Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles
Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The bi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
de Araújo Júnior, Raimundo Fernandes [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© Maj Institute of Pharmacology, Polish Academy of Sciences 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Pharmacological reports - Heidelberg : Springer Nature, 1998, 69(2016), 1 vom: 21. Sept., Seite 119-129 |
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| Übergeordnetes Werk: |
volume:69 ; year:2016 ; number:1 ; day:21 ; month:09 ; pages:119-129 |
| Links: |
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| DOI / URN: |
10.1016/j.pharep.2016.09.017 |
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| Katalog-ID: |
SPR038898497 |
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| 520 | |a Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. | ||
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| 650 | 4 | |a Cancer cell apoptosis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Anti-inflammatory activity |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Peripheral analgesia |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a de Araújo, Aurigena Antunes |4 aut | |
| 700 | 1 | |a Pessoa, Jonas Bispo |4 aut | |
| 700 | 1 | |a Freire Neto, Franscisco Paulo |4 aut | |
| 700 | 1 | |a da Silva, Gisele Ribeiro |4 aut | |
| 700 | 1 | |a Leitão Oliveira, Ana Luiza C. S. |4 aut | |
| 700 | 1 | |a de Carvalho, Thaís Gomes |4 aut | |
| 700 | 1 | |a Silva, Heloiza F. O. |4 aut | |
| 700 | 1 | |a Eugênio, Mateus |4 aut | |
| 700 | 1 | |a Sant’Anna, Celso |4 aut | |
| 700 | 1 | |a Gasparotto, Luiz H. S. |4 aut | |
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10.1016/j.pharep.2016.09.017 doi (DE-627)SPR038898497 (SPR)j.pharep.2016.09.017-e DE-627 ger DE-627 rakwb eng de Araújo Júnior, Raimundo Fernandes verfasserin aut Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. Gold nanoparticles (dpeaa)DE-He213 Cancer cell apoptosis (dpeaa)DE-He213 Anti-inflammatory activity (dpeaa)DE-He213 Peripheral analgesia (dpeaa)DE-He213 de Araújo, Aurigena Antunes aut Pessoa, Jonas Bispo aut Freire Neto, Franscisco Paulo aut da Silva, Gisele Ribeiro aut Leitão Oliveira, Ana Luiza C. S. aut de Carvalho, Thaís Gomes aut Silva, Heloiza F. O. aut Eugênio, Mateus aut Sant’Anna, Celso aut Gasparotto, Luiz H. S. aut Enthalten in Pharmacological reports Heidelberg : Springer Nature, 1998 69(2016), 1 vom: 21. Sept., Seite 119-129 (DE-627)375964215 (DE-600)2129019-2 2299-5684 nnns volume:69 year:2016 number:1 day:21 month:09 pages:119-129 https://dx.doi.org/10.1016/j.pharep.2016.09.017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 69 2016 1 21 09 119-129 |
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10.1016/j.pharep.2016.09.017 doi (DE-627)SPR038898497 (SPR)j.pharep.2016.09.017-e DE-627 ger DE-627 rakwb eng de Araújo Júnior, Raimundo Fernandes verfasserin aut Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. Gold nanoparticles (dpeaa)DE-He213 Cancer cell apoptosis (dpeaa)DE-He213 Anti-inflammatory activity (dpeaa)DE-He213 Peripheral analgesia (dpeaa)DE-He213 de Araújo, Aurigena Antunes aut Pessoa, Jonas Bispo aut Freire Neto, Franscisco Paulo aut da Silva, Gisele Ribeiro aut Leitão Oliveira, Ana Luiza C. S. aut de Carvalho, Thaís Gomes aut Silva, Heloiza F. O. aut Eugênio, Mateus aut Sant’Anna, Celso aut Gasparotto, Luiz H. S. aut Enthalten in Pharmacological reports Heidelberg : Springer Nature, 1998 69(2016), 1 vom: 21. Sept., Seite 119-129 (DE-627)375964215 (DE-600)2129019-2 2299-5684 nnns volume:69 year:2016 number:1 day:21 month:09 pages:119-129 https://dx.doi.org/10.1016/j.pharep.2016.09.017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 69 2016 1 21 09 119-129 |
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10.1016/j.pharep.2016.09.017 doi (DE-627)SPR038898497 (SPR)j.pharep.2016.09.017-e DE-627 ger DE-627 rakwb eng de Araújo Júnior, Raimundo Fernandes verfasserin aut Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. Gold nanoparticles (dpeaa)DE-He213 Cancer cell apoptosis (dpeaa)DE-He213 Anti-inflammatory activity (dpeaa)DE-He213 Peripheral analgesia (dpeaa)DE-He213 de Araújo, Aurigena Antunes aut Pessoa, Jonas Bispo aut Freire Neto, Franscisco Paulo aut da Silva, Gisele Ribeiro aut Leitão Oliveira, Ana Luiza C. S. aut de Carvalho, Thaís Gomes aut Silva, Heloiza F. O. aut Eugênio, Mateus aut Sant’Anna, Celso aut Gasparotto, Luiz H. S. aut Enthalten in Pharmacological reports Heidelberg : Springer Nature, 1998 69(2016), 1 vom: 21. Sept., Seite 119-129 (DE-627)375964215 (DE-600)2129019-2 2299-5684 nnns volume:69 year:2016 number:1 day:21 month:09 pages:119-129 https://dx.doi.org/10.1016/j.pharep.2016.09.017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 69 2016 1 21 09 119-129 |
