Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides
Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide rep...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Islam, Md. Aminul [verfasserIn] Sinha, Pratima [verfasserIn] Sharma, Shyam Sundar [verfasserIn] Negi, Madan Singh [verfasserIn] Tripathi, Shashi Bhushan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Proceedings of the National Academy of Sciences - New York, NY : Springer, 2012, 87(2015), 3 vom: 09. Sept., Seite 727-732 |
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| Übergeordnetes Werk: |
volume:87 ; year:2015 ; number:3 ; day:09 ; month:09 ; pages:727-732 |
| Links: |
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| DOI / URN: |
10.1007/s40011-015-0646-2 |
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| Katalog-ID: |
SPR032632703 |
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| 100 | 1 | |a Islam, Md. Aminul |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
| 264 | 1 | |c 2015 | |
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| 520 | |a Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. | ||
| 650 | 4 | |a Seabuckthorn |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Microsatellite |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a FIASCO |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Polymorphic |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sinha, Pratima |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Shyam Sundar |e verfasserin |4 aut | |
| 700 | 1 | |a Negi, Madan Singh |e verfasserin |4 aut | |
| 700 | 1 | |a Tripathi, Shashi Bhushan |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Proceedings of the National Academy of Sciences |d New York, NY : Springer, 2012 |g 87(2015), 3 vom: 09. Sept., Seite 727-732 |w (DE-627)73921361X |w (DE-600)2707745-7 |x 2250-1746 |7 nnns |
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| 912 | |a GBV_ILN_62 | ||
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| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_73 | ||
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| 912 | |a GBV_ILN_90 | ||
| 912 | |a GBV_ILN_95 | ||
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| 912 | |a GBV_ILN_105 | ||
| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_138 | ||
| 912 | |a GBV_ILN_150 | ||
| 912 | |a GBV_ILN_151 | ||
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| 912 | |a GBV_ILN_171 | ||
| 912 | |a GBV_ILN_187 | ||
| 912 | |a GBV_ILN_213 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_230 | ||
| 912 | |a GBV_ILN_250 | ||
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| 912 | |a GBV_ILN_293 | ||
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| 912 | |a GBV_ILN_602 | ||
| 912 | |a GBV_ILN_636 | ||
| 912 | |a GBV_ILN_702 | ||
| 912 | |a GBV_ILN_2001 | ||
| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
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| 912 | |a GBV_ILN_2007 | ||
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| 912 | |a GBV_ILN_2011 | ||
| 912 | |a GBV_ILN_2014 | ||
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| 912 | |a GBV_ILN_2020 | ||
| 912 | |a GBV_ILN_2021 | ||
| 912 | |a GBV_ILN_2025 | ||
| 912 | |a GBV_ILN_2026 | ||
| 912 | |a GBV_ILN_2027 | ||
| 912 | |a GBV_ILN_2031 | ||
| 912 | |a GBV_ILN_2034 | ||
| 912 | |a GBV_ILN_2037 | ||
| 912 | |a GBV_ILN_2038 | ||
| 912 | |a GBV_ILN_2039 | ||
| 912 | |a GBV_ILN_2044 | ||
| 912 | |a GBV_ILN_2048 | ||
| 912 | |a GBV_ILN_2049 | ||
| 912 | |a GBV_ILN_2050 | ||
| 912 | |a GBV_ILN_2055 | ||
| 912 | |a GBV_ILN_2057 | ||
| 912 | |a GBV_ILN_2059 | ||
| 912 | |a GBV_ILN_2061 | ||
| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2065 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2070 | ||
| 912 | |a GBV_ILN_2086 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
| 912 | |a GBV_ILN_2107 | ||
| 912 | |a GBV_ILN_2108 | ||
| 912 | |a GBV_ILN_2110 | ||
| 912 | |a GBV_ILN_2111 | ||
| 912 | |a GBV_ILN_2112 | ||
| 912 | |a GBV_ILN_2113 | ||
| 912 | |a GBV_ILN_2116 | ||
| 912 | |a GBV_ILN_2118 | ||
| 912 | |a GBV_ILN_2119 | ||
| 912 | |a GBV_ILN_2122 | ||
| 912 | |a GBV_ILN_2129 | ||
| 912 | |a GBV_ILN_2143 | ||
