Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform
Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Berger, Jacob [verfasserIn] Valera, Enrique [verfasserIn] Jankelow, Aaron [verfasserIn] Garcia, Carlos [verfasserIn] Akhand, Manik [verfasserIn] Heredia, John [verfasserIn] Ghonge, Tanmay [verfasserIn] Liu, Cynthia [verfasserIn] Font-Bartumeus, Victor [verfasserIn] Oshana, Gina [verfasserIn] Tiao, Justin [verfasserIn] Bashir, Rashid [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biomedical microdevices - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998, 22(2020), 2 vom: 18. Mai |
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| Übergeordnetes Werk: |
volume:22 ; year:2020 ; number:2 ; day:18 ; month:05 |
| Links: |
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| DOI / URN: |
10.1007/s10544-020-00492-6 |
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| Katalog-ID: |
SPR039739910 |
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| 245 | 1 | 0 | |a Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
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| 520 | |a Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. | ||
| 650 | 4 | |a Microfluidic electrical impedance |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Protein detection |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Micro-bead assay |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a IL-6 and PCT |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Point-of-care diagnostics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Sepsis |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Valera, Enrique |e verfasserin |4 aut | |
| 700 | 1 | |a Jankelow, Aaron |e verfasserin |4 aut | |
| 700 | 1 | |a Garcia, Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Akhand, Manik |e verfasserin |4 aut | |
| 700 | 1 | |a Heredia, John |e verfasserin |4 aut | |
| 700 | 1 | |a Ghonge, Tanmay |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Cynthia |e verfasserin |4 aut | |
| 700 | 1 | |a Font-Bartumeus, Victor |e verfasserin |4 aut | |
| 700 | 1 | |a Oshana, Gina |e verfasserin |4 aut | |
| 700 | 1 | |a Tiao, Justin |e verfasserin |4 aut | |
| 700 | 1 | |a Bashir, Rashid |e verfasserin |4 aut | |
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10.1007/s10544-020-00492-6 doi (DE-627)SPR039739910 (SPR)s10544-020-00492-6-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Berger, Jacob verfasserin aut Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 Valera, Enrique verfasserin aut Jankelow, Aaron verfasserin aut Garcia, Carlos verfasserin aut Akhand, Manik verfasserin aut Heredia, John verfasserin aut Ghonge, Tanmay verfasserin aut Liu, Cynthia verfasserin aut Font-Bartumeus, Victor verfasserin aut Oshana, Gina verfasserin aut Tiao, Justin verfasserin aut Bashir, Rashid verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 22(2020), 2 vom: 18. Mai (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:22 year:2020 number:2 day:18 month:05 https://dx.doi.org/10.1007/s10544-020-00492-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 22 2020 2 18 05 |
| spelling |
10.1007/s10544-020-00492-6 doi (DE-627)SPR039739910 (SPR)s10544-020-00492-6-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Berger, Jacob verfasserin aut Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 Valera, Enrique verfasserin aut Jankelow, Aaron verfasserin aut Garcia, Carlos verfasserin aut Akhand, Manik verfasserin aut Heredia, John verfasserin aut Ghonge, Tanmay verfasserin aut Liu, Cynthia verfasserin aut Font-Bartumeus, Victor verfasserin aut Oshana, Gina verfasserin aut Tiao, Justin verfasserin aut Bashir, Rashid verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 22(2020), 2 vom: 18. Mai (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:22 year:2020 number:2 day:18 month:05 https://dx.doi.org/10.1007/s10544-020-00492-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 22 2020 2 18 05 |
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10.1007/s10544-020-00492-6 doi (DE-627)SPR039739910 (SPR)s10544-020-00492-6-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Berger, Jacob verfasserin aut Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 Valera, Enrique verfasserin aut Jankelow, Aaron verfasserin aut Garcia, Carlos verfasserin aut Akhand, Manik verfasserin aut Heredia, John verfasserin aut Ghonge, Tanmay verfasserin aut Liu, Cynthia verfasserin aut Font-Bartumeus, Victor verfasserin aut Oshana, Gina verfasserin aut Tiao, Justin verfasserin aut Bashir, Rashid verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 22(2020), 2 vom: 18. Mai (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:22 year:2020 number:2 day:18 month:05 https://dx.doi.org/10.1007/s10544-020-00492-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 22 2020 2 18 05 |
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10.1007/s10544-020-00492-6 doi (DE-627)SPR039739910 (SPR)s10544-020-00492-6-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Berger, Jacob verfasserin aut Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 Valera, Enrique verfasserin aut Jankelow, Aaron verfasserin aut Garcia, Carlos verfasserin aut Akhand, Manik verfasserin aut Heredia, John verfasserin aut Ghonge, Tanmay verfasserin aut Liu, Cynthia verfasserin aut Font-Bartumeus, Victor verfasserin aut Oshana, Gina verfasserin aut Tiao, Justin verfasserin aut Bashir, Rashid verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 22(2020), 2 vom: 18. Mai (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:22 year:2020 number:2 day:18 month:05 https://dx.doi.org/10.1007/s10544-020-00492-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 22 2020 2 18 05 |
