Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies
Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. Whi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Boyle, Natalie K. [verfasserIn] Machtley, Scott A. [verfasserIn] Hagler, James R. [verfasserIn] Pitts-Singer, Theresa L. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Apidologie - Paris : Springer, 1970, 49(2018), 3 vom: 28. Feb., Seite 378-385 |
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| Übergeordnetes Werk: |
volume:49 ; year:2018 ; number:3 ; day:28 ; month:02 ; pages:378-385 |
| Links: |
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| DOI / URN: |
10.1007/s13592-018-0564-4 |
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| Katalog-ID: |
SPR031880061 |
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| 100 | 1 | |a Boyle, Natalie K. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
| 264 | 1 | |c 2018 | |
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| 520 | |a Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. | ||
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| 650 | 4 | |a retention |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a pollination |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Machtley, Scott A. |e verfasserin |4 aut | |
| 700 | 1 | |a Hagler, James R. |e verfasserin |4 aut | |
| 700 | 1 | |a Pitts-Singer, Theresa L. |e verfasserin |4 aut | |
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10.1007/s13592-018-0564-4 doi (DE-627)SPR031880061 (SPR)s13592-018-0564-4-e DE-627 ger DE-627 rakwb eng 630 640 590 ASE 42.00 bkl 48.00 bkl Boyle, Natalie K. verfasserin aut Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 Machtley, Scott A. verfasserin aut Hagler, James R. verfasserin aut Pitts-Singer, Theresa L. verfasserin aut Enthalten in Apidologie Paris : Springer, 1970 49(2018), 3 vom: 28. Feb., Seite 378-385 (DE-627)312842996 (DE-600)2005148-7 1297-9678 nnns volume:49 year:2018 number:3 day:28 month:02 pages:378-385 https://dx.doi.org/10.1007/s13592-018-0564-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE 48.00 ASE AR 49 2018 3 28 02 378-385 |
| spelling |
10.1007/s13592-018-0564-4 doi (DE-627)SPR031880061 (SPR)s13592-018-0564-4-e DE-627 ger DE-627 rakwb eng 630 640 590 ASE 42.00 bkl 48.00 bkl Boyle, Natalie K. verfasserin aut Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 Machtley, Scott A. verfasserin aut Hagler, James R. verfasserin aut Pitts-Singer, Theresa L. verfasserin aut Enthalten in Apidologie Paris : Springer, 1970 49(2018), 3 vom: 28. Feb., Seite 378-385 (DE-627)312842996 (DE-600)2005148-7 1297-9678 nnns volume:49 year:2018 number:3 day:28 month:02 pages:378-385 https://dx.doi.org/10.1007/s13592-018-0564-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE 48.00 ASE AR 49 2018 3 28 02 378-385 |
| allfields_unstemmed |
10.1007/s13592-018-0564-4 doi (DE-627)SPR031880061 (SPR)s13592-018-0564-4-e DE-627 ger DE-627 rakwb eng 630 640 590 ASE 42.00 bkl 48.00 bkl Boyle, Natalie K. verfasserin aut Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 Machtley, Scott A. verfasserin aut Hagler, James R. verfasserin aut Pitts-Singer, Theresa L. verfasserin aut Enthalten in Apidologie Paris : Springer, 1970 49(2018), 3 vom: 28. Feb., Seite 378-385 (DE-627)312842996 (DE-600)2005148-7 1297-9678 nnns volume:49 year:2018 number:3 day:28 month:02 pages:378-385 https://dx.doi.org/10.1007/s13592-018-0564-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE 48.00 ASE AR 49 2018 3 28 02 378-385 |
| allfieldsGer |
10.1007/s13592-018-0564-4 doi (DE-627)SPR031880061 (SPR)s13592-018-0564-4-e DE-627 ger DE-627 rakwb eng 630 640 590 ASE 42.00 bkl 48.00 bkl Boyle, Natalie K. verfasserin aut Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 Machtley, Scott A. verfasserin aut Hagler, James R. verfasserin aut Pitts-Singer, Theresa L. verfasserin aut Enthalten in Apidologie Paris : Springer, 1970 49(2018), 3 vom: 28. Feb., Seite 378-385 (DE-627)312842996 (DE-600)2005148-7 1297-9678 nnns volume:49 year:2018 number:3 day:28 month:02 pages:378-385 https://dx.doi.org/10.1007/s13592-018-0564-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE 48.00 ASE AR 49 2018 3 28 02 378-385 |
| allfieldsSound |
10.1007/s13592-018-0564-4 doi (DE-627)SPR031880061 (SPR)s13592-018-0564-4-e DE-627 ger DE-627 rakwb eng 630 640 590 ASE 42.00 bkl 48.00 bkl Boyle, Natalie K. verfasserin aut Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 Machtley, Scott A. verfasserin aut Hagler, James R. verfasserin aut Pitts-Singer, Theresa L. verfasserin aut Enthalten in Apidologie Paris : Springer, 1970 49(2018), 3 vom: 28. Feb., Seite 378-385 (DE-627)312842996 (DE-600)2005148-7 1297-9678 nnns volume:49 year:2018 number:3 day:28 month:02 pages:378-385 https://dx.doi.org/10.1007/s13592-018-0564-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OPC-FOR SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE 48.00 ASE AR 49 2018 3 28 02 378-385 |
