T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype
Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA S...
Ausführliche Beschreibung
Autor*in: |
Aldridge, Jonathan [verfasserIn] Ekwall, Anna-Karin H. [verfasserIn] Mark, Linda [verfasserIn] Bergström, Beatrice [verfasserIn] Andersson, Kerstin [verfasserIn] Gjertsson, Inger [verfasserIn] Lundell, Anna-Carin [verfasserIn] Rudin, Anna [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2020 |
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Übergeordnetes Werk: |
Enthalten in: Arthritis Research & Therapy - London : BioMed Central, 1999, 22(2020), 1 vom: 16. Okt. |
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Übergeordnetes Werk: |
volume:22 ; year:2020 ; number:1 ; day:16 ; month:10 |
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DOI / URN: |
10.1186/s13075-020-02349-y |
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Katalog-ID: |
SPR041370295 |
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245 | 1 | 2 | |a T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype |
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520 | |a Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. | ||
650 | 4 | |a Rheumatoid arthritis |7 (dpeaa)DE-He213 | |
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700 | 1 | |a Ekwall, Anna-Karin H. |e verfasserin |4 aut | |
700 | 1 | |a Mark, Linda |e verfasserin |4 aut | |
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700 | 1 | |a Andersson, Kerstin |e verfasserin |4 aut | |
700 | 1 | |a Gjertsson, Inger |e verfasserin |4 aut | |
700 | 1 | |a Lundell, Anna-Carin |e verfasserin |4 aut | |
700 | 1 | |a Rudin, Anna |e verfasserin |4 aut | |
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10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10 |
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10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10 |
allfields_unstemmed |
10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10 |
allfieldsGer |
10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10 |
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10.1186/s13075-020-02349-y doi (DE-627)SPR041370295 (SPR)s13075-020-02349-y-e DE-627 ger DE-627 rakwb eng 610 ASE Aldridge, Jonathan verfasserin aut T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 Ekwall, Anna-Karin H. verfasserin aut Mark, Linda verfasserin aut Bergström, Beatrice verfasserin aut Andersson, Kerstin verfasserin aut Gjertsson, Inger verfasserin aut Lundell, Anna-Carin verfasserin aut Rudin, Anna verfasserin aut Enthalten in Arthritis Research & Therapy London : BioMed Central, 1999 22(2020), 1 vom: 16. Okt. (DE-627)326646418 (DE-600)2041668-4 1478-6354 nnns volume:22 year:2020 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13075-020-02349-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2020 1 16 10 |
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In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). 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Aldridge, Jonathan ddc 610 misc Rheumatoid arthritis misc Th1 misc Th2 misc TPh misc TFh misc Fibroblast-like synoviocytes misc Cytokines misc Chemokines T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype |
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610 ASE T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype Rheumatoid arthritis (dpeaa)DE-He213 Th1 (dpeaa)DE-He213 Th2 (dpeaa)DE-He213 TPh (dpeaa)DE-He213 TFh (dpeaa)DE-He213 Fibroblast-like synoviocytes (dpeaa)DE-He213 Cytokines (dpeaa)DE-He213 Chemokines (dpeaa)DE-He213 |
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helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a th1 and a $ cxcr3^{+} $th2 phenotype |
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T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype |
abstract |
Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. |
abstractGer |
Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. |
abstract_unstemmed |
Background The majority of $ CD4^{+} $ T helper (Th) cells found in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) express CXCR3, a receptor associated with Th1 cells. In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). TPh and PD-$ 1^{high} $TFh in RA SF were primarily of a Th1 and a $ CXCR3^{+} $Th2 phenotype. Moreover, the levels of CXCL9, CXCL10, CCL20, CCL2, CXCL8, IL-6 and IL-10 were higher in SF than in plasma (P < 0.05). Lastly, IL-4, IL-13 and IL-17A induced RA FLS to secrete proinflammatory IL-6, CCL2, CXCL1 and CXCL8, while IFNγ mainly induced CXCL10. Conclusion These findings indicate that not only Th1 but also $ CXCR3^{+} $Th2 cells may have a pathogenic role in RA synovial inflammation. |
collection_details |
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container_issue |
1 |
title_short |
T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a $ CXCR3^{+} $Th2 phenotype |
url |
https://dx.doi.org/10.1186/s13075-020-02349-y |
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author2 |
Ekwall, Anna-Karin H. Mark, Linda Bergström, Beatrice Andersson, Kerstin Gjertsson, Inger Lundell, Anna-Carin Rudin, Anna |
author2Str |
Ekwall, Anna-Karin H. Mark, Linda Bergström, Beatrice Andersson, Kerstin Gjertsson, Inger Lundell, Anna-Carin Rudin, Anna |
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doi_str |
10.1186/s13075-020-02349-y |
up_date |
2024-07-03T21:46:49.112Z |
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In blood, subsets of Th2 and Th17 cells also express CXCR3, but it is unknown if these cells are present in RA SF or how cytokines from these subsets affect cytokine/chemokine secretion by fibroblast-like synoviocytes (FLS) from patients with RA. Methods We examined the proportions of Th1, Th2, $ CXCR3^{+} $Th2, Th17, $ CXCR3^{+} $Th17, Th1Th17, peripheral T helper (TPh) and T follicular helper (TFh) cells in paired SF and blood, as well as the phenotype of TPh and TFh cells in RA SF (n = 8), by the use of flow cytometry. We also examined the cytokine/chemokine profile in paired SF and plasma (n = 8) and in culture supernatants of FLS from patients with chronic RA (n = 7) stimulated with Th-associated cytokines, by the use of cytometric bead arrays and ELISA. Cytokine receptor expression in FLS (n = 3) were assessed by the use of RNA sequencing and qPCR. Results The proportions of Th1 and $ CXCR3^{+} $Th2 cells were higher in SF than in blood (P < 0.05). 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