Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells

Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE...
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Autor*in:	Ru, Liyan [verfasserIn] Wu, Nan [verfasserIn] Wei, Keyu [verfasserIn] Zeng, Yuxiao [verfasserIn] Li, Qiyou [verfasserIn] Weng, Chuanhuang [verfasserIn] Ren, Chunge [verfasserIn] Ren, Bangqi [verfasserIn] Huo, Da [verfasserIn] Li, Yijian [verfasserIn] Hu, Xisu [verfasserIn] Qin, Zuoxin [verfasserIn] Fang, Yajie [verfasserIn] Zhu, Chuhong [verfasserIn] Liu, Yong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Retinal pigment epithelial Tissue engineering Encapsulation Retinal degenerative diseases

Übergeordnetes Werk:	Enthalten in: Stem cell research & therapy - London : BioMed Central, 2010, 11(2020), 1 vom: 25. Nov.
Übergeordnetes Werk:	volume:11 ; year:2020 ; number:1 ; day:25 ; month:11

Links:	Volltext

DOI / URN:	10.1186/s13287-020-01986-z

Katalog-ID:	SPR042150744

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520			\|a Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation.
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allfields	10.1186/s13287-020-01986-z doi (DE-627)SPR042150744 (SPR)s13287-020-01986-z-e DE-627 ger DE-627 rakwb eng 570 610 ASE Ru, Liyan verfasserin aut Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation. Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213 Wu, Nan verfasserin aut Wei, Keyu verfasserin aut Zeng, Yuxiao verfasserin aut Li, Qiyou verfasserin aut Weng, Chuanhuang verfasserin aut Ren, Chunge verfasserin aut Ren, Bangqi verfasserin aut Huo, Da verfasserin aut Li, Yijian verfasserin aut Hu, Xisu verfasserin aut Qin, Zuoxin verfasserin aut Fang, Yajie verfasserin aut Zhu, Chuhong verfasserin aut Liu, Yong verfasserin aut Enthalten in Stem cell research & therapy London : BioMed Central, 2010 11(2020), 1 vom: 25. Nov. (DE-627)624251047 (DE-600)2548671-8 1757-6512 nnns volume:11 year:2020 number:1 day:25 month:11 https://dx.doi.org/10.1186/s13287-020-01986-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 25 11
spelling	10.1186/s13287-020-01986-z doi (DE-627)SPR042150744 (SPR)s13287-020-01986-z-e DE-627 ger DE-627 rakwb eng 570 610 ASE Ru, Liyan verfasserin aut Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation. Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213 Wu, Nan verfasserin aut Wei, Keyu verfasserin aut Zeng, Yuxiao verfasserin aut Li, Qiyou verfasserin aut Weng, Chuanhuang verfasserin aut Ren, Chunge verfasserin aut Ren, Bangqi verfasserin aut Huo, Da verfasserin aut Li, Yijian verfasserin aut Hu, Xisu verfasserin aut Qin, Zuoxin verfasserin aut Fang, Yajie verfasserin aut Zhu, Chuhong verfasserin aut Liu, Yong verfasserin aut Enthalten in Stem cell research & therapy London : BioMed Central, 2010 11(2020), 1 vom: 25. Nov. (DE-627)624251047 (DE-600)2548671-8 1757-6512 nnns volume:11 year:2020 number:1 day:25 month:11 https://dx.doi.org/10.1186/s13287-020-01986-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 25 11
allfields_unstemmed	10.1186/s13287-020-01986-z doi (DE-627)SPR042150744 (SPR)s13287-020-01986-z-e DE-627 ger DE-627 rakwb eng 570 610 ASE Ru, Liyan verfasserin aut Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation. Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213 Wu, Nan verfasserin aut Wei, Keyu verfasserin aut Zeng, Yuxiao verfasserin aut Li, Qiyou verfasserin aut Weng, Chuanhuang verfasserin aut Ren, Chunge verfasserin aut Ren, Bangqi verfasserin aut Huo, Da verfasserin aut Li, Yijian verfasserin aut Hu, Xisu verfasserin aut Qin, Zuoxin verfasserin aut Fang, Yajie verfasserin aut Zhu, Chuhong verfasserin aut Liu, Yong verfasserin aut Enthalten in Stem cell research & therapy London : BioMed Central, 2010 11(2020), 1 vom: 25. Nov. (DE-627)624251047 (DE-600)2548671-8 1757-6512 nnns volume:11 year:2020 number:1 day:25 month:11 https://dx.doi.org/10.1186/s13287-020-01986-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 25 11