| 912 | |a GBV_ILN_2144 | ||
| 912 | |a GBV_ILN_2147 | ||
| 912 | |a GBV_ILN_2148 | ||
| 912 | |a GBV_ILN_2152 | ||
| 912 | |a GBV_ILN_2153 | ||
| 912 | |a GBV_ILN_2188 | ||
| 912 | |a GBV_ILN_2232 | ||
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| 912 | |a GBV_ILN_2446 | ||
| 912 | |a GBV_ILN_2470 | ||
| 912 | |a GBV_ILN_2472 | ||
| 912 | |a GBV_ILN_2507 | ||
| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_2548 | ||
| 912 | |a GBV_ILN_4035 | ||
| 912 | |a GBV_ILN_4037 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4112 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4242 | ||
| 912 | |a GBV_ILN_4246 | ||
| 912 | |a GBV_ILN_4249 | ||
| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
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| 912 | |a GBV_ILN_4323 | ||
| 912 | |a GBV_ILN_4324 | ||
| 912 | |a GBV_ILN_4325 | ||
| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4336 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
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| 951 | |a AR | ||
| 952 | |d 87 |j 2015 |e 3 |b 09 |c 09 |h 727-732 | ||
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10.1007/s40011-015-0646-2 doi (DE-627)SPR032632703 (SPR)s40011-015-0646-2-e DE-627 ger DE-627 rakwb eng Islam, Md. Aminul verfasserin aut Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 Sinha, Pratima verfasserin aut Sharma, Shyam Sundar verfasserin aut Negi, Madan Singh verfasserin aut Tripathi, Shashi Bhushan verfasserin aut Enthalten in Proceedings of the National Academy of Sciences New York, NY : Springer, 2012 87(2015), 3 vom: 09. Sept., Seite 727-732 (DE-627)73921361X (DE-600)2707745-7 2250-1746 nnns volume:87 year:2015 number:3 day:09 month:09 pages:727-732 https://dx.doi.org/10.1007/s40011-015-0646-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 87 2015 3 09 09 727-732 |
| spelling |
10.1007/s40011-015-0646-2 doi (DE-627)SPR032632703 (SPR)s40011-015-0646-2-e DE-627 ger DE-627 rakwb eng Islam, Md. Aminul verfasserin aut Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 Sinha, Pratima verfasserin aut Sharma, Shyam Sundar verfasserin aut Negi, Madan Singh verfasserin aut Tripathi, Shashi Bhushan verfasserin aut Enthalten in Proceedings of the National Academy of Sciences New York, NY : Springer, 2012 87(2015), 3 vom: 09. Sept., Seite 727-732 (DE-627)73921361X (DE-600)2707745-7 2250-1746 nnns volume:87 year:2015 number:3 day:09 month:09 pages:727-732 https://dx.doi.org/10.1007/s40011-015-0646-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 87 2015 3 09 09 727-732 |
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10.1007/s40011-015-0646-2 doi (DE-627)SPR032632703 (SPR)s40011-015-0646-2-e DE-627 ger DE-627 rakwb eng Islam, Md. Aminul verfasserin aut Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 Sinha, Pratima verfasserin aut Sharma, Shyam Sundar verfasserin aut Negi, Madan Singh verfasserin aut Tripathi, Shashi Bhushan verfasserin aut Enthalten in Proceedings of the National Academy of Sciences New York, NY : Springer, 2012 87(2015), 3 vom: 09. Sept., Seite 727-732 (DE-627)73921361X (DE-600)2707745-7 2250-1746 nnns volume:87 year:2015 number:3 day:09 month:09 pages:727-732 https://dx.doi.org/10.1007/s40011-015-0646-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 87 2015 3 09 09 727-732 |
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10.1007/s40011-015-0646-2 doi (DE-627)SPR032632703 (SPR)s40011-015-0646-2-e DE-627 ger DE-627 rakwb eng Islam, Md. Aminul verfasserin aut Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 Sinha, Pratima verfasserin aut Sharma, Shyam Sundar verfasserin aut Negi, Madan Singh verfasserin aut Tripathi, Shashi Bhushan verfasserin aut Enthalten in Proceedings of the National Academy of Sciences New York, NY : Springer, 2012 87(2015), 3 vom: 09. Sept., Seite 727-732 (DE-627)73921361X (DE-600)2707745-7 2250-1746 nnns volume:87 year:2015 number:3 day:09 month:09 pages:727-732 https://dx.doi.org/10.1007/s40011-015-0646-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 87 2015 3 09 09 727-732 |
