Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up

Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of oste...
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Autor*in:	Kämmerer, P. W. [verfasserIn] Thiem, D. G. E. Alshihri, A. Wittstock, G. H. Bader, R. Al-Nawas, B. Klein, M. O.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	Bioengineering Biomechanics Dental implant materials Implant healing Cell biology Osteoblast Stress analysis

Anmerkung:	© The Author(s). 2017

Übergeordnetes Werk:	Enthalten in: International journal of implant dentistry - Berlin : Springer, 2015, 3(2017), 1 vom: 31. Mai
Übergeordnetes Werk:	volume:3 ; year:2017 ; number:1 ; day:31 ; month:05

Links:	Volltext

DOI / URN:	10.1186/s40729-017-0085-3

Katalog-ID:	SPR038080486

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520			\|a Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment.
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allfields	10.1186/s40729-017-0085-3 doi (DE-627)SPR038080486 (SPR)s40729-017-0085-3-e DE-627 ger DE-627 rakwb eng Kämmerer, P. W. verfasserin aut Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213 Thiem, D. G. E. aut Alshihri, A. aut Wittstock, G. H. aut Bader, R. aut Al-Nawas, B. aut Klein, M. O. aut Enthalten in International journal of implant dentistry Berlin : Springer, 2015 3(2017), 1 vom: 31. Mai (DE-627)84409952X (DE-600)2842869-9 2198-4034 nnns volume:3 year:2017 number:1 day:31 month:05 https://dx.doi.org/10.1186/s40729-017-0085-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2017 1 31 05
spelling	10.1186/s40729-017-0085-3 doi (DE-627)SPR038080486 (SPR)s40729-017-0085-3-e DE-627 ger DE-627 rakwb eng Kämmerer, P. W. verfasserin aut Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213 Thiem, D. G. E. aut Alshihri, A. aut Wittstock, G. H. aut Bader, R. aut Al-Nawas, B. aut Klein, M. O. aut Enthalten in International journal of implant dentistry Berlin : Springer, 2015 3(2017), 1 vom: 31. Mai (DE-627)84409952X (DE-600)2842869-9 2198-4034 nnns volume:3 year:2017 number:1 day:31 month:05 https://dx.doi.org/10.1186/s40729-017-0085-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2017 1 31 05
allfields_unstemmed	10.1186/s40729-017-0085-3 doi (DE-627)SPR038080486 (SPR)s40729-017-0085-3-e DE-627 ger DE-627 rakwb eng Kämmerer, P. W. verfasserin aut Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213 Thiem, D. G. E. aut Alshihri, A. aut Wittstock, G. H. aut Bader, R. aut Al-Nawas, B. aut Klein, M. O. aut Enthalten in International journal of implant dentistry Berlin : Springer, 2015 3(2017), 1 vom: 31. Mai (DE-627)84409952X (DE-600)2842869-9 2198-4034 nnns volume:3 year:2017 number:1 day:31 month:05 https://dx.doi.org/10.1186/s40729-017-0085-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2017 1 31 05
allfieldsGer	10.1186/s40729-017-0085-3 doi (DE-627)SPR038080486 (SPR)s40729-017-0085-3-e DE-627 ger DE-627 rakwb eng Kämmerer, P. W. verfasserin aut Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213 Thiem, D. G. E. aut Alshihri, A. aut Wittstock, G. H. aut Bader, R. aut Al-Nawas, B. aut Klein, M. O. aut Enthalten in International journal of implant dentistry Berlin : Springer, 2015 3(2017), 1 vom: 31. Mai (DE-627)84409952X (DE-600)2842869-9 2198-4034 nnns volume:3 year:2017 number:1 day:31 month:05 https://dx.doi.org/10.1186/s40729-017-0085-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2017 1 31 05
allfieldsSound	10.1186/s40729-017-0085-3 doi (DE-627)SPR038080486 (SPR)s40729-017-0085-3-e DE-627 ger DE-627 rakwb eng Kämmerer, P. W. verfasserin aut Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s). 2017 Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213 Thiem, D. G. E. aut Alshihri, A. aut Wittstock, G. H. aut Bader, R. aut Al-Nawas, B. aut Klein, M. O. aut Enthalten in International journal of implant dentistry Berlin : Springer, 2015 3(2017), 1 vom: 31. Mai (DE-627)84409952X (DE-600)2842869-9 2198-4034 nnns volume:3 year:2017 number:1 day:31 month:05 https://dx.doi.org/10.1186/s40729-017-0085-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2017 1 31 05
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source	Enthalten in International journal of implant dentistry 3(2017), 1 vom: 31. Mai volume:3 year:2017 number:1 day:31 month:05
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author	Kämmerer, P. W.
spellingShingle	Kämmerer, P. W. misc Bioengineering misc Biomechanics misc Dental implant materials misc Implant healing misc Cell biology misc Osteoblast misc Stress analysis Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
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topic_title	Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up Bioengineering (dpeaa)DE-He213 Biomechanics (dpeaa)DE-He213 Dental implant materials (dpeaa)DE-He213 Implant healing (dpeaa)DE-He213 Cell biology (dpeaa)DE-He213 Osteoblast (dpeaa)DE-He213 Stress analysis (dpeaa)DE-He213
topic	misc Bioengineering misc Biomechanics misc Dental implant materials misc Implant healing misc Cell biology misc Osteoblast misc Stress analysis
topic_unstemmed	misc Bioengineering misc Biomechanics misc Dental implant materials misc Implant healing misc Cell biology misc Osteoblast misc Stress analysis
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title	Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
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title_full	Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
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title_sort	cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
title_auth	Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
abstract	Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. © The Author(s). 2017
abstractGer	Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. © The Author(s). 2017
abstract_unstemmed	Background Mechanostimuli of different cells can affect a wide array of cellular and inter-cellular biological processes responsible for dental implant healing. The purpose of this in vitro study was to establish a new test model to create a reproducible flow-induced fluid shear stress (FSS) of osteoblast cells on implant surfaces. Methods As FSS effects on osteoblasts are detectable at 10 dyn/$ cm^{2} $, a custom-made flow chamber was created. Computer-aided verification of circulation processes was performed. In order to verify FSS effects, cells were analysed via light and fluorescence microscopy. Results Utilising computer-aided simulations, the underside of the upper plate was considered to have optimal conditions for cell culturing. At this site, a flow-induced orientation of osteoblast cell clusters and an altered cell morphology with cellular elongation and alteration of actin fibres in the fluid flow direction was detected. Conclusions FSS simulation using this novel flow chamber might mimic the peri-implant situation in the phase of loaded implant healing. With this FSS flow chamber, osteoblast cells’ sensitivity to FSS was verified in the form of morphological changes and cell re-clustering towards the direction of the flow. Different shear forces can be created simultaneously in a single experiment. © The Author(s). 2017
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title_short	Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
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