The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans

Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its in...
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Autor*in:	Kovács, Attila L. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Sample preparation Fixation Staining Dehydration Embedding

Anmerkung:	© The Author(s) 2015

Übergeordnetes Werk:	Enthalten in: Biophysics reports - Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015, 1(2015), 2 vom: Okt., Seite 99-105
Übergeordnetes Werk:	volume:1 ; year:2015 ; number:2 ; month:10 ; pages:99-105

Links:	Volltext

DOI / URN:	10.1007/s41048-015-0014-z

Katalog-ID:	SPR038311739

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allfields	10.1007/s41048-015-0014-z doi (DE-627)SPR038311739 (SPR)s41048-015-0014-z-e DE-627 ger DE-627 rakwb eng Kovács, Attila L. verfasserin aut The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2015 Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213 Enthalten in Biophysics reports Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015 1(2015), 2 vom: Okt., Seite 99-105 (DE-627)844073350 (DE-600)2842777-4 2364-3420 nnns volume:1 year:2015 number:2 month:10 pages:99-105 https://dx.doi.org/10.1007/s41048-015-0014-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2015 2 10 99-105
spelling	10.1007/s41048-015-0014-z doi (DE-627)SPR038311739 (SPR)s41048-015-0014-z-e DE-627 ger DE-627 rakwb eng Kovács, Attila L. verfasserin aut The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2015 Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213 Enthalten in Biophysics reports Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015 1(2015), 2 vom: Okt., Seite 99-105 (DE-627)844073350 (DE-600)2842777-4 2364-3420 nnns volume:1 year:2015 number:2 month:10 pages:99-105 https://dx.doi.org/10.1007/s41048-015-0014-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2015 2 10 99-105
allfields_unstemmed	10.1007/s41048-015-0014-z doi (DE-627)SPR038311739 (SPR)s41048-015-0014-z-e DE-627 ger DE-627 rakwb eng Kovács, Attila L. verfasserin aut The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2015 Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213 Enthalten in Biophysics reports Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015 1(2015), 2 vom: Okt., Seite 99-105 (DE-627)844073350 (DE-600)2842777-4 2364-3420 nnns volume:1 year:2015 number:2 month:10 pages:99-105 https://dx.doi.org/10.1007/s41048-015-0014-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2015 2 10 99-105
allfieldsGer	10.1007/s41048-015-0014-z doi (DE-627)SPR038311739 (SPR)s41048-015-0014-z-e DE-627 ger DE-627 rakwb eng Kovács, Attila L. verfasserin aut The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2015 Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213 Enthalten in Biophysics reports Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015 1(2015), 2 vom: Okt., Seite 99-105 (DE-627)844073350 (DE-600)2842777-4 2364-3420 nnns volume:1 year:2015 number:2 month:10 pages:99-105 https://dx.doi.org/10.1007/s41048-015-0014-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2015 2 10 99-105
allfieldsSound	10.1007/s41048-015-0014-z doi (DE-627)SPR038311739 (SPR)s41048-015-0014-z-e DE-627 ger DE-627 rakwb eng Kovács, Attila L. verfasserin aut The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2015 Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213 Enthalten in Biophysics reports Beijing : Institute of Biophysics, Chinese Academy of Sciences, 2015 1(2015), 2 vom: Okt., Seite 99-105 (DE-627)844073350 (DE-600)2842777-4 2364-3420 nnns volume:1 year:2015 number:2 month:10 pages:99-105 https://dx.doi.org/10.1007/s41048-015-0014-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 1 2015 2 10 99-105
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author	Kovács, Attila L.
spellingShingle	Kovács, Attila L. misc Sample preparation misc Fixation misc Staining misc Dehydration misc Embedding The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans
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topic_title	The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans Sample preparation (dpeaa)DE-He213 Fixation (dpeaa)DE-He213 Staining (dpeaa)DE-He213 Dehydration (dpeaa)DE-He213 Embedding (dpeaa)DE-He213
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title	The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans
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title_full	The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans
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title_sort	application of traditional transmission electron microscopy for autophagy research in caenorhabditis elegans
title_auth	The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans
abstract	Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. © The Author(s) 2015
abstractGer	Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. © The Author(s) 2015
abstract_unstemmed	Abstract Traditional ultrastructural characterization of autophagic processes remains an important approach to be used in parallel with molecular genetics, light microscopy, and other methods. The special nature of Caenorhabditis elegans as an object for transmission electron microscopy makes its introduction into autophagy research a challenging task. The basis of the protocol to prepare C. elegans samples for autophagy studies was worked out around the turn of the millennium and has been used since then in my laboratory with some modifications. The method described here enables the user to prepare samples for systematic morphologic as well as morphometric investigations to characterize autophagy with a high but still realistic investment of effort. © The Author(s) 2015
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title_short	The application of traditional transmission electron microscopy for autophagy research in Caenorhabditis elegans
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