Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan
Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tseng, Chih-Sian [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Anmerkung: |
© China Medical University 2014 |
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| Übergeordnetes Werk: |
Enthalten in: Biomedicine - [Taichung] : China Medical University, 2011, 4(2014), 3 vom: 13. Aug. |
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| Übergeordnetes Werk: |
volume:4 ; year:2014 ; number:3 ; day:13 ; month:08 |
| Links: |
|---|
| DOI / URN: |
10.7603/s40681-014-0020-3 |
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| Katalog-ID: |
SPR037284223 |
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| 245 | 1 | 0 | |a Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
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| 520 | |a Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. | ||
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10.7603/s40681-014-0020-3 doi (DE-627)SPR037284223 (SPR)s40681-014-0020-3-e DE-627 ger DE-627 rakwb eng Tseng, Chih-Sian verfasserin aut Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © China Medical University 2014 Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 Yen, Yu-Chieh aut Chang, Chao-Chin aut Hsu, Yuan-Man aut Enthalten in Biomedicine [Taichung] : China Medical University, 2011 4(2014), 3 vom: 13. Aug. (DE-627)684564637 (DE-600)2648006-2 2211-8039 nnns volume:4 year:2014 number:3 day:13 month:08 https://dx.doi.org/10.7603/s40681-014-0020-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 3 13 08 |
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10.7603/s40681-014-0020-3 doi (DE-627)SPR037284223 (SPR)s40681-014-0020-3-e DE-627 ger DE-627 rakwb eng Tseng, Chih-Sian verfasserin aut Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © China Medical University 2014 Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 Yen, Yu-Chieh aut Chang, Chao-Chin aut Hsu, Yuan-Man aut Enthalten in Biomedicine [Taichung] : China Medical University, 2011 4(2014), 3 vom: 13. Aug. (DE-627)684564637 (DE-600)2648006-2 2211-8039 nnns volume:4 year:2014 number:3 day:13 month:08 https://dx.doi.org/10.7603/s40681-014-0020-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 3 13 08 |
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10.7603/s40681-014-0020-3 doi (DE-627)SPR037284223 (SPR)s40681-014-0020-3-e DE-627 ger DE-627 rakwb eng Tseng, Chih-Sian verfasserin aut Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © China Medical University 2014 Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 Yen, Yu-Chieh aut Chang, Chao-Chin aut Hsu, Yuan-Man aut Enthalten in Biomedicine [Taichung] : China Medical University, 2011 4(2014), 3 vom: 13. Aug. (DE-627)684564637 (DE-600)2648006-2 2211-8039 nnns volume:4 year:2014 number:3 day:13 month:08 https://dx.doi.org/10.7603/s40681-014-0020-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 3 13 08 |
| allfieldsGer |
10.7603/s40681-014-0020-3 doi (DE-627)SPR037284223 (SPR)s40681-014-0020-3-e DE-627 ger DE-627 rakwb eng Tseng, Chih-Sian verfasserin aut Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © China Medical University 2014 Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 Yen, Yu-Chieh aut Chang, Chao-Chin aut Hsu, Yuan-Man aut Enthalten in Biomedicine [Taichung] : China Medical University, 2011 4(2014), 3 vom: 13. Aug. (DE-627)684564637 (DE-600)2648006-2 2211-8039 nnns volume:4 year:2014 number:3 day:13 month:08 https://dx.doi.org/10.7603/s40681-014-0020-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 3 13 08 |
| allfieldsSound |
10.7603/s40681-014-0020-3 doi (DE-627)SPR037284223 (SPR)s40681-014-0020-3-e DE-627 ger DE-627 rakwb eng Tseng, Chih-Sian verfasserin aut Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © China Medical University 2014 Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 Yen, Yu-Chieh aut Chang, Chao-Chin aut Hsu, Yuan-Man aut Enthalten in Biomedicine [Taichung] : China Medical University, 2011 4(2014), 3 vom: 13. Aug. (DE-627)684564637 (DE-600)2648006-2 2211-8039 nnns volume:4 year:2014 number:3 day:13 month:08 https://dx.doi.org/10.7603/s40681-014-0020-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 3 13 08 |
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Enthalten in Biomedicine 4(2014), 3 vom: 13. Aug. volume:4 year:2014 number:3 day:13 month:08 |
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Tseng, Chih-Sian @@aut@@ Yen, Yu-Chieh @@aut@@ Chang, Chao-Chin @@aut@@ Hsu, Yuan-Man @@aut@@ |
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Tseng, Chih-Sian |
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Tseng, Chih-Sian misc Class 1 integrons misc Gene cassette misc Promoter Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
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Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan Class 1 integrons (dpeaa)DE-He213 Gene cassette (dpeaa)DE-He213 Promoter (dpeaa)DE-He213 |
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Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
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Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
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polymorphism of gene cassette promoter variants of class 1 integron harbored in s. choleraesuis and typhimurium isolated from taiwan |
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Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
| abstract |
Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. © China Medical University 2014 |
| abstractGer |
Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. © China Medical University 2014 |
| abstract_unstemmed |
Abstract Integrons, mobile genetic units, capture and incorporate antibiotic resistance gene cassette by site-specific recombination. Class 1 integrons are widespread and associated with dispersion of antibiotic resistance among Gram-negative bacteria. The expression of gene cassette in Class 1 can vary, based on the Pc promoter but seldom from another promoter hiding downstream of Pc, called $ P_{2} $. To probe distribution and prevalence of gene cassette promoter variants, we analyzed 169 S. Choleraesuis and 191 S. Typhimurium isolates from humans and animals, finding 95.27% occurrence of integrin among S. Choleraesuis, 83.25% among S. Typhimurium. PCR-RFLP analysis identified four promoters (PcS+$ P_{2} $, $ PcW_{TGN-10} $+$ P_{2} $, PcH1+$ P_{2} $, and $ Pc_{WTGN-10} $+$ P_{2} $-GGG) in said integron-positive isolates; major types in S. Choleraesuis and S. Typhimurium were PcS+$ P_{2} $ and $ Pc_{WTGN-10} $+$ P_{2} $, respectively. Likewise, β-galactosidase assay rated promoter strength of variants by transcriptional fusion constructs to show extended -10 promoter (TGn/-10 promoter) in Pc and three-nucleotide insertion (GGG) between -35 and -10 region of $ P_{2} $ improving promoter strength of gene cassette. © China Medical University 2014 |
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Polymorphism of gene cassette promoter variants of class 1 integron harbored in S. Choleraesuis and Typhimurium isolated from Taiwan |
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| score |
7.3997393 |