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10.1007/s40011-015-0646-2 doi (DE-627)SPR032632703 (SPR)s40011-015-0646-2-e DE-627 ger DE-627 rakwb eng Islam, Md. Aminul verfasserin aut Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 Sinha, Pratima verfasserin aut Sharma, Shyam Sundar verfasserin aut Negi, Madan Singh verfasserin aut Tripathi, Shashi Bhushan verfasserin aut Enthalten in Proceedings of the National Academy of Sciences New York, NY : Springer, 2012 87(2015), 3 vom: 09. Sept., Seite 727-732 (DE-627)73921361X (DE-600)2707745-7 2250-1746 nnns volume:87 year:2015 number:3 day:09 month:09 pages:727-732 https://dx.doi.org/10.1007/s40011-015-0646-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 87 2015 3 09 09 727-732 |
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Islam, Md. Aminul @@aut@@ Sinha, Pratima @@aut@@ Sharma, Shyam Sundar @@aut@@ Negi, Madan Singh @@aut@@ Tripathi, Shashi Bhushan @@aut@@ |
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Aminul</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. 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Islam, Md. Aminul |
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Islam, Md. Aminul misc Seabuckthorn misc Microsatellite misc FIASCO misc Polymorphic Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
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Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides Seabuckthorn (dpeaa)DE-He213 Microsatellite (dpeaa)DE-He213 FIASCO (dpeaa)DE-He213 Polymorphic (dpeaa)DE-He213 |
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Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
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Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
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Islam, Md. Aminul |
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Islam, Md. Aminul Sinha, Pratima Sharma, Shyam Sundar Negi, Madan Singh Tripathi, Shashi Bhushan |
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Islam, Md. Aminul |
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isolation and characterization of novel polymorphic microsatellite loci in hippophae rhamnoides |
| title_auth |
Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
| abstract |
Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. |
| abstractGer |
Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. |
| abstract_unstemmed |
Abstract Hippophae rhamnoides L. belonging to family Elaeagnaceae has been a species of interest due to its high nutritional value. In the present study, a set of 26 novel microsatellites from Hippophae developed from an enriched genomic library using biotin labelled di, tri and tetra nucleotide repeats have been reported. Out of 108 clones sequenced, only 62 fragments comprised of microsatellites. All the 26 designed primers showed amplification and 14 highly polymorphic markers were selected and genotyped in 24 Hippophae accessions. The number of alleles ranged from 3 to 7 alleles per locus with an average of 5. The average genetic distance among these accessions was 0.64. Polymorphic information content ranged from 0.47 to 0.79. Mean expected heterozygosity ($ H_{e} $) and observed heterozygosity ($ H_{o} $) were 0.69 and 0.43 respectively. Hardy–Weinberg and Linkage disequilibrium showed that all 14 polymorphic loci were in HWE (P < 0.005) with significant linkage disequilibrium between pairs of loci. These new sets of microsatellite markers are expected to be especially useful in future for linkage mapping, varietal identification and genetic diversity studies. |
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| title_short |
Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides |
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https://dx.doi.org/10.1007/s40011-015-0646-2 |
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Sinha, Pratima Sharma, Shyam Sundar Negi, Madan Singh Tripathi, Shashi Bhushan |
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Sinha, Pratima Sharma, Shyam Sundar Negi, Madan Singh Tripathi, Shashi Bhushan |
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| doi_str |
10.1007/s40011-015-0646-2 |
| up_date |
2024-07-03T13:57:25.190Z |
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| score |
7.4009523 |