Effects of several storage media on viability and proliferation capacity of periodontal ligament cells

Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced...
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Gespeichert in:

Autor*in:	Souza, B. D. M. [verfasserIn] Garcia, L. F. R. Bortoluzzi, E. A. Felippe, W. T. Felippe, M. C. S.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Periodontal ligament fibroblasts Cell viability Cell proliferation MTT assay Storage media

Anmerkung:	© European Academy of Paediatric Dentistry 2019

Übergeordnetes Werk:	Enthalten in: European archives of paediatric dentistry - Leeds : European Academy of Paediatric Dentistry, 2006, 21(2019), 1 vom: 18. Mai, Seite 53-59
Übergeordnetes Werk:	volume:21 ; year:2019 ; number:1 ; day:18 ; month:05 ; pages:53-59

Links:	Volltext

DOI / URN:	10.1007/s40368-019-00450-8

Katalog-ID:	SPR036393088

Internformat


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245	1	0	\|a Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
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520			\|a Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results.
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700	1		\|a Garcia, L. F. R. \|0 (orcid)0000-0002-8724-0124 \|4 aut
700	1		\|a Bortoluzzi, E. A. \|4 aut
700	1		\|a Felippe, W. T. \|4 aut
700	1		\|a Felippe, M. C. S. \|4 aut
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912			\|a GBV_ILN_95
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912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
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912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
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912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
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912			\|a GBV_ILN_702
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912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
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912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2070
912			\|a GBV_ILN_2086
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2116
912			\|a GBV_ILN_2118
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912			\|a GBV_ILN_4334
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allfields	10.1007/s40368-019-00450-8 doi (DE-627)SPR036393088 (SPR)s40368-019-00450-8-e DE-627 ger DE-627 rakwb eng Souza, B. D. M. verfasserin aut Effects of several storage media on viability and proliferation capacity of periodontal ligament cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Academy of Paediatric Dentistry 2019 Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213 Garcia, L. F. R. (orcid)0000-0002-8724-0124 aut Bortoluzzi, E. A. aut Felippe, W. T. aut Felippe, M. C. S. aut Enthalten in European archives of paediatric dentistry Leeds : European Academy of Paediatric Dentistry, 2006 21(2019), 1 vom: 18. Mai, Seite 53-59 (DE-627)590957686 (DE-600)2477222-7 1996-9805 nnns volume:21 year:2019 number:1 day:18 month:05 pages:53-59 https://dx.doi.org/10.1007/s40368-019-00450-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 21 2019 1 18 05 53-59
spelling	10.1007/s40368-019-00450-8 doi (DE-627)SPR036393088 (SPR)s40368-019-00450-8-e DE-627 ger DE-627 rakwb eng Souza, B. D. M. verfasserin aut Effects of several storage media on viability and proliferation capacity of periodontal ligament cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Academy of Paediatric Dentistry 2019 Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213 Garcia, L. F. R. (orcid)0000-0002-8724-0124 aut Bortoluzzi, E. A. aut Felippe, W. T. aut Felippe, M. C. S. aut Enthalten in European archives of paediatric dentistry Leeds : European Academy of Paediatric Dentistry, 2006 21(2019), 1 vom: 18. Mai, Seite 53-59 (DE-627)590957686 (DE-600)2477222-7 1996-9805 nnns volume:21 year:2019 number:1 day:18 month:05 pages:53-59 https://dx.doi.org/10.1007/s40368-019-00450-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 21 2019 1 18 05 53-59
allfields_unstemmed	10.1007/s40368-019-00450-8 doi (DE-627)SPR036393088 (SPR)s40368-019-00450-8-e DE-627 ger DE-627 rakwb eng Souza, B. D. M. verfasserin aut Effects of several storage media on viability and proliferation capacity of periodontal ligament cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Academy of Paediatric Dentistry 2019 Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213 Garcia, L. F. R. (orcid)0000-0002-8724-0124 aut Bortoluzzi, E. A. aut Felippe, W. T. aut Felippe, M. C. S. aut Enthalten in European archives of paediatric dentistry Leeds : European Academy of Paediatric Dentistry, 2006 21(2019), 1 vom: 18. Mai, Seite 53-59 (DE-627)590957686 (DE-600)2477222-7 1996-9805 nnns volume:21 year:2019 number:1 day:18 month:05 pages:53-59 https://dx.doi.org/10.1007/s40368-019-00450-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 21 2019 1 18 05 53-59
allfieldsGer	10.1007/s40368-019-00450-8 doi (DE-627)SPR036393088 (SPR)s40368-019-00450-8-e DE-627 ger DE-627 rakwb eng Souza, B. D. M. verfasserin aut Effects of several storage media on viability and proliferation capacity of periodontal ligament cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Academy of Paediatric Dentistry 2019 Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213 Garcia, L. F. R. (orcid)0000-0002-8724-0124 aut Bortoluzzi, E. A. aut Felippe, W. T. aut Felippe, M. C. S. aut Enthalten in European archives of paediatric dentistry Leeds : European Academy of Paediatric Dentistry, 2006 21(2019), 1 vom: 18. Mai, Seite 53-59 (DE-627)590957686 (DE-600)2477222-7 1996-9805 nnns volume:21 year:2019 number:1 day:18 month:05 pages:53-59 https://dx.doi.org/10.1007/s40368-019-00450-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 21 2019 1 18 05 53-59
allfieldsSound	10.1007/s40368-019-00450-8 doi (DE-627)SPR036393088 (SPR)s40368-019-00450-8-e DE-627 ger DE-627 rakwb eng Souza, B. D. M. verfasserin aut Effects of several storage media on viability and proliferation capacity of periodontal ligament cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Academy of Paediatric Dentistry 2019 Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213 Garcia, L. F. R. (orcid)0000-0002-8724-0124 aut Bortoluzzi, E. A. aut Felippe, W. T. aut Felippe, M. C. S. aut Enthalten in European archives of paediatric dentistry Leeds : European Academy of Paediatric Dentistry, 2006 21(2019), 1 vom: 18. Mai, Seite 53-59 (DE-627)590957686 (DE-600)2477222-7 1996-9805 nnns volume:21 year:2019 number:1 day:18 month:05 pages:53-59 https://dx.doi.org/10.1007/s40368-019-00450-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 21 2019 1 18 05 53-59
