Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyroph...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Sun, Heng [verfasserIn] Li, Juanjuan [verfasserIn] Song, Heyun [verfasserIn] Yang, Dong [verfasserIn] Deng, Xianbao [verfasserIn] Liu, Juan [verfasserIn] Wang, Yunmeng [verfasserIn] Ma, Junyu [verfasserIn] Xiong, Yaqian [verfasserIn] Liu, Yanling [verfasserIn] Yang, Mei [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: BMC plant biology - London : BioMed Central, 2001, 20(2020), 1 vom: 06. Okt. |
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| Übergeordnetes Werk: |
volume:20 ; year:2020 ; number:1 ; day:06 ; month:10 |
| Links: |
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| DOI / URN: |
10.1186/s12870-020-02666-z |
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| Katalog-ID: |
SPR041228774 |
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| 245 | 1 | 0 | |a Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed |
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| 520 | |a Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. | ||
| 650 | 4 | |a Lotus seed |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Starch biosynthesis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Nutritional composition |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a AGPase gene |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a AGPase activity |7 (dpeaa)DE-He213 | |
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| 700 | 1 | |a Deng, Xianbao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Juan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yunmeng |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Junyu |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Yaqian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yanling |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Mei |e verfasserin |4 aut | |
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10.1186/s12870-020-02666-z doi (DE-627)SPR041228774 (SPR)s12870-020-02666-z-e DE-627 ger DE-627 rakwb eng 580 610 ASE 44.41 bkl Sun, Heng verfasserin aut Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 Li, Juanjuan verfasserin aut Song, Heyun verfasserin aut Yang, Dong verfasserin aut Deng, Xianbao verfasserin aut Liu, Juan verfasserin aut Wang, Yunmeng verfasserin aut Ma, Junyu verfasserin aut Xiong, Yaqian verfasserin aut Liu, Yanling verfasserin aut Yang, Mei verfasserin aut Enthalten in BMC plant biology London : BioMed Central, 2001 20(2020), 1 vom: 06. Okt. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:20 year:2020 number:1 day:06 month:10 https://dx.doi.org/10.1186/s12870-020-02666-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 20 2020 1 06 10 |
| spelling |
10.1186/s12870-020-02666-z doi (DE-627)SPR041228774 (SPR)s12870-020-02666-z-e DE-627 ger DE-627 rakwb eng 580 610 ASE 44.41 bkl Sun, Heng verfasserin aut Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 Li, Juanjuan verfasserin aut Song, Heyun verfasserin aut Yang, Dong verfasserin aut Deng, Xianbao verfasserin aut Liu, Juan verfasserin aut Wang, Yunmeng verfasserin aut Ma, Junyu verfasserin aut Xiong, Yaqian verfasserin aut Liu, Yanling verfasserin aut Yang, Mei verfasserin aut Enthalten in BMC plant biology London : BioMed Central, 2001 20(2020), 1 vom: 06. Okt. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:20 year:2020 number:1 day:06 month:10 https://dx.doi.org/10.1186/s12870-020-02666-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 20 2020 1 06 10 |
| allfields_unstemmed |
10.1186/s12870-020-02666-z doi (DE-627)SPR041228774 (SPR)s12870-020-02666-z-e DE-627 ger DE-627 rakwb eng 580 610 ASE 44.41 bkl Sun, Heng verfasserin aut Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 Li, Juanjuan verfasserin aut Song, Heyun verfasserin aut Yang, Dong verfasserin aut Deng, Xianbao verfasserin aut Liu, Juan verfasserin aut Wang, Yunmeng verfasserin aut Ma, Junyu verfasserin aut Xiong, Yaqian verfasserin aut Liu, Yanling verfasserin aut Yang, Mei verfasserin aut Enthalten in BMC plant biology London : BioMed Central, 2001 20(2020), 1 vom: 06. Okt. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:20 year:2020 number:1 day:06 month:10 https://dx.doi.org/10.1186/s12870-020-02666-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 20 2020 1 06 10 |
