An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy

Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed t...
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Gespeichert in:

Autor*in:	Yang, Meng [verfasserIn] Liu, Xiaoyan [verfasserIn] Luo, Qingqiong [verfasserIn] Xu, Lili [verfasserIn] Chen, Fuxiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Lemon derived extracellular vesicles Isolation Electrophoresis Dialysis Gastric cancer

Übergeordnetes Werk:	Enthalten in: Journal of nanobiotechnology - London : Biomed Central, 2003, 18(2020), 1 vom: 20. Juli
Übergeordnetes Werk:	volume:18 ; year:2020 ; number:1 ; day:20 ; month:07

Links:	Volltext

DOI / URN:	10.1186/s12951-020-00656-9

Katalog-ID:	SPR040396231

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520			\|a Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.
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650		4	\|a Isolation \|7 (dpeaa)DE-He213
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650		4	\|a Gastric cancer \|7 (dpeaa)DE-He213
700	1		\|a Liu, Xiaoyan \|e verfasserin \|4 aut
700	1		\|a Luo, Qingqiong \|e verfasserin \|4 aut
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700	1		\|a Chen, Fuxiang \|e verfasserin \|4 aut
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author_variant	m y my x l xl q l ql l x lx f c fc
matchkey_str	article:14773155:2020----::nfiinmtotioaeeodrvdxrcluavscef
hierarchy_sort_str	2020
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publishDate	2020
allfields	10.1186/s12951-020-00656-9 doi (DE-627)SPR040396231 (SPR)s12951-020-00656-9-e DE-627 ger DE-627 rakwb eng 540 610 ASE 58.30 bkl 50.94 bkl Yang, Meng verfasserin aut An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Liu, Xiaoyan verfasserin aut Luo, Qingqiong verfasserin aut Xu, Lili verfasserin aut Chen, Fuxiang verfasserin aut Enthalten in Journal of nanobiotechnology London : Biomed Central, 2003 18(2020), 1 vom: 20. Juli (DE-627)362770328 (DE-600)2100022-0 1477-3155 nnns volume:18 year:2020 number:1 day:20 month:07 https://dx.doi.org/10.1186/s12951-020-00656-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE 50.94 ASE AR 18 2020 1 20 07
spelling	10.1186/s12951-020-00656-9 doi (DE-627)SPR040396231 (SPR)s12951-020-00656-9-e DE-627 ger DE-627 rakwb eng 540 610 ASE 58.30 bkl 50.94 bkl Yang, Meng verfasserin aut An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Liu, Xiaoyan verfasserin aut Luo, Qingqiong verfasserin aut Xu, Lili verfasserin aut Chen, Fuxiang verfasserin aut Enthalten in Journal of nanobiotechnology London : Biomed Central, 2003 18(2020), 1 vom: 20. Juli (DE-627)362770328 (DE-600)2100022-0 1477-3155 nnns volume:18 year:2020 number:1 day:20 month:07 https://dx.doi.org/10.1186/s12951-020-00656-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE 50.94 ASE AR 18 2020 1 20 07
allfields_unstemmed	10.1186/s12951-020-00656-9 doi (DE-627)SPR040396231 (SPR)s12951-020-00656-9-e DE-627 ger DE-627 rakwb eng 540 610 ASE 58.30 bkl 50.94 bkl Yang, Meng verfasserin aut An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Liu, Xiaoyan verfasserin aut Luo, Qingqiong verfasserin aut Xu, Lili verfasserin aut Chen, Fuxiang verfasserin aut Enthalten in Journal of nanobiotechnology London : Biomed Central, 2003 18(2020), 1 vom: 20. Juli (DE-627)362770328 (DE-600)2100022-0 1477-3155 nnns volume:18 year:2020 number:1 day:20 month:07 https://dx.doi.org/10.1186/s12951-020-00656-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE 50.94 ASE AR 18 2020 1 20 07
allfieldsGer	10.1186/s12951-020-00656-9 doi (DE-627)SPR040396231 (SPR)s12951-020-00656-9-e DE-627 ger DE-627 rakwb eng 540 610 ASE 58.30 bkl 50.94 bkl Yang, Meng verfasserin aut An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Liu, Xiaoyan verfasserin aut Luo, Qingqiong verfasserin aut Xu, Lili verfasserin aut Chen, Fuxiang verfasserin aut Enthalten in Journal of nanobiotechnology London : Biomed Central, 2003 18(2020), 1 vom: 20. Juli (DE-627)362770328 (DE-600)2100022-0 1477-3155 nnns volume:18 year:2020 number:1 day:20 month:07 https://dx.doi.org/10.1186/s12951-020-00656-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE 50.94 ASE AR 18 2020 1 20 07
