Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses

Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Re...
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Autor*in:	Magalhàes, Lizandra G. [verfasserIn] Lima, Thais C. [verfasserIn] de Paula, Renato G. [verfasserIn] Moráis, Enyara R. [verfasserIn] Aguiar, Daniela P. [verfasserIn] Gardinassi, Luiz G. [verfasserIn] Garcia, Gustavo R. [verfasserIn] Laurentiz, Rosangela S. [verfasserIn] Rodrigues, Vanderlei [verfasserIn] Bastos, Jairo K. [verfasserIn] Filho, Ademar A. S. [verfasserIn] Yatsuda, Ana P. [verfasserIn] Cunha, Wilson R. [verfasserIn] Silva, Márcio L. A. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	(−)-6;6′-dinitrohinokinin Lignan Mass spectrometry Proteome Two-dimensional gel electrophoresis

Übergeordnetes Werk:	Enthalten in: Revista Brasileira de farmacognosia - Critiba : Soc. Brasileira de Farmacognosia, 1986, 26(2016), 3 vom: 03. März, Seite 334-341
Übergeordnetes Werk:	volume:26 ; year:2016 ; number:3 ; day:03 ; month:03 ; pages:334-341

Links:	Volltext

DOI / URN:	10.1016/j.bjp.2016.02.001

Katalog-ID:	SPR040175634

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520			\|a Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.
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allfields	10.1016/j.bjp.2016.02.001 doi (DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e DE-627 ger DE-627 rakwb eng 610 ASE Magalhàes, Lizandra G. verfasserin aut Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213 Lima, Thais C. verfasserin aut de Paula, Renato G. verfasserin aut Moráis, Enyara R. verfasserin aut Aguiar, Daniela P. verfasserin aut Gardinassi, Luiz G. verfasserin aut Garcia, Gustavo R. verfasserin aut Laurentiz, Rosangela S. verfasserin aut Rodrigues, Vanderlei verfasserin aut Bastos, Jairo K. verfasserin aut Filho, Ademar A. S. verfasserin aut Yatsuda, Ana P. verfasserin aut Cunha, Wilson R. verfasserin aut Silva, Márcio L. A. verfasserin aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 26(2016), 3 vom: 03. März, Seite 334-341 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:26 year:2016 number:3 day:03 month:03 pages:334-341 https://dx.doi.org/10.1016/j.bjp.2016.02.001 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2016 3 03 03 334-341
spelling	10.1016/j.bjp.2016.02.001 doi (DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e DE-627 ger DE-627 rakwb eng 610 ASE Magalhàes, Lizandra G. verfasserin aut Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213 Lima, Thais C. verfasserin aut de Paula, Renato G. verfasserin aut Moráis, Enyara R. verfasserin aut Aguiar, Daniela P. verfasserin aut Gardinassi, Luiz G. verfasserin aut Garcia, Gustavo R. verfasserin aut Laurentiz, Rosangela S. verfasserin aut Rodrigues, Vanderlei verfasserin aut Bastos, Jairo K. verfasserin aut Filho, Ademar A. S. verfasserin aut Yatsuda, Ana P. verfasserin aut Cunha, Wilson R. verfasserin aut Silva, Márcio L. A. verfasserin aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 26(2016), 3 vom: 03. März, Seite 334-341 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:26 year:2016 number:3 day:03 month:03 pages:334-341 https://dx.doi.org/10.1016/j.bjp.2016.02.001 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2016 3 03 03 334-341
allfields_unstemmed	10.1016/j.bjp.2016.02.001 doi (DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e DE-627 ger DE-627 rakwb eng 610 ASE Magalhàes, Lizandra G. verfasserin aut Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213 Lima, Thais C. verfasserin aut de Paula, Renato G. verfasserin aut Moráis, Enyara R. verfasserin aut Aguiar, Daniela P. verfasserin aut Gardinassi, Luiz G. verfasserin aut Garcia, Gustavo R. verfasserin aut Laurentiz, Rosangela S. verfasserin aut Rodrigues, Vanderlei verfasserin aut Bastos, Jairo K. verfasserin aut Filho, Ademar A. S. verfasserin aut Yatsuda, Ana P. verfasserin aut Cunha, Wilson R. verfasserin aut Silva, Márcio L. A. verfasserin aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 26(2016), 3 vom: 03. März, Seite 334-341 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:26 year:2016 number:3 day:03 month:03 pages:334-341 https://dx.doi.org/10.1016/j.bjp.2016.02.001 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2016 3 03 03 334-341
allfieldsGer	10.1016/j.bjp.2016.02.001 doi (DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e DE-627 ger DE-627 rakwb eng 610 ASE Magalhàes, Lizandra G. verfasserin aut Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213 Lima, Thais C. verfasserin aut de Paula, Renato G. verfasserin aut Moráis, Enyara R. verfasserin aut Aguiar, Daniela P. verfasserin aut Gardinassi, Luiz G. verfasserin aut Garcia, Gustavo R. verfasserin aut Laurentiz, Rosangela S. verfasserin aut Rodrigues, Vanderlei verfasserin aut Bastos, Jairo K. verfasserin aut Filho, Ademar A. S. verfasserin aut Yatsuda, Ana P. verfasserin aut Cunha, Wilson R. verfasserin aut Silva, Márcio L. A. verfasserin aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 26(2016), 3 vom: 03. März, Seite 334-341 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:26 year:2016 number:3 day:03 month:03 pages:334-341 https://dx.doi.org/10.1016/j.bjp.2016.02.001 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2016 3 03 03 334-341
