Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach

Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municip...
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Gespeichert in:

Autor*in:	Bhatia, Mayuri [verfasserIn] Girdhar, Amandeep [verfasserIn] Tiwari, Archana [verfasserIn] Nayarisseri, Anuraj [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	LDPE Biodegradation EMBS027 LDPE degrading species

Übergeordnetes Werk:	Enthalten in: SpringerPlus - London : Biomed Central, 2012, 3(2014), 1 vom: 02. Sept.
Übergeordnetes Werk:	volume:3 ; year:2014 ; number:1 ; day:02 ; month:09

Links:	Volltext

DOI / URN:	10.1186/2193-1801-3-497

Katalog-ID:	SPR032759762

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allfields	10.1186/2193-1801-3-497 doi (DE-627)SPR032759762 (SPR)2193-1801-3-497-e DE-627 ger DE-627 rakwb eng 600 ASE Bhatia, Mayuri verfasserin aut Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478. LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213 Girdhar, Amandeep verfasserin aut Tiwari, Archana verfasserin aut Nayarisseri, Anuraj verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 3(2014), 1 vom: 02. Sept. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:3 year:2014 number:1 day:02 month:09 https://dx.doi.org/10.1186/2193-1801-3-497 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014 1 02 09
spelling	10.1186/2193-1801-3-497 doi (DE-627)SPR032759762 (SPR)2193-1801-3-497-e DE-627 ger DE-627 rakwb eng 600 ASE Bhatia, Mayuri verfasserin aut Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478. LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213 Girdhar, Amandeep verfasserin aut Tiwari, Archana verfasserin aut Nayarisseri, Anuraj verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 3(2014), 1 vom: 02. Sept. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:3 year:2014 number:1 day:02 month:09 https://dx.doi.org/10.1186/2193-1801-3-497 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014 1 02 09
allfields_unstemmed	10.1186/2193-1801-3-497 doi (DE-627)SPR032759762 (SPR)2193-1801-3-497-e DE-627 ger DE-627 rakwb eng 600 ASE Bhatia, Mayuri verfasserin aut Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478. LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213 Girdhar, Amandeep verfasserin aut Tiwari, Archana verfasserin aut Nayarisseri, Anuraj verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 3(2014), 1 vom: 02. Sept. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:3 year:2014 number:1 day:02 month:09 https://dx.doi.org/10.1186/2193-1801-3-497 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014 1 02 09
allfieldsGer	10.1186/2193-1801-3-497 doi (DE-627)SPR032759762 (SPR)2193-1801-3-497-e DE-627 ger DE-627 rakwb eng 600 ASE Bhatia, Mayuri verfasserin aut Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478. LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213 Girdhar, Amandeep verfasserin aut Tiwari, Archana verfasserin aut Nayarisseri, Anuraj verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 3(2014), 1 vom: 02. Sept. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:3 year:2014 number:1 day:02 month:09 https://dx.doi.org/10.1186/2193-1801-3-497 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014 1 02 09
allfieldsSound	10.1186/2193-1801-3-497 doi (DE-627)SPR032759762 (SPR)2193-1801-3-497-e DE-627 ger DE-627 rakwb eng 600 ASE Bhatia, Mayuri verfasserin aut Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478. LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213 Girdhar, Amandeep verfasserin aut Tiwari, Archana verfasserin aut Nayarisseri, Anuraj verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 3(2014), 1 vom: 02. Sept. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:3 year:2014 number:1 day:02 month:09 https://dx.doi.org/10.1186/2193-1801-3-497 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2014 1 02 09
language	English
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topic_facet	LDPE Biodegradation EMBS027 LDPE degrading species
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container_title	SpringerPlus
authorswithroles_txt_mv	Bhatia, Mayuri @@aut@@ Girdhar, Amandeep @@aut@@ Tiwari, Archana @@aut@@ Nayarisseri, Anuraj @@aut@@
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author	Bhatia, Mayuri
spellingShingle	Bhatia, Mayuri ddc 600 misc LDPE misc Biodegradation misc EMBS027 misc LDPE degrading species Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
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topic_title	600 ASE Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach LDPE (dpeaa)DE-He213 Biodegradation (dpeaa)DE-He213 EMBS027 (dpeaa)DE-He213 LDPE degrading species (dpeaa)DE-He213
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title	Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
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title_full	Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
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title_sort	implications of a novel pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
title_auth	Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
abstract	Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478.
abstractGer	Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478.
abstract_unstemmed	Abstract Degradation of Petroleum-plastics like Low Density Polyethylene (LDPE) is a budding challenge due to increasing white pollution. The present investigation has focused the aspect through microbial assisted biodegradation. Various indigenous microorganisms were isolated from collected municipal landfill soil. Growth medium enriched with 0.2 g of LDPE powder was used to screen the soil bacteria with biodegradation potential. The screened bacteria were subjected to biodegradation assay in presence of LDPE sheets in growth medium. Four strains gave 5%, 17.8%, 0.9% and 0.6% degradation rate based on weight loss in the conducted in vitro assay for four days. The maximum degraded sheet was analyzed through Scanning Electron Microscopy, Fourier transform infrared spectroscopy and Thermogravimetry, taking undegraded LDPE sheet as control. Results illustrated one-step weight loss with control and three-step weight loss with test. Thus, it proved the efficacy of isolated strain. The strain identification was carried out by genomic DNA isolation followed by PCR and 16S rRNA sequencing. Genotypic identification revealed the bacterium as Pseudomonas citronellolis. BLAST gave a similarity with the database of 96%, thus phylogenetic assessment clarified the bacterium as a novel strain. The isolate was named as Pseudomonas citronellolis EMBS027 and sequence was deposited as LDPE degrading species, in GenBank with accession number KF361478.
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title_short	Implications of a novel Pseudomonas species on low density polyethylene biodegradation: an in vitro to in silico approach
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author2	Girdhar, Amandeep Tiwari, Archana Nayarisseri, Anuraj
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