Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm
Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Varada, Burdekar [verfasserIn] Pradeep, Appukuttan Nair R. [verfasserIn] Awasthi, Arvind K. [verfasserIn] Ponnuvel, Kangayam M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: International journal of tropical insect science - [Cham] : Springer International Publishing, 2004, 40(2020), 3 vom: 14. Juli, Seite 483-491 |
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| Übergeordnetes Werk: |
volume:40 ; year:2020 ; number:3 ; day:14 ; month:07 ; pages:483-491 |
| Links: |
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| DOI / URN: |
10.1007/s42690-020-00201-z |
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| Katalog-ID: |
SPR040733009 |
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| 245 | 1 | 0 | |a Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
| 264 | 1 | |c 2020 | |
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| 520 | |a Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. | ||
| 650 | 4 | |a NPV tolerance |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Transgenic silkworm |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a RNA interference |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a NPV genes |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Inbreeding |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Pradeep, Appukuttan Nair R. |e verfasserin |4 aut | |
| 700 | 1 | |a Awasthi, Arvind K. |e verfasserin |4 aut | |
| 700 | 1 | |a Ponnuvel, Kangayam M. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of tropical insect science |d [Cham] : Springer International Publishing, 2004 |g 40(2020), 3 vom: 14. Juli, Seite 483-491 |w (DE-627)470546743 |w (DE-600)2165935-7 |x 1742-7592 |7 nnns |
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10.1007/s42690-020-00201-z doi (DE-627)SPR040733009 (SPR)s42690-020-00201-z-e DE-627 ger DE-627 rakwb eng 590 ASE 570 ASE Varada, Burdekar verfasserin aut Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 Pradeep, Appukuttan Nair R. verfasserin aut Awasthi, Arvind K. verfasserin aut Ponnuvel, Kangayam M. verfasserin aut Enthalten in International journal of tropical insect science [Cham] : Springer International Publishing, 2004 40(2020), 3 vom: 14. Juli, Seite 483-491 (DE-627)470546743 (DE-600)2165935-7 1742-7592 nnns volume:40 year:2020 number:3 day:14 month:07 pages:483-491 https://dx.doi.org/10.1007/s42690-020-00201-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2020 3 14 07 483-491 |
| spelling |
10.1007/s42690-020-00201-z doi (DE-627)SPR040733009 (SPR)s42690-020-00201-z-e DE-627 ger DE-627 rakwb eng 590 ASE 570 ASE Varada, Burdekar verfasserin aut Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 Pradeep, Appukuttan Nair R. verfasserin aut Awasthi, Arvind K. verfasserin aut Ponnuvel, Kangayam M. verfasserin aut Enthalten in International journal of tropical insect science [Cham] : Springer International Publishing, 2004 40(2020), 3 vom: 14. Juli, Seite 483-491 (DE-627)470546743 (DE-600)2165935-7 1742-7592 nnns volume:40 year:2020 number:3 day:14 month:07 pages:483-491 https://dx.doi.org/10.1007/s42690-020-00201-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2020 3 14 07 483-491 |
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10.1007/s42690-020-00201-z doi (DE-627)SPR040733009 (SPR)s42690-020-00201-z-e DE-627 ger DE-627 rakwb eng 590 ASE 570 ASE Varada, Burdekar verfasserin aut Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 Pradeep, Appukuttan Nair R. verfasserin aut Awasthi, Arvind K. verfasserin aut Ponnuvel, Kangayam M. verfasserin aut Enthalten in International journal of tropical insect science [Cham] : Springer International Publishing, 2004 40(2020), 3 vom: 14. Juli, Seite 483-491 (DE-627)470546743 (DE-600)2165935-7 1742-7592 nnns volume:40 year:2020 number:3 day:14 month:07 pages:483-491 https://dx.doi.org/10.1007/s42690-020-00201-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2020 3 14 07 483-491 |
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10.1007/s42690-020-00201-z doi (DE-627)SPR040733009 (SPR)s42690-020-00201-z-e DE-627 ger DE-627 rakwb eng 590 ASE 570 ASE Varada, Burdekar verfasserin aut Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 Pradeep, Appukuttan Nair R. verfasserin aut Awasthi, Arvind K. verfasserin aut Ponnuvel, Kangayam M. verfasserin aut Enthalten in International journal of tropical insect science [Cham] : Springer International Publishing, 2004 40(2020), 3 vom: 14. Juli, Seite 483-491 (DE-627)470546743 (DE-600)2165935-7 1742-7592 nnns volume:40 year:2020 number:3 day:14 month:07 pages:483-491 https://dx.doi.org/10.1007/s42690-020-00201-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2020 3 14 07 483-491 |
