FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss
Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Pourfahraji Fakhrabadi, Marjan [verfasserIn] Kalantar, Seyed Mahdi [verfasserIn] Montazeri, Fatemeh [verfasserIn] Dehghani Ashkezari, Mahmood [verfasserIn] Pourfahraji Fakhrabadi, Majid [verfasserIn] Sabbagh Nejad Yazd, Samira [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Übergeordnetes Werk: |
Enthalten in: Middle East Fertility Society journal - [S.l.] : Elsevier, 2004, 25(2020), 1 vom: 01. Juli |
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| Übergeordnetes Werk: |
volume:25 ; year:2020 ; number:1 ; day:01 ; month:07 |
| Links: |
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| DOI / URN: |
10.1186/s43043-020-00031-6 |
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SPR040211088 |
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| 520 | |a Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. | ||
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10.1186/s43043-020-00031-6 doi (DE-627)SPR040211088 (SPR)s43043-020-00031-6-e DE-627 ger DE-627 rakwb eng 610 ASE Pourfahraji Fakhrabadi, Marjan verfasserin aut FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. FISH (dpeaa)DE-He213 RPL (dpeaa)DE-He213 Sperm (dpeaa)DE-He213 Aneuploidy (dpeaa)DE-He213 Kalantar, Seyed Mahdi verfasserin aut Montazeri, Fatemeh verfasserin aut Dehghani Ashkezari, Mahmood verfasserin aut Pourfahraji Fakhrabadi, Majid verfasserin aut Sabbagh Nejad Yazd, Samira verfasserin aut Enthalten in Middle East Fertility Society journal [S.l.] : Elsevier, 2004 25(2020), 1 vom: 01. Juli (DE-627)494831642 (DE-600)2196955-3 2090-3251 nnns volume:25 year:2020 number:1 day:01 month:07 https://dx.doi.org/10.1186/s43043-020-00031-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_206 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2020 1 01 07 |
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10.1186/s43043-020-00031-6 doi (DE-627)SPR040211088 (SPR)s43043-020-00031-6-e DE-627 ger DE-627 rakwb eng 610 ASE Pourfahraji Fakhrabadi, Marjan verfasserin aut FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. FISH (dpeaa)DE-He213 RPL (dpeaa)DE-He213 Sperm (dpeaa)DE-He213 Aneuploidy (dpeaa)DE-He213 Kalantar, Seyed Mahdi verfasserin aut Montazeri, Fatemeh verfasserin aut Dehghani Ashkezari, Mahmood verfasserin aut Pourfahraji Fakhrabadi, Majid verfasserin aut Sabbagh Nejad Yazd, Samira verfasserin aut Enthalten in Middle East Fertility Society journal [S.l.] : Elsevier, 2004 25(2020), 1 vom: 01. Juli (DE-627)494831642 (DE-600)2196955-3 2090-3251 nnns volume:25 year:2020 number:1 day:01 month:07 https://dx.doi.org/10.1186/s43043-020-00031-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_206 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2020 1 01 07 |
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10.1186/s43043-020-00031-6 doi (DE-627)SPR040211088 (SPR)s43043-020-00031-6-e DE-627 ger DE-627 rakwb eng 610 ASE Pourfahraji Fakhrabadi, Marjan verfasserin aut FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. FISH (dpeaa)DE-He213 RPL (dpeaa)DE-He213 Sperm (dpeaa)DE-He213 Aneuploidy (dpeaa)DE-He213 Kalantar, Seyed Mahdi verfasserin aut Montazeri, Fatemeh verfasserin aut Dehghani Ashkezari, Mahmood verfasserin aut Pourfahraji Fakhrabadi, Majid verfasserin aut Sabbagh Nejad Yazd, Samira verfasserin aut Enthalten in Middle East Fertility Society journal [S.l.] : Elsevier, 2004 25(2020), 1 vom: 01. Juli (DE-627)494831642 (DE-600)2196955-3 2090-3251 nnns volume:25 year:2020 number:1 day:01 month:07 https://dx.doi.org/10.1186/s43043-020-00031-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_206 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2020 1 01 07 |
