Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography
Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of t...
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Gespeichert in:
| Autor*in: |
Guo, Donggang [verfasserIn] Ni, Liang [verfasserIn] Wang, Liang [verfasserIn] Shao, Li [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Übergeordnetes Werk: |
Enthalten in: Chemical Research in Chinese Universities - Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012, 35(2019), 2 vom: 21. März, Seite 209-215 |
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| Übergeordnetes Werk: |
volume:35 ; year:2019 ; number:2 ; day:21 ; month:03 ; pages:209-215 |
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| DOI / URN: |
10.1007/s40242-019-8216-4 |
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SPR032920652 |
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| 520 | |a Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. | ||
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| 650 | 4 | |a Partition behavior |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Chloramphenicol |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Extraction efficiency |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Wang, Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Shao, Li |e verfasserin |4 aut | |
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10.1007/s40242-019-8216-4 doi (DE-627)SPR032920652 (SPR)s40242-019-8216-4-e DE-627 ger DE-627 rakwb eng Guo, Donggang verfasserin aut Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. Binary small molecule alcohol aqueous two-phase system (dpeaa)DE-He213 Partition behavior (dpeaa)DE-He213 Chloramphenicol (dpeaa)DE-He213 Extraction efficiency (dpeaa)DE-He213 Ni, Liang verfasserin aut Wang, Liang verfasserin aut Shao, Li verfasserin aut Enthalten in Chemical Research in Chinese Universities Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012 35(2019), 2 vom: 21. März, Seite 209-215 (DE-627)SPR03290777X nnns volume:35 year:2019 number:2 day:21 month:03 pages:209-215 https://dx.doi.org/10.1007/s40242-019-8216-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 35 2019 2 21 03 209-215 |
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10.1007/s40242-019-8216-4 doi (DE-627)SPR032920652 (SPR)s40242-019-8216-4-e DE-627 ger DE-627 rakwb eng Guo, Donggang verfasserin aut Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. Binary small molecule alcohol aqueous two-phase system (dpeaa)DE-He213 Partition behavior (dpeaa)DE-He213 Chloramphenicol (dpeaa)DE-He213 Extraction efficiency (dpeaa)DE-He213 Ni, Liang verfasserin aut Wang, Liang verfasserin aut Shao, Li verfasserin aut Enthalten in Chemical Research in Chinese Universities Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012 35(2019), 2 vom: 21. März, Seite 209-215 (DE-627)SPR03290777X nnns volume:35 year:2019 number:2 day:21 month:03 pages:209-215 https://dx.doi.org/10.1007/s40242-019-8216-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 35 2019 2 21 03 209-215 |
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10.1007/s40242-019-8216-4 doi (DE-627)SPR032920652 (SPR)s40242-019-8216-4-e DE-627 ger DE-627 rakwb eng Guo, Donggang verfasserin aut Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. Binary small molecule alcohol aqueous two-phase system (dpeaa)DE-He213 Partition behavior (dpeaa)DE-He213 Chloramphenicol (dpeaa)DE-He213 Extraction efficiency (dpeaa)DE-He213 Ni, Liang verfasserin aut Wang, Liang verfasserin aut Shao, Li verfasserin aut Enthalten in Chemical Research in Chinese Universities Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012 35(2019), 2 vom: 21. März, Seite 209-215 (DE-627)SPR03290777X nnns volume:35 year:2019 number:2 day:21 month:03 pages:209-215 https://dx.doi.org/10.1007/s40242-019-8216-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 35 2019 2 21 03 209-215 |
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10.1007/s40242-019-8216-4 doi (DE-627)SPR032920652 (SPR)s40242-019-8216-4-e DE-627 ger DE-627 rakwb eng Guo, Donggang verfasserin aut Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. Binary small molecule alcohol aqueous two-phase system (dpeaa)DE-He213 Partition behavior (dpeaa)DE-He213 Chloramphenicol (dpeaa)DE-He213 Extraction efficiency (dpeaa)DE-He213 Ni, Liang verfasserin aut Wang, Liang verfasserin aut Shao, Li verfasserin aut Enthalten in Chemical Research in Chinese Universities Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012 35(2019), 2 vom: 21. März, Seite 209-215 (DE-627)SPR03290777X nnns volume:35 year:2019 number:2 day:21 month:03 pages:209-215 https://dx.doi.org/10.1007/s40242-019-8216-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 35 2019 2 21 03 209-215 |
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10.1007/s40242-019-8216-4 doi (DE-627)SPR032920652 (SPR)s40242-019-8216-4-e DE-627 ger DE-627 rakwb eng Guo, Donggang verfasserin aut Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. Binary small molecule alcohol aqueous two-phase system (dpeaa)DE-He213 Partition behavior (dpeaa)DE-He213 Chloramphenicol (dpeaa)DE-He213 Extraction efficiency (dpeaa)DE-He213 Ni, Liang verfasserin aut Wang, Liang verfasserin aut Shao, Li verfasserin aut Enthalten in Chemical Research in Chinese Universities Jilin University and The Editorial Department of Chemical Research in Chinese Universities, 2012 35(2019), 2 vom: 21. März, Seite 209-215 (DE-627)SPR03290777X nnns volume:35 year:2019 number:2 day:21 month:03 pages:209-215 https://dx.doi.org/10.1007/s40242-019-8216-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA AR 35 2019 2 21 03 209-215 |
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Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography |
| abstract |
Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. |
| abstractGer |
Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. |
| abstract_unstemmed |
Abstract A green and sensitive sample separation and purification method coupled with high-performance liquid chromatography(HPLC) was developed for the analysis of chloramphenicol(CAP). One element small molecule alcohol-salt aqueous two-phase system(ATPS) can’t effectively adjust the polarity of the system, but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. A binary aqueous two-phase system based on 1-propanol+2-propanol and $ NaH_{2} %$ PO_{4} $ system was formed and applied to the separation and purification of trace CAP in real samples. The influence factors on partition behaviors of CAP were discussed, including the types and mass of salts, the volume ratio of alcohol, the pH, temperature and the standing time. The optimal condition was found at pH=5.0, 2.5 g of $ NaH_{2} %$ PO_{4} $, 3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1) and 30 °C by using response surface methodology. Under this optimal condition, the extraction efficiency of CAP reached 98.91%, and partition coefficient of CAP was 17.31. |
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| title_short |
Separation/Extraction/Detection of Chloramphenicol Using Binary Small Molecule Alcohol-Salt Aqueous Two-phase System Coupled with High-performance Liquid Chromatography |
| url |
https://dx.doi.org/10.1007/s40242-019-8216-4 |
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| author2 |
Ni, Liang Wang, Liang Shao, Li |
| author2Str |
Ni, Liang Wang, Liang Shao, Li |
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| doi_str |
10.1007/s40242-019-8216-4 |
| up_date |
2024-07-03T15:27:45.019Z |
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7.4029083 |