Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction

Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang d...
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Gespeichert in:

Autor*in:	Wang, Jinglong [verfasserIn] Zheng, Dandan [verfasserIn] Xu, Nan [verfasserIn] Zhang, Chao [verfasserIn] Wang, Yingzi [verfasserIn] Sun, Xiumei [verfasserIn] Zhang, Zhaowang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Erhuang decoction Semi-bionic enzyme extraction HPLC-DAD-ESI-MS Bioactive constituents Identification

Übergeordnetes Werk:	Enthalten in: Journal of analytical science and technology - Taejŏn : SpringerOpen, 2010, 11(2020), 1 vom: 28. Aug.
Übergeordnetes Werk:	volume:11 ; year:2020 ; number:1 ; day:28 ; month:08

Links:	Volltext

DOI / URN:	10.1186/s40543-020-00236-4

Katalog-ID:	SPR04079654X

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520			\|a Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.
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allfields	10.1186/s40543-020-00236-4 doi (DE-627)SPR04079654X (SPR)s40543-020-00236-4-e DE-627 ger DE-627 rakwb eng 540 ASE 30.00 bkl 50.00 bkl Wang, Jinglong verfasserin aut Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction. Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Zheng, Dandan verfasserin aut Xu, Nan verfasserin aut Zhang, Chao verfasserin aut Wang, Yingzi verfasserin aut Sun, Xiumei verfasserin aut Zhang, Zhaowang verfasserin aut Enthalten in Journal of analytical science and technology Taejŏn : SpringerOpen, 2010 11(2020), 1 vom: 28. Aug. (DE-627)669832553 (DE-600)2632347-3 2093-3371 nnns volume:11 year:2020 number:1 day:28 month:08 https://dx.doi.org/10.1186/s40543-020-00236-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 30.00 ASE 50.00 ASE AR 11 2020 1 28 08
spelling	10.1186/s40543-020-00236-4 doi (DE-627)SPR04079654X (SPR)s40543-020-00236-4-e DE-627 ger DE-627 rakwb eng 540 ASE 30.00 bkl 50.00 bkl Wang, Jinglong verfasserin aut Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction. Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Zheng, Dandan verfasserin aut Xu, Nan verfasserin aut Zhang, Chao verfasserin aut Wang, Yingzi verfasserin aut Sun, Xiumei verfasserin aut Zhang, Zhaowang verfasserin aut Enthalten in Journal of analytical science and technology Taejŏn : SpringerOpen, 2010 11(2020), 1 vom: 28. Aug. (DE-627)669832553 (DE-600)2632347-3 2093-3371 nnns volume:11 year:2020 number:1 day:28 month:08 https://dx.doi.org/10.1186/s40543-020-00236-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 30.00 ASE 50.00 ASE AR 11 2020 1 28 08
allfields_unstemmed	10.1186/s40543-020-00236-4 doi (DE-627)SPR04079654X (SPR)s40543-020-00236-4-e DE-627 ger DE-627 rakwb eng 540 ASE 30.00 bkl 50.00 bkl Wang, Jinglong verfasserin aut Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction. Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Zheng, Dandan verfasserin aut Xu, Nan verfasserin aut Zhang, Chao verfasserin aut Wang, Yingzi verfasserin aut Sun, Xiumei verfasserin aut Zhang, Zhaowang verfasserin aut Enthalten in Journal of analytical science and technology Taejŏn : SpringerOpen, 2010 11(2020), 1 vom: 28. Aug. (DE-627)669832553 (DE-600)2632347-3 2093-3371 nnns volume:11 year:2020 number:1 day:28 month:08 https://dx.doi.org/10.1186/s40543-020-00236-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 30.00 ASE 50.00 ASE AR 11 2020 1 28 08
allfieldsGer	10.1186/s40543-020-00236-4 doi (DE-627)SPR04079654X (SPR)s40543-020-00236-4-e DE-627 ger DE-627 rakwb eng 540 ASE 30.00 bkl 50.00 bkl Wang, Jinglong verfasserin aut Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction. Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Zheng, Dandan verfasserin aut Xu, Nan verfasserin aut Zhang, Chao verfasserin aut Wang, Yingzi verfasserin aut Sun, Xiumei verfasserin aut Zhang, Zhaowang verfasserin aut Enthalten in Journal of analytical science and technology Taejŏn : SpringerOpen, 2010 11(2020), 1 vom: 28. Aug. (DE-627)669832553 (DE-600)2632347-3 2093-3371 nnns volume:11 year:2020 number:1 day:28 month:08 https://dx.doi.org/10.1186/s40543-020-00236-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 30.00 ASE 50.00 ASE AR 11 2020 1 28 08
allfieldsSound	10.1186/s40543-020-00236-4 doi (DE-627)SPR04079654X (SPR)s40543-020-00236-4-e DE-627 ger DE-627 rakwb eng 540 ASE 30.00 bkl 50.00 bkl Wang, Jinglong verfasserin aut Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction. Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213 Zheng, Dandan verfasserin aut Xu, Nan verfasserin aut Zhang, Chao verfasserin aut Wang, Yingzi verfasserin aut Sun, Xiumei verfasserin aut Zhang, Zhaowang verfasserin aut Enthalten in Journal of analytical science and technology Taejŏn : SpringerOpen, 2010 11(2020), 1 vom: 28. Aug. (DE-627)669832553 (DE-600)2632347-3 2093-3371 nnns volume:11 year:2020 number:1 day:28 month:08 https://dx.doi.org/10.1186/s40543-020-00236-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2027 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 30.00 ASE 50.00 ASE AR 11 2020 1 28 08
language	English
source	Enthalten in Journal of analytical science and technology 11(2020), 1 vom: 28. Aug. volume:11 year:2020 number:1 day:28 month:08
sourceStr	Enthalten in Journal of analytical science and technology 11(2020), 1 vom: 28. Aug. volume:11 year:2020 number:1 day:28 month:08
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topic_facet	Erhuang decoction Semi-bionic enzyme extraction HPLC-DAD-ESI-MS Bioactive constituents Identification