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10.1007/s42690-020-00201-z doi (DE-627)SPR040733009 (SPR)s42690-020-00201-z-e DE-627 ger DE-627 rakwb eng 590 ASE 570 ASE Varada, Burdekar verfasserin aut Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 Pradeep, Appukuttan Nair R. verfasserin aut Awasthi, Arvind K. verfasserin aut Ponnuvel, Kangayam M. verfasserin aut Enthalten in International journal of tropical insect science [Cham] : Springer International Publishing, 2004 40(2020), 3 vom: 14. Juli, Seite 483-491 (DE-627)470546743 (DE-600)2165935-7 1742-7592 nnns volume:40 year:2020 number:3 day:14 month:07 pages:483-491 https://dx.doi.org/10.1007/s42690-020-00201-z lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_266 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 40 2020 3 14 07 483-491 |
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Enthalten in International journal of tropical insect science 40(2020), 3 vom: 14. Juli, Seite 483-491 volume:40 year:2020 number:3 day:14 month:07 pages:483-491 |
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NPV tolerance Transgenic silkworm RNA interference NPV genes Inbreeding |
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International journal of tropical insect science |
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Varada, Burdekar @@aut@@ Pradeep, Appukuttan Nair R. @@aut@@ Awasthi, Arvind K. @@aut@@ Ponnuvel, Kangayam M. @@aut@@ |
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Varada, Burdekar |
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Varada, Burdekar ddc 590 ddc 570 misc NPV tolerance misc Transgenic silkworm misc RNA interference misc NPV genes misc Inbreeding Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
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590 ASE 570 ASE Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm NPV tolerance (dpeaa)DE-He213 Transgenic silkworm (dpeaa)DE-He213 RNA interference (dpeaa)DE-He213 NPV genes (dpeaa)DE-He213 Inbreeding (dpeaa)DE-He213 |
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ddc 590 ddc 570 misc NPV tolerance misc Transgenic silkworm misc RNA interference misc NPV genes misc Inbreeding |
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Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
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Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
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International journal of tropical insect science |
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Varada, Burdekar Pradeep, Appukuttan Nair R. Awasthi, Arvind K. Ponnuvel, Kangayam M. |
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modulation of npv gene expression pattern and retention of rnai- based antiviral activity in inbred transgenic silkworm |
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Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
| abstract |
Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. |
| abstractGer |
Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. |
| abstract_unstemmed |
Abstract Infections due to Bombyx mori nucleopolyhedro virus (BmNPV) in commercially important silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) causes up to 50% loss to silk production in India. Increased resistance to NPV through introduction of double stranded RNA transgenes against multiple NPV genes is reported in B. mori. However, stability of the RNAi - mediated antiviral activity has not been determined after several generations of inbred transgenic B. mori. Hence, we examined the NPV multiplication rate and induction of the disease symptoms in NPV- infected transgenic and non-transgenic silkworm after 35 generations. Significantly higher multiplication rate of NPV supported by higher copy number of gp-41 gene was observed in non- transgenic larvae as compared to transgenic larvae. Expression of NPV genes, ie-1, lef1 and p74 enhanced significantly in non-transgenic larva as compared to transgenic larva after NPV infection. Strong positive correlation was observed in the expression pattern of NPV genes in transgenic larvae which was absent in non-transgenic larvae. This indicated co-regulation of NPV gene expression in the presence of transgenes. |
| collection_details |
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| container_issue |
3 |
| title_short |
Modulation of NPV gene expression pattern and retention of RNAi- based antiviral activity in inbred transgenic silkworm |
| url |
https://dx.doi.org/10.1007/s42690-020-00201-z |
| remote_bool |
true |
| author2 |
Pradeep, Appukuttan Nair R. Awasthi, Arvind K. Ponnuvel, Kangayam M. |
| author2Str |
Pradeep, Appukuttan Nair R. Awasthi, Arvind K. Ponnuvel, Kangayam M. |
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| doi_str |
10.1007/s42690-020-00201-z |
| up_date |
2024-07-03T17:54:56.223Z |
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| score |
7.399748 |