PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence
Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was develo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chen, Li-Shyang [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Anmerkung: |
© Sociedade Brasileira de Fitopatologia 2015 |
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| Übergeordnetes Werk: |
Enthalten in: Tropical plant pathology - Brasília, 2008, 40(2015), 1 vom: Feb., Seite 65-69 |
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| Übergeordnetes Werk: |
volume:40 ; year:2015 ; number:1 ; month:02 ; pages:65-69 |
| Links: |
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| DOI / URN: |
10.1007/s40858-015-0009-9 |
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| Katalog-ID: |
SPR037934678 |
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| 100 | 1 | |a Chen, Li-Shyang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
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| 520 | |a Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. | ||
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| 650 | 4 | |a Molecular diagnosis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Liu, Chii-Dong |4 aut | |
| 700 | 1 | |a Tsay, Jwu-Guh |4 aut | |
| 700 | 1 | |a Chen, Ruey-Shayang |4 aut | |
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10.1007/s40858-015-0009-9 doi (DE-627)SPR037934678 (SPR)s40858-015-0009-9-e DE-627 ger DE-627 rakwb eng Chen, Li-Shyang verfasserin aut PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sociedade Brasileira de Fitopatologia 2015 Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 Liu, Chii-Dong aut Tsay, Jwu-Guh aut Chen, Ruey-Shayang aut Enthalten in Tropical plant pathology Brasília, 2008 40(2015), 1 vom: Feb., Seite 65-69 (DE-627)610937081 (DE-600)2519866-X 1983-2052 nnns volume:40 year:2015 number:1 month:02 pages:65-69 https://dx.doi.org/10.1007/s40858-015-0009-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2015 1 02 65-69 |
| spelling |
10.1007/s40858-015-0009-9 doi (DE-627)SPR037934678 (SPR)s40858-015-0009-9-e DE-627 ger DE-627 rakwb eng Chen, Li-Shyang verfasserin aut PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sociedade Brasileira de Fitopatologia 2015 Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 Liu, Chii-Dong aut Tsay, Jwu-Guh aut Chen, Ruey-Shayang aut Enthalten in Tropical plant pathology Brasília, 2008 40(2015), 1 vom: Feb., Seite 65-69 (DE-627)610937081 (DE-600)2519866-X 1983-2052 nnns volume:40 year:2015 number:1 month:02 pages:65-69 https://dx.doi.org/10.1007/s40858-015-0009-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2015 1 02 65-69 |
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10.1007/s40858-015-0009-9 doi (DE-627)SPR037934678 (SPR)s40858-015-0009-9-e DE-627 ger DE-627 rakwb eng Chen, Li-Shyang verfasserin aut PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sociedade Brasileira de Fitopatologia 2015 Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 Liu, Chii-Dong aut Tsay, Jwu-Guh aut Chen, Ruey-Shayang aut Enthalten in Tropical plant pathology Brasília, 2008 40(2015), 1 vom: Feb., Seite 65-69 (DE-627)610937081 (DE-600)2519866-X 1983-2052 nnns volume:40 year:2015 number:1 month:02 pages:65-69 https://dx.doi.org/10.1007/s40858-015-0009-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2015 1 02 65-69 |
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10.1007/s40858-015-0009-9 doi (DE-627)SPR037934678 (SPR)s40858-015-0009-9-e DE-627 ger DE-627 rakwb eng Chen, Li-Shyang verfasserin aut PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sociedade Brasileira de Fitopatologia 2015 Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 Liu, Chii-Dong aut Tsay, Jwu-Guh aut Chen, Ruey-Shayang aut Enthalten in Tropical plant pathology Brasília, 2008 40(2015), 1 vom: Feb., Seite 65-69 (DE-627)610937081 (DE-600)2519866-X 1983-2052 nnns volume:40 year:2015 number:1 month:02 pages:65-69 https://dx.doi.org/10.1007/s40858-015-0009-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2015 1 02 65-69 |
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10.1007/s40858-015-0009-9 doi (DE-627)SPR037934678 (SPR)s40858-015-0009-9-e DE-627 ger DE-627 rakwb eng Chen, Li-Shyang verfasserin aut PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Sociedade Brasileira de Fitopatologia 2015 Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 Liu, Chii-Dong aut Tsay, Jwu-Guh aut Chen, Ruey-Shayang aut Enthalten in Tropical plant pathology Brasília, 2008 40(2015), 1 vom: Feb., Seite 65-69 (DE-627)610937081 (DE-600)2519866-X 1983-2052 nnns volume:40 year:2015 number:1 month:02 pages:65-69 https://dx.doi.org/10.1007/s40858-015-0009-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_252 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2015 1 02 65-69 |
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Chen, Li-Shyang @@aut@@ Liu, Chii-Dong @@aut@@ Tsay, Jwu-Guh @@aut@@ Chen, Ruey-Shayang @@aut@@ |
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Chen, Li-Shyang |
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Chen, Li-Shyang misc Culmicolous smut misc Molecular diagnosis PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
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PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence Culmicolous smut (dpeaa)DE-He213 Molecular diagnosis (dpeaa)DE-He213 |
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PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
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PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
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pcr-mediated detection of sporisorium scitamineum in sugarcane based on the be mating-type gene sequence |
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PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
| abstract |
Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. © Sociedade Brasileira de Fitopatologia 2015 |
| abstractGer |
Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. © Sociedade Brasileira de Fitopatologia 2015 |
| abstract_unstemmed |
Abstract Culmicolous smut caused by Sporisorium scitamineum is a main sugarcane disease, occurring in all sugarcane-producing areas of the world. The disease develops sori with whip-shaped axes corresponding to columellae and causes economic yield loss. In this study, a PCR-mediated assay was developed based on the bE mating-type gene sequence for the early detection of S. scitamineum in sugarcane tissues. Primer pair CLS 320 F/CLS 320R was designed and tested for the detection of S. scitamineum in sugarcane tissue. It was successful to rapidly detect S. scitamineum in DNA extracted from as few as 10 mg of diseased sugarcane tissue within one hour. The detection limit of the primer pair was tested and shown to amplify DNA at the pg level. The amplification products could be detected in DNA extracted from stem, whip-shaped axes and leaves of diseased plants. This high specificity assay can be applied to the detection of S. scitamineum in infected sugarcane tissues. Results presented here indicate that the primer pair CLS 320 F/CLS 320R is efficient in identifying S. scitamineum and accurately and rapidly detecting the disease caused by this pathogen in the field. © Sociedade Brasileira de Fitopatologia 2015 |
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PCR-Mediated Detection of Sporisorium scitamineum in Sugarcane based on the bE Mating-Type Gene Sequence |
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https://dx.doi.org/10.1007/s40858-015-0009-9 |
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Liu, Chii-Dong Tsay, Jwu-Guh Chen, Ruey-Shayang |
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2024-07-03T15:15:05.991Z |
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