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authorswithroles_txt_mv	Wang, Jinglong @@aut@@ Zheng, Dandan @@aut@@ Xu, Nan @@aut@@ Zhang, Chao @@aut@@ Wang, Yingzi @@aut@@ Sun, Xiumei @@aut@@ Zhang, Zhaowang @@aut@@
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author	Wang, Jinglong
spellingShingle	Wang, Jinglong ddc 540 bkl 30.00 bkl 50.00 misc Erhuang decoction misc Semi-bionic enzyme extraction misc HPLC-DAD-ESI-MS misc Bioactive constituents misc Identification Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction
authorStr	Wang, Jinglong
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topic_title	540 ASE 30.00 bkl 50.00 bkl Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction Erhuang decoction (dpeaa)DE-He213 Semi-bionic enzyme extraction (dpeaa)DE-He213 HPLC-DAD-ESI-MS (dpeaa)DE-He213 Bioactive constituents (dpeaa)DE-He213 Identification (dpeaa)DE-He213
topic	ddc 540 bkl 30.00 bkl 50.00 misc Erhuang decoction misc Semi-bionic enzyme extraction misc HPLC-DAD-ESI-MS misc Bioactive constituents misc Identification
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title	Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction
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title_full	Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction
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author_browse	Wang, Jinglong Zheng, Dandan Xu, Nan Zhang, Chao Wang, Yingzi Sun, Xiumei Zhang, Zhaowang
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title_sort	attribution and identification of absorbed components by hplc-dad-esi-ms after oral administration of erhuang decoction
title_auth	Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction
abstract	Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.
abstractGer	Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.
abstract_unstemmed	Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.
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title_short	Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction
url	https://dx.doi.org/10.1186/s40543-020-00236-4
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author2	Zheng, Dandan Xu, Nan Zhang, Chao Wang, Yingzi Sun, Xiumei Zhang, Zhaowang
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up_date	2024-07-03T18:18:54.919Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">SPR04079654X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230519092424.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">201007s2020 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s40543-020-00236-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR04079654X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s40543-020-00236-4-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">ASE</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">30.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">50.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wang, Jinglong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract To realize the attribution and identification of absorbed components in rat serum after oral administration of Erhuang decoction prepared by semi-bionic enzyme extraction method, the fingerprints of serum samples were established using a HPLC-DAD-ESI-MS method. Thirty-two peaks in Erhuang decoction and 24 peaks in rat serum after oral administration of Erhuang decoction were detected. Among the 24 peaks detected in rat serum, 25 compounds were identified by comparing the retention time and mass spectrometry data with that of reference compounds, or by mass spectrometry analysis and retrieving the reference literatures. Among the identified 25 compounds in vivo, 24 were the original form of compounds absorbed from the detected compounds in vitro, and one was the metabolite compounds of licorice. By analyzing the mass spectrometry or ultraviolet absorption characteristics, other unidentified compounds in vivo were deduced to be the endogenous metabolites in serum or the original form and metabolites of the compounds existed in vivo. Results indicated that HPLC-DAD-ESI-MS is suitable for identifying the bioactive constituents in serum after oral administration of Erhuang decoction, and the findings would be beneficial to further research and development of the pharmacodynamic substance base of Erhuang decoction.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Erhuang decoction</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Semi-bionic enzyme extraction</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">HPLC-DAD-ESI-MS</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bioactive constituents</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Identification</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zheng, Dandan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Nan</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Chao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Yingzi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sun, Xiumei</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Zhaowang</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of analytical science and technology</subfield><subfield code="d">Taejŏn : SpringerOpen, 2010</subfield><subfield code="g">11(2020), 1 vom: 28. 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Attribution and identification of absorbed components by HPLC-DAD-ESI-MS after oral administration of Erhuang decoction

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