allfieldsGer	10.1186/s13287-020-01986-z doi (DE-627)SPR042150744 (SPR)s13287-020-01986-z-e DE-627 ger DE-627 rakwb eng 570 610 ASE Ru, Liyan verfasserin aut Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation. Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213 Wu, Nan verfasserin aut Wei, Keyu verfasserin aut Zeng, Yuxiao verfasserin aut Li, Qiyou verfasserin aut Weng, Chuanhuang verfasserin aut Ren, Chunge verfasserin aut Ren, Bangqi verfasserin aut Huo, Da verfasserin aut Li, Yijian verfasserin aut Hu, Xisu verfasserin aut Qin, Zuoxin verfasserin aut Fang, Yajie verfasserin aut Zhu, Chuhong verfasserin aut Liu, Yong verfasserin aut Enthalten in Stem cell research & therapy London : BioMed Central, 2010 11(2020), 1 vom: 25. Nov. (DE-627)624251047 (DE-600)2548671-8 1757-6512 nnns volume:11 year:2020 number:1 day:25 month:11 https://dx.doi.org/10.1186/s13287-020-01986-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 25 11
allfieldsSound	10.1186/s13287-020-01986-z doi (DE-627)SPR042150744 (SPR)s13287-020-01986-z-e DE-627 ger DE-627 rakwb eng 570 610 ASE Ru, Liyan verfasserin aut Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation. Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213 Wu, Nan verfasserin aut Wei, Keyu verfasserin aut Zeng, Yuxiao verfasserin aut Li, Qiyou verfasserin aut Weng, Chuanhuang verfasserin aut Ren, Chunge verfasserin aut Ren, Bangqi verfasserin aut Huo, Da verfasserin aut Li, Yijian verfasserin aut Hu, Xisu verfasserin aut Qin, Zuoxin verfasserin aut Fang, Yajie verfasserin aut Zhu, Chuhong verfasserin aut Liu, Yong verfasserin aut Enthalten in Stem cell research & therapy London : BioMed Central, 2010 11(2020), 1 vom: 25. Nov. (DE-627)624251047 (DE-600)2548671-8 1757-6512 nnns volume:11 year:2020 number:1 day:25 month:11 https://dx.doi.org/10.1186/s13287-020-01986-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 25 11
language	English
source	Enthalten in Stem cell research & therapy 11(2020), 1 vom: 25. Nov. volume:11 year:2020 number:1 day:25 month:11
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authorswithroles_txt_mv	Ru, Liyan @@aut@@ Wu, Nan @@aut@@ Wei, Keyu @@aut@@ Zeng, Yuxiao @@aut@@ Li, Qiyou @@aut@@ Weng, Chuanhuang @@aut@@ Ren, Chunge @@aut@@ Ren, Bangqi @@aut@@ Huo, Da @@aut@@ Li, Yijian @@aut@@ Hu, Xisu @@aut@@ Qin, Zuoxin @@aut@@ Fang, Yajie @@aut@@ Zhu, Chuhong @@aut@@ Liu, Yong @@aut@@
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author	Ru, Liyan
spellingShingle	Ru, Liyan ddc 570 misc Retinal pigment epithelial misc Tissue engineering misc Encapsulation misc Retinal degenerative diseases Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells
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topic_title	570 610 ASE Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells Retinal pigment epithelial (dpeaa)DE-He213 Tissue engineering (dpeaa)DE-He213 Encapsulation (dpeaa)DE-He213 Retinal degenerative diseases (dpeaa)DE-He213
topic	ddc 570 misc Retinal pigment epithelial misc Tissue engineering misc Encapsulation misc Retinal degenerative diseases
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author_browse	Ru, Liyan Wu, Nan Wei, Keyu Zeng, Yuxiao Li, Qiyou Weng, Chuanhuang Ren, Chunge Ren, Bangqi Huo, Da Li, Yijian Hu, Xisu Qin, Zuoxin Fang, Yajie Zhu, Chuhong Liu, Yong
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title_sort	improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hesc-derived rpe cells
title_auth	Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells
abstract	Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation.
abstractGer	Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation.
abstract_unstemmed	Background Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation.
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title_short	Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells
url	https://dx.doi.org/10.1186/s13287-020-01986-z
remote_bool	true
author2	Wu, Nan Wei, Keyu Zeng, Yuxiao Li, Qiyou Weng, Chuanhuang Ren, Chunge Ren, Bangqi Huo, Da Li, Yijian Hu, Xisu Qin, Zuoxin Fang, Yajie Zhu, Chuhong Liu, Yong
author2Str	Wu, Nan Wei, Keyu Zeng, Yuxiao Li, Qiyou Weng, Chuanhuang Ren, Chunge Ren, Bangqi Huo, Da Li, Yijian Hu, Xisu Qin, Zuoxin Fang, Yajie Zhu, Chuhong Liu, Yong
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doi_str	10.1186/s13287-020-01986-z
up_date	2024-07-04T01:02:21.562Z
_version_	1803608330409607168
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However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Methods Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. Results This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. Conclusions We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. 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Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells

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