Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4

Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fei, Shulang [verfasserIn] Czislowski, Elizabeth Fletcher, Stephen Peters, Jonathan Batley, Jacqueline Aitken, Elizabeth Mitter, Neena

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Banana R1 TR4 Inoculation Small RNA miR156 miR159 miR166

Anmerkung:	© The Author(s) 2019

Übergeordnetes Werk:	Enthalten in: Phytopathology research - [London] : BioMed Central, 2019, 1(2019), 1 vom: 25. Juni
Übergeordnetes Werk:	volume:1 ; year:2019 ; number:1 ; day:25 ; month:06

Links:	Volltext

DOI / URN:	10.1186/s42483-019-0029-3

Katalog-ID:	SPR038603829

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520			\|a Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement.
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allfields	10.1186/s42483-019-0029-3 doi (DE-627)SPR038603829 (SPR)s42483-019-0029-3-e DE-627 ger DE-627 rakwb eng Fei, Shulang verfasserin aut Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213 Czislowski, Elizabeth aut Fletcher, Stephen aut Peters, Jonathan aut Batley, Jacqueline aut Aitken, Elizabeth aut Mitter, Neena aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 25. Juni (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:25 month:06 https://dx.doi.org/10.1186/s42483-019-0029-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 25 06
spelling	10.1186/s42483-019-0029-3 doi (DE-627)SPR038603829 (SPR)s42483-019-0029-3-e DE-627 ger DE-627 rakwb eng Fei, Shulang verfasserin aut Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213 Czislowski, Elizabeth aut Fletcher, Stephen aut Peters, Jonathan aut Batley, Jacqueline aut Aitken, Elizabeth aut Mitter, Neena aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 25. Juni (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:25 month:06 https://dx.doi.org/10.1186/s42483-019-0029-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 25 06
allfields_unstemmed	10.1186/s42483-019-0029-3 doi (DE-627)SPR038603829 (SPR)s42483-019-0029-3-e DE-627 ger DE-627 rakwb eng Fei, Shulang verfasserin aut Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213 Czislowski, Elizabeth aut Fletcher, Stephen aut Peters, Jonathan aut Batley, Jacqueline aut Aitken, Elizabeth aut Mitter, Neena aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 25. Juni (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:25 month:06 https://dx.doi.org/10.1186/s42483-019-0029-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 25 06
allfieldsGer	10.1186/s42483-019-0029-3 doi (DE-627)SPR038603829 (SPR)s42483-019-0029-3-e DE-627 ger DE-627 rakwb eng Fei, Shulang verfasserin aut Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213 Czislowski, Elizabeth aut Fletcher, Stephen aut Peters, Jonathan aut Batley, Jacqueline aut Aitken, Elizabeth aut Mitter, Neena aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 25. Juni (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:25 month:06 https://dx.doi.org/10.1186/s42483-019-0029-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 25 06
allfieldsSound	10.1186/s42483-019-0029-3 doi (DE-627)SPR038603829 (SPR)s42483-019-0029-3-e DE-627 ger DE-627 rakwb eng Fei, Shulang verfasserin aut Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2019 Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213 Czislowski, Elizabeth aut Fletcher, Stephen aut Peters, Jonathan aut Batley, Jacqueline aut Aitken, Elizabeth aut Mitter, Neena aut Enthalten in Phytopathology research [London] : BioMed Central, 2019 1(2019), 1 vom: 25. Juni (DE-627)104821978X (DE-600)2960641-X 2524-4167 nnns volume:1 year:2019 number:1 day:25 month:06 https://dx.doi.org/10.1186/s42483-019-0029-3 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 1 2019 1 25 06
language	English
source	Enthalten in Phytopathology research 1(2019), 1 vom: 25. Juni volume:1 year:2019 number:1 day:25 month:06
sourceStr	Enthalten in Phytopathology research 1(2019), 1 vom: 25. Juni volume:1 year:2019 number:1 day:25 month:06
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institution	findex.gbv.de
topic_facet	Banana R1 TR4 Inoculation Small RNA miR156 miR159 miR166
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container_title	Phytopathology research
authorswithroles_txt_mv	Fei, Shulang @@aut@@ Czislowski, Elizabeth @@aut@@ Fletcher, Stephen @@aut@@ Peters, Jonathan @@aut@@ Batley, Jacqueline @@aut@@ Aitken, Elizabeth @@aut@@ Mitter, Neena @@aut@@
