Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation
Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a perm...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bonilla, Victoria [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2003 |
|---|
| Schlagwörter: |
|---|
| Anmerkung: |
© Adis Data Information BV 2003 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Molecular diagnosis & therapy - [S.l.] : Springer International, 2006, 7(2003), 3 vom: Sept., Seite 163-167 |
|---|---|
| Übergeordnetes Werk: |
volume:7 ; year:2003 ; number:3 ; month:09 ; pages:163-167 |
| Links: |
|---|
| DOI / URN: |
10.1007/BF03260033 |
|---|
| Katalog-ID: |
SPR035370351 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | SPR035370351 | ||
| 003 | DE-627 | ||
| 005 | 20230519114445.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201007s2003 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/BF03260033 |2 doi | |
| 035 | |a (DE-627)SPR035370351 | ||
| 035 | |a (SPR)BF03260033-e | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bonilla, Victoria |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| 264 | 1 | |c 2003 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a © Adis Data Information BV 2003 | ||
| 520 | |a Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. | ||
| 650 | 4 | |a Lymphoblastoid Cell Line |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Immortalize Cell |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Full Mutation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Premutated Carrier |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Roche Diagnostics Corporation |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Sobrino, Francisco |4 aut | |
| 700 | 1 | |a Lucas, Miguel |4 aut | |
| 700 | 1 | |a Pintado, Elizabeth |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular diagnosis & therapy |d [S.l.] : Springer International, 2006 |g 7(2003), 3 vom: Sept., Seite 163-167 |w (DE-627)51122799X |w (DE-600)2232973-0 |x 1179-2000 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2003 |g number:3 |g month:09 |g pages:163-167 |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1007/BF03260033 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_SPRINGER | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a GBV_ILN_120 | ||
| 912 | |a GBV_ILN_150 | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2003 |e 3 |c 09 |h 163-167 | ||
| author_variant |
v b vb f s fs m l ml e p ep |
|---|---|
| matchkey_str |
article:11792000:2003----::ptibrvrsrnfrainfualmhbaticlsrmainsihrglxydoenuevralcags |
| hierarchy_sort_str |
2003 |
| publishDate |
2003 |
| allfields |
10.1007/BF03260033 doi (DE-627)SPR035370351 (SPR)BF03260033-e DE-627 ger DE-627 rakwb eng Bonilla, Victoria verfasserin aut Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Adis Data Information BV 2003 Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 Sobrino, Francisco aut Lucas, Miguel aut Pintado, Elizabeth aut Enthalten in Molecular diagnosis & therapy [S.l.] : Springer International, 2006 7(2003), 3 vom: Sept., Seite 163-167 (DE-627)51122799X (DE-600)2232973-0 1179-2000 nnns volume:7 year:2003 number:3 month:09 pages:163-167 https://dx.doi.org/10.1007/BF03260033 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 AR 7 2003 3 09 163-167 |
| spelling |
10.1007/BF03260033 doi (DE-627)SPR035370351 (SPR)BF03260033-e DE-627 ger DE-627 rakwb eng Bonilla, Victoria verfasserin aut Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Adis Data Information BV 2003 Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 Sobrino, Francisco aut Lucas, Miguel aut Pintado, Elizabeth aut Enthalten in Molecular diagnosis & therapy [S.l.] : Springer International, 2006 7(2003), 3 vom: Sept., Seite 163-167 (DE-627)51122799X (DE-600)2232973-0 1179-2000 nnns volume:7 year:2003 number:3 month:09 pages:163-167 https://dx.doi.org/10.1007/BF03260033 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 AR 7 2003 3 09 163-167 |
| allfields_unstemmed |
10.1007/BF03260033 doi (DE-627)SPR035370351 (SPR)BF03260033-e DE-627 ger DE-627 rakwb eng Bonilla, Victoria verfasserin aut Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Adis Data Information BV 2003 Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 Sobrino, Francisco aut Lucas, Miguel aut Pintado, Elizabeth aut Enthalten in Molecular diagnosis & therapy [S.l.] : Springer International, 2006 7(2003), 3 vom: Sept., Seite 163-167 (DE-627)51122799X (DE-600)2232973-0 1179-2000 nnns volume:7 year:2003 number:3 month:09 pages:163-167 https://dx.doi.org/10.1007/BF03260033 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 AR 7 2003 3 09 163-167 |
| allfieldsGer |
10.1007/BF03260033 doi (DE-627)SPR035370351 (SPR)BF03260033-e DE-627 ger DE-627 rakwb eng Bonilla, Victoria verfasserin aut Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Adis Data Information BV 2003 Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 Sobrino, Francisco aut Lucas, Miguel aut Pintado, Elizabeth aut Enthalten in Molecular diagnosis & therapy [S.l.] : Springer International, 2006 7(2003), 3 vom: Sept., Seite 163-167 (DE-627)51122799X (DE-600)2232973-0 1179-2000 nnns volume:7 year:2003 number:3 month:09 pages:163-167 https://dx.doi.org/10.1007/BF03260033 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 AR 7 2003 3 09 163-167 |
