Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen
Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed cultu...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wu, Xue-ling [verfasserIn] Wu, Xiao-yan [verfasserIn] Deng, Fan-fan [verfasserIn] Shen, Li [verfasserIn] Li, Jiao-kun [verfasserIn] Peng, Tang-jian [verfasserIn] Yu, Run-lan [verfasserIn] Liu, Yuan-dong [verfasserIn] Chen, Miao [verfasserIn] Qiu, Guan-zhou [verfasserIn] Zeng, Wei-min [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of Central South University of Technology - Changsha : CSUT, 1994, 27(2020), 5 vom: Mai, Seite 1386-1394 |
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| Übergeordnetes Werk: |
volume:27 ; year:2020 ; number:5 ; month:05 ; pages:1386-1394 |
| Links: |
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| DOI / URN: |
10.1007/s11771-020-4374-2 |
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| Katalog-ID: |
SPR040002578 |
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| 245 | 1 | 0 | |a Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
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| 520 | |a Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. | ||
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| 650 | 4 | |a chalcopyrite bioleaching |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a PEG-2000 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a cryopreservation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a microbial community |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Deng, Fan-fan |e verfasserin |4 aut | |
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| 700 | 1 | |a Li, Jiao-kun |e verfasserin |4 aut | |
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| 700 | 1 | |a Qiu, Guan-zhou |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Wei-min |e verfasserin |4 aut | |
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10.1007/s11771-020-4374-2 doi (DE-627)SPR040002578 (SPR)s11771-020-4374-2-e DE-627 ger DE-627 rakwb eng Wu, Xue-ling verfasserin aut Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 Wu, Xiao-yan verfasserin aut Deng, Fan-fan verfasserin aut Shen, Li verfasserin aut Li, Jiao-kun verfasserin aut Peng, Tang-jian verfasserin aut Yu, Run-lan verfasserin aut Liu, Yuan-dong verfasserin aut Chen, Miao verfasserin aut Qiu, Guan-zhou verfasserin aut Zeng, Wei-min verfasserin aut Enthalten in Journal of Central South University of Technology Changsha : CSUT, 1994 27(2020), 5 vom: Mai, Seite 1386-1394 (DE-627)531203670 (DE-600)2324899-3 1993-0666 nnns volume:27 year:2020 number:5 month:05 pages:1386-1394 https://dx.doi.org/10.1007/s11771-020-4374-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4753 AR 27 2020 5 05 1386-1394 |
| spelling |
10.1007/s11771-020-4374-2 doi (DE-627)SPR040002578 (SPR)s11771-020-4374-2-e DE-627 ger DE-627 rakwb eng Wu, Xue-ling verfasserin aut Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 Wu, Xiao-yan verfasserin aut Deng, Fan-fan verfasserin aut Shen, Li verfasserin aut Li, Jiao-kun verfasserin aut Peng, Tang-jian verfasserin aut Yu, Run-lan verfasserin aut Liu, Yuan-dong verfasserin aut Chen, Miao verfasserin aut Qiu, Guan-zhou verfasserin aut Zeng, Wei-min verfasserin aut Enthalten in Journal of Central South University of Technology Changsha : CSUT, 1994 27(2020), 5 vom: Mai, Seite 1386-1394 (DE-627)531203670 (DE-600)2324899-3 1993-0666 nnns volume:27 year:2020 number:5 month:05 pages:1386-1394 https://dx.doi.org/10.1007/s11771-020-4374-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4753 AR 27 2020 5 05 1386-1394 |
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10.1007/s11771-020-4374-2 doi (DE-627)SPR040002578 (SPR)s11771-020-4374-2-e DE-627 ger DE-627 rakwb eng Wu, Xue-ling verfasserin aut Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 Wu, Xiao-yan verfasserin aut Deng, Fan-fan verfasserin aut Shen, Li verfasserin aut Li, Jiao-kun verfasserin aut Peng, Tang-jian verfasserin aut Yu, Run-lan verfasserin aut Liu, Yuan-dong verfasserin aut Chen, Miao verfasserin aut Qiu, Guan-zhou verfasserin aut Zeng, Wei-min verfasserin aut Enthalten in Journal of Central South University of Technology Changsha : CSUT, 1994 27(2020), 5 vom: Mai, Seite 1386-1394 (DE-627)531203670 (DE-600)2324899-3 1993-0666 nnns volume:27 year:2020 number:5 month:05 pages:1386-1394 https://dx.doi.org/10.1007/s11771-020-4374-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4753 AR 27 2020 5 05 1386-1394 |
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10.1007/s11771-020-4374-2 doi (DE-627)SPR040002578 (SPR)s11771-020-4374-2-e DE-627 ger DE-627 rakwb eng Wu, Xue-ling verfasserin aut Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 Wu, Xiao-yan verfasserin aut Deng, Fan-fan verfasserin aut Shen, Li verfasserin aut Li, Jiao-kun verfasserin aut Peng, Tang-jian verfasserin aut Yu, Run-lan verfasserin aut Liu, Yuan-dong verfasserin aut Chen, Miao verfasserin aut Qiu, Guan-zhou verfasserin aut Zeng, Wei-min verfasserin aut Enthalten in Journal of Central South University of Technology Changsha : CSUT, 1994 27(2020), 5 vom: Mai, Seite 1386-1394 (DE-627)531203670 (DE-600)2324899-3 1993-0666 nnns volume:27 year:2020 number:5 month:05 pages:1386-1394 https://dx.doi.org/10.1007/s11771-020-4374-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4753 AR 27 2020 5 05 1386-1394 |