allfieldsSound	10.1186/s12951-020-00656-9 doi (DE-627)SPR040396231 (SPR)s12951-020-00656-9-e DE-627 ger DE-627 rakwb eng 540 610 ASE 58.30 bkl 50.94 bkl Yang, Meng verfasserin aut An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213 Liu, Xiaoyan verfasserin aut Luo, Qingqiong verfasserin aut Xu, Lili verfasserin aut Chen, Fuxiang verfasserin aut Enthalten in Journal of nanobiotechnology London : Biomed Central, 2003 18(2020), 1 vom: 20. Juli (DE-627)362770328 (DE-600)2100022-0 1477-3155 nnns volume:18 year:2020 number:1 day:20 month:07 https://dx.doi.org/10.1186/s12951-020-00656-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 ASE 50.94 ASE AR 18 2020 1 20 07
language	English
source	Enthalten in Journal of nanobiotechnology 18(2020), 1 vom: 20. Juli volume:18 year:2020 number:1 day:20 month:07
sourceStr	Enthalten in Journal of nanobiotechnology 18(2020), 1 vom: 20. Juli volume:18 year:2020 number:1 day:20 month:07
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topic_facet	Lemon derived extracellular vesicles Isolation Electrophoresis Dialysis Gastric cancer
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authorswithroles_txt_mv	Yang, Meng @@aut@@ Liu, Xiaoyan @@aut@@ Luo, Qingqiong @@aut@@ Xu, Lili @@aut@@ Chen, Fuxiang @@aut@@
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Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lemon derived extracellular vesicles</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Isolation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Electrophoresis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Dialysis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gastric cancer</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Xiaoyan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Luo, Qingqiong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Lili</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Fuxiang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of nanobiotechnology</subfield><subfield code="d">London : Biomed Central, 2003</subfield><subfield code="g">18(2020), 1 vom: 20. 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author	Yang, Meng
spellingShingle	Yang, Meng ddc 540 bkl 58.30 bkl 50.94 misc Lemon derived extracellular vesicles misc Isolation misc Electrophoresis misc Dialysis misc Gastric cancer An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
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topic_title	540 610 ASE 58.30 bkl 50.94 bkl An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy Lemon derived extracellular vesicles (dpeaa)DE-He213 Isolation (dpeaa)DE-He213 Electrophoresis (dpeaa)DE-He213 Dialysis (dpeaa)DE-He213 Gastric cancer (dpeaa)DE-He213
topic	ddc 540 bkl 58.30 bkl 50.94 misc Lemon derived extracellular vesicles misc Isolation misc Electrophoresis misc Dialysis misc Gastric cancer
topic_unstemmed	ddc 540 bkl 58.30 bkl 50.94 misc Lemon derived extracellular vesicles misc Isolation misc Electrophoresis misc Dialysis misc Gastric cancer
topic_browse	ddc 540 bkl 58.30 bkl 50.94 misc Lemon derived extracellular vesicles misc Isolation misc Electrophoresis misc Dialysis misc Gastric cancer
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title	An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
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title_full	An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
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journal	Journal of nanobiotechnology
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author_browse	Yang, Meng Liu, Xiaoyan Luo, Qingqiong Xu, Lili Chen, Fuxiang
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author-letter	Yang, Meng
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dewey-full	540 610
author2-role	verfasserin
title_sort	efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
title_auth	An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
abstract	Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.
abstractGer	Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.
abstract_unstemmed	Background Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle.
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title_short	An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
url	https://dx.doi.org/10.1186/s12951-020-00656-9
remote_bool	true
author2	Liu, Xiaoyan Luo, Qingqiong Xu, Lili Chen, Fuxiang
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doi_str	10.1186/s12951-020-00656-9
up_date	2024-07-03T15:43:28.825Z
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Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. 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An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy

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