| allfieldsSound |
10.1007/BF03260033 doi (DE-627)SPR035370351 (SPR)BF03260033-e DE-627 ger DE-627 rakwb eng Bonilla, Victoria verfasserin aut Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Adis Data Information BV 2003 Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 Sobrino, Francisco aut Lucas, Miguel aut Pintado, Elizabeth aut Enthalten in Molecular diagnosis & therapy [S.l.] : Springer International, 2006 7(2003), 3 vom: Sept., Seite 163-167 (DE-627)51122799X (DE-600)2232973-0 1179-2000 nnns volume:7 year:2003 number:3 month:09 pages:163-167 https://dx.doi.org/10.1007/BF03260033 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 AR 7 2003 3 09 163-167 |
| language |
English |
| source |
Enthalten in Molecular diagnosis & therapy 7(2003), 3 vom: Sept., Seite 163-167 volume:7 year:2003 number:3 month:09 pages:163-167 |
| sourceStr |
Enthalten in Molecular diagnosis & therapy 7(2003), 3 vom: Sept., Seite 163-167 volume:7 year:2003 number:3 month:09 pages:163-167 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Lymphoblastoid Cell Line Immortalize Cell Full Mutation Premutated Carrier Roche Diagnostics Corporation |
| isfreeaccess_bool |
false |
| container_title |
Molecular diagnosis & therapy |
| authorswithroles_txt_mv |
Bonilla, Victoria @@aut@@ Sobrino, Francisco @@aut@@ Lucas, Miguel @@aut@@ Pintado, Elizabeth @@aut@@ |
| publishDateDaySort_date |
2003-09-01T00:00:00Z |
| hierarchy_top_id |
51122799X |
| id |
SPR035370351 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR035370351</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519114445.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2003 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/BF03260033</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR035370351</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)BF03260033-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bonilla, Victoria</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Adis Data Information BV 2003</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lymphoblastoid Cell Line</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immortalize Cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Full Mutation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Premutated Carrier</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Roche Diagnostics Corporation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sobrino, Francisco</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lucas, Miguel</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pintado, Elizabeth</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Molecular diagnosis & therapy</subfield><subfield code="d">[S.l.] : Springer International, 2006</subfield><subfield code="g">7(2003), 3 vom: Sept., Seite 163-167</subfield><subfield code="w">(DE-627)51122799X</subfield><subfield code="w">(DE-600)2232973-0</subfield><subfield code="x">1179-2000</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:7</subfield><subfield code="g">year:2003</subfield><subfield code="g">number:3</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:163-167</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/BF03260033</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">7</subfield><subfield code="j">2003</subfield><subfield code="e">3</subfield><subfield code="c">09</subfield><subfield code="h">163-167</subfield></datafield></record></collection>
|
| author |
Bonilla, Victoria |
| spellingShingle |
Bonilla, Victoria misc Lymphoblastoid Cell Line misc Immortalize Cell misc Full Mutation misc Premutated Carrier misc Roche Diagnostics Corporation Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| authorStr |
Bonilla, Victoria |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)51122799X |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut |
| collection |
springer |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1179-2000 |
| topic_title |
Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation Lymphoblastoid Cell Line (dpeaa)DE-He213 Immortalize Cell (dpeaa)DE-He213 Full Mutation (dpeaa)DE-He213 Premutated Carrier (dpeaa)DE-He213 Roche Diagnostics Corporation (dpeaa)DE-He213 |
| topic |
misc Lymphoblastoid Cell Line misc Immortalize Cell misc Full Mutation misc Premutated Carrier misc Roche Diagnostics Corporation |
| topic_unstemmed |
misc Lymphoblastoid Cell Line misc Immortalize Cell misc Full Mutation misc Premutated Carrier misc Roche Diagnostics Corporation |
| topic_browse |
misc Lymphoblastoid Cell Line misc Immortalize Cell misc Full Mutation misc Premutated Carrier misc Roche Diagnostics Corporation |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| hierarchy_parent_title |
Molecular diagnosis & therapy |
| hierarchy_parent_id |
51122799X |
| hierarchy_top_title |
Molecular diagnosis & therapy |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)51122799X (DE-600)2232973-0 |
| title |
Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| ctrlnum |