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10.1007/s11771-020-4374-2 doi (DE-627)SPR040002578 (SPR)s11771-020-4374-2-e DE-627 ger DE-627 rakwb eng Wu, Xue-ling verfasserin aut Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 Wu, Xiao-yan verfasserin aut Deng, Fan-fan verfasserin aut Shen, Li verfasserin aut Li, Jiao-kun verfasserin aut Peng, Tang-jian verfasserin aut Yu, Run-lan verfasserin aut Liu, Yuan-dong verfasserin aut Chen, Miao verfasserin aut Qiu, Guan-zhou verfasserin aut Zeng, Wei-min verfasserin aut Enthalten in Journal of Central South University of Technology Changsha : CSUT, 1994 27(2020), 5 vom: Mai, Seite 1386-1394 (DE-627)531203670 (DE-600)2324899-3 1993-0666 nnns volume:27 year:2020 number:5 month:05 pages:1386-1394 https://dx.doi.org/10.1007/s11771-020-4374-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2036 GBV_ILN_2037 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_2700 GBV_ILN_2817 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4346 GBV_ILN_4367 GBV_ILN_4392 GBV_ILN_4393 GBV_ILN_4753 AR 27 2020 5 05 1386-1394 |
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English |
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Enthalten in Journal of Central South University of Technology 27(2020), 5 vom: Mai, Seite 1386-1394 volume:27 year:2020 number:5 month:05 pages:1386-1394 |
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Enthalten in Journal of Central South University of Technology 27(2020), 5 vom: Mai, Seite 1386-1394 volume:27 year:2020 number:5 month:05 pages:1386-1394 |
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mixed cultures chalcopyrite bioleaching PEG-2000 cryopreservation microbial community |
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Wu, Xue-ling @@aut@@ Wu, Xiao-yan @@aut@@ Deng, Fan-fan @@aut@@ Shen, Li @@aut@@ Li, Jiao-kun @@aut@@ Peng, Tang-jian @@aut@@ Yu, Run-lan @@aut@@ Liu, Yuan-dong @@aut@@ Chen, Miao @@aut@@ Qiu, Guan-zhou @@aut@@ Zeng, Wei-min @@aut@@ |
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Wu, Xue-ling |
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Wu, Xue-ling misc mixed cultures misc chalcopyrite bioleaching misc PEG-2000 misc cryopreservation misc microbial community Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
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Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen mixed cultures (dpeaa)DE-He213 chalcopyrite bioleaching (dpeaa)DE-He213 PEG-2000 (dpeaa)DE-He213 cryopreservation (dpeaa)DE-He213 microbial community (dpeaa)DE-He213 |
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misc mixed cultures misc chalcopyrite bioleaching misc PEG-2000 misc cryopreservation misc microbial community |
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Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
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Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
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Wu, Xue-ling |
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Wu, Xue-ling Wu, Xiao-yan Deng, Fan-fan Shen, Li Li, Jiao-kun Peng, Tang-jian Yu, Run-lan Liu, Yuan-dong Chen, Miao Qiu, Guan-zhou Zeng, Wei-min |
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comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with peg-2000 in liquid nitrogen |
| title_auth |
Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
| abstract |
Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. |
| abstractGer |
Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. |
| abstract_unstemmed |
Abstract A mixed culture of bioleaching microorganisms was enriched. Then the mixed culture was preserved by liquid nitrogen cryopreservation for 6 months and 12 months, respectively, using PEG-2000 as the protective agent. The chalcopyrite leaching ability, activity and diversity of the mixed culture before and after preservation were compared. The results showed that the copper extraction rate was 95.7% in chalcopyrite bioleaching within 20 d by the original culture. After cryopreservation for 6 months and 12 months, the copper extraction rate of the mixed culture was 94.9% within 25 d and 93.6% within 35 d, respectively. The cell viability achieved 87% and 41% after being preserved for 6 months and 12 months, respectively. Furthermore, the ecology analysis identified Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus thermotolerans and Pseudomonas aeruginosa in the original mixed culture. After cryopreservation for 12 months, the composition of community changed, but the predominant microorganisms still existed. |
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Comparison of bioleaching of chalcopyrite concentrates with mixed culture after cryopreservation with PEG-2000 in liquid nitrogen |
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https://dx.doi.org/10.1007/s11771-020-4374-2 |
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Wu, Xiao-yan Deng, Fan-fan Shen, Li Li, Jiao-kun Peng, Tang-jian Yu, Run-lan Liu, Yuan-dong Chen, Miao Qiu, Guan-zhou Zeng, Wei-min |
| author2Str |
Wu, Xiao-yan Deng, Fan-fan Shen, Li Li, Jiao-kun Peng, Tang-jian Yu, Run-lan Liu, Yuan-dong Chen, Miao Qiu, Guan-zhou Zeng, Wei-min |
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10.1007/s11771-020-4374-2 |
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2024-07-04T02:28:12.701Z |
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|
| score |
7.399932 |