A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production
Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23...
Ausführliche Beschreibung
Autor*in: |
El-Rotail, Ashraf A. M. M. [verfasserIn] Zhang, Liang [verfasserIn] Li, Youran [verfasserIn] Liu, Shuang Ping [verfasserIn] Shi, Gui Yang [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2017 |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: AMB express - Heidelberg : Springer, 2011, 7(2017), 1 vom: 02. Juni |
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Übergeordnetes Werk: |
volume:7 ; year:2017 ; number:1 ; day:02 ; month:06 |
Links: |
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DOI / URN: |
10.1186/s13568-017-0400-7 |
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Katalog-ID: |
SPR031831974 |
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520 | |a Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. | ||
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700 | 1 | |a Shi, Gui Yang |e verfasserin |4 aut | |
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10.1186/s13568-017-0400-7 doi (DE-627)SPR031831974 (SPR)s13568-017-0400-7-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl El-Rotail, Ashraf A. M. M. verfasserin aut A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 Zhang, Liang verfasserin aut Li, Youran verfasserin aut Liu, Shuang Ping verfasserin aut Shi, Gui Yang verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 7(2017), 1 vom: 02. Juni (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:7 year:2017 number:1 day:02 month:06 https://dx.doi.org/10.1186/s13568-017-0400-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 7 2017 1 02 06 |
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10.1186/s13568-017-0400-7 doi (DE-627)SPR031831974 (SPR)s13568-017-0400-7-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl El-Rotail, Ashraf A. M. M. verfasserin aut A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 Zhang, Liang verfasserin aut Li, Youran verfasserin aut Liu, Shuang Ping verfasserin aut Shi, Gui Yang verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 7(2017), 1 vom: 02. Juni (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:7 year:2017 number:1 day:02 month:06 https://dx.doi.org/10.1186/s13568-017-0400-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 7 2017 1 02 06 |
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10.1186/s13568-017-0400-7 doi (DE-627)SPR031831974 (SPR)s13568-017-0400-7-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl El-Rotail, Ashraf A. M. M. verfasserin aut A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 Zhang, Liang verfasserin aut Li, Youran verfasserin aut Liu, Shuang Ping verfasserin aut Shi, Gui Yang verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 7(2017), 1 vom: 02. Juni (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:7 year:2017 number:1 day:02 month:06 https://dx.doi.org/10.1186/s13568-017-0400-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 7 2017 1 02 06 |
allfieldsGer |
10.1186/s13568-017-0400-7 doi (DE-627)SPR031831974 (SPR)s13568-017-0400-7-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl El-Rotail, Ashraf A. M. M. verfasserin aut A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 Zhang, Liang verfasserin aut Li, Youran verfasserin aut Liu, Shuang Ping verfasserin aut Shi, Gui Yang verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 7(2017), 1 vom: 02. Juni (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:7 year:2017 number:1 day:02 month:06 https://dx.doi.org/10.1186/s13568-017-0400-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 7 2017 1 02 06 |
allfieldsSound |
10.1186/s13568-017-0400-7 doi (DE-627)SPR031831974 (SPR)s13568-017-0400-7-e DE-627 ger DE-627 rakwb eng 570 ASE 58.30 bkl El-Rotail, Ashraf A. M. M. verfasserin aut A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 Zhang, Liang verfasserin aut Li, Youran verfasserin aut Liu, Shuang Ping verfasserin aut Shi, Gui Yang verfasserin aut Enthalten in AMB express Heidelberg : Springer, 2011 7(2017), 1 vom: 02. Juni (DE-627)665672926 (DE-600)2621432-5 2191-0855 nnns volume:7 year:2017 number:1 day:02 month:06 https://dx.doi.org/10.1186/s13568-017-0400-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE AR 7 2017 1 02 06 |
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El-Rotail, Ashraf A. M. M. |
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El-Rotail, Ashraf A. M. M. ddc 570 bkl 58.30 misc Bioethanol misc Error-prone PCR misc Ethanol production misc Ethanol tolerance misc Global transcription machinery engineering A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production |
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570 ASE 58.30 bkl A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production Bioethanol (dpeaa)DE-He213 Error-prone PCR (dpeaa)DE-He213 Ethanol production (dpeaa)DE-He213 Ethanol tolerance (dpeaa)DE-He213 Global transcription machinery engineering (dpeaa)DE-He213 |
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novel constructed spt15 mutagenesis library of saccharomyces cerevisiae by using gtme technique for enhanced ethanol production |
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A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production |
abstract |
Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. |
abstractGer |
Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. |
abstract_unstemmed |
Abstract During the last few years, the global transcription machinery engineering (gTME) technique has gained more attention as an effective approach for the construction of novel mutants. Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain. |
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A novel constructed SPT15 mutagenesis library of Saccharomyces cerevisiae by using gTME technique for enhanced ethanol production |
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Genetic strategies (molecular biology methods) were utilized to get mutational for both genes (SPT15 and TAF23) basically existed in the Saccharomyces cerevisiae genome via screening the gTME approach in order to obtain a new mutant S. cerevisiae diploid strain. The vector pYX212 was utilized to transform these genes into the control diploid strain S. cerevisiae through the process of mating between haploids control strains S. cerevisiae (MAT-a [CICC 1374]) and (MAT-α [CICC 31144]), by using the oligonucleotide primers SPT15-EcoRI-FW/SPT15-SalI-RV and TAF23-SalI-FW/TAF23-NheI-RV, respectively. The resultant mutants were examined for a series of stability tests. This study showed how strong the effect of using strategic gTME with the importance of the modification we conducted in Error Prone PCR protocol by increasing $ MnCl_{2} $ concentration instead of $ MgCl_{2} $. 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