Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells
Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chen, Lingjun [verfasserIn] Yu, Xiaonan [verfasserIn] Ding, Haoxuan [verfasserIn] Zhao, Yang [verfasserIn] Hu, Caihong [verfasserIn] Feng, Jie [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2019 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biological trace element research - [S.l.] : Springer US, 1979, 196(2019), 2 vom: 15. Nov., Seite 481-493 |
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| Übergeordnetes Werk: |
volume:196 ; year:2019 ; number:2 ; day:15 ; month:11 ; pages:481-493 |
| Links: |
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| DOI / URN: |
10.1007/s12011-019-01948-4 |
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| Katalog-ID: |
SPR040100499 |
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| 245 | 1 | 0 | |a Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells |
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| 520 | |a Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. | ||
| 650 | 4 | |a Zinc salts |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Biosafety |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Oxidative stress |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Energy depletion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a IPEC-J2 cells |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Yu, Xiaonan |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Haoxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Caihong |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Jie |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biological trace element research |d [S.l.] : Springer US, 1979 |g 196(2019), 2 vom: 15. Nov., Seite 481-493 |w (DE-627)342893726 |w (DE-600)2072581-4 |x 1559-0720 |7 nnns |
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10.1007/s12011-019-01948-4 doi (DE-627)SPR040100499 (SPR)s12011-019-01948-4-e DE-627 ger DE-627 rakwb eng 570 ASE Chen, Lingjun verfasserin aut Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 Yu, Xiaonan verfasserin aut Ding, Haoxuan verfasserin aut Zhao, Yang verfasserin aut Hu, Caihong verfasserin aut Feng, Jie verfasserin aut Enthalten in Biological trace element research [S.l.] : Springer US, 1979 196(2019), 2 vom: 15. Nov., Seite 481-493 (DE-627)342893726 (DE-600)2072581-4 1559-0720 nnns volume:196 year:2019 number:2 day:15 month:11 pages:481-493 https://dx.doi.org/10.1007/s12011-019-01948-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 196 2019 2 15 11 481-493 |
| spelling |
10.1007/s12011-019-01948-4 doi (DE-627)SPR040100499 (SPR)s12011-019-01948-4-e DE-627 ger DE-627 rakwb eng 570 ASE Chen, Lingjun verfasserin aut Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 Yu, Xiaonan verfasserin aut Ding, Haoxuan verfasserin aut Zhao, Yang verfasserin aut Hu, Caihong verfasserin aut Feng, Jie verfasserin aut Enthalten in Biological trace element research [S.l.] : Springer US, 1979 196(2019), 2 vom: 15. Nov., Seite 481-493 (DE-627)342893726 (DE-600)2072581-4 1559-0720 nnns volume:196 year:2019 number:2 day:15 month:11 pages:481-493 https://dx.doi.org/10.1007/s12011-019-01948-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 196 2019 2 15 11 481-493 |
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10.1007/s12011-019-01948-4 doi (DE-627)SPR040100499 (SPR)s12011-019-01948-4-e DE-627 ger DE-627 rakwb eng 570 ASE Chen, Lingjun verfasserin aut Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 Yu, Xiaonan verfasserin aut Ding, Haoxuan verfasserin aut Zhao, Yang verfasserin aut Hu, Caihong verfasserin aut Feng, Jie verfasserin aut Enthalten in Biological trace element research [S.l.] : Springer US, 1979 196(2019), 2 vom: 15. Nov., Seite 481-493 (DE-627)342893726 (DE-600)2072581-4 1559-0720 nnns volume:196 year:2019 number:2 day:15 month:11 pages:481-493 https://dx.doi.org/10.1007/s12011-019-01948-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 196 2019 2 15 11 481-493 |
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10.1007/s12011-019-01948-4 doi (DE-627)SPR040100499 (SPR)s12011-019-01948-4-e DE-627 ger DE-627 rakwb eng 570 ASE Chen, Lingjun verfasserin aut Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 Yu, Xiaonan verfasserin aut Ding, Haoxuan verfasserin aut Zhao, Yang verfasserin aut Hu, Caihong verfasserin aut Feng, Jie verfasserin aut Enthalten in Biological trace element research [S.l.] : Springer US, 1979 196(2019), 2 vom: 15. Nov., Seite 481-493 (DE-627)342893726 (DE-600)2072581-4 1559-0720 nnns volume:196 year:2019 number:2 day:15 month:11 pages:481-493 https://dx.doi.org/10.1007/s12011-019-01948-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 196 2019 2 15 11 481-493 |
