Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn
Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been assoc...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bulgarelli, Rafaela Gagetti [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© Brazilian Society of Plant Physiology 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Theoretical and experimental plant physiology - Berlin : Springer, 2014, 28(2016), 4 vom: 29. Juni, Seite 371-383 |
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| Übergeordnetes Werk: |
volume:28 ; year:2016 ; number:4 ; day:29 ; month:06 ; pages:371-383 |
| Links: |
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| DOI / URN: |
10.1007/s40626-016-0075-5 |
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SPR036933627 |
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| 520 | |a Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. | ||
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| 700 | 1 | |a Andrade, Sara Adrián L. |4 aut | |
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10.1007/s40626-016-0075-5 doi (DE-627)SPR036933627 (SPR)s40626-016-0075-5-e DE-627 ger DE-627 rakwb eng Bulgarelli, Rafaela Gagetti verfasserin aut Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Brazilian Society of Plant Physiology 2016 Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 Araujo, Pedro aut Tezotto, Tiago aut Mazzafera, Paulo aut Andrade, Sara Adrián L. aut Enthalten in Theoretical and experimental plant physiology Berlin : Springer, 2014 28(2016), 4 vom: 29. Juni, Seite 371-383 (DE-627)790227800 (DE-600)2776145-9 2197-0025 nnns volume:28 year:2016 number:4 day:29 month:06 pages:371-383 https://dx.doi.org/10.1007/s40626-016-0075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2016 4 29 06 371-383 |
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10.1007/s40626-016-0075-5 doi (DE-627)SPR036933627 (SPR)s40626-016-0075-5-e DE-627 ger DE-627 rakwb eng Bulgarelli, Rafaela Gagetti verfasserin aut Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Brazilian Society of Plant Physiology 2016 Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 Araujo, Pedro aut Tezotto, Tiago aut Mazzafera, Paulo aut Andrade, Sara Adrián L. aut Enthalten in Theoretical and experimental plant physiology Berlin : Springer, 2014 28(2016), 4 vom: 29. Juni, Seite 371-383 (DE-627)790227800 (DE-600)2776145-9 2197-0025 nnns volume:28 year:2016 number:4 day:29 month:06 pages:371-383 https://dx.doi.org/10.1007/s40626-016-0075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2016 4 29 06 371-383 |
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10.1007/s40626-016-0075-5 doi (DE-627)SPR036933627 (SPR)s40626-016-0075-5-e DE-627 ger DE-627 rakwb eng Bulgarelli, Rafaela Gagetti verfasserin aut Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Brazilian Society of Plant Physiology 2016 Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 Araujo, Pedro aut Tezotto, Tiago aut Mazzafera, Paulo aut Andrade, Sara Adrián L. aut Enthalten in Theoretical and experimental plant physiology Berlin : Springer, 2014 28(2016), 4 vom: 29. Juni, Seite 371-383 (DE-627)790227800 (DE-600)2776145-9 2197-0025 nnns volume:28 year:2016 number:4 day:29 month:06 pages:371-383 https://dx.doi.org/10.1007/s40626-016-0075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2016 4 29 06 371-383 |
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10.1007/s40626-016-0075-5 doi (DE-627)SPR036933627 (SPR)s40626-016-0075-5-e DE-627 ger DE-627 rakwb eng Bulgarelli, Rafaela Gagetti verfasserin aut Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Brazilian Society of Plant Physiology 2016 Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 Araujo, Pedro aut Tezotto, Tiago aut Mazzafera, Paulo aut Andrade, Sara Adrián L. aut Enthalten in Theoretical and experimental plant physiology Berlin : Springer, 2014 28(2016), 4 vom: 29. Juni, Seite 371-383 (DE-627)790227800 (DE-600)2776145-9 2197-0025 nnns volume:28 year:2016 number:4 day:29 month:06 pages:371-383 https://dx.doi.org/10.1007/s40626-016-0075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2016 4 29 06 371-383 |
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10.1007/s40626-016-0075-5 doi (DE-627)SPR036933627 (SPR)s40626-016-0075-5-e DE-627 ger DE-627 rakwb eng Bulgarelli, Rafaela Gagetti verfasserin aut Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Brazilian Society of Plant Physiology 2016 Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 Araujo, Pedro aut Tezotto, Tiago aut Mazzafera, Paulo aut Andrade, Sara Adrián L. aut Enthalten in Theoretical and experimental plant physiology Berlin : Springer, 2014 28(2016), 4 vom: 29. Juni, Seite 371-383 (DE-627)790227800 (DE-600)2776145-9 2197-0025 nnns volume:28 year:2016 number:4 day:29 month:06 pages:371-383 https://dx.doi.org/10.1007/s40626-016-0075-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2016 4 29 06 371-383 |
