Accumulibacter phosphatis’ are able to proliferate in activated sludge systems for periods of up to 128 days or longer when the influent phosphate concentrations are just enough for assimilation purposes and no poly-P is formed. The ‘Candidatus Accumulibacter phosphatis’ retain the ability to switch to the PAO phenotype, taking up phosphate from the influent as soon as it becomes available.
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container_issue	1
title_short	Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions
url	https://dx.doi.org/10.1186/s13568-016-0214-z
remote_bool	true
author2	Lopez-Vazquez, C. M. Hooijmans, C. M. van Loosdrecht, M. C. M. Brdjanovic, D.
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up_date	2024-07-04T01:25:44.529Z
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Prevalence of ‘Candidatus Accumulibacter phosphatis’ type II under phosphate limiting conditions

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