Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan

Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Shafquat, Yusra [verfasserIn] Jabeen, Kauser [verfasserIn] Farooqi, Joveria [verfasserIn] Mehmood, Kiran [verfasserIn] Irfan, Seema [verfasserIn] Hasan, Rumina [verfasserIn] Zafar, Afia [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Anaerobic antimicrobial susceptibility Anaerobic resistance to metronidazole Anaerobic resistance to carbapenem Risk factors associated with resistance

Übergeordnetes Werk:	Enthalten in: Antimicrobial resistance and infection control - London : Biomed Central, 2012, 8(2019), 1 vom: 14. Juni
Übergeordnetes Werk:	volume:8 ; year:2019 ; number:1 ; day:14 ; month:06

Links:	Volltext

DOI / URN:	10.1186/s13756-019-0549-8

Katalog-ID:	SPR03228618X

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520			\|a Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan.
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allfields	10.1186/s13756-019-0549-8 doi (DE-627)SPR03228618X (SPR)s13756-019-0549-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.38 bkl 44.75 bkl Shafquat, Yusra verfasserin aut Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213 Jabeen, Kauser verfasserin aut Farooqi, Joveria verfasserin aut Mehmood, Kiran verfasserin aut Irfan, Seema verfasserin aut Hasan, Rumina verfasserin aut Zafar, Afia verfasserin aut Enthalten in Antimicrobial resistance and infection control London : Biomed Central, 2012 8(2019), 1 vom: 14. Juni (DE-627)718716140 (DE-600)2666706-X 2047-2994 nnns volume:8 year:2019 number:1 day:14 month:06 https://dx.doi.org/10.1186/s13756-019-0549-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.38 ASE 44.75 ASE AR 8 2019 1 14 06
spelling	10.1186/s13756-019-0549-8 doi (DE-627)SPR03228618X (SPR)s13756-019-0549-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.38 bkl 44.75 bkl Shafquat, Yusra verfasserin aut Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213 Jabeen, Kauser verfasserin aut Farooqi, Joveria verfasserin aut Mehmood, Kiran verfasserin aut Irfan, Seema verfasserin aut Hasan, Rumina verfasserin aut Zafar, Afia verfasserin aut Enthalten in Antimicrobial resistance and infection control London : Biomed Central, 2012 8(2019), 1 vom: 14. Juni (DE-627)718716140 (DE-600)2666706-X 2047-2994 nnns volume:8 year:2019 number:1 day:14 month:06 https://dx.doi.org/10.1186/s13756-019-0549-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.38 ASE 44.75 ASE AR 8 2019 1 14 06
allfields_unstemmed	10.1186/s13756-019-0549-8 doi (DE-627)SPR03228618X (SPR)s13756-019-0549-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.38 bkl 44.75 bkl Shafquat, Yusra verfasserin aut Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213 Jabeen, Kauser verfasserin aut Farooqi, Joveria verfasserin aut Mehmood, Kiran verfasserin aut Irfan, Seema verfasserin aut Hasan, Rumina verfasserin aut Zafar, Afia verfasserin aut Enthalten in Antimicrobial resistance and infection control London : Biomed Central, 2012 8(2019), 1 vom: 14. Juni (DE-627)718716140 (DE-600)2666706-X 2047-2994 nnns volume:8 year:2019 number:1 day:14 month:06 https://dx.doi.org/10.1186/s13756-019-0549-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.38 ASE 44.75 ASE AR 8 2019 1 14 06
allfieldsGer	10.1186/s13756-019-0549-8 doi (DE-627)SPR03228618X (SPR)s13756-019-0549-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.38 bkl 44.75 bkl Shafquat, Yusra verfasserin aut Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213 Jabeen, Kauser verfasserin aut Farooqi, Joveria verfasserin aut Mehmood, Kiran verfasserin aut Irfan, Seema verfasserin aut Hasan, Rumina verfasserin aut Zafar, Afia verfasserin aut Enthalten in Antimicrobial resistance and infection control London : Biomed Central, 2012 8(2019), 1 vom: 14. Juni (DE-627)718716140 (DE-600)2666706-X 2047-2994 nnns volume:8 year:2019 number:1 day:14 month:06 https://dx.doi.org/10.1186/s13756-019-0549-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.38 ASE 44.75 ASE AR 8 2019 1 14 06
allfieldsSound	10.1186/s13756-019-0549-8 doi (DE-627)SPR03228618X (SPR)s13756-019-0549-8-e DE-627 ger DE-627 rakwb eng 610 ASE 44.38 bkl 44.75 bkl Shafquat, Yusra verfasserin aut Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan. Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213 Jabeen, Kauser verfasserin aut Farooqi, Joveria verfasserin aut Mehmood, Kiran verfasserin aut Irfan, Seema verfasserin aut Hasan, Rumina verfasserin aut Zafar, Afia verfasserin aut Enthalten in Antimicrobial resistance and infection control London : Biomed Central, 2012 8(2019), 1 vom: 14. Juni (DE-627)718716140 (DE-600)2666706-X 2047-2994 nnns volume:8 year:2019 number:1 day:14 month:06 https://dx.doi.org/10.1186/s13756-019-0549-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.38 ASE 44.75 ASE AR 8 2019 1 14 06
language	English
source	Enthalten in Antimicrobial resistance and infection control 8(2019), 1 vom: 14. Juni volume:8 year:2019 number:1 day:14 month:06
sourceStr	Enthalten in Antimicrobial resistance and infection control 8(2019), 1 vom: 14. Juni volume:8 year:2019 number:1 day:14 month:06
