Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport cond...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yu, Na-Hee [verfasserIn] Chun, So Young [verfasserIn] Ha, Yun-Sok [verfasserIn] Kim, Hyun Tae [verfasserIn] Kim, Dae Hwan [verfasserIn] Kim, Jeongshik [verfasserIn] Chung, Jae-Wook [verfasserIn] Lee, Jun Nyung [verfasserIn] Song, Phil Hyun [verfasserIn] Yoo, Eun Sang [verfasserIn] Kim, Bum Soo [verfasserIn] Kwon, Tae Gyun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Tissue Engineering and Regenerative Medicine - Springer Netherlands, 2012, 15(2018), 5 vom: 03. Juli, Seite 639-647 |
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| Übergeordnetes Werk: |
volume:15 ; year:2018 ; number:5 ; day:03 ; month:07 ; pages:639-647 |
| Links: |
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| DOI / URN: |
10.1007/s13770-018-0133-y |
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SPR032350228 |
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| 520 | |a BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. | ||
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| 700 | 1 | |a Chun, So Young |e verfasserin |4 aut | |
| 700 | 1 | |a Ha, Yun-Sok |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Hyun Tae |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Dae Hwan |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jeongshik |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Jae-Wook |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jun Nyung |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Phil Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Yoo, Eun Sang |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Bum Soo |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Tae Gyun |e verfasserin |4 aut | |
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10.1007/s13770-018-0133-y doi (DE-627)SPR032350228 (SPR)s13770-018-0133-y-e DE-627 ger DE-627 rakwb eng Yu, Na-Hee verfasserin aut Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Cell transporting (dpeaa)DE-He213 Medium (dpeaa)DE-He213 Cell density (dpeaa)DE-He213 Amniotic fluid stem cell (dpeaa)DE-He213 Chun, So Young verfasserin aut Ha, Yun-Sok verfasserin aut Kim, Hyun Tae verfasserin aut Kim, Dae Hwan verfasserin aut Kim, Jeongshik verfasserin aut Chung, Jae-Wook verfasserin aut Lee, Jun Nyung verfasserin aut Song, Phil Hyun verfasserin aut Yoo, Eun Sang verfasserin aut Kim, Bum Soo verfasserin aut Kwon, Tae Gyun verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 15(2018), 5 vom: 03. Juli, Seite 639-647 (DE-627)SPR032345240 nnns volume:15 year:2018 number:5 day:03 month:07 pages:639-647 https://dx.doi.org/10.1007/s13770-018-0133-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 15 2018 5 03 07 639-647 |
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10.1007/s13770-018-0133-y doi (DE-627)SPR032350228 (SPR)s13770-018-0133-y-e DE-627 ger DE-627 rakwb eng Yu, Na-Hee verfasserin aut Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Cell transporting (dpeaa)DE-He213 Medium (dpeaa)DE-He213 Cell density (dpeaa)DE-He213 Amniotic fluid stem cell (dpeaa)DE-He213 Chun, So Young verfasserin aut Ha, Yun-Sok verfasserin aut Kim, Hyun Tae verfasserin aut Kim, Dae Hwan verfasserin aut Kim, Jeongshik verfasserin aut Chung, Jae-Wook verfasserin aut Lee, Jun Nyung verfasserin aut Song, Phil Hyun verfasserin aut Yoo, Eun Sang verfasserin aut Kim, Bum Soo verfasserin aut Kwon, Tae Gyun verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 15(2018), 5 vom: 03. Juli, Seite 639-647 (DE-627)SPR032345240 nnns volume:15 year:2018 number:5 day:03 month:07 pages:639-647 https://dx.doi.org/10.1007/s13770-018-0133-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 15 2018 5 03 07 639-647 |
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10.1007/s13770-018-0133-y doi (DE-627)SPR032350228 (SPR)s13770-018-0133-y-e DE-627 ger DE-627 rakwb eng Yu, Na-Hee verfasserin aut Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Cell transporting (dpeaa)DE-He213 Medium (dpeaa)DE-He213 Cell density (dpeaa)DE-He213 Amniotic fluid stem cell (dpeaa)DE-He213 Chun, So Young verfasserin aut Ha, Yun-Sok verfasserin aut Kim, Hyun Tae verfasserin aut Kim, Dae Hwan verfasserin aut Kim, Jeongshik verfasserin aut Chung, Jae-Wook verfasserin aut Lee, Jun Nyung verfasserin aut Song, Phil Hyun verfasserin aut Yoo, Eun Sang verfasserin aut Kim, Bum Soo verfasserin aut Kwon, Tae Gyun verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 15(2018), 5 vom: 03. Juli, Seite 639-647 (DE-627)SPR032345240 nnns volume:15 year:2018 number:5 day:03 month:07 pages:639-647 https://dx.doi.org/10.1007/s13770-018-0133-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 15 2018 5 03 07 639-647 |
