Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin

Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Cardoso, Sueli A [verfasserIn] Oliveira, Hellen L Münchow, Eliseu A Carreño, Neftali LV Gonini Junior, Alcides Piva, Evandro

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Adhesives Dentin bonding agents Drug stability Product storage Storage of substances Tensile bond strength

Anmerkung:	© Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (

Übergeordnetes Werk:	Enthalten in: Applied Adhesion Science - Heidelberg : SpringerOpen, 2013, 2(2014), 1 vom: 24. Dez.
Übergeordnetes Werk:	volume:2 ; year:2014 ; number:1 ; day:24 ; month:12

Links:	Volltext

DOI / URN:	10.1186/s40563-014-0026-9

Katalog-ID:	SPR036667102

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520			\|a Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used.
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allfields	10.1186/s40563-014-0026-9 doi (DE-627)SPR036667102 (SPR)s40563-014-0026-9-e DE-627 ger DE-627 rakwb eng Cardoso, Sueli A verfasserin aut Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213 Oliveira, Hellen L aut Münchow, Eliseu A aut Carreño, Neftali LV aut Gonini Junior, Alcides aut Piva, Evandro aut Enthalten in Applied Adhesion Science Heidelberg : SpringerOpen, 2013 2(2014), 1 vom: 24. Dez. (DE-627)77663318X (DE-600)2750262-4 2196-4351 nnns volume:2 year:2014 number:1 day:24 month:12 https://dx.doi.org/10.1186/s40563-014-0026-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2014 1 24 12
spelling	10.1186/s40563-014-0026-9 doi (DE-627)SPR036667102 (SPR)s40563-014-0026-9-e DE-627 ger DE-627 rakwb eng Cardoso, Sueli A verfasserin aut Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213 Oliveira, Hellen L aut Münchow, Eliseu A aut Carreño, Neftali LV aut Gonini Junior, Alcides aut Piva, Evandro aut Enthalten in Applied Adhesion Science Heidelberg : SpringerOpen, 2013 2(2014), 1 vom: 24. Dez. (DE-627)77663318X (DE-600)2750262-4 2196-4351 nnns volume:2 year:2014 number:1 day:24 month:12 https://dx.doi.org/10.1186/s40563-014-0026-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2014 1 24 12
allfields_unstemmed	10.1186/s40563-014-0026-9 doi (DE-627)SPR036667102 (SPR)s40563-014-0026-9-e DE-627 ger DE-627 rakwb eng Cardoso, Sueli A verfasserin aut Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213 Oliveira, Hellen L aut Münchow, Eliseu A aut Carreño, Neftali LV aut Gonini Junior, Alcides aut Piva, Evandro aut Enthalten in Applied Adhesion Science Heidelberg : SpringerOpen, 2013 2(2014), 1 vom: 24. Dez. (DE-627)77663318X (DE-600)2750262-4 2196-4351 nnns volume:2 year:2014 number:1 day:24 month:12 https://dx.doi.org/10.1186/s40563-014-0026-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2014 1 24 12
allfieldsGer	10.1186/s40563-014-0026-9 doi (DE-627)SPR036667102 (SPR)s40563-014-0026-9-e DE-627 ger DE-627 rakwb eng Cardoso, Sueli A verfasserin aut Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213 Oliveira, Hellen L aut Münchow, Eliseu A aut Carreño, Neftali LV aut Gonini Junior, Alcides aut Piva, Evandro aut Enthalten in Applied Adhesion Science Heidelberg : SpringerOpen, 2013 2(2014), 1 vom: 24. Dez. (DE-627)77663318X (DE-600)2750262-4 2196-4351 nnns volume:2 year:2014 number:1 day:24 month:12 https://dx.doi.org/10.1186/s40563-014-0026-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2014 1 24 12
allfieldsSound	10.1186/s40563-014-0026-9 doi (DE-627)SPR036667102 (SPR)s40563-014-0026-9-e DE-627 ger DE-627 rakwb eng Cardoso, Sueli A verfasserin aut Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213 Oliveira, Hellen L aut Münchow, Eliseu A aut Carreño, Neftali LV aut Gonini Junior, Alcides aut Piva, Evandro aut Enthalten in Applied Adhesion Science Heidelberg : SpringerOpen, 2013 2(2014), 1 vom: 24. Dez. (DE-627)77663318X (DE-600)2750262-4 2196-4351 nnns volume:2 year:2014 number:1 day:24 month:12 https://dx.doi.org/10.1186/s40563-014-0026-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2014 1 24 12
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author	Cardoso, Sueli A
spellingShingle	Cardoso, Sueli A misc Adhesives misc Dentin bonding agents misc Drug stability misc Product storage misc Storage of substances misc Tensile bond strength Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
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topic_title	Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin Adhesives (dpeaa)DE-He213 Dentin bonding agents (dpeaa)DE-He213 Drug stability (dpeaa)DE-He213 Product storage (dpeaa)DE-He213 Storage of substances (dpeaa)DE-He213 Tensile bond strength (dpeaa)DE-He213
topic	misc Adhesives misc Dentin bonding agents misc Drug stability misc Product storage misc Storage of substances misc Tensile bond strength
topic_unstemmed	misc Adhesives misc Dentin bonding agents misc Drug stability misc Product storage misc Storage of substances misc Tensile bond strength