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Bulgarelli, Rafaela Gagetti @@aut@@ Araujo, Pedro @@aut@@ Tezotto, Tiago @@aut@@ Mazzafera, Paulo @@aut@@ Andrade, Sara Adrián L. @@aut@@ |
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Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. 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| author |
Bulgarelli, Rafaela Gagetti |
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Bulgarelli, Rafaela Gagetti misc Metal homeostasis misc Oxidative stress misc Nutrient solution Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn |
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Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn Metal homeostasis (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Nutrient solution (dpeaa)DE-He213 |
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misc Metal homeostasis misc Oxidative stress misc Nutrient solution |
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Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn |
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Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn |
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Bulgarelli, Rafaela Gagetti |
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Theoretical and experimental plant physiology |
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Bulgarelli, Rafaela Gagetti Araujo, Pedro Tezotto, Tiago Mazzafera, Paulo Andrade, Sara Adrián L. |
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Bulgarelli, Rafaela Gagetti |
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10.1007/s40626-016-0075-5 |
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expression of metallothionein genes in coffee leaves in response to the absence or excess of cu and zn |
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Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn |
| abstract |
Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. © Brazilian Society of Plant Physiology 2016 |
| abstractGer |
Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. © Brazilian Society of Plant Physiology 2016 |
| abstract_unstemmed |
Abstract Copper (Cu) and zinc (Zn) are plant nutrients; however, intracellular concentration must be kept within the sufficiency limits in order to adequately maintain metabolic functions. Like other organisms, plants have metallothioneins (MTs), which are cysteine-rich peptides that have been associated with metal homeostasis and detoxification because of their capacity to bind divalent metals. Nonetheless, the physiological roles of MTs are not completely understood and much is still unknown concerning their characterization in many higher plant species. In this study, MT expression was analyzed in leaves of Coffea arabica plants exposed to the absence or high levels of Cu and Zn in a nutrient solution, aiming to investigate the role of MTs in the maintenance of Cu and Zn homeostasis and in detoxification of excessive levels of these nutrients. At the same time, the studied MT sequences underwent phylogenetic analysis, showing that in C. arabica three of the studied MTs showed characteristics of three of the four different groups of plant MTs. It was observed that CaMT4 and CaMT15 belonged to group I but also presented characteristics of group II. CaMT3 showed protein characteristics of MT group II, and CaMT8 was positioned in type III MT clade. The expression levels of genes CaMT3, CaMT4, CaMT8, and CaMT15 were evaluated in leaves. The results indicated that MT type I, II, and III studied in C. arabica leaves are probably involved in Cu homeostasis and detoxification. However, further studies are needed to identify other MTs in coffee plants and specifically those involved in response to Zn, since the evaluation here showed a low response to excessive concentrations of this metal. © Brazilian Society of Plant Physiology 2016 |
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Expression of metallothionein genes in coffee leaves in response to the absence or excess of Cu and Zn |
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https://dx.doi.org/10.1007/s40626-016-0075-5 |
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Araujo, Pedro Tezotto, Tiago Mazzafera, Paulo Andrade, Sara Adrián L. |
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Araujo, Pedro Tezotto, Tiago Mazzafera, Paulo Andrade, Sara Adrián L. |
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10.1007/s40626-016-0075-5 |
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2024-07-03T20:26:39.268Z |
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| score |
7.4032135 |