Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection

Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Cribbs, Sushma K. [verfasserIn] Uppal, Karan [verfasserIn] Li, Shuzhao [verfasserIn] Jones, Dean P. [verfasserIn] Huang, Laurence [verfasserIn] Tipton, Laura [verfasserIn] Fitch, Adam [verfasserIn] Greenblatt, Ruth M. [verfasserIn] Kingsley, Lawrence [verfasserIn] Guidot, David M. [verfasserIn] Ghedin, Elodie [verfasserIn] Morris, Alison [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	HIV Lung Metabolomics Microbiota Bronchoalveolar lavage

Übergeordnetes Werk:	Enthalten in: Microbiome - London : Biomed Central, 2013, 4(2016), 1 vom: 20. Jan.
Übergeordnetes Werk:	volume:4 ; year:2016 ; number:1 ; day:20 ; month:01

Links:	Volltext

DOI / URN:	10.1186/s40168-016-0147-4

Katalog-ID:	SPR03322384X

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520			\|a Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung.
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allfields	10.1186/s40168-016-0147-4 doi (DE-627)SPR03322384X (SPR)s40168-016-0147-4-e DE-627 ger DE-627 rakwb eng 570 ASE Cribbs, Sushma K. verfasserin aut Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213 Uppal, Karan verfasserin aut Li, Shuzhao verfasserin aut Jones, Dean P. verfasserin aut Huang, Laurence verfasserin aut Tipton, Laura verfasserin aut Fitch, Adam verfasserin aut Greenblatt, Ruth M. verfasserin aut Kingsley, Lawrence verfasserin aut Guidot, David M. verfasserin aut Ghedin, Elodie verfasserin aut Morris, Alison verfasserin aut Enthalten in Microbiome London : Biomed Central, 2013 4(2016), 1 vom: 20. Jan. (DE-627)734146140 (DE-600)2697425-3 2049-2618 nnns volume:4 year:2016 number:1 day:20 month:01 https://dx.doi.org/10.1186/s40168-016-0147-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2016 1 20 01
spelling	10.1186/s40168-016-0147-4 doi (DE-627)SPR03322384X (SPR)s40168-016-0147-4-e DE-627 ger DE-627 rakwb eng 570 ASE Cribbs, Sushma K. verfasserin aut Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213 Uppal, Karan verfasserin aut Li, Shuzhao verfasserin aut Jones, Dean P. verfasserin aut Huang, Laurence verfasserin aut Tipton, Laura verfasserin aut Fitch, Adam verfasserin aut Greenblatt, Ruth M. verfasserin aut Kingsley, Lawrence verfasserin aut Guidot, David M. verfasserin aut Ghedin, Elodie verfasserin aut Morris, Alison verfasserin aut Enthalten in Microbiome London : Biomed Central, 2013 4(2016), 1 vom: 20. Jan. (DE-627)734146140 (DE-600)2697425-3 2049-2618 nnns volume:4 year:2016 number:1 day:20 month:01 https://dx.doi.org/10.1186/s40168-016-0147-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2016 1 20 01
allfields_unstemmed	10.1186/s40168-016-0147-4 doi (DE-627)SPR03322384X (SPR)s40168-016-0147-4-e DE-627 ger DE-627 rakwb eng 570 ASE Cribbs, Sushma K. verfasserin aut Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213 Uppal, Karan verfasserin aut Li, Shuzhao verfasserin aut Jones, Dean P. verfasserin aut Huang, Laurence verfasserin aut Tipton, Laura verfasserin aut Fitch, Adam verfasserin aut Greenblatt, Ruth M. verfasserin aut Kingsley, Lawrence verfasserin aut Guidot, David M. verfasserin aut Ghedin, Elodie verfasserin aut Morris, Alison verfasserin aut Enthalten in Microbiome London : Biomed Central, 2013 4(2016), 1 vom: 20. Jan. (DE-627)734146140 (DE-600)2697425-3 2049-2618 nnns volume:4 year:2016 number:1 day:20 month:01 https://dx.doi.org/10.1186/s40168-016-0147-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2016 1 20 01
allfieldsGer	10.1186/s40168-016-0147-4 doi (DE-627)SPR03322384X (SPR)s40168-016-0147-4-e DE-627 ger DE-627 rakwb eng 570 ASE Cribbs, Sushma K. verfasserin aut Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213 Uppal, Karan verfasserin aut Li, Shuzhao verfasserin aut Jones, Dean P. verfasserin aut Huang, Laurence verfasserin aut Tipton, Laura verfasserin aut Fitch, Adam verfasserin aut Greenblatt, Ruth M. verfasserin aut Kingsley, Lawrence verfasserin aut Guidot, David M. verfasserin aut Ghedin, Elodie verfasserin aut Morris, Alison verfasserin aut Enthalten in Microbiome London : Biomed Central, 2013 4(2016), 1 vom: 20. Jan. (DE-627)734146140 (DE-600)2697425-3 2049-2618 nnns volume:4 year:2016 number:1 day:20 month:01 https://dx.doi.org/10.1186/s40168-016-0147-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2016 1 20 01
allfieldsSound	10.1186/s40168-016-0147-4 doi (DE-627)SPR03322384X (SPR)s40168-016-0147-4-e DE-627 ger DE-627 rakwb eng 570 ASE Cribbs, Sushma K. verfasserin aut Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung. HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213 Uppal, Karan verfasserin aut Li, Shuzhao verfasserin aut Jones, Dean P. verfasserin aut Huang, Laurence verfasserin aut Tipton, Laura verfasserin aut Fitch, Adam verfasserin aut Greenblatt, Ruth M. verfasserin aut Kingsley, Lawrence verfasserin aut Guidot, David M. verfasserin aut Ghedin, Elodie verfasserin aut Morris, Alison verfasserin aut Enthalten in Microbiome London : Biomed Central, 2013 4(2016), 1 vom: 20. Jan. (DE-627)734146140 (DE-600)2697425-3 2049-2618 nnns volume:4 year:2016 number:1 day:20 month:01 https://dx.doi.org/10.1186/s40168-016-0147-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2016 1 20 01