topic_browse	misc Adhesives misc Dentin bonding agents misc Drug stability misc Product storage misc Storage of substances misc Tensile bond strength
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title	Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
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title_full	Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
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author_browse	Cardoso, Sueli A Oliveira, Hellen L Münchow, Eliseu A Carreño, Neftali LV Gonini Junior, Alcides Piva, Evandro
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title_sort	effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
title_auth	Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
abstract	Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstractGer	Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
abstract_unstemmed	Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used. © Cardoso et al.; licensee Springer. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (
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title_short	Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin
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author2	Oliveira, Hellen L Münchow, Eliseu A Carreño, Neftali LV Gonini Junior, Alcides Piva, Evandro
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Self-etch adhesive systems are composed of various monomers, solvents, fillers, and initiators that make their molecular formulations quite complex. The intricate design involved in these systems has raised uncertainties regarding the long-term chemical stability of the components prior to clinical application. Therefore, this study aimed to investigate the effect of shelf-life simulation on the bond strength of self-etch adhesives to dentin. Sound human teeth samples were used and restored using one of three different adhesives: AdheSE™ (Ivoclar Vivadent), Single Bond Universal™ (3 M ESPE), or Clearfil SE Bond™ (Kuraray); Filtek Z350™ (3 M ESPE) was used as composite resin. The study (bond strength testing) was conducted in two distinct parts: (1) without shelf-life simulation of adhesives; and (2) after storing the adhesives in a climate chamber at 40°C and 50% relative humidity (shelf-life simulation). Both groups were prepared for microtensile bond strength (μTBS) testing; however, specimens from the first part of the study were evaluated after 24 h and 6 months of storage in distilled water, whereas specimens from the second part of the study were prepared and tested after 1, 2, and 3 months of shelf-life simulation of adhesives. The hybrid layer and fracture pattern of specimens were analyzed by scanning electron microscopy (SEM). Bond strength data were analyzed using Kruskal-Wallis test and Tukey’s test (α = 5%). When no shelf-life simulation was applied, Single Bond Universal increased bond strength after long-term water storage, whereas AdheSE and Clearfil SE Bond reduced bond strength to dentin. However, the bonding ability of all three adhesive systems investigated was negatively influenced by the shelf-life simulation used.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Adhesives</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dentin bonding agents</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Drug stability</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Product storage</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Storage of substances</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tensile bond strength</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Oliveira, Hellen L</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Münchow, Eliseu A</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Carreño, Neftali LV</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gonini Junior, Alcides</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Piva, Evandro</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied Adhesion Science</subfield><subfield code="d">Heidelberg : SpringerOpen, 2013</subfield><subfield code="g">2(2014), 1 vom: 24. Dez.</subfield><subfield code="w">(DE-627)77663318X</subfield><subfield code="w">(DE-600)2750262-4</subfield><subfield code="x">2196-4351</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:2</subfield><subfield code="g">year:2014</subfield><subfield code="g">number:1</subfield><subfield code="g">day:24</subfield><subfield code="g">month:12</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1186/s40563-014-0026-9</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" 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Effect of shelf-life simulation on the bond strength of self-etch adhesive systems to dentin

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