Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model
Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhao, Lin [verfasserIn] Zhao, Junli [verfasserIn] Yu, Jia-Jia [verfasserIn] Zhang, Cangyu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Tissue Engineering and Regenerative Medicine - Springer Netherlands, 2012, 17(2020), 5 vom: 10. Sept., Seite 717-727 |
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| Übergeordnetes Werk: |
volume:17 ; year:2020 ; number:5 ; day:10 ; month:09 ; pages:717-727 |
| Links: |
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| DOI / URN: |
10.1007/s13770-020-00282-4 |
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| Katalog-ID: |
SPR041140281 |
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| 520 | |a Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. | ||
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| 650 | 4 | |a Periosteum |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Irregular bone |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Bone defect |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhao, Junli |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Jia-Jia |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Cangyu |e verfasserin |4 aut | |
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10.1007/s13770-020-00282-4 doi (DE-627)SPR041140281 (SPR)s13770-020-00282-4-e DE-627 ger DE-627 rakwb eng Zhao, Lin verfasserin aut Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. Tissue engineering (dpeaa)DE-He213 Periosteum (dpeaa)DE-He213 Irregular bone (dpeaa)DE-He213 Bone defect (dpeaa)DE-He213 Zhao, Junli verfasserin aut Yu, Jia-Jia verfasserin aut Zhang, Cangyu verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 17(2020), 5 vom: 10. Sept., Seite 717-727 (DE-627)SPR032345240 nnns volume:17 year:2020 number:5 day:10 month:09 pages:717-727 https://dx.doi.org/10.1007/s13770-020-00282-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 17 2020 5 10 09 717-727 |
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10.1007/s13770-020-00282-4 doi (DE-627)SPR041140281 (SPR)s13770-020-00282-4-e DE-627 ger DE-627 rakwb eng Zhao, Lin verfasserin aut Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. Tissue engineering (dpeaa)DE-He213 Periosteum (dpeaa)DE-He213 Irregular bone (dpeaa)DE-He213 Bone defect (dpeaa)DE-He213 Zhao, Junli verfasserin aut Yu, Jia-Jia verfasserin aut Zhang, Cangyu verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 17(2020), 5 vom: 10. Sept., Seite 717-727 (DE-627)SPR032345240 nnns volume:17 year:2020 number:5 day:10 month:09 pages:717-727 https://dx.doi.org/10.1007/s13770-020-00282-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 17 2020 5 10 09 717-727 |
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10.1007/s13770-020-00282-4 doi (DE-627)SPR041140281 (SPR)s13770-020-00282-4-e DE-627 ger DE-627 rakwb eng Zhao, Lin verfasserin aut Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. Tissue engineering (dpeaa)DE-He213 Periosteum (dpeaa)DE-He213 Irregular bone (dpeaa)DE-He213 Bone defect (dpeaa)DE-He213 Zhao, Junli verfasserin aut Yu, Jia-Jia verfasserin aut Zhang, Cangyu verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 17(2020), 5 vom: 10. Sept., Seite 717-727 (DE-627)SPR032345240 nnns volume:17 year:2020 number:5 day:10 month:09 pages:717-727 https://dx.doi.org/10.1007/s13770-020-00282-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 17 2020 5 10 09 717-727 |
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10.1007/s13770-020-00282-4 doi (DE-627)SPR041140281 (SPR)s13770-020-00282-4-e DE-627 ger DE-627 rakwb eng Zhao, Lin verfasserin aut Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. Tissue engineering (dpeaa)DE-He213 Periosteum (dpeaa)DE-He213 Irregular bone (dpeaa)DE-He213 Bone defect (dpeaa)DE-He213 Zhao, Junli verfasserin aut Yu, Jia-Jia verfasserin aut Zhang, Cangyu verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 17(2020), 5 vom: 10. Sept., Seite 717-727 (DE-627)SPR032345240 nnns volume:17 year:2020 number:5 day:10 month:09 pages:717-727 https://dx.doi.org/10.1007/s13770-020-00282-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 17 2020 5 10 09 717-727 |
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10.1007/s13770-020-00282-4 doi (DE-627)SPR041140281 (SPR)s13770-020-00282-4-e DE-627 ger DE-627 rakwb eng Zhao, Lin verfasserin aut Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. Tissue engineering (dpeaa)DE-He213 Periosteum (dpeaa)DE-He213 Irregular bone (dpeaa)DE-He213 Bone defect (dpeaa)DE-He213 Zhao, Junli verfasserin aut Yu, Jia-Jia verfasserin aut Zhang, Cangyu verfasserin aut Enthalten in Tissue Engineering and Regenerative Medicine Springer Netherlands, 2012 17(2020), 5 vom: 10. Sept., Seite 717-727 (DE-627)SPR032345240 nnns volume:17 year:2020 number:5 day:10 month:09 pages:717-727 https://dx.doi.org/10.1007/s13770-020-00282-4 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_21 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_40 GBV_ILN_61 GBV_ILN_65 GBV_ILN_69 GBV_ILN_130 GBV_ILN_285 AR 17 2020 5 10 09 717-727 |
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irregular bone defect repair using tissue-engineered periosteum in a rabbit model |
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Irregular Bone Defect Repair Using Tissue-Engineered Periosteum in a Rabbit Model |
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Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. |
| abstractGer |
Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. |
| abstract_unstemmed |
Background: In previous studies, we succeeded in repairing a long bone defect with tissue-engineered periosteum (TEP), fabricated by incorporating rabbit mesenchymal stem cells with small intestinal submucosa. In this study, we investigated the feasibility of allogeneic irregular bone defect repair using TEP. Methods: We performed a subtotal resection of the scapula in 36 rabbits to establish a large irregular bone defect model. The rabbits were then randomly divided into three groups (n = 12 per group) and the defects were treated with TEP (Group 1), allogeneic deproteinized bone (DPB) (Group 2) or a hybrid of TEP and DPB (Group 3). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed to detect bone healing. Ink-formaldehyde perfusion was introduced to qualitatively analyze vascularization in TEP engineered new bone. Results: The repair of scapular defects was diverse in all groups, shown by radiographic and histological tests. The radiographic scores in Group 1 and Group 3 were significantly higher than Group 2 at 8 and 12 weeks (p < 0.05). Histological scores further proved that Group 1 had significantly greater new bone formation compared to Group 3 (p < 0.05), while Group 2 had the lowest osteogenesis at all time-points (p < 0.001). Ink-formaldehyde perfusion revealed aboundant microvessels in TEP engineered new bone. Conclusion: We conclude that TEP is promising for the repair of large irregular bone defects. As a 3D scaffold, DPB could provide mechanical support and a shaping guide when combined with TEP. TEP engineered new bone has aboundant microvessels. |
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