Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations
Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Alam, Prawez [verfasserIn] Kamal, Y. T. [verfasserIn] Alqarni, Mohammed H. [verfasserIn] Zaatout, Hala H. [verfasserIn] Abdel-Kader, Maged S. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of planar chromatography, modern TLC - Budapest : Akadémiai Kiadó, 2001, 31(2018), 2 vom: 01. Apr., Seite 135-142 |
|---|---|
| Übergeordnetes Werk: |
volume:31 ; year:2018 ; number:2 ; day:01 ; month:04 ; pages:135-142 |
| Links: |
|---|
| DOI / URN: |
10.1556/1006.2018.31.2.7 |
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SPR039176975 |
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| 245 | 1 | 0 | |a Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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| 520 | |a Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. | ||
| 700 | 1 | |a Kamal, Y. T. |e verfasserin |4 aut | |
| 700 | 1 | |a Alqarni, Mohammed H. |e verfasserin |4 aut | |
| 700 | 1 | |a Zaatout, Hala H. |e verfasserin |4 aut | |
| 700 | 1 | |a Abdel-Kader, Maged S. |e verfasserin |4 aut | |
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10.1556/1006.2018.31.2.7 doi (DE-627)SPR039176975 (SPR)1006.2018.31.2.7-e DE-627 ger DE-627 rakwb eng 540 530 ASE Alam, Prawez verfasserin aut Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. Kamal, Y. T. verfasserin aut Alqarni, Mohammed H. verfasserin aut Zaatout, Hala H. verfasserin aut Abdel-Kader, Maged S. verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 31(2018), 2 vom: 01. Apr., Seite 135-142 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:31 year:2018 number:2 day:01 month:04 pages:135-142 https://dx.doi.org/10.1556/1006.2018.31.2.7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 31 2018 2 01 04 135-142 |
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10.1556/1006.2018.31.2.7 doi (DE-627)SPR039176975 (SPR)1006.2018.31.2.7-e DE-627 ger DE-627 rakwb eng 540 530 ASE Alam, Prawez verfasserin aut Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. Kamal, Y. T. verfasserin aut Alqarni, Mohammed H. verfasserin aut Zaatout, Hala H. verfasserin aut Abdel-Kader, Maged S. verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 31(2018), 2 vom: 01. Apr., Seite 135-142 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:31 year:2018 number:2 day:01 month:04 pages:135-142 https://dx.doi.org/10.1556/1006.2018.31.2.7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 31 2018 2 01 04 135-142 |
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10.1556/1006.2018.31.2.7 doi (DE-627)SPR039176975 (SPR)1006.2018.31.2.7-e DE-627 ger DE-627 rakwb eng 540 530 ASE Alam, Prawez verfasserin aut Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. Kamal, Y. T. verfasserin aut Alqarni, Mohammed H. verfasserin aut Zaatout, Hala H. verfasserin aut Abdel-Kader, Maged S. verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 31(2018), 2 vom: 01. Apr., Seite 135-142 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:31 year:2018 number:2 day:01 month:04 pages:135-142 https://dx.doi.org/10.1556/1006.2018.31.2.7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 31 2018 2 01 04 135-142 |
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10.1556/1006.2018.31.2.7 doi (DE-627)SPR039176975 (SPR)1006.2018.31.2.7-e DE-627 ger DE-627 rakwb eng 540 530 ASE Alam, Prawez verfasserin aut Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. Kamal, Y. T. verfasserin aut Alqarni, Mohammed H. verfasserin aut Zaatout, Hala H. verfasserin aut Abdel-Kader, Maged S. verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 31(2018), 2 vom: 01. Apr., Seite 135-142 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:31 year:2018 number:2 day:01 month:04 pages:135-142 https://dx.doi.org/10.1556/1006.2018.31.2.7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 31 2018 2 01 04 135-142 |
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10.1556/1006.2018.31.2.7 doi (DE-627)SPR039176975 (SPR)1006.2018.31.2.7-e DE-627 ger DE-627 rakwb eng 540 530 ASE Alam, Prawez verfasserin aut Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. Kamal, Y. T. verfasserin aut Alqarni, Mohammed H. verfasserin aut Zaatout, Hala H. verfasserin aut Abdel-Kader, Maged S. verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 31(2018), 2 vom: 01. Apr., Seite 135-142 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:31 year:2018 number:2 day:01 month:04 pages:135-142 https://dx.doi.org/10.1556/1006.2018.31.2.7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 31 2018 2 01 04 135-142 |
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| author |
Alam, Prawez |
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Alam, Prawez ddc 540 Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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540 530 ASE Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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Alam, Prawez Kamal, Y. T. Alqarni, Mohammed H. Zaatout, Hala H. Abdel-Kader, Maged S. |
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reversed-phase high-performance thin-layer chromatographic method for the simultaneous determination of glycyrrhizin and glabridin biomarkers in glycyrrhiza glabra roots, rhizomes and herbal formulations |
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Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
| abstract |
Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. |
| abstractGer |
Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. |
| abstract_unstemmed |
Summary A new rapid, simple, economical, and environment-friendly reversed- phase high-performance thin-layer chromatography (RPHPTLC) method has been established for the simultaneous determination of glycyrrhizin and glabridin in Glycyrrhiza glabra roots, rhizomes and selected herbal formulations. The method was carried out using RP-18 silica gel 60 $ F_{254S} $ HPTLC glass plates and methanol–water (7:3 v/v) as the mobile phase. The developed plates were scanned and quantified densitometrically at 256 and 233 nm for glycyrrhizin and glabridin, respectively. Glycyrrhizin and glabridin peaks from G. glabra roots and rhizomes and herbal formulations were identified by comparing their single spots at RF = 0.63 ± 0.02 and RF = 0.28 ± 0.01, respectively. Linear regression analysis revealed a good linear relationship between the peak areas and the amounts of glycyrrhizin and glabridin in the ranges of 1000–7000 and 100–700 ng $ band^{−1} $, respectively. The method was validated, in accordance with the International Conference on Harmonization (ICH) guidelines for precision, accuracy, and robustness. The proposed method will be useful to determine the therapeutic doses of glycyrrhizin and glabridin in herbal formulations as well as in bulk drug. |
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Reversed-Phase High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Glycyrrhizin and Glabridin Biomarkers in Glycyrrhiza glabra Roots, Rhizomes and Herbal Formulations |
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