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author	Fei, Shulang
spellingShingle	Fei, Shulang misc Banana misc R1 misc TR4 misc Inoculation misc Small RNA misc miR156 misc miR159 misc miR166 Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4
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topic_title	Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4 Banana (dpeaa)DE-He213 R1 (dpeaa)DE-He213 TR4 (dpeaa)DE-He213 Inoculation (dpeaa)DE-He213 Small RNA (dpeaa)DE-He213 miR156 (dpeaa)DE-He213 miR159 (dpeaa)DE-He213 miR166 (dpeaa)DE-He213
topic	misc Banana misc R1 misc TR4 misc Inoculation misc Small RNA misc miR156 misc miR159 misc miR166
topic_unstemmed	misc Banana misc R1 misc TR4 misc Inoculation misc Small RNA misc miR156 misc miR159 misc miR166
topic_browse	misc Banana misc R1 misc TR4 misc Inoculation misc Small RNA misc miR156 misc miR159 misc miR166
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title	Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4
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title_full	Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4
author_sort	Fei, Shulang
journal	Phytopathology research
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author_browse	Fei, Shulang Czislowski, Elizabeth Fletcher, Stephen Peters, Jonathan Batley, Jacqueline Aitken, Elizabeth Mitter, Neena
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title_sort	small rna profiling of cavendish banana roots inoculated with fusarium oxysporum f. sp. cubense race 1 and tropical race 4
title_auth	Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4
abstract	Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. © The Author(s) 2019
abstractGer	Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. © The Author(s) 2019
abstract_unstemmed	Abstract Fusarium wilt, caused by the soil-borne fungal pathogen, Fusarium oxysporum f. sp. cubense (Foc), is considered as one of the most threatening diseases of banana. The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. The findings will pave way for future investigations of the defence mechanism and potential approaches of resistance improvement. © The Author(s) 2019
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700
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title_short	Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4
url	https://dx.doi.org/10.1186/s42483-019-0029-3
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author2	Czislowski, Elizabeth Fletcher, Stephen Peters, Jonathan Batley, Jacqueline Aitken, Elizabeth Mitter, Neena
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up_date	2024-07-03T19:04:56.029Z
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The Cavendish variety, resistant to Foc race 1 (R1), is susceptible to tropical race 4 (TR4), an aggressive race of the pathogen which is of increasing concern worldwide. Previous studies have revealed that plant small RNAs (sRNAs) play crucial roles in the host response to pathogen infection. To investigate the roles of sRNAs involved in the interaction of the banana-Foc pathosystem, small RNA profiles of Cavendish banana roots inoculated with Foc TR4 and Foc R1 were obtained and analyzed in the present study using Next-Generation Sequencing (NGS) technology. A total of 112 discrete mature microRNAs (miRNAs) belonging to 22 known miRNA families were found across all constructed sRNA libraries. The expression of miR166, miR159 and miR156 was up-regulated in TR4-inoculated samples as compared to mock-inoculated samples, while the expression of these miRNAs was approximately the same in R1-inoculated and mock-inoculated samples. Consistent with the sequencing data, qRT-PCR results demonstrated up-regulation of these miRNAs and down-regulation of their target genes in TR4-inoculated samples, but not in R1-inoculated samples. Considering Cavendish banana is resistant to R1 and susceptible to TR4, it is possible that these sRNAs and their target genes are involved in particular plant defence pathways such as salicylic acid-based defence. 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Small RNA profiling of Cavendish banana roots inoculated with Fusarium oxysporum f. sp. cubense race 1 and tropical race 4

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