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10.1007/s13770-018-0133-y doi (DE-627)SPR032350228 (SPR)s13770-018-0133-y-e DE-627 ger DE-627 rakwb eng Yu, Na-Hee verfasserin aut Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Cell transporting (dpeaa)DE-He213 Medium (dpeaa)DE-He213 Cell density (dpeaa)DE-He213 Amniotic fluid stem cell (dpeaa)DE-He213 Chun, So Young verfasserin aut Ha, Yun-Sok verfasserin aut Kim, Hyun Tae verfasserin aut Kim, Dae Hwan verfasserin aut Kim, Jeongshik verfasserin aut Chung, Jae-Wook verfasserin aut Lee, Jun Nyung verfasserin aut Song, Phil Hyun verfasserin aut Yoo, Eun Sang verfasserin aut Kim, Bum Soo verfasserin aut Kwon, Tae Gyun verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 15(2018), 5 vom: 03. Juli, Seite 639-647 (DE-627)SPR032345240 nnns volume:15 year:2018 number:5 day:03 month:07 pages:639-647 https://dx.doi.org/10.1007/s13770-018-0133-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 15 2018 5 03 07 639-647 |
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10.1007/s13770-018-0133-y doi (DE-627)SPR032350228 (SPR)s13770-018-0133-y-e DE-627 ger DE-627 rakwb eng Yu, Na-Hee verfasserin aut Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. Cell transporting (dpeaa)DE-He213 Medium (dpeaa)DE-He213 Cell density (dpeaa)DE-He213 Amniotic fluid stem cell (dpeaa)DE-He213 Chun, So Young verfasserin aut Ha, Yun-Sok verfasserin aut Kim, Hyun Tae verfasserin aut Kim, Dae Hwan verfasserin aut Kim, Jeongshik verfasserin aut Chung, Jae-Wook verfasserin aut Lee, Jun Nyung verfasserin aut Song, Phil Hyun verfasserin aut Yoo, Eun Sang verfasserin aut Kim, Bum Soo verfasserin aut Kwon, Tae Gyun verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 15(2018), 5 vom: 03. Juli, Seite 639-647 (DE-627)SPR032345240 nnns volume:15 year:2018 number:5 day:03 month:07 pages:639-647 https://dx.doi.org/10.1007/s13770-018-0133-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 15 2018 5 03 07 639-647 |
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Yu, Na-Hee @@aut@@ Chun, So Young @@aut@@ Ha, Yun-Sok @@aut@@ Kim, Hyun Tae @@aut@@ Kim, Dae Hwan @@aut@@ Kim, Jeongshik @@aut@@ Chung, Jae-Wook @@aut@@ Lee, Jun Nyung @@aut@@ Song, Phil Hyun @@aut@@ Yoo, Eun Sang @@aut@@ Kim, Bum Soo @@aut@@ Kwon, Tae Gyun @@aut@@ |
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Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. 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Yu, Na-Hee Chun, So Young Ha, Yun-Sok Kim, Hyun Tae Kim, Dae Hwan Kim, Jeongshik Chung, Jae-Wook Lee, Jun Nyung Song, Phil Hyun Yoo, Eun Sang Kim, Bum Soo Kwon, Tae Gyun |
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optimal stem cell transporting conditions to maintain cell viability and characteristics |
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Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics |
| abstract |
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. |
| abstractGer |
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. |
| abstract_unstemmed |
BACKGROUND: The preservation of stem cell viability and characteristics during cell transport from the bench to patients can significantly affect the success of cell therapy. Factors such as suspending medium, time, temperature, cell density, and container type could be considered for transport conditions. METHODS: To establish optimal conditions, human amniotic fluid stem cells’ (AFSCs) viabilities were analyzed under different media {DMEM(H), DMEM/F-12, K-SFM, RPMI 1640, α-MEM, DMEM(L), PBS or saline}, temperature (4, 22 or 37 °C), cell density (1 × $ 10^{7} $ cells were suspended in 0.1, 0.5, 1.0 or 2.0 mL of medium) and container type (plastic syringe or glass bottle). After establishing the transport conditions, stem cell characteristics of AFSCs were compared to freshly prepared cells. RESULTS: Cells transported in DMEM(H) showed relatively higher viability than other media. The optimized transport temperature was 4 °C, and available transport time was within 12 h. A lower cell density was associated with a better survival rate, and a syringe was selected as a transport container because of its clinical convenience. In compare of stem cell characteristics, the transported cells with established conditions showed similar potency as the freshly prepared cells. CONCLUSION: Our findings can provide a foundation to optimization of conditions for stem cell transport. |
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Chun, So Young Ha, Yun-Sok Kim, Hyun Tae Kim, Dae Hwan Kim, Jeongshik Chung, Jae-Wook Lee, Jun Nyung Song, Phil Hyun Yoo, Eun Sang Kim, Bum Soo Kwon, Tae Gyun |
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