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English |
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Enthalten in Apidologie 49(2018), 3 vom: 28. Feb., Seite 378-385 volume:49 year:2018 number:3 day:28 month:02 pages:378-385 |
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Enthalten in Apidologie 49(2018), 3 vom: 28. Feb., Seite 378-385 volume:49 year:2018 number:3 day:28 month:02 pages:378-385 |
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protein marking ELISA dispersal retention pollination |
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Apidologie |
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Boyle, Natalie K. @@aut@@ Machtley, Scott A. @@aut@@ Hagler, James R. @@aut@@ Pitts-Singer, Theresa L. @@aut@@ |
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2018-02-28T00:00:00Z |
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Boyle, Natalie K. |
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Boyle, Natalie K. ddc 630 bkl 42.00 bkl 48.00 misc protein marking misc ELISA misc dispersal misc retention misc pollination Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
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630 640 590 ASE 42.00 bkl 48.00 bkl Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies protein marking (dpeaa)DE-He213 ELISA (dpeaa)DE-He213 dispersal (dpeaa)DE-He213 retention (dpeaa)DE-He213 pollination (dpeaa)DE-He213 |
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ddc 630 bkl 42.00 bkl 48.00 misc protein marking misc ELISA misc dispersal misc retention misc pollination |
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Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
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Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
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Boyle, Natalie K. |
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Boyle, Natalie K. Machtley, Scott A. Hagler, James R. Pitts-Singer, Theresa L. |
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evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, osmia lignaria (hymenoptera: megachilidae), for mark-capture studies |
| title_auth |
Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
| abstract |
Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. |
| abstractGer |
Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. |
| abstract_unstemmed |
Abstract The managed, solitary bee, Osmia lignaria Say, is an efficient pollinator of orchard crops. One limitation to their commercial success is high dispersal of populations away from orchards, resulting in low establishment in provided nest cavities even when optimal pollination is achieved. While exact causes for dispersal are unknown, many existing theories remain untested due to complications with evaluating O. lignaria establishment in field settings. We describe relatively simple and reliable methods for passively marking O. lignaria with egg albumin and casein powdered proteins combined with orange fluorescent powder as they emerge from cocoons. Marked adults were examined microscopically at 0, 3, 6, 9, 12, 15, and 18 days after emergence for the fluorescent powder followed by an anti-albumin and anti-casein enzyme-linked immunosorbent assay to detect the protein powders. The orange powder and egg albumin were highly persistent on adults throughout the 18-day study, suggesting the utility of these markers for studying dispersal and retention of O. lignaria in the field. |
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3 |
| title_short |
Evaluating the persistence of fluorescent and protein powders on adult blue orchard bees, Osmia lignaria (Hymenoptera: Megachilidae), for mark-capture studies |
| url |
https://dx.doi.org/10.1007/s13592-018-0564-4 |
| remote_bool |
true |
| author2 |
Machtley, Scott A. Hagler, James R. Pitts-Singer, Theresa L. |
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Machtley, Scott A. Hagler, James R. Pitts-Singer, Theresa L. |
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| doi_str |
10.1007/s13592-018-0564-4 |
| up_date |
2024-07-04T01:38:51.312Z |
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| score |
7.401394 |