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10.1016/j.pharep.2016.09.017 doi (DE-627)SPR038898497 (SPR)j.pharep.2016.09.017-e DE-627 ger DE-627 rakwb eng de Araújo Júnior, Raimundo Fernandes verfasserin aut Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. Gold nanoparticles (dpeaa)DE-He213 Cancer cell apoptosis (dpeaa)DE-He213 Anti-inflammatory activity (dpeaa)DE-He213 Peripheral analgesia (dpeaa)DE-He213 de Araújo, Aurigena Antunes aut Pessoa, Jonas Bispo aut Freire Neto, Franscisco Paulo aut da Silva, Gisele Ribeiro aut Leitão Oliveira, Ana Luiza C. S. aut de Carvalho, Thaís Gomes aut Silva, Heloiza F. O. aut Eugênio, Mateus aut Sant’Anna, Celso aut Gasparotto, Luiz H. S. aut Enthalten in Pharmacological reports Heidelberg : Springer Nature, 1998 69(2016), 1 vom: 21. Sept., Seite 119-129 (DE-627)375964215 (DE-600)2129019-2 2299-5684 nnns volume:69 year:2016 number:1 day:21 month:09 pages:119-129 https://dx.doi.org/10.1016/j.pharep.2016.09.017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 69 2016 1 21 09 119-129 |
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10.1016/j.pharep.2016.09.017 doi (DE-627)SPR038898497 (SPR)j.pharep.2016.09.017-e DE-627 ger DE-627 rakwb eng de Araújo Júnior, Raimundo Fernandes verfasserin aut Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. Gold nanoparticles (dpeaa)DE-He213 Cancer cell apoptosis (dpeaa)DE-He213 Anti-inflammatory activity (dpeaa)DE-He213 Peripheral analgesia (dpeaa)DE-He213 de Araújo, Aurigena Antunes aut Pessoa, Jonas Bispo aut Freire Neto, Franscisco Paulo aut da Silva, Gisele Ribeiro aut Leitão Oliveira, Ana Luiza C. S. aut de Carvalho, Thaís Gomes aut Silva, Heloiza F. O. aut Eugênio, Mateus aut Sant’Anna, Celso aut Gasparotto, Luiz H. S. aut Enthalten in Pharmacological reports Heidelberg : Springer Nature, 1998 69(2016), 1 vom: 21. Sept., Seite 119-129 (DE-627)375964215 (DE-600)2129019-2 2299-5684 nnns volume:69 year:2016 number:1 day:21 month:09 pages:119-129 https://dx.doi.org/10.1016/j.pharep.2016.09.017 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2098 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 69 2016 1 21 09 119-129 |
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Enthalten in Pharmacological reports 69(2016), 1 vom: 21. Sept., Seite 119-129 volume:69 year:2016 number:1 day:21 month:09 pages:119-129 |
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de Araújo Júnior, Raimundo Fernandes @@aut@@ de Araújo, Aurigena Antunes @@aut@@ Pessoa, Jonas Bispo @@aut@@ Freire Neto, Franscisco Paulo @@aut@@ da Silva, Gisele Ribeiro @@aut@@ Leitão Oliveira, Ana Luiza C. S. @@aut@@ de Carvalho, Thaís Gomes @@aut@@ Silva, Heloiza F. O. @@aut@@ Eugênio, Mateus @@aut@@ Sant’Anna, Celso @@aut@@ Gasparotto, Luiz H. S. @@aut@@ |
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2016-09-21T00:00:00Z |
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However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. 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de Araújo Júnior, Raimundo Fernandes |
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de Araújo Júnior, Raimundo Fernandes |
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de Araújo Júnior, Raimundo Fernandes de Araújo, Aurigena Antunes Pessoa, Jonas Bispo Freire Neto, Franscisco Paulo da Silva, Gisele Ribeiro Leitão Oliveira, Ana Luiza C. S. de Carvalho, Thaís Gomes Silva, Heloiza F. O. Eugênio, Mateus Sant’Anna, Celso Gasparotto, Luiz H. S. |
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de Araújo Júnior, Raimundo Fernandes |
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anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles |
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Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles |
| abstract |
Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 |
| abstractGer |
Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 |
| abstract_unstemmed |
Background Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied. Methods GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV–vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their anti-inflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV–vis and histopathological analysis, respectively. Results Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 μg/mL to 80 μg/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 μg/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 μg/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs. Conclusion The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. © Maj Institute of Pharmacology, Polish Academy of Sciences 2016 |
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Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles |
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de Araújo, Aurigena Antunes Pessoa, Jonas Bispo Freire Neto, Franscisco Paulo da Silva, Gisele Ribeiro Leitão Oliveira, Ana Luiza C. S. de Carvalho, Thaís Gomes Silva, Heloiza F. O. Eugênio, Mateus Sant’Anna, Celso Gasparotto, Luiz H. S. |
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de Araújo, Aurigena Antunes Pessoa, Jonas Bispo Freire Neto, Franscisco Paulo da Silva, Gisele Ribeiro Leitão Oliveira, Ana Luiza C. S. de Carvalho, Thaís Gomes Silva, Heloiza F. O. Eugênio, Mateus Sant’Anna, Celso Gasparotto, Luiz H. S. |
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10.1016/j.pharep.2016.09.017 |
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| score |
7.399787 |