T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype

Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA S...
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Autor*in:	Aldridge, Jonathan [verfasserIn] Ekwall, Anna-Karin H. [verfasserIn] Mark, Linda [verfasserIn] Bergström, Beatrice [verfasserIn] Andersson, Kerstin [verfasserIn] Gjertsson, Inger [verfasserIn] Lundell, Anna-Carin [verfasserIn] Rudin, Anna [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Rheumatoid arthritis Th1 Th2 TPh TFh Fibroblast-like synoviocytes Cytokines Chemokines

Übergeordnetes Werk:	Enthalten in: Arthritis Research & Therapy - London : BioMed Central, 1999, 22(2020), 1 vom: 16. Okt.
Übergeordnetes Werk:	volume:22 ; year:2020 ; number:1 ; day:16 ; month:10

Links:	Volltext

DOI / URN:	10.1186/s13075-020-02349-y

Katalog-ID:	SPR041370295

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520			\|a Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation.
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author_variant	j a ja a k h e akh akhe l m lm b b bb k a ka i g ig a c l acl a r ar
matchkey_str	article:14786354:2020----::hleclsnyoillioptetwtremtiatrtsrmrlhv
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allfields	10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10
spelling	10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10
allfields_unstemmed	10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10
allfieldsGer	10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10
allfieldsSound	10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10
language	English
source	Enthalten in Arthritis Research & Therapy 22(2020), 1 vom: 16. Okt. volume:22 year:2020 number:1 day:16 month:10
sourceStr	Enthalten in Arthritis Research & Therapy 22(2020), 1 vom: 16. Okt. volume:22 year:2020 number:1 day:16 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Rheumatoid arthritis Th1 Th2 TPh TFh Fibroblast-like synoviocytes Cytokines Chemokines
dewey-raw	610
isfreeaccess_bool	true
container_title	Arthritis Research & Therapy
authorswithroles_txt_mv	Aldridge, Jonathan @@aut@@ Ekwall, Anna-Karin H. @@aut@@ Mark, Linda @@aut@@ Bergström, Beatrice @@aut@@ Andersson, Kerstin @@aut@@ Gjertsson, Inger @@aut@@ Lundell, Anna-Carin @@aut@@ Rudin, Anna @@aut@@
publishDateDaySort_date	2020-10-16T00:00:00Z
hierarchy_top_id	326646418
dewey-sort	3610
id	SPR041370295
language_de	englisch
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In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. 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author	Aldridge, Jonathan
spellingShingle	Aldridge, Jonathan ddc 610 misc Rheumatoid arthritis misc Th1 misc Th2 misc TPh misc TFh misc Fibroblast-like synoviocytes misc Cytokines misc Chemokines T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
authorStr	Aldridge, Jonathan
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topic_title	610 ASE T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213
topic	ddc 610 misc Rheumatoid arthritis misc Th1 misc Th2 misc TPh misc TFh misc Fibroblast-like synoviocytes misc Cytokines misc Chemokines
topic_unstemmed	ddc 610 misc Rheumatoid arthritis misc Th1 misc Th2 misc TPh misc TFh misc Fibroblast-like synoviocytes misc Cytokines misc Chemokines
topic_browse	ddc 610 misc Rheumatoid arthritis misc Th1 misc Th2 misc TPh misc TFh misc Fibroblast-like synoviocytes misc Cytokines misc Chemokines
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title	T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
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title_full	T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
author_sort	Aldridge, Jonathan
journal	Arthritis Research & Therapy
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author_browse	Aldridge, Jonathan Ekwall, Anna-Karin H. Mark, Linda Bergström, Beatrice Andersson, Kerstin Gjertsson, Inger Lundell, Anna-Carin Rudin, Anna
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author-letter	Aldridge, Jonathan
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title_sort	helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a th1 and a $ cxcr3^{+} $th2 phenotype
title_auth	T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
abstract	Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation.
abstractGer	Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation.
abstract_unstemmed	Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation.
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container_issue	1
title_short	T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
url	https://dx.doi.org/10.1186/s13075-020-02349-y
remote_bool	true
author2	Ekwall, Anna-Karin H. Mark, Linda Bergström, Beatrice Andersson, Kerstin Gjertsson, Inger Lundell, Anna-Carin Rudin, Anna
author2Str	Ekwall, Anna-Karin H. Mark, Linda Bergström, Beatrice Andersson, Kerstin Gjertsson, Inger Lundell, Anna-Carin Rudin, Anna
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up_date	2024-07-03T21:46:49.112Z
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