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10.1007/s12011-019-01948-4 doi (DE-627)SPR040100499 (SPR)s12011-019-01948-4-e DE-627 ger DE-627 rakwb eng 570 ASE Chen, Lingjun verfasserin aut Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 Yu, Xiaonan verfasserin aut Ding, Haoxuan verfasserin aut Zhao, Yang verfasserin aut Hu, Caihong verfasserin aut Feng, Jie verfasserin aut Enthalten in Biological trace element research [S.l.] : Springer US, 1979 196(2019), 2 vom: 15. Nov., Seite 481-493 (DE-627)342893726 (DE-600)2072581-4 1559-0720 nnns volume:196 year:2019 number:2 day:15 month:11 pages:481-493 https://dx.doi.org/10.1007/s12011-019-01948-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2070 GBV_ILN_2086 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2116 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 196 2019 2 15 11 481-493 |
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Enthalten in Biological trace element research 196(2019), 2 vom: 15. Nov., Seite 481-493 volume:196 year:2019 number:2 day:15 month:11 pages:481-493 |
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Enthalten in Biological trace element research 196(2019), 2 vom: 15. Nov., Seite 481-493 volume:196 year:2019 number:2 day:15 month:11 pages:481-493 |
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Zinc salts Biosafety Oxidative stress Energy depletion IPEC-J2 cells |
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Chen, Lingjun @@aut@@ Yu, Xiaonan @@aut@@ Ding, Haoxuan @@aut@@ Zhao, Yang @@aut@@ Hu, Caihong @@aut@@ Feng, Jie @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR040100499</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519184128.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2019 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12011-019-01948-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR040100499</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12011-019-01948-4-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chen, Lingjun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. 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Chen, Lingjun |
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Chen, Lingjun ddc 570 misc Zinc salts misc Biosafety misc Oxidative stress misc Energy depletion misc IPEC-J2 cells Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells |
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570 ASE Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells Zinc salts (dpeaa)DE-He213 Biosafety (dpeaa)DE-He213 Oxidative stress (dpeaa)DE-He213 Energy depletion (dpeaa)DE-He213 IPEC-J2 cells (dpeaa)DE-He213 |
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Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells |
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Chen, Lingjun Yu, Xiaonan Ding, Haoxuan Zhao, Yang Hu, Caihong Feng, Jie |
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comparing the influence of high doses of different zinc salts on oxidative stress and energy depletion in ipec-j2 cells |
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Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells |
| abstract |
Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. |
| abstractGer |
Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. |
| abstract_unstemmed |
Abstract The current study aimed to investigate the influence of four supplemental zinc salts (chelated: Zn glycine; non-chelated: Zn sulfate, Zn citrate, Zn gluconate) among different zinc concentrations (30–300 μM) on cell proliferation, oxidative stress, and energy depletion in intestinal porcine jejunum epithelial cells (IPEC-J2). Different zinc salts affected cell viability in a time- and dose-dependent manner, which was mainly dependent on the uptake of intracellular $ Zn^{2+} $. Intracellular $ Zn^{2+} $ of Zn sulfate has taken up almost twice as high as Zn glycine when cells were loaded with 100–200 μM zinc. After loading cells with 300 μM zinc, Zn glycine and Zn sulfate had a similar trend in accumulation of $ Zn^{2+} $. When the intracellular $ Zn^{2+} $ overloads, cells will gradually be damaged and subsequently die bearing biochemical features of necrosis or late apoptosis. Meanwhile, obviously, increased levels of intracellular ROS, mitochondrial ROS, MDA, and NO and decreased levels of GSH were observed. Excessive intracellular $ Zn^{2+} $ significantly decreased mitochondria membrane potential accompanied by an obvious loss of ATP and $ NAD^{+} $ levels. Overall, exposure to high doses of zinc salts caused cell damage, which was mainly dependent on the uptake of $ Zn^{2+} $. Zinc overload induced oxidative stress and energy depletion in IPEC-J2 cells, and the cell damage with non-chelated zinc addition was more serious than Zn glycine. |
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| container_issue |
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| title_short |
Comparing the Influence of High Doses of Different Zinc Salts on Oxidative Stress and Energy Depletion in IPEC-J2 Cells |
| url |
https://dx.doi.org/10.1007/s12011-019-01948-4 |
| remote_bool |
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| author2 |
Yu, Xiaonan Ding, Haoxuan Zhao, Yang Hu, Caihong Feng, Jie |
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| doi_str |
10.1007/s12011-019-01948-4 |
| up_date |
2024-07-03T13:47:39.134Z |
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| score |
7.4013023 |