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10.1186/s43043-020-00031-6 doi (DE-627)SPR040211088 (SPR)s43043-020-00031-6-e DE-627 ger DE-627 rakwb eng 610 ASE Pourfahraji Fakhrabadi, Marjan verfasserin aut FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. FISH (dpeaa)DE-He213 RPL (dpeaa)DE-He213 Sperm (dpeaa)DE-He213 Aneuploidy (dpeaa)DE-He213 Kalantar, Seyed Mahdi verfasserin aut Montazeri, Fatemeh verfasserin aut Dehghani Ashkezari, Mahmood verfasserin aut Pourfahraji Fakhrabadi, Majid verfasserin aut Sabbagh Nejad Yazd, Samira verfasserin aut Enthalten in Middle East Fertility Society journal [S.l.] : Elsevier, 2004 25(2020), 1 vom: 01. Juli (DE-627)494831642 (DE-600)2196955-3 2090-3251 nnns volume:25 year:2020 number:1 day:01 month:07 https://dx.doi.org/10.1186/s43043-020-00031-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_206 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2020 1 01 07 |
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10.1186/s43043-020-00031-6 doi (DE-627)SPR040211088 (SPR)s43043-020-00031-6-e DE-627 ger DE-627 rakwb eng 610 ASE Pourfahraji Fakhrabadi, Marjan verfasserin aut FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. FISH (dpeaa)DE-He213 RPL (dpeaa)DE-He213 Sperm (dpeaa)DE-He213 Aneuploidy (dpeaa)DE-He213 Kalantar, Seyed Mahdi verfasserin aut Montazeri, Fatemeh verfasserin aut Dehghani Ashkezari, Mahmood verfasserin aut Pourfahraji Fakhrabadi, Majid verfasserin aut Sabbagh Nejad Yazd, Samira verfasserin aut Enthalten in Middle East Fertility Society journal [S.l.] : Elsevier, 2004 25(2020), 1 vom: 01. Juli (DE-627)494831642 (DE-600)2196955-3 2090-3251 nnns volume:25 year:2020 number:1 day:01 month:07 https://dx.doi.org/10.1186/s43043-020-00031-6 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_206 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4305 AR 25 2020 1 01 07 |
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Enthalten in Middle East Fertility Society journal 25(2020), 1 vom: 01. Juli volume:25 year:2020 number:1 day:01 month:07 |
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Pourfahraji Fakhrabadi, Marjan Kalantar, Seyed Mahdi Montazeri, Fatemeh Dehghani Ashkezari, Mahmood Pourfahraji Fakhrabadi, Majid Sabbagh Nejad Yazd, Samira |
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fish-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss |
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FISH-based sperm aneuploidy screening in male partner of women with a history of recurrent pregnancy loss |
| abstract |
Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. |
| abstractGer |
Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. |
| abstract_unstemmed |
Background Recurrent pregnancy loss is defined as having the experience of three or more repeated pregnancy losses. Despite numerous studies, RPL etiology remains unclear; in this respect, many etiological factors have been thoroughly investigated. However, the contribution of male factors in RPL at the sperm aneuploidy has rarely been examined. The aim of the current study is to assess the existence of autosome and sex chromosome aneuploidy in sperm of men with history of recurrent pregnancy loss. In this prospective study, 50 men with a history of recurrent pregnancy loss were included as case group and 15 men with normal sperm analysis, without abortion history and with at least one child were included as control group. Two- and three-color fluorescence in situ hybridization technique was used for screening aneuploidy in 13, 18, 21, X, and Y chromosomes. Results Overall, the comparison of sperm aneuploidies in sex and autosome chromosomes (13, 21, 18) between case and control groups showed that chromosomal abnormality was significantly increased in men with RPL than men without history of RPL. Among all examined cells, about 18.28% nullisomy, 6.48% disomy, and 0.06% trisomy were observed. As well, two cells with XXYY and one cell with XXXY karyotype were observed. Among the analyzed chromosomes, the prevalence of nullisomy of chromosome 13 was higher than other studied chromosomes. Conclusion The results suggest the implication of sperm chromosomal abnormalities in recurrent pregnancy loss. Clinical application of FISH-based screening test is recommended for investigating sperm aneuploidy in RPL cases to counsel couples for making informed decision. |
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Kalantar, Seyed Mahdi Montazeri, Fatemeh Dehghani Ashkezari, Mahmood Pourfahraji Fakhrabadi, Majid Sabbagh Nejad Yazd, Samira |
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