The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides
Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation...
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Gespeichert in:
| Autor*in: |
Gwarda, Radosław Łukasz [verfasserIn] Szwerc, Wojciech [verfasserIn] Aletańska-Kozak, Monika [verfasserIn] Klimek-Turek, Anna [verfasserIn] Torbicz, Andrzej [verfasserIn] Chomicki, Adam [verfasserIn] Kocjan, Ryszard [verfasserIn] Matosiuk, Dariusz [verfasserIn] Dzido, Tadeusz Henryk [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of planar chromatography, modern TLC - Budapest : Akadémiai Kiadó, 2001, 30(2017), 5 vom: 01. Okt., Seite 375-385 |
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| Übergeordnetes Werk: |
volume:30 ; year:2017 ; number:5 ; day:01 ; month:10 ; pages:375-385 |
| Links: |
|---|
| DOI / URN: |
10.1556/1006.2017.30.5.6 |
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SPR039234169 |
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| 245 | 1 | 4 | |a The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides |
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| 520 | |a Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. | ||
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| 700 | 1 | |a Aletańska-Kozak, Monika |e verfasserin |4 aut | |
| 700 | 1 | |a Klimek-Turek, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Torbicz, Andrzej |e verfasserin |4 aut | |
| 700 | 1 | |a Chomicki, Adam |e verfasserin |4 aut | |
| 700 | 1 | |a Kocjan, Ryszard |e verfasserin |4 aut | |
| 700 | 1 | |a Matosiuk, Dariusz |e verfasserin |4 aut | |
| 700 | 1 | |a Dzido, Tadeusz Henryk |e verfasserin |4 aut | |
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10.1556/1006.2017.30.5.6 doi (DE-627)SPR039234169 (SPR)1006.2017.30.5.6-e DE-627 ger DE-627 rakwb eng 540 530 ASE Gwarda, Radosław Łukasz verfasserin aut The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. Szwerc, Wojciech verfasserin aut Aletańska-Kozak, Monika verfasserin aut Klimek-Turek, Anna verfasserin aut Torbicz, Andrzej verfasserin aut Chomicki, Adam verfasserin aut Kocjan, Ryszard verfasserin aut Matosiuk, Dariusz verfasserin aut Dzido, Tadeusz Henryk verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 30(2017), 5 vom: 01. Okt., Seite 375-385 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:30 year:2017 number:5 day:01 month:10 pages:375-385 https://dx.doi.org/10.1556/1006.2017.30.5.6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 30 2017 5 01 10 375-385 |
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10.1556/1006.2017.30.5.6 doi (DE-627)SPR039234169 (SPR)1006.2017.30.5.6-e DE-627 ger DE-627 rakwb eng 540 530 ASE Gwarda, Radosław Łukasz verfasserin aut The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. Szwerc, Wojciech verfasserin aut Aletańska-Kozak, Monika verfasserin aut Klimek-Turek, Anna verfasserin aut Torbicz, Andrzej verfasserin aut Chomicki, Adam verfasserin aut Kocjan, Ryszard verfasserin aut Matosiuk, Dariusz verfasserin aut Dzido, Tadeusz Henryk verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 30(2017), 5 vom: 01. Okt., Seite 375-385 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:30 year:2017 number:5 day:01 month:10 pages:375-385 https://dx.doi.org/10.1556/1006.2017.30.5.6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 30 2017 5 01 10 375-385 |
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10.1556/1006.2017.30.5.6 doi (DE-627)SPR039234169 (SPR)1006.2017.30.5.6-e DE-627 ger DE-627 rakwb eng 540 530 ASE Gwarda, Radosław Łukasz verfasserin aut The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. Szwerc, Wojciech verfasserin aut Aletańska-Kozak, Monika verfasserin aut Klimek-Turek, Anna verfasserin aut Torbicz, Andrzej verfasserin aut Chomicki, Adam verfasserin aut Kocjan, Ryszard verfasserin aut Matosiuk, Dariusz verfasserin aut Dzido, Tadeusz Henryk verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 30(2017), 5 vom: 01. Okt., Seite 375-385 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:30 year:2017 number:5 day:01 month:10 pages:375-385 https://dx.doi.org/10.1556/1006.2017.30.5.6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 30 2017 5 01 10 375-385 |
