Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications
Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Datta, Pallab [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Anmerkung: |
© Indian Institute of Science 2019 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of the Indian Institute of Science - [New Delhi] : Springer India, 1914, 99(2019), 3 vom: 21. Aug., Seite 329-337 |
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| Übergeordnetes Werk: |
volume:99 ; year:2019 ; number:3 ; day:21 ; month:08 ; pages:329-337 |
| Links: |
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| DOI / URN: |
10.1007/s41745-019-00115-x |
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| Katalog-ID: |
SPR038294230 |
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| 520 | |a Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. | ||
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| 650 | 4 | |a Laser engraving |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rolling |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bone scaffold |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a MG-63 |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Dhara, Santanu |4 aut | |
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10.1007/s41745-019-00115-x doi (DE-627)SPR038294230 (SPR)s41745-019-00115-x-e DE-627 ger DE-627 rakwb eng Datta, Pallab verfasserin aut Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Institute of Science 2019 Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 Dhara, Santanu aut Enthalten in Journal of the Indian Institute of Science [New Delhi] : Springer India, 1914 99(2019), 3 vom: 21. Aug., Seite 329-337 (DE-627)38292441X (DE-600)2139553-6 0019-4964 nnns volume:99 year:2019 number:3 day:21 month:08 pages:329-337 https://dx.doi.org/10.1007/s41745-019-00115-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 99 2019 3 21 08 329-337 |
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10.1007/s41745-019-00115-x doi (DE-627)SPR038294230 (SPR)s41745-019-00115-x-e DE-627 ger DE-627 rakwb eng Datta, Pallab verfasserin aut Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Institute of Science 2019 Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 Dhara, Santanu aut Enthalten in Journal of the Indian Institute of Science [New Delhi] : Springer India, 1914 99(2019), 3 vom: 21. Aug., Seite 329-337 (DE-627)38292441X (DE-600)2139553-6 0019-4964 nnns volume:99 year:2019 number:3 day:21 month:08 pages:329-337 https://dx.doi.org/10.1007/s41745-019-00115-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 99 2019 3 21 08 329-337 |
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10.1007/s41745-019-00115-x doi (DE-627)SPR038294230 (SPR)s41745-019-00115-x-e DE-627 ger DE-627 rakwb eng Datta, Pallab verfasserin aut Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Institute of Science 2019 Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 Dhara, Santanu aut Enthalten in Journal of the Indian Institute of Science [New Delhi] : Springer India, 1914 99(2019), 3 vom: 21. Aug., Seite 329-337 (DE-627)38292441X (DE-600)2139553-6 0019-4964 nnns volume:99 year:2019 number:3 day:21 month:08 pages:329-337 https://dx.doi.org/10.1007/s41745-019-00115-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 99 2019 3 21 08 329-337 |
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10.1007/s41745-019-00115-x doi (DE-627)SPR038294230 (SPR)s41745-019-00115-x-e DE-627 ger DE-627 rakwb eng Datta, Pallab verfasserin aut Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Institute of Science 2019 Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 Dhara, Santanu aut Enthalten in Journal of the Indian Institute of Science [New Delhi] : Springer India, 1914 99(2019), 3 vom: 21. Aug., Seite 329-337 (DE-627)38292441X (DE-600)2139553-6 0019-4964 nnns volume:99 year:2019 number:3 day:21 month:08 pages:329-337 https://dx.doi.org/10.1007/s41745-019-00115-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 99 2019 3 21 08 329-337 |
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10.1007/s41745-019-00115-x doi (DE-627)SPR038294230 (SPR)s41745-019-00115-x-e DE-627 ger DE-627 rakwb eng Datta, Pallab verfasserin aut Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Indian Institute of Science 2019 Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 Dhara, Santanu aut Enthalten in Journal of the Indian Institute of Science [New Delhi] : Springer India, 1914 99(2019), 3 vom: 21. Aug., Seite 329-337 (DE-627)38292441X (DE-600)2139553-6 0019-4964 nnns volume:99 year:2019 number:3 day:21 month:08 pages:329-337 https://dx.doi.org/10.1007/s41745-019-00115-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 99 2019 3 21 08 329-337 |
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Enthalten in Journal of the Indian Institute of Science 99(2019), 3 vom: 21. Aug., Seite 329-337 volume:99 year:2019 number:3 day:21 month:08 pages:329-337 |
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Enthalten in Journal of the Indian Institute of Science 99(2019), 3 vom: 21. Aug., Seite 329-337 volume:99 year:2019 number:3 day:21 month:08 pages:329-337 |
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Electrospinning 3D scaffold Macro-pores Laser engraving Rolling Bone scaffold MG-63 |
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Journal of the Indian Institute of Science |
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Datta, Pallab @@aut@@ Dhara, Santanu @@aut@@ |
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However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. 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Datta, Pallab |
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Datta, Pallab misc Electrospinning misc 3D scaffold misc Macro-pores misc Laser engraving misc Rolling misc Bone scaffold misc MG-63 Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications |
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Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications Electrospinning (dpeaa)DE-He213 3D scaffold (dpeaa)DE-He213 Macro-pores (dpeaa)DE-He213 Laser engraving (dpeaa)DE-He213 Rolling (dpeaa)DE-He213 Bone scaffold (dpeaa)DE-He213 MG-63 (dpeaa)DE-He213 |
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Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications |
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| title_sort |
engineering porosity in electrospun nanofiber sheets by laser engraving: a strategy to fabricate 3d scaffolds for bone graft applications |
| title_auth |
Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications |
| abstract |
Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. © Indian Institute of Science 2019 |
| abstractGer |
Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. © Indian Institute of Science 2019 |
| abstract_unstemmed |
Abstract Nanofiber features in a scaffold provide favorable niche for cellular attachment, proliferation, and differentiation propelling their interest in tissue engineering. However, the inability of seeded cells to infiltrate inside 3D structures of electrospun nanofibers has remained a persistent bottleneck for their greater applicability. In the present work, an approach to address this problem is presented. Macro-pores are designed in common graphic software created by a laser-engraving machine on electrospun nanofiber sheets composed of a bioinspired material-N-methylene phosphonic chitosan for facilitating cellular infiltration into 3D scaffold. Effect of laser pulse energy and pulse per inch on pore morphology are investigated and FTIR spectrum is examined to preclude the degradation of material due to laser-engraving process. Furthermore, the micro-fabricated nanofiber sheets with multi-scalar porosity are rolled up to form a 3D scaffold as graft through biomimetic approach for bone-tissue engineering applications. Culture of MG-63 cells on rolled up nanofiber sheets containing laser-engraved macro-porous 3D scaffolds demonstrated no cytotoxicity induced by the scaffolds from MTT assay, while cellular migration into the sheets was evident from scanning electron microscopy. It is concluded that combined micro-fabrication-rolling approach may be simple, rapid way to design 3D bone grafts based on 2D electrospun nanofiber sheet of natural/semi-synthetic polymers for better osteoconductivity. © Indian Institute of Science 2019 |
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Engineering Porosity in Electrospun Nanofiber Sheets by Laser Engraving: A Strategy to Fabricate 3D Scaffolds for Bone Graft Applications |
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