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10.1186/s12870-020-02666-z doi (DE-627)SPR041228774 (SPR)s12870-020-02666-z-e DE-627 ger DE-627 rakwb eng 580 610 ASE 44.41 bkl Sun, Heng verfasserin aut Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 Li, Juanjuan verfasserin aut Song, Heyun verfasserin aut Yang, Dong verfasserin aut Deng, Xianbao verfasserin aut Liu, Juan verfasserin aut Wang, Yunmeng verfasserin aut Ma, Junyu verfasserin aut Xiong, Yaqian verfasserin aut Liu, Yanling verfasserin aut Yang, Mei verfasserin aut Enthalten in BMC plant biology London : BioMed Central, 2001 20(2020), 1 vom: 06. Okt. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:20 year:2020 number:1 day:06 month:10 https://dx.doi.org/10.1186/s12870-020-02666-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 20 2020 1 06 10 |
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10.1186/s12870-020-02666-z doi (DE-627)SPR041228774 (SPR)s12870-020-02666-z-e DE-627 ger DE-627 rakwb eng 580 610 ASE 44.41 bkl Sun, Heng verfasserin aut Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 Li, Juanjuan verfasserin aut Song, Heyun verfasserin aut Yang, Dong verfasserin aut Deng, Xianbao verfasserin aut Liu, Juan verfasserin aut Wang, Yunmeng verfasserin aut Ma, Junyu verfasserin aut Xiong, Yaqian verfasserin aut Liu, Yanling verfasserin aut Yang, Mei verfasserin aut Enthalten in BMC plant biology London : BioMed Central, 2001 20(2020), 1 vom: 06. Okt. (DE-627)335489060 (DE-600)2059868-3 1471-2229 nnns volume:20 year:2020 number:1 day:06 month:10 https://dx.doi.org/10.1186/s12870-020-02666-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA SSG-OPC-PHA SSG-OPC-ASE GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.41 ASE AR 20 2020 1 06 10 |
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Enthalten in BMC plant biology 20(2020), 1 vom: 06. Okt. volume:20 year:2020 number:1 day:06 month:10 |
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Lotus seed Starch biosynthesis Nutritional composition AGPase gene AGPase activity |
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Sun, Heng @@aut@@ Li, Juanjuan @@aut@@ Song, Heyun @@aut@@ Yang, Dong @@aut@@ Deng, Xianbao @@aut@@ Liu, Juan @@aut@@ Wang, Yunmeng @@aut@@ Ma, Junyu @@aut@@ Xiong, Yaqian @@aut@@ Liu, Yanling @@aut@@ Yang, Mei @@aut@@ |
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Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. 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Sun, Heng ddc 580 bkl 44.41 misc Lotus seed misc Starch biosynthesis misc Nutritional composition misc AGPase gene misc AGPase activity Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed |
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580 610 ASE 44.41 bkl Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed Lotus seed (dpeaa)DE-He213 Starch biosynthesis (dpeaa)DE-He213 Nutritional composition (dpeaa)DE-He213 AGPase gene (dpeaa)DE-He213 AGPase activity (dpeaa)DE-He213 |
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Sun, Heng Li, Juanjuan Song, Heyun Yang, Dong Deng, Xianbao Liu, Juan Wang, Yunmeng Ma, Junyu Xiong, Yaqian Liu, Yanling Yang, Mei |
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comprehensive analysis of agpase genes uncovers their potential roles in starch biosynthesis in lotus seed |
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Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed |
| abstract |
Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. |
| abstractGer |
Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. |
| abstract_unstemmed |
Background Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. |
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Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed |
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Li, Juanjuan Song, Heyun Yang, Dong Deng, Xianbao Liu, Juan Wang, Yunmeng Ma, Junyu Xiong, Yaqian Liu, Yanling Yang, Mei |
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