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10.1556/1006.2017.30.5.6 doi (DE-627)SPR039234169 (SPR)1006.2017.30.5.6-e DE-627 ger DE-627 rakwb eng 540 530 ASE Gwarda, Radosław Łukasz verfasserin aut The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. Szwerc, Wojciech verfasserin aut Aletańska-Kozak, Monika verfasserin aut Klimek-Turek, Anna verfasserin aut Torbicz, Andrzej verfasserin aut Chomicki, Adam verfasserin aut Kocjan, Ryszard verfasserin aut Matosiuk, Dariusz verfasserin aut Dzido, Tadeusz Henryk verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 30(2017), 5 vom: 01. Okt., Seite 375-385 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:30 year:2017 number:5 day:01 month:10 pages:375-385 https://dx.doi.org/10.1556/1006.2017.30.5.6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 30 2017 5 01 10 375-385 |
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10.1556/1006.2017.30.5.6 doi (DE-627)SPR039234169 (SPR)1006.2017.30.5.6-e DE-627 ger DE-627 rakwb eng 540 530 ASE Gwarda, Radosław Łukasz verfasserin aut The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. Szwerc, Wojciech verfasserin aut Aletańska-Kozak, Monika verfasserin aut Klimek-Turek, Anna verfasserin aut Torbicz, Andrzej verfasserin aut Chomicki, Adam verfasserin aut Kocjan, Ryszard verfasserin aut Matosiuk, Dariusz verfasserin aut Dzido, Tadeusz Henryk verfasserin aut Enthalten in Journal of planar chromatography, modern TLC Budapest : Akadémiai Kiadó, 2001 30(2017), 5 vom: 01. Okt., Seite 375-385 (DE-627)529764563 (DE-600)2304198-5 1789-0993 nnns volume:30 year:2017 number:5 day:01 month:10 pages:375-385 https://dx.doi.org/10.1556/1006.2017.30.5.6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_22 GBV_ILN_31 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2129 GBV_ILN_2190 GBV_ILN_4305 AR 30 2017 5 01 10 375-385 |
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The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides |
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Gwarda, Radosław Łukasz Szwerc, Wojciech Aletańska-Kozak, Monika Klimek-Turek, Anna Torbicz, Andrzej Chomicki, Adam Kocjan, Ryszard Matosiuk, Dariusz Dzido, Tadeusz Henryk |
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influence of metallic impurities on the free silanol activity of commercial thin-layer chromatography adsorbents demonstrated by retention changes of basic/amphoteric compounds such as peptides |
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The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides |
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Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. |
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Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. |
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Summary In our previous papers, we have mentioned some specific disruption of peptide zones shape and chromatogram distortion, when using mobile phase containing ion-pairing acids. This problem is investigated here. It concerns not only some specific separation conditions but also various separation systems with silica-based adsorbents and water—alcohol mobile phases. We show that the problem results from significant amount of metallic impurities present in the adsorbents investigated. Our results prove that these impurities strongly affect the activity of free silanol groups and thus the retention of basic or amphoteric compounds and the quality of the results obtained. The standard method of washing adsorbent layer with methanol is not effective against the impurities. Washing chromatographic plates with a solution containing an acid significantly reduces the amount of metallic impurities in the adsorbent, resulting in the reduction/elimination of these adverse effects. However, it also leads to the increase of heterogeneity of acidic groups activity and deterioration of separation system efficiency. Therefore, removing metal ions from the adsorbent may not always be advantageous. Avoiding of use of strong ion-pairing acids is also problematic and not always possible. Thus, the production of high-purity silica of homogenous activity seems to be the best and the most reliable solution of the problem described. |
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The Influence of Metallic Impurities on the Free Silanol Activity of Commercial Thin-Layer Chromatography Adsorbents Demonstrated by Retention Changes of Basic/Amphoteric Compounds Such As Peptides |
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