Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP
Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorp...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Sheng-Yan [verfasserIn] Shu, Mei [verfasserIn] Zhong, Chan [verfasserIn] Chen, Hu [verfasserIn] Bi, Yan [verfasserIn] Hou, Peng-Fei [verfasserIn] Wu, Guo-Ping [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Food analytical methods - New York, NY : Springer, 2008, 13(2020), 10 vom: 12. Juli, Seite 1983-1992 |
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| Übergeordnetes Werk: |
volume:13 ; year:2020 ; number:10 ; day:12 ; month:07 ; pages:1983-1992 |
| Links: |
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| DOI / URN: |
10.1007/s12161-020-01814-3 |
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| Katalog-ID: |
SPR040879550 |
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| 245 | 1 | 0 | |a Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
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| 520 | |a Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. | ||
| 650 | 4 | |a Bentonite-coated activated carbon (BCAC) |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Characterization |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Adsorption kinetics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Polymerase inhibitors |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rti-LAMP |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Shu, Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Chan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Hu |e verfasserin |4 aut | |
| 700 | 1 | |a Bi, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Hou, Peng-Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Guo-Ping |e verfasserin |4 aut | |
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10.1007/s12161-020-01814-3 doi (DE-627)SPR040879550 (SPR)s12161-020-01814-3-e DE-627 ger DE-627 rakwb eng 660 540 ASE Li, Sheng-Yan verfasserin aut Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 Shu, Mei verfasserin aut Zhong, Chan verfasserin aut Chen, Hu verfasserin aut Bi, Yan verfasserin aut Hou, Peng-Fei verfasserin aut Wu, Guo-Ping verfasserin aut Enthalten in Food analytical methods New York, NY : Springer, 2008 13(2020), 10 vom: 12. Juli, Seite 1983-1992 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 https://dx.doi.org/10.1007/s12161-020-01814-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2020 10 12 07 1983-1992 |
| spelling |
10.1007/s12161-020-01814-3 doi (DE-627)SPR040879550 (SPR)s12161-020-01814-3-e DE-627 ger DE-627 rakwb eng 660 540 ASE Li, Sheng-Yan verfasserin aut Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 Shu, Mei verfasserin aut Zhong, Chan verfasserin aut Chen, Hu verfasserin aut Bi, Yan verfasserin aut Hou, Peng-Fei verfasserin aut Wu, Guo-Ping verfasserin aut Enthalten in Food analytical methods New York, NY : Springer, 2008 13(2020), 10 vom: 12. Juli, Seite 1983-1992 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 https://dx.doi.org/10.1007/s12161-020-01814-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2020 10 12 07 1983-1992 |
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10.1007/s12161-020-01814-3 doi (DE-627)SPR040879550 (SPR)s12161-020-01814-3-e DE-627 ger DE-627 rakwb eng 660 540 ASE Li, Sheng-Yan verfasserin aut Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 Shu, Mei verfasserin aut Zhong, Chan verfasserin aut Chen, Hu verfasserin aut Bi, Yan verfasserin aut Hou, Peng-Fei verfasserin aut Wu, Guo-Ping verfasserin aut Enthalten in Food analytical methods New York, NY : Springer, 2008 13(2020), 10 vom: 12. Juli, Seite 1983-1992 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 https://dx.doi.org/10.1007/s12161-020-01814-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2020 10 12 07 1983-1992 |
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10.1007/s12161-020-01814-3 doi (DE-627)SPR040879550 (SPR)s12161-020-01814-3-e DE-627 ger DE-627 rakwb eng 660 540 ASE Li, Sheng-Yan verfasserin aut Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 Shu, Mei verfasserin aut Zhong, Chan verfasserin aut Chen, Hu verfasserin aut Bi, Yan verfasserin aut Hou, Peng-Fei verfasserin aut Wu, Guo-Ping verfasserin aut Enthalten in Food analytical methods New York, NY : Springer, 2008 13(2020), 10 vom: 12. Juli, Seite 1983-1992 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 https://dx.doi.org/10.1007/s12161-020-01814-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2020 10 12 07 1983-1992 |
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10.1007/s12161-020-01814-3 doi (DE-627)SPR040879550 (SPR)s12161-020-01814-3-e DE-627 ger DE-627 rakwb eng 660 540 ASE Li, Sheng-Yan verfasserin aut Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 Shu, Mei verfasserin aut Zhong, Chan verfasserin aut Chen, Hu verfasserin aut Bi, Yan verfasserin aut Hou, Peng-Fei verfasserin aut Wu, Guo-Ping verfasserin aut Enthalten in Food analytical methods New York, NY : Springer, 2008 13(2020), 10 vom: 12. Juli, Seite 1983-1992 (DE-627)566007320 (DE-600)2424728-5 1936-976X nnns volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 https://dx.doi.org/10.1007/s12161-020-01814-3 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 13 2020 10 12 07 1983-1992 |