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10.1007/s10544-020-00492-6 doi (DE-627)SPR039739910 (SPR)s10544-020-00492-6-e DE-627 ger DE-627 rakwb eng 570 ASE 44.00 bkl Berger, Jacob verfasserin aut Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 Valera, Enrique verfasserin aut Jankelow, Aaron verfasserin aut Garcia, Carlos verfasserin aut Akhand, Manik verfasserin aut Heredia, John verfasserin aut Ghonge, Tanmay verfasserin aut Liu, Cynthia verfasserin aut Font-Bartumeus, Victor verfasserin aut Oshana, Gina verfasserin aut Tiao, Justin verfasserin aut Bashir, Rashid verfasserin aut Enthalten in Biomedical microdevices Dordrecht [u.a.] : Springer Science + Business Media B.V, 1998 22(2020), 2 vom: 18. Mai (DE-627)320433269 (DE-600)2004019-2 1572-8781 nnns volume:22 year:2020 number:2 day:18 month:05 https://dx.doi.org/10.1007/s10544-020-00492-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 44.00 ASE AR 22 2020 2 18 05 |
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Microfluidic electrical impedance Protein detection Micro-bead assay IL-6 and PCT Point-of-care diagnostics Sepsis |
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Berger, Jacob @@aut@@ Valera, Enrique @@aut@@ Jankelow, Aaron @@aut@@ Garcia, Carlos @@aut@@ Akhand, Manik @@aut@@ Heredia, John @@aut@@ Ghonge, Tanmay @@aut@@ Liu, Cynthia @@aut@@ Font-Bartumeus, Victor @@aut@@ Oshana, Gina @@aut@@ Tiao, Justin @@aut@@ Bashir, Rashid @@aut@@ |
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|
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Berger, Jacob |
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Berger, Jacob ddc 570 bkl 44.00 misc Microfluidic electrical impedance misc Protein detection misc Micro-bead assay misc IL-6 and PCT misc Point-of-care diagnostics misc Sepsis Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
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570 ASE 44.00 bkl Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform Microfluidic electrical impedance (dpeaa)DE-He213 Protein detection (dpeaa)DE-He213 Micro-bead assay (dpeaa)DE-He213 IL-6 and PCT (dpeaa)DE-He213 Point-of-care diagnostics (dpeaa)DE-He213 Sepsis (dpeaa)DE-He213 |
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ddc 570 bkl 44.00 misc Microfluidic electrical impedance misc Protein detection misc Micro-bead assay misc IL-6 and PCT misc Point-of-care diagnostics misc Sepsis |
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ddc 570 bkl 44.00 misc Microfluidic electrical impedance misc Protein detection misc Micro-bead assay misc IL-6 and PCT misc Point-of-care diagnostics misc Sepsis |
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ddc 570 bkl 44.00 misc Microfluidic electrical impedance misc Protein detection misc Micro-bead assay misc IL-6 and PCT misc Point-of-care diagnostics misc Sepsis |
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Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
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Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
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Berger, Jacob |
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Biomedical microdevices |
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Berger, Jacob Valera, Enrique Jankelow, Aaron Garcia, Carlos Akhand, Manik Heredia, John Ghonge, Tanmay Liu, Cynthia Font-Bartumeus, Victor Oshana, Gina Tiao, Justin Bashir, Rashid |
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Berger, Jacob |
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10.1007/s10544-020-00492-6 |
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simultaneous electrical detection of il-6 and pct using a microfluidic biochip platform |
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Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
| abstract |
Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. |
| abstractGer |
Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. |
| abstract_unstemmed |
Abstract Sepsis, a life-threatening organ dysfunction caused by a dysregulated host response, leads the U.S in both mortality rate and cost of treatment. Sepsis treatment protocols currently rely on broad and non-specific parameters like heart and respiration rate, and temperature; however, studies show that biomarkers Interlukin-6 (IL-6) and Procalcitonin (PCT) correlate to sepsis progression and response to treatment. Prior work also suggests that using multi-parameter predictive analytics with biomarkers and clinical information can inform treatment to improve outcome. A point-of-care (POC) platform that provides information for multiple biomarkers can aid in the diagnosis and prognosis of potentially septic patients. Using impedance cytometry, microbead immunoassays, and biotin-streptavidin binding, we report a microfluidic POC system that correlates microbead capture to IL-6 and PCT concentrations. A multiplexed microbead immunoassay is developed and validated for simultaneous detection of both IL-6 and PCT from human plasma samples. Using the POC platform, we quantified plasma samples containing healthy, medium (~$ 10^{3} $pg/ml) and high (~$ 10^{5} $pg/ml) IL-6 and PCT concentrations with various levels of significance (P < 0.05-P < 0.00001) and validated the concept of this device as a POC platform for sepsis biomarkers. |
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Simultaneous electrical detection of IL-6 and PCT using a microfluidic biochip platform |
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Valera, Enrique Jankelow, Aaron Garcia, Carlos Akhand, Manik Heredia, John Ghonge, Tanmay Liu, Cynthia Font-Bartumeus, Victor Oshana, Gina Tiao, Justin Bashir, Rashid |
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| score |
7.4005537 |