Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp

Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recor...
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Autor*in:	Haraguchi, Yuji [verfasserIn] Ohtsuki, Atsushi Oka, Takayuki Shimizu, Tatsuya

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Electrophysiology hERG channel High-throughput screening Automated patch-clamp

Anmerkung:	© Haraguchi et al. 2015

Übergeordnetes Werk:	Enthalten in: BMC pharmacology & toxicology - London : BioMed Central, 2012, 16(2015), 1 vom: 16. Dez.
Übergeordnetes Werk:	volume:16 ; year:2015 ; number:1 ; day:16 ; month:12

Links:	Volltext

DOI / URN:	10.1186/s40360-015-0042-9

Katalog-ID:	SPR036375446

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520			\|a Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing.
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allfields	10.1186/s40360-015-0042-9 doi (DE-627)SPR036375446 (SPR)s40360-015-0042-9-e DE-627 ger DE-627 rakwb eng Haraguchi, Yuji verfasserin aut Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Haraguchi et al. 2015 Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213 Ohtsuki, Atsushi aut Oka, Takayuki aut Shimizu, Tatsuya aut Enthalten in BMC pharmacology & toxicology London : BioMed Central, 2012 16(2015), 1 vom: 16. Dez. (DE-627)723899754 (DE-600)2680259-4 2050-6511 nnns volume:16 year:2015 number:1 day:16 month:12 https://dx.doi.org/10.1186/s40360-015-0042-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2015 1 16 12
spelling	10.1186/s40360-015-0042-9 doi (DE-627)SPR036375446 (SPR)s40360-015-0042-9-e DE-627 ger DE-627 rakwb eng Haraguchi, Yuji verfasserin aut Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Haraguchi et al. 2015 Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213 Ohtsuki, Atsushi aut Oka, Takayuki aut Shimizu, Tatsuya aut Enthalten in BMC pharmacology & toxicology London : BioMed Central, 2012 16(2015), 1 vom: 16. Dez. (DE-627)723899754 (DE-600)2680259-4 2050-6511 nnns volume:16 year:2015 number:1 day:16 month:12 https://dx.doi.org/10.1186/s40360-015-0042-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2015 1 16 12
allfields_unstemmed	10.1186/s40360-015-0042-9 doi (DE-627)SPR036375446 (SPR)s40360-015-0042-9-e DE-627 ger DE-627 rakwb eng Haraguchi, Yuji verfasserin aut Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Haraguchi et al. 2015 Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213 Ohtsuki, Atsushi aut Oka, Takayuki aut Shimizu, Tatsuya aut Enthalten in BMC pharmacology & toxicology London : BioMed Central, 2012 16(2015), 1 vom: 16. Dez. (DE-627)723899754 (DE-600)2680259-4 2050-6511 nnns volume:16 year:2015 number:1 day:16 month:12 https://dx.doi.org/10.1186/s40360-015-0042-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2015 1 16 12
allfieldsGer	10.1186/s40360-015-0042-9 doi (DE-627)SPR036375446 (SPR)s40360-015-0042-9-e DE-627 ger DE-627 rakwb eng Haraguchi, Yuji verfasserin aut Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Haraguchi et al. 2015 Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213 Ohtsuki, Atsushi aut Oka, Takayuki aut Shimizu, Tatsuya aut Enthalten in BMC pharmacology & toxicology London : BioMed Central, 2012 16(2015), 1 vom: 16. Dez. (DE-627)723899754 (DE-600)2680259-4 2050-6511 nnns volume:16 year:2015 number:1 day:16 month:12 https://dx.doi.org/10.1186/s40360-015-0042-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2015 1 16 12
allfieldsSound	10.1186/s40360-015-0042-9 doi (DE-627)SPR036375446 (SPR)s40360-015-0042-9-e DE-627 ger DE-627 rakwb eng Haraguchi, Yuji verfasserin aut Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Haraguchi et al. 2015 Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213 Ohtsuki, Atsushi aut Oka, Takayuki aut Shimizu, Tatsuya aut Enthalten in BMC pharmacology & toxicology London : BioMed Central, 2012 16(2015), 1 vom: 16. Dez. (DE-627)723899754 (DE-600)2680259-4 2050-6511 nnns volume:16 year:2015 number:1 day:16 month:12 https://dx.doi.org/10.1186/s40360-015-0042-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 16 2015 1 16 12
language	English
source	Enthalten in BMC pharmacology & toxicology 16(2015), 1 vom: 16. Dez. volume:16 year:2015 number:1 day:16 month:12
sourceStr	Enthalten in BMC pharmacology & toxicology 16(2015), 1 vom: 16. Dez. volume:16 year:2015 number:1 day:16 month:12
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topic_facet	Electrophysiology hERG channel High-throughput screening Automated patch-clamp
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authorswithroles_txt_mv	Haraguchi, Yuji @@aut@@ Ohtsuki, Atsushi @@aut@@ Oka, Takayuki @@aut@@ Shimizu, Tatsuya @@aut@@
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Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. 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author	Haraguchi, Yuji
spellingShingle	Haraguchi, Yuji misc Electrophysiology misc hERG channel misc High-throughput screening misc Automated patch-clamp Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
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topic_title	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp Electrophysiology (dpeaa)DE-He213 hERG channel (dpeaa)DE-He213 High-throughput screening (dpeaa)DE-He213 Automated patch-clamp (dpeaa)DE-He213
topic	misc Electrophysiology misc hERG channel misc High-throughput screening misc Automated patch-clamp
topic_unstemmed	misc Electrophysiology misc hERG channel misc High-throughput screening misc Automated patch-clamp
topic_browse	misc Electrophysiology misc hERG channel misc High-throughput screening misc Automated patch-clamp
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title	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
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title_full	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
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author_browse	Haraguchi, Yuji Ohtsuki, Atsushi Oka, Takayuki Shimizu, Tatsuya
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title_sort	electrophysiological analysis of mammalian cells expressing herg using automated 384-well-patch-clamp
title_auth	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
abstract	Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. © Haraguchi et al. 2015
abstractGer	Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. © Haraguchi et al. 2015
abstract_unstemmed	Background An in vitro electrophysiological assay system, which can assess compound effects and thus show cardiotoxicity including arrhythmia risks of test drugs, is an essential method in the field of drug development and toxicology. Methods In this study, high-throughput electrophysiological recordings of human embryonic kidney (HEK 293) cells and Chinese hamster ovary (CHO) cells stably expressing human ether-a-go-go related gene (hERG) were performed utilizing an automated 384-well-patch-clamp system, which records up to 384 cells simultaneously. hERG channel inhibition, which is closely related to a drug-induced QT prolongation and is increasing the risk of sudden cardiac death, was investigated in the high-throughput screening patch-clamp system. Results In the automated patch-clamp measurements performed here, $ K_{v} $ currents were investigated with high efficiency. Various hERG channel blockers showed concentration-dependent inhibition, the 50 % inhibitory concentrations ($ IC_{50} $) of those blockers were in good agreement with previous reports. Conclusions The high-throughput patch-clamp system has a high potential in the field of pharmacology, toxicology, and cardiac physiology, and will contribute to the acceleration of pharmaceutical drug development and drug safety testing. © Haraguchi et al. 2015
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title_short	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp
url	https://dx.doi.org/10.1186/s40360-015-0042-9
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author2	Ohtsuki, Atsushi Oka, Takayuki Shimizu, Tatsuya
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up_date	2024-07-03T17:14:47.079Z
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Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp

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