(DE-627)SPR035370351 (SPR)BF03260033-e |
| title_full |
Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| author_sort |
Bonilla, Victoria |
| journal |
Molecular diagnosis & therapy |
| journalStr |
Molecular diagnosis & therapy |
| lang_code |
eng |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
2003 |
| contenttype_str_mv |
txt |
| container_start_page |
163 |
| author_browse |
Bonilla, Victoria Sobrino, Francisco Lucas, Miguel Pintado, Elizabeth |
| container_volume |
7 |
| format_se |
Elektronische Aufsätze |
| author-letter |
Bonilla, Victoria |
| doi_str_mv |
10.1007/BF03260033 |
| title_sort |
epstein-barr virus transformation of human lymphoblastoid cells from patients with fragile x syndrome induces variable changes on cgg repeats size and promoter methylation |
| title_auth |
Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| abstract |
Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. © Adis Data Information BV 2003 |
| abstractGer |
Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. © Adis Data Information BV 2003 |
| abstract_unstemmed |
Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects. © Adis Data Information BV 2003 |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_120 GBV_ILN_150 |
| container_issue |
3 |
| title_short |
Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation |
| url |
https://dx.doi.org/10.1007/BF03260033 |
| remote_bool |
true |
| author2 |
Sobrino, Francisco Lucas, Miguel Pintado, Elizabeth |
| author2Str |
Sobrino, Francisco Lucas, Miguel Pintado, Elizabeth |
| ppnlink |
51122799X |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/BF03260033 |
| up_date |
2024-07-03T14:17:27.611Z |
| _version_ |
1803567756861243392 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR035370351</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519114445.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2003 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/BF03260033</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR035370351</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)BF03260033-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bonilla, Victoria</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Epstein-Barr Virus Transformation of Human Lymphoblastoid Cells from Patients with Fragile X Syndrome Induces Variable Changes on CGG Repeats Size and Promoter Methylation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2003</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Adis Data Information BV 2003</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Background: Our understanding of fragile X syndrome can be improved by reversing the expression of the silenced fragile X mental retardation 1 (FMR1) gene in immortalized cells from these patients. Epstein-Barr virus (EBV) infection has been extensively used to transform B cells into a permanent lymphoblastoid cell line. Methods: We immortalized B lymphocytes from three different fragile X patients and one normal male. We analyzed the CGG triplet repeats and methylation status of the FMR1 and interferon (IFN)-γ promoter. We also assayed FMR1 mRNA levels by real-time PCR and FMR1 protein (FMRP) by Western blot. Results: We observed that EBV transformation may induce the instability of CGG repeats and DNA demethylation that can lead to the modification of mRNA expression. Conclusions: EBV transformation may induce variable changes in the genome that can lead to the misinterpretations of experimental data obtained from these cells. Thus, periodic testing of DNA from immortalized cells should be routinely undertaken to detect undesired effects.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lymphoblastoid Cell Line</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immortalize Cell</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Full Mutation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Premutated Carrier</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Roche Diagnostics Corporation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sobrino, Francisco</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lucas, Miguel</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pintado, Elizabeth</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Molecular diagnosis & therapy</subfield><subfield code="d">[S.l.] : Springer International, 2006</subfield><subfield code="g">7(2003), 3 vom: Sept., Seite 163-167</subfield><subfield code="w">(DE-627)51122799X</subfield><subfield code="w">(DE-600)2232973-0</subfield><subfield code="x">1179-2000</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:7</subfield><subfield code="g">year:2003</subfield><subfield code="g">number:3</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:163-167</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/BF03260033</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_120</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">7</subfield><subfield code="j">2003</subfield><subfield code="e">3</subfield><subfield code="c">09</subfield><subfield code="h">163-167</subfield></datafield></record></collection>
|
| score |
7.4005823 |