Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum

Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) techniqu...
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Autor*in:	Song, Juanjuan [verfasserIn] Ye, Guoliang Qian, Zhengjiang Ye, Qing

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Cell pressure probe Hydraulic conductivity Plant water relations VIGS Water channels

Anmerkung:	© The Author(s) 2016

Übergeordnetes Werk:	Enthalten in: Botanical studies - Heidelberg : Springer, 2006, 57(2016), 1 vom: 06. Aug.
Übergeordnetes Werk:	volume:57 ; year:2016 ; number:1 ; day:06 ; month:08

Links:	Volltext

DOI / URN:	10.1186/s40529-016-0135-9

Katalog-ID:	SPR036622737

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520			\|a Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport.
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author_variant	j s js g y gy z q zq q y qy
matchkey_str	article:19993110:2016----::iuidcdlsaebaeqaoippp1iecnihbtpatae
hierarchy_sort_str	2016
publishDate	2016
allfields	10.1186/s40529-016-0135-9 doi (DE-627)SPR036622737 (SPR)s40529-016-0135-9-e DE-627 ger DE-627 rakwb eng Song, Juanjuan verfasserin aut Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213 Ye, Guoliang aut Qian, Zhengjiang aut Ye, Qing aut Enthalten in Botanical studies Heidelberg : Springer, 2006 57(2016), 1 vom: 06. Aug. (DE-627)56961791X (DE-600)2432110-2 1999-3110 nnns volume:57 year:2016 number:1 day:06 month:08 https://dx.doi.org/10.1186/s40529-016-0135-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 57 2016 1 06 08
spelling	10.1186/s40529-016-0135-9 doi (DE-627)SPR036622737 (SPR)s40529-016-0135-9-e DE-627 ger DE-627 rakwb eng Song, Juanjuan verfasserin aut Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213 Ye, Guoliang aut Qian, Zhengjiang aut Ye, Qing aut Enthalten in Botanical studies Heidelberg : Springer, 2006 57(2016), 1 vom: 06. Aug. (DE-627)56961791X (DE-600)2432110-2 1999-3110 nnns volume:57 year:2016 number:1 day:06 month:08 https://dx.doi.org/10.1186/s40529-016-0135-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 57 2016 1 06 08
allfields_unstemmed	10.1186/s40529-016-0135-9 doi (DE-627)SPR036622737 (SPR)s40529-016-0135-9-e DE-627 ger DE-627 rakwb eng Song, Juanjuan verfasserin aut Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213 Ye, Guoliang aut Qian, Zhengjiang aut Ye, Qing aut Enthalten in Botanical studies Heidelberg : Springer, 2006 57(2016), 1 vom: 06. Aug. (DE-627)56961791X (DE-600)2432110-2 1999-3110 nnns volume:57 year:2016 number:1 day:06 month:08 https://dx.doi.org/10.1186/s40529-016-0135-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 57 2016 1 06 08
allfieldsGer	10.1186/s40529-016-0135-9 doi (DE-627)SPR036622737 (SPR)s40529-016-0135-9-e DE-627 ger DE-627 rakwb eng Song, Juanjuan verfasserin aut Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213 Ye, Guoliang aut Qian, Zhengjiang aut Ye, Qing aut Enthalten in Botanical studies Heidelberg : Springer, 2006 57(2016), 1 vom: 06. Aug. (DE-627)56961791X (DE-600)2432110-2 1999-3110 nnns volume:57 year:2016 number:1 day:06 month:08 https://dx.doi.org/10.1186/s40529-016-0135-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 57 2016 1 06 08
allfieldsSound	10.1186/s40529-016-0135-9 doi (DE-627)SPR036622737 (SPR)s40529-016-0135-9-e DE-627 ger DE-627 rakwb eng Song, Juanjuan verfasserin aut Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213 Ye, Guoliang aut Qian, Zhengjiang aut Ye, Qing aut Enthalten in Botanical studies Heidelberg : Springer, 2006 57(2016), 1 vom: 06. Aug. (DE-627)56961791X (DE-600)2432110-2 1999-3110 nnns volume:57 year:2016 number:1 day:06 month:08 https://dx.doi.org/10.1186/s40529-016-0135-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 57 2016 1 06 08
language	English
source	Enthalten in Botanical studies 57(2016), 1 vom: 06. Aug. volume:57 year:2016 number:1 day:06 month:08
sourceStr	Enthalten in Botanical studies 57(2016), 1 vom: 06. Aug. volume:57 year:2016 number:1 day:06 month:08
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Cell pressure probe Hydraulic conductivity Plant water relations VIGS Water channels
