Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities

Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throu...
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Autor*in:	Coleine, Claudia [verfasserIn] Stajich, Jason E. [verfasserIn] Pombubpa, Nuttapon [verfasserIn] Zucconi, Laura [verfasserIn] Onofri, Silvano [verfasserIn] Selbmann, Laura [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Antarctica Cryptoendolithic communities Bacteria Fungi Metabarcoding Sampling effort

Übergeordnetes Werk:	Enthalten in: Polar biology - Berlin : Springer, 1982, 43(2020), 3 vom: 17. Feb., Seite 225-235
Übergeordnetes Werk:	volume:43 ; year:2020 ; number:3 ; day:17 ; month:02 ; pages:225-235

Links:	Volltext

DOI / URN:	10.1007/s00300-020-02625-2

Katalog-ID:	SPR039040380

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520			\|a Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities.
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700	1		\|a Onofri, Silvano \|e verfasserin \|4 aut
700	1		\|a Selbmann, Laura \|e verfasserin \|4 aut
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matchkey_str	article:14322056:2020----::apigtaeisosesirbadvriyfnacicyt
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allfields	10.1007/s00300-020-02625-2 doi (DE-627)SPR039040380 (SPR)s00300-020-02625-2-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Coleine, Claudia verfasserin aut Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities. Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213 Stajich, Jason E. verfasserin aut Pombubpa, Nuttapon verfasserin aut Zucconi, Laura verfasserin aut Onofri, Silvano verfasserin aut Selbmann, Laura verfasserin aut Enthalten in Polar biology Berlin : Springer, 1982 43(2020), 3 vom: 17. Feb., Seite 225-235 (DE-627)271175427 (DE-600)1478942-5 1432-2056 nnns volume:43 year:2020 number:3 day:17 month:02 pages:225-235 https://dx.doi.org/10.1007/s00300-020-02625-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 43 2020 3 17 02 225-235
spelling	10.1007/s00300-020-02625-2 doi (DE-627)SPR039040380 (SPR)s00300-020-02625-2-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Coleine, Claudia verfasserin aut Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities. Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213 Stajich, Jason E. verfasserin aut Pombubpa, Nuttapon verfasserin aut Zucconi, Laura verfasserin aut Onofri, Silvano verfasserin aut Selbmann, Laura verfasserin aut Enthalten in Polar biology Berlin : Springer, 1982 43(2020), 3 vom: 17. Feb., Seite 225-235 (DE-627)271175427 (DE-600)1478942-5 1432-2056 nnns volume:43 year:2020 number:3 day:17 month:02 pages:225-235 https://dx.doi.org/10.1007/s00300-020-02625-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 43 2020 3 17 02 225-235
allfields_unstemmed	10.1007/s00300-020-02625-2 doi (DE-627)SPR039040380 (SPR)s00300-020-02625-2-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Coleine, Claudia verfasserin aut Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities. Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213 Stajich, Jason E. verfasserin aut Pombubpa, Nuttapon verfasserin aut Zucconi, Laura verfasserin aut Onofri, Silvano verfasserin aut Selbmann, Laura verfasserin aut Enthalten in Polar biology Berlin : Springer, 1982 43(2020), 3 vom: 17. Feb., Seite 225-235 (DE-627)271175427 (DE-600)1478942-5 1432-2056 nnns volume:43 year:2020 number:3 day:17 month:02 pages:225-235 https://dx.doi.org/10.1007/s00300-020-02625-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 43 2020 3 17 02 225-235
allfieldsGer	10.1007/s00300-020-02625-2 doi (DE-627)SPR039040380 (SPR)s00300-020-02625-2-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Coleine, Claudia verfasserin aut Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities. Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213 Stajich, Jason E. verfasserin aut Pombubpa, Nuttapon verfasserin aut Zucconi, Laura verfasserin aut Onofri, Silvano verfasserin aut Selbmann, Laura verfasserin aut Enthalten in Polar biology Berlin : Springer, 1982 43(2020), 3 vom: 17. Feb., Seite 225-235 (DE-627)271175427 (DE-600)1478942-5 1432-2056 nnns volume:43 year:2020 number:3 day:17 month:02 pages:225-235 https://dx.doi.org/10.1007/s00300-020-02625-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 43 2020 3 17 02 225-235