allfieldsSound	10.1016/j.bjp.2016.02.001 doi (DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e DE-627 ger DE-627 rakwb eng 610 ASE Magalhàes, Lizandra G. verfasserin aut Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies. (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213 Lima, Thais C. verfasserin aut de Paula, Renato G. verfasserin aut Moráis, Enyara R. verfasserin aut Aguiar, Daniela P. verfasserin aut Gardinassi, Luiz G. verfasserin aut Garcia, Gustavo R. verfasserin aut Laurentiz, Rosangela S. verfasserin aut Rodrigues, Vanderlei verfasserin aut Bastos, Jairo K. verfasserin aut Filho, Ademar A. S. verfasserin aut Yatsuda, Ana P. verfasserin aut Cunha, Wilson R. verfasserin aut Silva, Márcio L. A. verfasserin aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 26(2016), 3 vom: 03. März, Seite 334-341 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:26 year:2016 number:3 day:03 month:03 pages:334-341 https://dx.doi.org/10.1016/j.bjp.2016.02.001 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 26 2016 3 03 03 334-341
language	English
source	Enthalten in Revista Brasileira de farmacognosia 26(2016), 3 vom: 03. März, Seite 334-341 volume:26 year:2016 number:3 day:03 month:03 pages:334-341
sourceStr	Enthalten in Revista Brasileira de farmacognosia 26(2016), 3 vom: 03. März, Seite 334-341 volume:26 year:2016 number:3 day:03 month:03 pages:334-341
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	(−)-6;6′-dinitrohinokinin Lignan Mass spectrometry Proteome Two-dimensional gel electrophoresis
dewey-raw	610
isfreeaccess_bool	true
container_title	Revista Brasileira de farmacognosia
authorswithroles_txt_mv	Magalhàes, Lizandra G. @@aut@@ Lima, Thais C. @@aut@@ de Paula, Renato G. @@aut@@ Moráis, Enyara R. @@aut@@ Aguiar, Daniela P. @@aut@@ Gardinassi, Luiz G. @@aut@@ Garcia, Gustavo R. @@aut@@ Laurentiz, Rosangela S. @@aut@@ Rodrigues, Vanderlei @@aut@@ Bastos, Jairo K. @@aut@@ Filho, Ademar A. S. @@aut@@ Yatsuda, Ana P. @@aut@@ Cunha, Wilson R. @@aut@@ Silva, Márcio L. A. @@aut@@
publishDateDaySort_date	2016-03-03T00:00:00Z
hierarchy_top_id	730275531
dewey-sort	3610
id	SPR040175634
language_de	englisch
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author	Magalhàes, Lizandra G.
spellingShingle	Magalhàes, Lizandra G. ddc 610 misc (−)-6;6′-dinitrohinokinin misc Lignan misc Mass spectrometry misc Proteome misc Two-dimensional gel electrophoresis Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
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topic_title	610 ASE Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses (−)-6;6′-dinitrohinokinin (dpeaa)DE-He213 Lignan (dpeaa)DE-He213 Mass spectrometry (dpeaa)DE-He213 Proteome (dpeaa)DE-He213 Two-dimensional gel electrophoresis (dpeaa)DE-He213
topic	ddc 610 misc (−)-6;6′-dinitrohinokinin misc Lignan misc Mass spectrometry misc Proteome misc Two-dimensional gel electrophoresis
topic_unstemmed	ddc 610 misc (−)-6;6′-dinitrohinokinin misc Lignan misc Mass spectrometry misc Proteome misc Two-dimensional gel electrophoresis
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title	Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
ctrlnum	(DE-627)SPR040175634 (SPR)j.bjp.2016.02.001-e
title_full	Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
author_sort	Magalhàes, Lizandra G.
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author_browse	Magalhàes, Lizandra G. Lima, Thais C. de Paula, Renato G. Moráis, Enyara R. Aguiar, Daniela P. Gardinassi, Luiz G. Garcia, Gustavo R. Laurentiz, Rosangela S. Rodrigues, Vanderlei Bastos, Jairo K. Filho, Ademar A. S. Yatsuda, Ana P. Cunha, Wilson R. Silva, Márcio L. A.
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author-letter	Magalhàes, Lizandra G.
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title_sort	effects of (−)-6,6′-dinitrohinokinin on adult worms of schistosoma mansoni: a proteomic analyses
title_auth	Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
abstract	Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.
abstractGer	Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.
abstract_unstemmed	Abstract Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematode flukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monotherapy with praziquantel reinforces the need for search and development of new therapeutic drugs. Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosoma mansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansoni soluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry. Enrichment for biological processes and predictive analyses of protein-protein interactions suggest that dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes, especially carbohydrate metabolism. In summary, this study provides an interesting approach to understand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound from lignan and for the development of new therapy strategies.
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container_issue	3
title_short	Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses
url	https://dx.doi.org/10.1016/j.bjp.2016.02.001
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author2	Lima, Thais C. de Paula, Renato G. Moráis, Enyara R. Aguiar, Daniela P. Gardinassi, Luiz G. Garcia, Gustavo R. Laurentiz, Rosangela S. Rodrigues, Vanderlei Bastos, Jairo K. Filho, Ademar A. S. Yatsuda, Ana P. Cunha, Wilson R. Silva, Márcio L. A.
author2Str	Lima, Thais C. de Paula, Renato G. Moráis, Enyara R. Aguiar, Daniela P. Gardinassi, Luiz G. Garcia, Gustavo R. Laurentiz, Rosangela S. Rodrigues, Vanderlei Bastos, Jairo K. Filho, Ademar A. S. Yatsuda, Ana P. Cunha, Wilson R. Silva, Márcio L. A.
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up_date	2024-07-03T14:16:53.083Z
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Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: a proteomic analyses

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