isfreeaccess_bool	true
container_title	Botanical studies
authorswithroles_txt_mv	Song, Juanjuan @@aut@@ Ye, Guoliang @@aut@@ Qian, Zhengjiang @@aut@@ Ye, Qing @@aut@@
publishDateDaySort_date	2016-08-06T00:00:00Z
hierarchy_top_id	56961791X
id	SPR036622737
language_de	englisch
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In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. 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author	Song, Juanjuan
spellingShingle	Song, Juanjuan misc Cell pressure probe misc Hydraulic conductivity misc Plant water relations misc VIGS misc Water channels Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
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topic_title	Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum Cell pressure probe (dpeaa)DE-He213 Hydraulic conductivity (dpeaa)DE-He213 Plant water relations (dpeaa)DE-He213 VIGS (dpeaa)DE-He213 Water channels (dpeaa)DE-He213
topic	misc Cell pressure probe misc Hydraulic conductivity misc Plant water relations misc VIGS misc Water channels
topic_unstemmed	misc Cell pressure probe misc Hydraulic conductivity misc Plant water relations misc VIGS misc Water channels
topic_browse	misc Cell pressure probe misc Hydraulic conductivity misc Plant water relations misc VIGS misc Water channels
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title	Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
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title_full	Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
author_sort	Song, Juanjuan
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author_browse	Song, Juanjuan Ye, Guoliang Qian, Zhengjiang Ye, Qing
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author-letter	Song, Juanjuan
doi_str_mv	10.1186/s40529-016-0135-9
title_sort	virus-induced plasma membrane aquaporin pspip2;1 silencing inhibits plant water transport of pisum sativum
title_auth	Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
abstract	Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. © The Author(s) 2016
abstractGer	Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. © The Author(s) 2016
abstract_unstemmed	Background Aquaporins (AQPs) are known to facilitate water transport across cell membranes, but the role of a single AQP in regulating plant water transport, particularly in plants other than Arabidopsis remains largely unexplored. In the present study, a virus-induced gene silencing (VIGS) technique was employed to suppress the expression of a specific plasma membrane aquaporin PsPIP2;1 of Pea plants (Pisum sativum), and subsequent effects of the gene suppression on root hydraulic conductivity ($ Lp_{r} $), leaf hydraulic conductivity (Kleaf), root cell hydraulic conductivity ($ Lp_{rc} $), and leaf cell hydraulic conductivity ($ Lp_{lc} $) were investigated, using hydroponically grown Pea plants. Results Compared with control plants, VIGS-PsPIP2;1 plants displayed a significant suppression of PsPIP2;1 in both roots and leaves, while the expression of other four PIP isoforms (PsPIP1;1, PsPIP1;2, PsPIP2;2, and PsPIP2;3) that were simultaneously monitored were not altered. As a consequence, significant declines in water transport of VIGS-PsPIP2;1 plants were observed at both organ and cell levels, i.e., as compared to control plants, $ Lp_{r} $ and Kleaf were reduced by 29 %, and $ Lp_{rc} $ and $ Lp_{lc} $ were reduced by 20 and 29 %, respectively. Conclusion Our results demonstrate that PsPIP2;1 alone contributes substantially to root and leaf water transport in Pea plants, and highlight VIGS a useful tool for investigating the role of a single AQP in regulating plant water transport. © The Author(s) 2016
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title_short	Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum
url	https://dx.doi.org/10.1186/s40529-016-0135-9
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Virus-induced plasma membrane aquaporin PsPIP2;1 silencing inhibits plant water transport of Pisum sativum

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