allfieldsSound	10.1007/s00300-020-02625-2 doi (DE-627)SPR039040380 (SPR)s00300-020-02625-2-e DE-627 ger DE-627 rakwb eng 570 ASE 42.00 bkl Coleine, Claudia verfasserin aut Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities. Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213 Stajich, Jason E. verfasserin aut Pombubpa, Nuttapon verfasserin aut Zucconi, Laura verfasserin aut Onofri, Silvano verfasserin aut Selbmann, Laura verfasserin aut Enthalten in Polar biology Berlin : Springer, 1982 43(2020), 3 vom: 17. Feb., Seite 225-235 (DE-627)271175427 (DE-600)1478942-5 1432-2056 nnns volume:43 year:2020 number:3 day:17 month:02 pages:225-235 https://dx.doi.org/10.1007/s00300-020-02625-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 42.00 ASE AR 43 2020 3 17 02 225-235
language	English
source	Enthalten in Polar biology 43(2020), 3 vom: 17. Feb., Seite 225-235 volume:43 year:2020 number:3 day:17 month:02 pages:225-235
sourceStr	Enthalten in Polar biology 43(2020), 3 vom: 17. Feb., Seite 225-235 volume:43 year:2020 number:3 day:17 month:02 pages:225-235
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Antarctica Cryptoendolithic communities Bacteria Fungi Metabarcoding Sampling effort
dewey-raw	570
isfreeaccess_bool	false
container_title	Polar biology
authorswithroles_txt_mv	Coleine, Claudia @@aut@@ Stajich, Jason E. @@aut@@ Pombubpa, Nuttapon @@aut@@ Zucconi, Laura @@aut@@ Onofri, Silvano @@aut@@ Selbmann, Laura @@aut@@
publishDateDaySort_date	2020-02-17T00:00:00Z
hierarchy_top_id	271175427
dewey-sort	3570
id	SPR039040380
language_de	englisch
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author	Coleine, Claudia
spellingShingle	Coleine, Claudia ddc 570 bkl 42.00 misc Antarctica misc Cryptoendolithic communities misc Bacteria misc Fungi misc Metabarcoding misc Sampling effort Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities
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topic_title	570 ASE 42.00 bkl Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities Antarctica (dpeaa)DE-He213 Cryptoendolithic communities (dpeaa)DE-He213 Bacteria (dpeaa)DE-He213 Fungi (dpeaa)DE-He213 Metabarcoding (dpeaa)DE-He213 Sampling effort (dpeaa)DE-He213
topic	ddc 570 bkl 42.00 misc Antarctica misc Cryptoendolithic communities misc Bacteria misc Fungi misc Metabarcoding misc Sampling effort
topic_unstemmed	ddc 570 bkl 42.00 misc Antarctica misc Cryptoendolithic communities misc Bacteria misc Fungi misc Metabarcoding misc Sampling effort
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title	Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities
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title_full	Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities
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title_sort	sampling strategies to assess microbial diversity of antarctic cryptoendolithic communities
title_auth	Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities
abstract	Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities.
abstractGer	Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities.
abstract_unstemmed	Abstract Describing the total biodiversity of an environmental metacommunity is challenging due to the presence of cryptic and rare species and incompletely described taxonomy. How many samples to collect is a common issue that faces ecologists when designing fieldwork sampling. Nowadays, high-throughput sequencing allows examination of large numbers of samples enabling comprehensive biodiversity assessments. In this study, we sought to estimate how the scale of sampling affects accuracy of community diversity description in the McMurdo Dry Valleys (Southern Victoria Land) in Antarctica accounted as the closest Martian analogue on Earth, exhibiting extreme conditions such as low temperatures, wide thermal fluctuations, low nutrient availability and high UV radiation. We found that sampling effort, based on accumulation curves and statistical analysis, had a considerable impact on assessing species richness and composition in these ecosystems, confirming that a sampling as large as nine rock specimens was necessary to detect almost all fungal species present, but was not sufficient to capture whole bacterial assemblage. The sampling would require approximately four times more effort (~ 40 samples) for a comprehensive description of bacterial diversity. Our findings will be helpful to develop strategies to exhaustively describe the microbial diversity of Antarctic cryptoendolithic communities.
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title_short	Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities
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Sampling strategies to assess microbial diversity of Antarctic cryptoendolithic communities

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