language	English
source	Enthalten in European archives of paediatric dentistry 21(2019), 1 vom: 18. Mai, Seite 53-59 volume:21 year:2019 number:1 day:18 month:05 pages:53-59
sourceStr	Enthalten in European archives of paediatric dentistry 21(2019), 1 vom: 18. Mai, Seite 53-59 volume:21 year:2019 number:1 day:18 month:05 pages:53-59
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Periodontal ligament fibroblasts Cell viability Cell proliferation MTT assay Storage media
isfreeaccess_bool	false
container_title	European archives of paediatric dentistry
authorswithroles_txt_mv	Souza, B. D. M. @@aut@@ Garcia, L. F. R. @@aut@@ Bortoluzzi, E. A. @@aut@@ Felippe, W. T. @@aut@@ Felippe, M. C. S. @@aut@@
publishDateDaySort_date	2019-05-18T00:00:00Z
hierarchy_top_id	590957686
id	SPR036393088
language_de	englisch
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M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effects of several storage media on viability and proliferation capacity of periodontal ligament cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© European Academy of Paediatric Dentistry 2019</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. 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author	Souza, B. D. M.
spellingShingle	Souza, B. D. M. misc Periodontal ligament fibroblasts misc Cell viability misc Cell proliferation misc MTT assay misc Storage media Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
authorStr	Souza, B. D. M.
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topic_title	Effects of several storage media on viability and proliferation capacity of periodontal ligament cells Periodontal ligament fibroblasts (dpeaa)DE-He213 Cell viability (dpeaa)DE-He213 Cell proliferation (dpeaa)DE-He213 MTT assay (dpeaa)DE-He213 Storage media (dpeaa)DE-He213
topic	misc Periodontal ligament fibroblasts misc Cell viability misc Cell proliferation misc MTT assay misc Storage media
topic_unstemmed	misc Periodontal ligament fibroblasts misc Cell viability misc Cell proliferation misc MTT assay misc Storage media
topic_browse	misc Periodontal ligament fibroblasts misc Cell viability misc Cell proliferation misc MTT assay misc Storage media
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title	Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
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title_full	Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
author_sort	Souza, B. D. M.
journal	European archives of paediatric dentistry
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author_browse	Souza, B. D. M. Garcia, L. F. R. Bortoluzzi, E. A. Felippe, W. T. Felippe, M. C. S.
container_volume	21
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author-letter	Souza, B. D. M.
doi_str_mv	10.1007/s40368-019-00450-8
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title_sort	effects of several storage media on viability and proliferation capacity of periodontal ligament cells
title_auth	Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
abstract	Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. © European Academy of Paediatric Dentistry 2019
abstractGer	Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. © European Academy of Paediatric Dentistry 2019
abstract_unstemmed	Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. At 20 °C, HBSS had better results. © European Academy of Paediatric Dentistry 2019
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title_short	Effects of several storage media on viability and proliferation capacity of periodontal ligament cells
url	https://dx.doi.org/10.1007/s40368-019-00450-8
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author2	Garcia, L. F. R. Bortoluzzi, E. A. Felippe, W. T. Felippe, M. C. S.
author2Str	Garcia, L. F. R. Bortoluzzi, E. A. Felippe, W. T. Felippe, M. C. S.
ppnlink	590957686
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doi_str	10.1007/s40368-019-00450-8
up_date	2024-07-03T17:21:28.991Z
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D. M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effects of several storage media on viability and proliferation capacity of periodontal ligament cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© European Academy of Paediatric Dentistry 2019</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aim The purpose of this study was to investigate the effect of different storage media on viability and proliferation capacity of periodontal ligament cells. Methods Plates with periodontal ligament fibroblasts (PDLF) cells were incubated in skimmed and whole milk, recently prepared Hank’s balanced salt solution (HBSS), Save-A-Tooth system’s, coconut water, propylene glycol with 20% propolis, egg white, tap water (negative control) at 5 °C and 20 °C, for 24 h. In one of the plates of each temperature, cell viability was determined by MTT assay. In the remaining plates, the wells were filled and incubated with Minimum Essential Medium (MEM) at 37 °C for 24, 48, 72, 96, and 120 h. The proliferation capacity of PDFL cells was also evaluated by MTT assay. Data were statistically analyzed by Kruskal–Wallis, Scheffé and Mann–Whitney tests (α = 5%). Results At 5 °C, milk maintained more viable cells than other storage media immediately after exposure (0 h) and allowed greater proliferation capacity. At 20 °C, milk and HBSS had similar and allowed similar proliferation ability at 24 and 48 h. From 72 h onwards, capacity to maintain cell viability the proliferation rate of cells incubated in HBSS was superior than milk. At both temperature and experiments, Save-A-Tooth system was similar to tap water. Conclusion Milk and HBSS were more effective in maintaining cellular viability and proliferation capacity than any other storage media. At 5 °C, the most viable alternative was milk. 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Effects of several storage media on viability and proliferation capacity of periodontal ligament cells

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