Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses

Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors....
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Chen, Qian [verfasserIn] Zheng, Limin Zhong, Panpan Jia, Dongsheng Liu, Yuyan Mao, Qianzhuo Wei, Taiyun

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Actin-based tubule motility Pns11 tubule Gelsolin Leafhopper

Anmerkung:	© The Author(s) 2019

Übergeordnetes Werk:	Enthalten in: Phytopathology research - [London] : BioMed Central, 2019, 1(2019), 1 vom: 23. Mai
Übergeordnetes Werk:	volume:1 ; year:2019 ; number:1 ; day:23 ; month:05

Links:	Volltext

DOI / URN:	10.1186/s42483-019-0025-7

Katalog-ID:	SPR038603780

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520			\|a Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus.
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allfields	10.1186/s42483-019-0025-7 doi (DE-627)SPR038603780 (SPR)s42483-019-0025-7-e DE-627 ger DE-627 rakwb eng Chen, Qian verfasserin aut Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213 Zheng, Limin aut Zhong, Panpan aut Jia, Dongsheng aut Liu, Yuyan aut Mao, Qianzhuo aut Wei, Taiyun aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 23. Mai (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:23 month:05 https://dx.doi.org/10.1186/s42483-019-0025-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 23 05
spelling	10.1186/s42483-019-0025-7 doi (DE-627)SPR038603780 (SPR)s42483-019-0025-7-e DE-627 ger DE-627 rakwb eng Chen, Qian verfasserin aut Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213 Zheng, Limin aut Zhong, Panpan aut Jia, Dongsheng aut Liu, Yuyan aut Mao, Qianzhuo aut Wei, Taiyun aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 23. Mai (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:23 month:05 https://dx.doi.org/10.1186/s42483-019-0025-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 23 05
allfields_unstemmed	10.1186/s42483-019-0025-7 doi (DE-627)SPR038603780 (SPR)s42483-019-0025-7-e DE-627 ger DE-627 rakwb eng Chen, Qian verfasserin aut Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213 Zheng, Limin aut Zhong, Panpan aut Jia, Dongsheng aut Liu, Yuyan aut Mao, Qianzhuo aut Wei, Taiyun aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 23. Mai (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:23 month:05 https://dx.doi.org/10.1186/s42483-019-0025-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 23 05
allfieldsGer	10.1186/s42483-019-0025-7 doi (DE-627)SPR038603780 (SPR)s42483-019-0025-7-e DE-627 ger DE-627 rakwb eng Chen, Qian verfasserin aut Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213 Zheng, Limin aut Zhong, Panpan aut Jia, Dongsheng aut Liu, Yuyan aut Mao, Qianzhuo aut Wei, Taiyun aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 23. Mai (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:23 month:05 https://dx.doi.org/10.1186/s42483-019-0025-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 23 05
allfieldsSound	10.1186/s42483-019-0025-7 doi (DE-627)SPR038603780 (SPR)s42483-019-0025-7-e DE-627 ger DE-627 rakwb eng Chen, Qian verfasserin aut Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213 Zheng, Limin aut Zhong, Panpan aut Jia, Dongsheng aut Liu, Yuyan aut Mao, Qianzhuo aut Wei, Taiyun aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 23. Mai (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:23 month:05 https://dx.doi.org/10.1186/s42483-019-0025-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 23 05
language	English
source	Enthalten in Phytopathology research 1(2019), 1 vom: 23. Mai volume:1 year:2019 number:1 day:23 month:05
sourceStr	Enthalten in Phytopathology research 1(2019), 1 vom: 23. Mai volume:1 year:2019 number:1 day:23 month:05
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Actin-based tubule motility Pns11 tubule Gelsolin Leafhopper
isfreeaccess_bool	true
container_title	Phytopathology research
authorswithroles_txt_mv	Chen, Qian @@aut@@ Zheng, Limin @@aut@@ Zhong, Panpan @@aut@@ Jia, Dongsheng @@aut@@ Liu, Yuyan @@aut@@ Mao, Qianzhuo @@aut@@ Wei, Taiyun @@aut@@
publishDateDaySort_date	2019-05-23T00:00:00Z
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author	Chen, Qian
spellingShingle	Chen, Qian misc Actin-based tubule motility misc Pns11 tubule misc Gelsolin misc Leafhopper Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
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topic_title	Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses Actin-based tubule motility (dpeaa)DE-He213 Pns11 tubule (dpeaa)DE-He213 Gelsolin (dpeaa)DE-He213 Leafhopper (dpeaa)DE-He213
topic	misc Actin-based tubule motility misc Pns11 tubule misc Gelsolin misc Leafhopper
topic_unstemmed	misc Actin-based tubule motility misc Pns11 tubule misc Gelsolin misc Leafhopper
topic_browse	misc Actin-based tubule motility misc Pns11 tubule misc Gelsolin misc Leafhopper
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title	Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
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title_full	Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
author_sort	Chen, Qian
journal	Phytopathology research
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lang_code	eng
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author_browse	Chen, Qian Zheng, Limin Zhong, Panpan Jia, Dongsheng Liu, Yuyan Mao, Qianzhuo Wei, Taiyun
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author-letter	Chen, Qian
doi_str_mv	10.1186/s42483-019-0025-7
title_sort	gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
title_auth	Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
abstract	Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. © The Author(s) 2019
abstractGer	Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. © The Author(s) 2019
abstract_unstemmed	Abstract Most plant reoviruses encode a type of nonstructural protein that assembles tubular structures to package virions for viral spread in planthopper or leafhopper vectors. These tubules are propelled by actin filaments and facilitate viruses to overcome transmission barriers in insect vectors. This is known as actin-based tubule motility (ABTM), in which insect proteins, especially actin-associated proteins participate. To better understand the insect components that play a role in the ABTM, the proteins interacting with tubule protein Pns11 of the Rice gall dwarf virus (RGDV) in the leafhopper vector were investigated. We found that gelsolin, an actin-modulating protein, interacted with Pns11 in the yeast-two-hybrid system and Sf9 cells. The interaction and co-localization of gelsolin and Pns11 were also verified in cultured cells and insect bodies of the leafhopper vector. Further, the expression of gelsolin was up-regulated by the RGDV infection both in cultured cells and insects. The knockdown of the gelsolin gene triggered by RNA interference increased viral accumulation, thus increasing the viruliferous rates of the leafhopper vector. This negative association of gelsolin with Pns11 and virus infection revealed that gelsolin negatively affected the ability of the virus to spread by interacting with Pns11 tubules, finally acting to negatively regulate RGDV infection. The results of this study indicate that ABTM is negatively regulated by insects in the coevolution of the insect vector and virus. © The Author(s) 2019
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700
container_issue	1
title_short	Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses
url	https://dx.doi.org/10.1186/s42483-019-0025-7
remote_bool	true
author2	Zheng, Limin Zhong, Panpan Jia, Dongsheng Liu, Yuyan Mao, Qianzhuo Wei, Taiyun
author2Str	Zheng, Limin Zhong, Panpan Jia, Dongsheng Liu, Yuyan Mao, Qianzhuo Wei, Taiyun
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up_date	2024-07-03T19:04:55.124Z
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Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses

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