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Anaerobic antimicrobial susceptibility Anaerobic resistance to metronidazole Anaerobic resistance to carbapenem Risk factors associated with resistance
dewey-raw	610
isfreeaccess_bool	true
container_title	Antimicrobial resistance and infection control
authorswithroles_txt_mv	Shafquat, Yusra @@aut@@ Jabeen, Kauser @@aut@@ Farooqi, Joveria @@aut@@ Mehmood, Kiran @@aut@@ Irfan, Seema @@aut@@ Hasan, Rumina @@aut@@ Zafar, Afia @@aut@@
publishDateDaySort_date	2019-06-14T00:00:00Z
hierarchy_top_id	718716140
dewey-sort	3610
id	SPR03228618X
language_de	englisch
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In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. 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author	Shafquat, Yusra
spellingShingle	Shafquat, Yusra ddc 610 bkl 44.38 bkl 44.75 misc Anaerobic antimicrobial susceptibility misc Anaerobic resistance to metronidazole misc Anaerobic resistance to carbapenem misc Risk factors associated with resistance Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
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topic_title	610 ASE 44.38 bkl 44.75 bkl Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan Anaerobic antimicrobial susceptibility (dpeaa)DE-He213 Anaerobic resistance to metronidazole (dpeaa)DE-He213 Anaerobic resistance to carbapenem (dpeaa)DE-He213 Risk factors associated with resistance (dpeaa)DE-He213
topic	ddc 610 bkl 44.38 bkl 44.75 misc Anaerobic antimicrobial susceptibility misc Anaerobic resistance to metronidazole misc Anaerobic resistance to carbapenem misc Risk factors associated with resistance
topic_unstemmed	ddc 610 bkl 44.38 bkl 44.75 misc Anaerobic antimicrobial susceptibility misc Anaerobic resistance to metronidazole misc Anaerobic resistance to carbapenem misc Risk factors associated with resistance
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title	Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
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title_full	Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
author_sort	Shafquat, Yusra
journal	Antimicrobial resistance and infection control
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author_browse	Shafquat, Yusra Jabeen, Kauser Farooqi, Joveria Mehmood, Kiran Irfan, Seema Hasan, Rumina Zafar, Afia
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title_sort	antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from pakistan
title_auth	Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
abstract	Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan.
abstractGer	Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan.
abstract_unstemmed	Background Globally metronidazole and carbapenem resistance in anaerobic organisms is increasing necessitating continuous surveillance to guide selection of empirical treatment. In this study we have determined metronidazole resistance in anaerobes using MIC Evaluator strips (M.I.C.E strips). Carbapenem resistance was evaluated only in metronidazole resistant isolates. Material and methods The study was conducted at the Aga Khan University (AKU) Hospital laboratory, Karachi, Pakistan (2014–2017). Metronidazole and imipenem resistance was evaluated using M.I.C.E strips and minimum inhibitory concentrations (MICs) were interpreted using Clinical Laboratory Standards Institute (CLSI) criteria. Clinical details including demographics, prolonged hospital stay, malignancy, transplant, dialysis, diabetes, site of infection and outcome were analyzed for association with metronidazole resistance. Results Of the 223 clinically significant isolates, 39 (17.5%) were metronidazole resistant (excluding the inherently resistant organisms; for example Cutibacterium species). Imipenem resistance was determined in 29 metronidazole resistant isolates and of these 7 (24.1%) were found to be resistant. Proportion of metronidazole resistant strains was highest amongst Bacteroides species. A significant increase in metronidazole resistance from 12.3% in 2010–2011 to 17.5% in the current study was found. Carbapenem resistance also emerged in the period 2014–2017. Isolates from malignancy and transplant patients showed lower odds of developing metronidazole resistance (0.003(95% CI: 1.7–17.9)). Prolonged hospital stay was not associated with metronidazole resistance (1.1((95% CI: 0.5–2.5)). Conclusion The rising trend of metronidazole resistance and emergence of carbapenem resistance in anaerobic bacteria is alarming. Continued surveillance with strengthening of laboratory capacity regarding anaerobic susceptibility testing is urgently needed in Pakistan.
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title_short	Antimicrobial susceptibility against metronidazole and carbapenem in clinical anaerobic isolates from Pakistan
url	https://dx.doi.org/10.1186/s13756-019-0549-8
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author2	Jabeen, Kauser Farooqi, Joveria Mehmood, Kiran Irfan, Seema Hasan, Rumina Zafar, Afia
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