Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis

Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously exp...
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Autor*in:	Tang, Xia [verfasserIn] Chen, Jia [verfasserIn] Wang, Ying [verfasserIn] Wang, Xianchun [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Rab3A Fusion expression Polyclonal antibody Gel protein recovery Protein interaction

Übergeordnetes Werk:	Enthalten in: SpringerPlus - London : Biomed Central, 2012, 5(2016), 1 vom: 03. Okt.
Übergeordnetes Werk:	volume:5 ; year:2016 ; number:1 ; day:03 ; month:10

Links:	Volltext

DOI / URN:	10.1186/s40064-016-3330-y

Katalog-ID:	SPR032793138

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520			\|a Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies.
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matchkey_str	article:21931801:2016----::eelnnepesoadoylnlnioyrprtoorbaopo
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allfields	10.1186/s40064-016-3330-y doi (DE-627)SPR032793138 (SPR)s40064-016-3330-y-e DE-627 ger DE-627 rakwb eng 600 ASE Tang, Xia verfasserin aut Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies. Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213 Chen, Jia verfasserin aut Wang, Ying verfasserin aut Wang, Xianchun verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 5(2016), 1 vom: 03. Okt. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:5 year:2016 number:1 day:03 month:10 https://dx.doi.org/10.1186/s40064-016-3330-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016 1 03 10
spelling	10.1186/s40064-016-3330-y doi (DE-627)SPR032793138 (SPR)s40064-016-3330-y-e DE-627 ger DE-627 rakwb eng 600 ASE Tang, Xia verfasserin aut Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies. Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213 Chen, Jia verfasserin aut Wang, Ying verfasserin aut Wang, Xianchun verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 5(2016), 1 vom: 03. Okt. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:5 year:2016 number:1 day:03 month:10 https://dx.doi.org/10.1186/s40064-016-3330-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016 1 03 10
allfields_unstemmed	10.1186/s40064-016-3330-y doi (DE-627)SPR032793138 (SPR)s40064-016-3330-y-e DE-627 ger DE-627 rakwb eng 600 ASE Tang, Xia verfasserin aut Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies. Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213 Chen, Jia verfasserin aut Wang, Ying verfasserin aut Wang, Xianchun verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 5(2016), 1 vom: 03. Okt. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:5 year:2016 number:1 day:03 month:10 https://dx.doi.org/10.1186/s40064-016-3330-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016 1 03 10
allfieldsGer	10.1186/s40064-016-3330-y doi (DE-627)SPR032793138 (SPR)s40064-016-3330-y-e DE-627 ger DE-627 rakwb eng 600 ASE Tang, Xia verfasserin aut Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies. Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213 Chen, Jia verfasserin aut Wang, Ying verfasserin aut Wang, Xianchun verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 5(2016), 1 vom: 03. Okt. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:5 year:2016 number:1 day:03 month:10 https://dx.doi.org/10.1186/s40064-016-3330-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016 1 03 10
allfieldsSound	10.1186/s40064-016-3330-y doi (DE-627)SPR032793138 (SPR)s40064-016-3330-y-e DE-627 ger DE-627 rakwb eng 600 ASE Tang, Xia verfasserin aut Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies. Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213 Chen, Jia verfasserin aut Wang, Ying verfasserin aut Wang, Xianchun verfasserin aut Enthalten in SpringerPlus London : Biomed Central, 2012 5(2016), 1 vom: 03. Okt. (DE-627)718615298 (DE-600)2661116-8 2193-1801 nnns volume:5 year:2016 number:1 day:03 month:10 https://dx.doi.org/10.1186/s40064-016-3330-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 2016 1 03 10
language	English
source	Enthalten in SpringerPlus 5(2016), 1 vom: 03. Okt. volume:5 year:2016 number:1 day:03 month:10
sourceStr	Enthalten in SpringerPlus 5(2016), 1 vom: 03. Okt. volume:5 year:2016 number:1 day:03 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Rab3A Fusion expression Polyclonal antibody Gel protein recovery Protein interaction
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isfreeaccess_bool	true
container_title	SpringerPlus
authorswithroles_txt_mv	Tang, Xia @@aut@@ Chen, Jia @@aut@@ Wang, Ying @@aut@@ Wang, Xianchun @@aut@@
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Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. 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author	Tang, Xia
spellingShingle	Tang, Xia ddc 600 misc Rab3A misc Fusion expression misc Polyclonal antibody misc Gel protein recovery misc Protein interaction Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
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topic_title	600 ASE Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis Rab3A (dpeaa)DE-He213 Fusion expression (dpeaa)DE-He213 Polyclonal antibody (dpeaa)DE-He213 Gel protein recovery (dpeaa)DE-He213 Protein interaction (dpeaa)DE-He213
topic	ddc 600 misc Rab3A misc Fusion expression misc Polyclonal antibody misc Gel protein recovery misc Protein interaction
topic_unstemmed	ddc 600 misc Rab3A misc Fusion expression misc Polyclonal antibody misc Gel protein recovery misc Protein interaction
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title	Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
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title_full	Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
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author_browse	Tang, Xia Chen, Jia Wang, Ying Wang, Xianchun
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title_sort	gene cloning, expression and polyclonal antibody preparation of rab3a for protein interaction analysis
title_auth	Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
abstract	Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies.
abstractGer	Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies.
abstract_unstemmed	Background Rab3A is a GTP-binding protein and plays critical roles in the regulation of synaptic vesicle exocytosis. Up to date, how Rab3A participates in such a regulatory process is not completely clear. Results In this report the Rab3A gene from Rattus norvegicus was cloned and heterologously expressed in E. coli using pCold-TF expression vector with folding capacity. Due to the presence of His-tag sequence on the N-terminal side, Rab3A fusion protein was purified to greater than 95 % purity with a single Ni-affinity purification step. After the Rab3A fusion protein was used to immunize mice, an anti-serum against Rab3A with a titer of about 6000 was generated. Western blot analysis indicated that the prepared polyclonal antibody could recognize both Rab3A fusion protein and native Rab3A protein. To remove the tag sequence, thrombin was used to cleave the Rab3A fusion protein, followed by SDS-PAGE to separate the cleavage products. Using the gel protein recovery strategy with a Micro Protein PAGE Recovery Kit, the de-tagged Rab3A protein of electrophoretic purity was prepared. Conclusions The present work not only prepared the ground for the study on Rab3A-mediated protein interactions, but also provided systematic experimental methods referable for the similar studies.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis
url	https://dx.doi.org/10.1186/s40064-016-3330-y
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author2	Chen, Jia Wang, Ying Wang, Xianchun
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up_date	2024-07-03T14:49:08.626Z
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Gene cloning, expression and polyclonal antibody preparation of Rab3A for protein interaction analysis

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