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English |
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Enthalten in Food analytical methods 13(2020), 10 vom: 12. Juli, Seite 1983-1992 volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 |
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Enthalten in Food analytical methods 13(2020), 10 vom: 12. Juli, Seite 1983-1992 volume:13 year:2020 number:10 day:12 month:07 pages:1983-1992 |
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Bentonite-coated activated carbon (BCAC) Characterization Adsorption kinetics Polymerase inhibitors Rti-LAMP |
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Li, Sheng-Yan @@aut@@ Shu, Mei @@aut@@ Zhong, Chan @@aut@@ Chen, Hu @@aut@@ Bi, Yan @@aut@@ Hou, Peng-Fei @@aut@@ Wu, Guo-Ping @@aut@@ |
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In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. 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Li, Sheng-Yan |
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Li, Sheng-Yan ddc 660 misc Bentonite-coated activated carbon (BCAC) misc Characterization misc Adsorption kinetics misc Polymerase inhibitors misc Rti-LAMP Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
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660 540 ASE Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP Bentonite-coated activated carbon (BCAC) (dpeaa)DE-He213 Characterization (dpeaa)DE-He213 Adsorption kinetics (dpeaa)DE-He213 Polymerase inhibitors (dpeaa)DE-He213 Rti-LAMP (dpeaa)DE-He213 |
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ddc 660 misc Bentonite-coated activated carbon (BCAC) misc Characterization misc Adsorption kinetics misc Polymerase inhibitors misc Rti-LAMP |
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Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
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Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
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1983 |
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Li, Sheng-Yan Shu, Mei Zhong, Chan Chen, Hu Bi, Yan Hou, Peng-Fei Wu, Guo-Ping |
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Elektronische Aufsätze |
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Li, Sheng-Yan |
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10.1007/s12161-020-01814-3 |
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characterization and kinetic study of bentonite-coated activated carbon for adsorption of dna polymerase inhibitors to improve the detection sensitivity of salmonella derived from vegetables by rti-lamp |
| title_auth |
Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
| abstract |
Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. |
| abstractGer |
Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. |
| abstract_unstemmed |
Abstract Bentonite-coated activated carbon (BCAC) as an effective adsorbent can absorb soluble impurities in food to improve the detection sensitivity of Rti-LAMP assay. In the present study, BCAC was optimally prepared and characterized by SEM-EDS, FTIR, Raman spectrum, and $ pH_{PZC} $. The adsorptive kinetics and capacities of BCAC for four representatives, including indigo carmine (IC), tryptophan, thiamine, and tannic acid, were also determined. The results showed that the coating ratio between bentonite and 1.25–2 mm coconut shell activated carbon (AC) was 1:4 (g/g), which was the optimum process for the preparation of BCAC. The recovery rate of Salmonella was about 94.0 ± 8.9% after BCAC treatment. The lowest level of DNA consistent detection was that from 2 CFU/g Salmonella-contaminated lettuce and bean sprouts by the Rti-LAMP assay combined with BCAC treatment. The adsorption rate of BCAC to IC was 88.9%, which was much consistent with the pseudo-second-order kinetic as the R2 value of 0.993. The adsorption rate of BCAC to tryptophan was 86.4% in accordance with the pseudo-first-order kinetic equation as the R2 value of 0.957. Also, the adsorption rates of thiamine, tannic acid, and lettuce water-soluble compounds by BCAC were 83.1%, 24%, and 50%, respectively. The SEM-EDS image and FTIR analysis showed that the uniform bentonite layer was successfully coated on the porous structure of AC to form the BCAC. The Raman spectra and $ pH_{PZC} $ data showed that the internal structure and chemical properties of the BCAC were not different from those of AC. Therefore, the BCAC had a strong adsorption capacity for DNA polymerase inhibitors of vegetables, but not for bacterial cells, which would greatly improve the sensitivity of LAMP for detection of food pathogens. |
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Characterization and Kinetic Study of Bentonite-Coated Activated Carbon for Adsorption of DNA Polymerase Inhibitors to Improve the Detection Sensitivity of Salmonella Derived from Vegetables by Rti-LAMP |
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https://dx.doi.org/10.1007/s12161-020-01814-3 |
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| score |
7.4004526 |