language	English
source	Enthalten in Microbiome 4(2016), 1 vom: 20. Jan. volume:4 year:2016 number:1 day:20 month:01
sourceStr	Enthalten in Microbiome 4(2016), 1 vom: 20. Jan. volume:4 year:2016 number:1 day:20 month:01
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	HIV Lung Metabolomics Microbiota Bronchoalveolar lavage
dewey-raw	570
isfreeaccess_bool	true
container_title	Microbiome
authorswithroles_txt_mv	Cribbs, Sushma K. @@aut@@ Uppal, Karan @@aut@@ Li, Shuzhao @@aut@@ Jones, Dean P. @@aut@@ Huang, Laurence @@aut@@ Tipton, Laura @@aut@@ Fitch, Adam @@aut@@ Greenblatt, Ruth M. @@aut@@ Kingsley, Lawrence @@aut@@ Guidot, David M. @@aut@@ Ghedin, Elodie @@aut@@ Morris, Alison @@aut@@
publishDateDaySort_date	2016-01-20T00:00:00Z
hierarchy_top_id	734146140
dewey-sort	3570
id	SPR03322384X
language_de	englisch
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author	Cribbs, Sushma K.
spellingShingle	Cribbs, Sushma K. ddc 570 misc HIV misc Lung misc Metabolomics misc Microbiota misc Bronchoalveolar lavage Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection
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topic_title	570 ASE Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection HIV (dpeaa)DE-He213 Lung (dpeaa)DE-He213 Metabolomics (dpeaa)DE-He213 Microbiota (dpeaa)DE-He213 Bronchoalveolar lavage (dpeaa)DE-He213
topic	ddc 570 misc HIV misc Lung misc Metabolomics misc Microbiota misc Bronchoalveolar lavage
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title	Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection
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title_full	Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection
author_sort	Cribbs, Sushma K.
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author_browse	Cribbs, Sushma K. Uppal, Karan Li, Shuzhao Jones, Dean P. Huang, Laurence Tipton, Laura Fitch, Adam Greenblatt, Ruth M. Kingsley, Lawrence Guidot, David M. Ghedin, Elodie Morris, Alison
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title_sort	correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in hiv infection
title_auth	Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection
abstract	Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung.
abstractGer	Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung.
abstract_unstemmed	Background While 16S ribosomal RNA (rRNA) sequencing has been used to characterize the lung’s bacterial microbiota in human immunodeficiency virus (HIV)-infected individuals, taxonomic studies provide limited information on bacterial function and impact on the host. Metabolic profiles can provide functional information on host-microbe interactions in the lungs. We investigated the relationship between the respiratory microbiota and metabolic profiles in the bronchoalveolar lavage fluid of HIV-infected and HIV-uninfected outpatients. Results Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. Global integration of all metabolic features with microbial species was done using sparse partial least squares regression. Thirty-nine HIV-infected subjects and 20 HIV-uninfected controls without acute respiratory symptoms were enrolled. Twelve mass-to-charge ratio (m/z) features from C18 analysis were significantly different between HIV-infected individuals and controls (false discovery rate (FDR) = 0.2); another 79 features were identified by network analysis. Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo-l-tyrosine. There were 231 m/z features significantly associated with peripheral blood CD4 cell counts identified using sparse partial least squares regression (sPLS) at a variable importance on projection (VIP) threshold of 2. Twenty-five percent of these 91 m/z features were associated with various microbial species. Bacteria from families Caulobacteraceae, Staphylococcaceae, Nocardioidaceae, and genus Streptococcus were associated with the greatest number of features. Glycerophospholipid and lineolate pathways correlated with these bacteria. Conclusions In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. These findings suggest that microbial communities and their interactions with the host may have functional metabolic impact in the lung.
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title_short	Correlation of the lung microbiota with metabolic profiles in bronchoalveolar lavage fluid in HIV infection
url	https://dx.doi.org/10.1186/s40168-016-0147-4
remote_bool	true
author2	Uppal, Karan Li, Shuzhao Jones, Dean P. Huang, Laurence Tipton, Laura Fitch, Adam Greenblatt, Ruth M. Kingsley, Lawrence Guidot, David M. Ghedin, Elodie Morris, Alison
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